Restriction map for pF1KA2015
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AgeI        AhdI        Alw44I      ApaI        ApaLI       
AscI        Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BbrPI       BbvCI       BciVI       
BglI        BlnI        BlpI        BpmI        Bpu1102I    
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BspHI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstXI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoO109I    
EcoO65I     FseI        FspI        HindIII     HpaI        
KpnI        MluI        MroI        NaeI        NgoMIV      
NheI        NotI        NruI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SalI        SbfI        
ScaI        SexAI       SfcI        SfiI        SgrAI       
SmaI        SphI        Sse8387I    StuI        SwaI        
Tth111I     Van91I      XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AflII        2413  CTTAAG               C^TTAAG
AflIII        689  ACATGT               A^C[GA][CT]GT
AlwI          111  GATCC                GGATC(4/5)
AlwNI        1731  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1530  CCTAAGG              CC^T[ACGT]AGG
Aor51HI       152  AGCGCT               AGC^GCT
AspHI          48  GAGCTC               G[AT]GC[AT]^C
AvaI         1436  CTCGGG               C^[CT]CG[GA]G
BanI          370  GGCGCC               G^G[CT][GA]CC
BanII          48  GAGCTC               G[GA]GC[CT]^C
BbeI          370  GGCGCC               GGCGC^C
BbsI         2280  GTCTTC               GAAGAC(2/6)
BcgI          708  CGAGGATTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2762  TGATCA               T^GATCA
BfrI         2413  CTTAAG               C^TTAAG
BglII        1758  AGATCT               A^GATCT
BpuAI        2280  GTCTTC               GAAGAC(2/6)
BsaAI         671  TACGTA               [CT]AC^GT[GA]
BsaBI         279  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI        1627  GAGGAG               GAGGAG(10/8)
BsiHKAI        48  GAGCTC               G[AT]GC[AT]^C
BsmBI          24  GAGACG               CGTCTC(1/5)
BsmI         1191  GAATGC               GAATGC(1/-1)
BsoBI        1436  CTCGGG               C^[CT]CG[GA]G
BspLU11I      689  ACATGT               A^CATGT
BspMI         167  GCAGGT               ACCTGC(4/8)
BsrDI         999  CATTGC               GCAATG(2/0)
BstBI         791  TTCGAA               TT^CGAA
Bsu36I       1530  CCTAAGG              CC^T[ACGT]AGG
DraI         1806  TTTAAA               TTT^AAA
Eco47III      152  AGCGCT               AGC^GCT
Eco81I       1530  CCTAAGG              CC^T[ACGT]AGG
EcoNI          30  CCTGGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         391  GATATC               GAT^ATC
FbaI         2762  TGATCA               T^GATCA
KasI          370  GGCGCC               G^GCGCC
KspI          140  CCGCGG               CCGC^GG
MamI          279  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2178  CAATTG               C^AATTG
MunI         2178  CAATTG               C^AATTG
NarI          370  GGCGCC               GG^CGCC
NspV          791  TTCGAA               TT^CGAA
PvuII        1522  CAGCTG               CAG^CTG
SacI           48  GAGCTC               GAGCT^C
SacII         140  CCGCGG               CCGC^GG
SapI            9  GCTCTTC              GCTCTTC(1/4)
SfoI          370  GGCGCC               GGC^GCC
SfuI          791  TTCGAA               TT^CGAA
SnaBI         671  TACGTA               TAC^GTA
SpeI         1329  ACTAGT               A^CTAGT
XcmI          350  CCATCGTTCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BalI          696  TGGCCA               TGG^CCA
             2300  TGGCCA              
BmyI           48  GAGCTC               G[AGT]GC[ACT]^C
              227  GTGCCC              
BsgI          262  CTGCAC               GTGCAG(16/14)
             2491  GTGCAG              
Bsp1286I       48  GAGCTC               G[AGT]GC[ACT]^C
              227  GTGCCC              
BstYI         110  AGATCC               [GA]^GATC[CT]
             1758  AGATCT              
EcoT22I      1570  ATGCAT               ATGCA^T
             2156  ATGCAT              
FauI          188  CCCGC                CCCGC(4/6)
               70  GCGGG               
HincII        586  GTTGAC               GT[CT]^[GA]AC
             1640  GTCAAC              
HindII        586  GTTGAC               GT[CT]^[GA]AC
             1640  GTCAAC              
MflI          110  AGATCC               [GA]^GATC[CT]
             1758  AGATCT              
MluNI         696  TGGCCA               TGG^CCA
             2300  TGGCCA              
MscI          696  TGGCCA               TGG^CCA
             2300  TGGCCA              
NdeI          541  CATATG               CA^TATG
             2422  CATATG              
NsiI         1570  ATGCAT               ATGCA^T
             2156  ATGCAT              
NspI          579  ACATGC               [GA]CATG^[CT]
              689  ACATGT              
PleI         1121  GACTC                GAGTC(4/5)
             1915  GAGTC               
TfiI          937  GATTC                G^A[AT]TC
             1258  GAATC               
XbaI         1357  TCTAGA               T^CTAGA
             1764  TCTAGA              
XhoII         110  AGATCC               [GA]^GATC[CT]
             1758  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        ApoI        AseI        AsnI        Asp700I     
AvaII       BbvI        BcnI        BfaI        BsaHI       
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1407I    BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EaeI        EarI        
EcoRI       EcoRII      EcoT14I     Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PshBI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        SspBI       SspI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XmnI        
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