Restriction map for
pF1KA2009
(Circular) (Six-base) MAPSORT of: pF1KA2009.seq Check: 6194 from: 1 to: 4820
pF1KA2009 4820 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1858 1858
Size: 4820
Acc65I G'GTAC_C
Cuts at: 606 1836 606
Size: 1230 3590
AclI AA'CG_TT
Cuts at: 4660 4660
Size: 4820
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2645 3077 3430 4092 2645
Size: 432 353 662 3373
Fragments arranged by size:
3373 662 432 353
AfeI AGC'GCT
Cuts at: 380 380
Size: 4820
AflIII A'CryG_T
Cuts at: 680 1574 2172 3972 4253 680
Size: 894 598 1800 281 1247
Fragments arranged by size:
1800 1247 894 598 281
AleI CACnn'nnGTG
Cuts at: 847 847
Size: 4820
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 703 735 703
Size: 32 4788
AlwNI CAG_nnn'CTG
Cuts at: 899 1050 2125 3563 899
Size: 151 1075 1438 2156
Fragments arranged by size:
2156 1438 1075 151
ApaI G_GGCC'C
Cuts at: 641 1242 641
Size: 601 4219
ApaLI G'TGCA_C
Cuts at: 1390 3658 1390
Size: 2268 2552
ApoI r'AATT_y
Cuts at: 1824 4173 4429 1824
Size: 2349 256 2215
Fragments arranged by size:
2349 2215 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4820
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4820
AvaI C'yCGr_G
Cuts at: 580 758 1177 1257 1342 1452 1497 1762 1840
Size: 178 419 80 85 110 45 265 78
Cuts at: 1840 580
Size: 3560
Fragments arranged by size:
3560 419 265 178 110 85 80 78
45
BamHI G'GATC_C
Cuts at: 1845 1845
Size: 4820
BanI G'GyrC_C
Cuts at: 259 558 606 646 841 1224 1351 1411 1698
Size: 299 48 40 195 383 127 60 287
Cuts at: 1698 1836 2250 2498 2533 259
Size: 138 414 248 35 2546
Fragments arranged by size:
2546 414 383 299 287 248 195 138
127 60 48 40 35
BanII G_rGCy'C
Cuts at: 207 629 641 812 1242 1585 1763 1834 2864
Size: 422 12 171 430 343 178 71 1030
Cuts at: 2864 207
Size: 2163
Fragments arranged by size:
2163 1030 430 422 343 178 171 71
12
BbeI G_GCGC'C
Cuts at: 263 650 1355 2254 2502 263
Size: 387 705 899 248 2581
Fragments arranged by size:
2581 899 705 387 248
BbsI GAAGACnn'nnnn_
Cuts at: 397 1329 397
Size: 932 3888
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1669 1703 90
Size: 34 1545 34 3207
Fragments arranged by size:
3207 1545 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2718 3774 4820 2718
Size: 1056 1046 2718
Fragments arranged by size:
2718 1056 1046
BclI T'GATC_A
Cuts at: 2340 2340
Size: 4820
BglI GCCn_nnn'nGGC
Cuts at: 1456 1456
Size: 4820
BglII A'GATC_T
Cuts at: 2335 4372 2335
Size: 2037 2783
BlpI GC'TnA_GC
Cuts at: 1932 1932
Size: 4820
Bme1580I G_kGCm'C
Cuts at: 641 1227 1242 1394 1548 1703 1771 2445 2538
Size: 586 15 152 154 155 68 674 93
Cuts at: 2538 3662 641
Size: 1124 1799
Fragments arranged by size:
1799 1124 674 586 155 154 152 93
68 15
BmgBI CAC'GTC
Cuts at: 1577 1577
Size: 4820
BmrI ACTGGGnnnn_n'
Cuts at: 320 409 1236 2032 2113 2448 4592 320
Size: 89 827 796 81 335 2144 548
Fragments arranged by size:
2144 827 796 548 335 89 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 279 507 525 3187 279
Size: 228 18 2662 1912
Fragments arranged by size:
2662 1912 228 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 921 957 1593 154
