Restriction map for pF1KA1913
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       BalI        
BamHI       BbrPI       BcgI        BciVI       BfrI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaWI       BseAI       BsgI        BsiWI       BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BssHII      
BssSI       Bst1107I    BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoT22I     FauI        
FseI        FspI        HgaI        HpaI        KpnI        
Ksp632I     KspI        MaeII       MamI        MluI        
MluNI       MroI        MscI        MvnI        NdeI        
NheI        NotI        NruI        NsiI        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PstI        PvuII       
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   66 enzymes) #
###################################

AcsI          960  GAATTT               [GA]^AATT[CT]
AcyI          122  GGCGCC               G[GA]^CG[CT]C
AlwI         1266  GGATC                GGATC(4/5)
ApoI          960  GAATTT               [GA]^AATT[CT]
AvrII        1258  CCTAGG               C^CTAGG
BanI          122  GGCGCC               G^G[CT][GA]CC
BbeI          122  GGCGCC               GGCGC^C
BbvCI          81  CCTCAGC              CCTCAGC(-5/-2)
BclI          875  TGATCA               T^GATCA
BglI          114  GCCCATCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1393  AGATCT               A^GATCT
BlnI         1258  CCTAGG               C^CTAGG
BsaHI         122  GGCGCC               G[GA]^CG[CT]C
BsaI          460  GAGACC               GGTCTC(1/5)
BseRI         679  GAGGAG               GAGGAG(10/8)
BsiEI         626  CGATCG               CG[GA][CT]^CG
BsmBI          89  CGTCTC               CGTCTC(1/5)
BsmFI        1208  GTCCC                GGGAC(10/14)
BsmI          360  GCATTC               GAATGC(1/-1)
Bsp1407I      551  TGTACA               T^GTACA
BspHI         441  TCATGA               T^CATGA
BsrDI         866  CATTGC               GCAATG(2/0)
BsrFI         125  GCCGGC               [GA]^CCGG[CT]
BsrGI         551  TGTACA               T^GTACA
BstBI         659  TTCGAA               TT^CGAA
BstYI        1393  AGATCT               [GA]^GATC[CT]
Cac8I         125  GCCGGC               GC[ACGT]^[ACGT]GC
CfoI          123  GCGC                 GCG^C
Cfr10I        125  GCCGGC               [GA]^CCGG[CT]
DraII        1206  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoNI          38  CCTTGAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1206  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRV         484  GATATC               GAT^ATC
FbaI          875  TGATCA               T^GATCA
HaeII         122  GGCGCC               [GA]GCGC^[CT]
HhaI          123  GCGC                 GCG^C
Hin1I         122  GGCGCC               G[GA]^CG[CT]C
HinP1I        123  GCGC                 G^CGC
HindIII      1375  AAGCTT               A^AGCTT
HphI          392  TCACC                GGTGA(8/7)
KasI          122  GGCGCC               G^GCGCC
MfeI          836  CAATTG               C^AATTG
MflI         1393  AGATCT               [GA]^GATC[CT]
MslI          471  CATACACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        807  CAGCGG               C[AC]G^C[GT]G
MunI          836  CAATTG               C^AATTG
NaeI          125  GCCGGC               GCC^GGC
NarI          122  GGCGCC               GG^CGCC
NcoI          395  CCATGG               C^CATGG
NgoMIV        125  GCCGGC               G^CCGGC
NspV          659  TTCGAA               TT^CGAA
PaeR7I        606  CTCGAG               C^TCGAG
PpuMI        1206  GGGTCCC              [GA]G^G[AT]CC[CT]
PshAI        1201  GACCGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI          626  CGATCG               CGAT^CG
RcaI          441  TCATGA               T^CATGA
SacI          594  GAGCTC               GAGCT^C
SfoI          122  GGCGCC               GGC^GCC
SfuI          659  TTCGAA               TT^CGAA
SmaI          131  CCCGGG               CCC^GGG
SmlI          606  CTCGAG               C^T[CT][GA]AG
SspBI         551  TGTACA               T^GTACA
XhoI          606  CTCGAG               C^TCGAG
XhoII        1393  AGATCT               [GA]^GATC[CT]
XmaCI         131  CCCGGG               C^CCGGG
XmaI          131  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          552  GTAC                 GT^AC
             1074  GTAC                
AspHI         205  GTGCTC               G[AT]GC[AT]^C
              594  GAGCTC              
BanII         594  GAGCTC               G[GA]GC[CT]^C
              904  GAGCCC              
BbsI         1136  GAAGAC               GAAGAC(2/6)
             1309  GAAGAC              
BfaI         1259  CTAG                 C^TAG
             1297  CTAG                
BpuAI        1136  GAAGAC               GAAGAC(2/6)
             1309  GAAGAC              
BsiHKAI       205  GTGCTC               G[AT]GC[AT]^C
              594  GAGCTC              
BstAPI        351  GCAGCATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              720  GCATCTTATGC         
DsaI          395  CCATGG               C^C[GA][CT]GG
              674  CCGTGG              
EaeI           32  TGGCCG               [CT]^GGCC[GA]
              233  TGGCCG              
HincII         76  GTCAAC               GT[CT]^[GA]AC
              291  GTCAAC              
HindII         76  GTCAAC               GT[CT]^[GA]AC
              291  GTCAAC              
MaeI         1259  CTAG                 C^TAG
             1297  CTAG                
MseI          697  TTAA                 T^TAA
              884  TTAA                
NspI           72  GCATGT               [GA]CATG^[CT]
              548  GCATGT              
RsaI          552  GTAC                 GT^AC
             1074  GTAC                
Tru9I         697  TTAA                 T^TAA
              884  TTAA                
Tsp45I        452  GTGAC                ^GT[GC]AC
             1176  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AluI        AvaI        AvaII       BbvI        
BcnI        BmyI        BsaJI       BsiYI       BslI        
BsmAI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cfr13I      DdeI        DpnII       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       ItaI        MaeIII      
MboI        MboII       MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TaqI        TfiI        TseI        Tsp509I     
TspRI       TthHB8I     
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