Size: 767 36 636 3381
Fragments arranged by size:
3381 767 636 36
Bpu10I CC'TnA_GC
Cuts at: 1081 1081
Size: 4820
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1996 2875 3589 3887 1996
Size: 879 714 298 2929
Fragments arranged by size:
2929 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 178 4524 178
Size: 4346 474
BsaAI yAC'GTr
Cuts at: 683 2173 683
Size: 1490 3330
BsaBI GATnn'nnATC
Cuts at: 6 863 2334 4371 6
Size: 857 1471 2037 455
Fragments arranged by size:
2037 1471 857 455
BsaHI Gr'CG_yC
Cuts at: 260 647 694 1352 2251 2499 3201 4697 260
Size: 387 47 658 899 248 702 1496 383
Fragments arranged by size:
1496 899 702 658 387 383 248 47
BsaWI w'CCGG_w
Cuts at: 1278 2009 2530 3208 3619 3766 4040 1278
Size: 731 521 678 411 147 274 2058
Fragments arranged by size:
2058 731 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 214 244 703 733 955 985 214
Size: 30 459 30 222 30 4049
Fragments arranged by size:
4049 459 222 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 442 778 1317 1520 1575 1578 1593 1596 442
Size: 336 539 203 55 3 15 3 3666
Fragments arranged by size:
3666 539 336 203 55 15 3 3
BseYI C'CCAG_C
Cuts at: 112 237 697 916 1043 1539 2245 3003 3668
Size: 125 460 219 127 496 706 758 665
Cuts at: 3668 4076 112
Size: 408 856
Fragments arranged by size:
856 758 706 665 496 460 408 219
127 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1387 1387
Size: 4820
BsiEI CG_ry'CG
Cuts at: 104 939 2362 2408 3638 104
Size: 835 1423 46 1230 1286
Fragments arranged by size:
1423 1286 1230 835 46
BsiHKAI G_wGCw'C
Cuts at: 506 812 1394 1469 1834 2612 2802 3662 506
Size: 306 582 75 365 778 190 860 1664
Fragments arranged by size:
1664 860 778 582 365 306 190 75
Bsp1286I G_dGCh'C
Cuts at: 207 506 629 641 812 1227 1242 1394 1469
Size: 299 123 12 171 415 15 152 75
Cuts at: 1469 1548 1585 1703 1763 1771 1834 2445 2538
Size: 79 37 118 60 8 63 611 93
Cuts at: 2538 2612 2802 2864 3662 207
Size: 74 190 62 798 1365
Fragments arranged by size:
1365 798 611 415 299 190 171 152
123 118 93 79 75 74 63 62
60 37 15 12 8
BspHI T'CATG_A
Cuts at: 3252 3252
Size: 4820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 584 1278 1870 2084 2386 2767 584
Size: 694 592 214 302 381 2637
Fragments arranged by size:
2637 694 592 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1210 1251 3112 3166 4650 4815 50
Size: 1160 41 1861 54 1484 165 55
Fragments arranged by size:
1861 1484 1160 165 55 54 41
BsrDI GCAATG_nn'
Cuts at: 779 1125 2732 779
Size: 346 1607 2867
Fragments arranged by size:
2867 1607 346
BsrFI r'CCGG_y
Cuts at: 714 730 793 1397 2818 2999 714
Size: 16 63 604 1421 181 2535
Fragments arranged by size:
2535 1421 604 181 63 16
BssSI C'ACGA_G
Cuts at: 628 3091 3799 628
Size: 2463 708 1649
Fragments arranged by size:
2463 1649 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1418 1730 2197 1418
Size: 312 467 4041
Fragments arranged by size:
4041 467 312
BstBI TT'CG_AA
Cuts at: 3181 3181
Size: 4820
BstEII G'GTnAC_C
Cuts at: 726 726
Size: 4820
BstXI CCAn_nnnn'nTGG
Cuts at: 1235 1539 1235
Size: 304 4516
BstYI r'GATC_y
Cuts at: 1 1845 2312 2335 2670 2916 3222 3234 3320
Size: 1844 467 23 335 246 306 12 86
Cuts at: 3320 4372 1
Size: 1052 449
Fragments arranged by size:
1844 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 470 1331 1784 470
Size: 861 453 3506
Fragments arranged by size:
3506 861 453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2770 2951 3114 2770
Size: 181 163 4476
Fragments arranged by size:
4476 181 163
BtsI GCAGTG_nn'
Cuts at: 993 2063 993
Size: 1070 3750
DraI TTT'AAA
Cuts at: 1819 1819
Size: 4820
DrdI GACnn_nn'nnGTC
Cuts at: 2526 3870 2526
Size: 1344 3476
EaeI y'GGCC_r
Cuts at: 467 936 1707 2405 2579 2970 2997 4342 467
Size: 469 771 698 174 391 27 1345 945
Fragments arranged by size:
1345 945 771 698 469 391 174 27
EagI C'GGCC_G
Cuts at: 936 2405 936
Size: 1469 3351
EarI CTCTTCn'nnn_
Cuts at: 286 1302 1596 1620 1629 1723 1747 2843 3053
Size: 1016 294 24 9 94 24 1096 210
Cuts at: 3053 286
Size: 2053
Fragments arranged by size:
2053 1096 1016 294 210 94 24 24
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1069 1280 3028 3770 3916 4630 1069
Size: 211 1748 742 146 714 1259
Fragments arranged by size:
1748 1259 742 714 211 146
EcoICRI GAG'CTC
Cuts at: 810 1832 810
Size: 1022 3798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 921 957 1593 2645 3077 3430 4092 154
Size: 767 36 636 1052 432 353 662 882
Fragments arranged by size:
1052 882 767 662 636 432 353 36
EcoO109I rG'GnC_Cy
Cuts at: 637 1959 637
Size: 1322 3498
EcoRI G'AATT_C
Cuts at: 1824 1824
Size: 4820
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 4820
FspI TGC'GCA
Cuts at: 1091 1489 2601 1091
Size: 398 1112 3310
Fragments arranged by size:
3310 1112 398
HaeII r_GCGC'y
Cuts at: 263 382 650 1050 1355 2254 2502 3732 4503
Size: 119 268 400 305 899 248 1230 771
Cuts at: 4503 263
Size: 580
Fragments arranged by size:
1230 899 771 580 400 305 268 248
119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 214 246 2662 2692 2694 2724 214
Size: 32 2416 30 2 30 2310
Fragments arranged by size:
2416 2310 32 30 30 2
HincII GTy'rAC
Cuts at: 1859 1859
Size: 4820
KasI G'GCGC_C
Cuts at: 259 646 1351 2250 2498 259
Size: 387 705 899 248 2581
Fragments arranged by size:
2581 899 705 387 248
KpnI G_GTAC'C
Cuts at: 610 1840 610
Size: 1230 3590
MluI A'CGCG_T
Cuts at: 4253 4253
Size: 4820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1391 1550 3579 3763 1391
Size: 159 2029 184 2448
Fragments arranged by size:
2448 2029 184 159
MscI TGG'CCA
Cuts at: 469 1709 2581 469
Size: 1240 872 2708
Fragments arranged by size:
2708 1240 872
MslI CAynn'nnrTG
Cuts at: 598 847 2809 2936 598
Size: 249 1962 127 2482
Fragments arranged by size:
2482 1962 249 127
MspA1I CmG'CkG
Cuts at: 149 173 1036 1138 1418 1551 1786 1931 2245
Size: 24 863 102 280 133 235 145 314
Cuts at: 2245 2605 3389 3634 149
Size: 360 784 245 1335
Fragments arranged by size:
1335 863 784 360 314 280 245 235
145 133 102 24
NaeI GCC'GGC
Cuts at: 1399 3001 1399
Size: 1602 3218
NarI GG'CG_CC
Cuts at: 260 647 1352 2251 2499 260
Size: 387 705 899 248 2581
Fragments arranged by size:
2581 899 705 387 248
NcoI C'CATG_G
Cuts at: 470 470
Size: 4820
NgoMIV G'CCGG_C
Cuts at: 1397 2999 1397
Size: 1602 3218
NspI r_CATG'y
Cuts at: 340 738 1873 2373 3976 340
Size: 398 1135 500 1603 1184
Fragments arranged by size:
1603 1184 1135 500 398
PciI A'CATG_T
Cuts at: 3972 3972
Size: 4820
PflMI CCAn_nnn'nTGG
Cuts at: 1050 1050
Size: 4820
PfoI T'CCnGG_A
Cuts at: 526 526
Size: 4820
PmeI GTTT'AAAC
Cuts at: 1819 1819
Size: 4820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 703 735 3266 3298 703
Size: 32 2531 32 2225
Fragments arranged by size:
2531 2225 32 32
PspOMI G'GGCC_C
Cuts at: 637 1238 637
Size: 601 4219
PstI C_TGCA'G
Cuts at: 212 1187 1275 1867 2552 212
Size: 975 88 592 685 2480
Fragments arranged by size:
2480 975 685 592 88
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4820
PvuII CAG'CTG
Cuts at: 1418 1551 2245 2605 1418
Size: 133 694 360 3633
Fragments arranged by size:
3633 694 360 133
RsrII CG'GwC_CG
Cuts at: 3015 3015
Size: 4820
SacI G_AGCT'C
Cuts at: 812 1834 812
Size: 1022 3798
SacII CC_GC'GG
Cuts at: 1787 1787
Size: 4820
SalI G'TCGA_C
Cuts at: 1857 1857
Size: 4820
SapI GCTCTTCn'nnn_
Cuts at: 286 1302 1620 1747 2843 3053 286
Size: 1016 318 127 1096 210 2053
Fragments arranged by size:
2053 1096 1016 318 210 127
SbfI CC_TGCA'GG
Cuts at: 1867 1867
Size: 4820
ScaI AGT'ACT
Cuts at: 4337 4337
Size: 4820
SfcI C'TryA_G
Cuts at: 33 208 1059 1183 1271 1863 2548 3516 3707
Size: 175 851 124 88 592 685 968 191
Cuts at: 3707 33
Size: 1146
Fragments arranged by size:
1146 968 851 685 592 191 175 124
88
SfoI GGC'GCC
Cuts at: 261 648 1353 2252 2500 261
Size: 387 705 899 248 2581
Fragments arranged by size:
2581 899 705 387 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4820
SmaI CCC'GGG
Cuts at: 582 1259 1344 1454 1499 1842 582
Size: 677 85 110 45 343 3560
Fragments arranged by size:
3560 677 343 110 85 45
SmlI C'TyrA_G
Cuts at: 758 1975 2890 3604 3866 758
Size: 1217 915 714 262 1712
Fragments arranged by size:
1712 1217 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4820
SphI G_CATG'C
Cuts at: 738 1873 2373 738
Size: 1135 500 3185
Fragments arranged by size:
3185 1135 500
SrfI GCCC'GGGC
Cuts at: 582 582
Size: 4820
StuI AGG'CCT
Cuts at: 1511 1511
Size: 4820
StyI C'CwwG_G
Cuts at: 470 1954 2308 470
Size: 1484 354 2982
Fragments arranged by size:
2982 1484 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 322 2403 2536 2995 3204 322
Size: 2081 133 459 209 1938
Fragments arranged by size:
2081 1938 459 209 133
TatI w'GTAC_w
Cuts at: 4335 4335
Size: 4820
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4337 4369 4337
Size: 32 4788
Tth111I GACn'n_nGTC
Cuts at: 2617 2617
Size: 4820
XbaI T'CTAG_A
Cuts at: 1851 1851
Size: 4820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 896 1374 2315 896
Size: 478 941 3401
Fragments arranged by size:
3401 941 478
XhoI C'TCGA_G
Cuts at: 758 758
Size: 4820
XmaI C'CCGG_G
Cuts at: 580 1257 1342 1452 1497 1840 580
Size: 677 85 110 45 343 3560
Fragments arranged by size:
3560 677 343 110 85 45
XmnI GAAnn'nnTTC
Cuts at: 826 826
Size: 4820
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AlfI AscI AvrII
BaeI BbvCI BfrBI BmtI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SspI SwaI ZraI