Restriction map for pF1KA1838
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# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AvaI        
AvrII       BamHI       BbeI        BbvCI       BcgI        
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrFI       BssHII      BstBI       
BstEII      BstPI       CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FbaI        FseI        HpaI        
KasI        KpnI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmeI        PpuMI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SwaI        
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccII        2332  CGCG                 CG^CG
AcyI         1000  GACGCC               G[GA]^CG[CT]C
AflIII       1171  ACGTGT               A^C[GA][CT]GT
AhdI         1877  GACAGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          401  CCTCAGG              CC^T[ACGT]AGG
Asp700I      1015  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1877  GACAGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        2229  TGCGCA               TGC^GCA
BalI          183  TGGCCA               TGG^CCA
BanI         1976  GGCACC               G^G[CT][GA]CC
BbrPI        1170  CACGTG               CAC^GTG
BcnI         1980  CCCGG                CC^[GC]GG
BglI          680  GCCTCCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2491  AGATCT               A^GATCT
BsaAI        1170  CACGTG               [CT]AC^GT[GA]
BsaHI        1000  GACGCC               G[GA]^CG[CT]C
BsaI         2678  GAGACC               GGTCTC(1/5)
BseRI        2112  CTCCTC               GAGGAG(10/8)
BsgI         2308  GTGCAG               GTGCAG(16/14)
BsmI         1804  GAATGC               GAATGC(1/-1)
BspHI        1784  TCATGA               T^CATGA
BssSI         990  CACGAG               CACGAG(-5/-1)
BstAPI       2534  GCAAGGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        2332  CGCG                 CG^CG
Bsu36I        401  CCTCAGG              CC^T[ACGT]AGG
Eam1105I     1877  GACAGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        401  CCTCAGG              CC^T[ACGT]AGG
EcoRV         333  GATATC               GAT^ATC
FauI         1881  GCGGG                CCCGC(4/6)
FspI         2229  TGCGCA               TGC^GCA
HaeII        2075  GGCGCT               [GA]GCGC^[CT]
HapII        1981  CCGG                 C^CGG
Hin1I        1000  GACGCC               G[GA]^CG[CT]C
HincII       2356  GTCAAC               GT[CT]^[GA]AC
HindII       2356  GTCAAC               GT[CT]^[GA]AC
HpaII        1981  CCGG                 C^CGG
KspI         2331  CCGCGG               CCGC^GG
MluNI         183  TGGCCA               TGG^CCA
MscI          183  TGGCCA               TGG^CCA
MspI         1981  CCGG                 C^CGG
MvnI         2332  CGCG                 CG^CG
NciI         1980  CCCGG                CC^[GC]GG
NdeI         1527  CATATG               CA^TATG
NheI          408  GCTAGC               G^CTAGC
PmaCI        1170  CACGTG               CAC^GTG
PmlI         1170  CACGTG               CAC^GTG
PshAI         556  GACACTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1784  TCATGA               T^CATGA
SacI          583  GAGCTC               GAGCT^C
SacII        2331  CCGCGG               CCGC^GG
SapI         2122  GAAGAGC              GCTCTTC(1/4)
ScaI         2438  AGTACT               AGT^ACT
SexAI        1769  ACCTGGT              A^CC[AT]GGT
SphI         2217  GCATGC               GCATG^C
SspI         2037  AATATT               AAT^ATT
XmnI         1015  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AspHI          82  GAGCAC               G[AT]GC[AT]^C
              583  GAGCTC              
AspI          604  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1984  GACCTCGTC           
BciVI          99  GTATCC               GTATCC(6/5)
             1956  GTATCC              
BsiHKAI        82  GAGCAC               G[AT]GC[AT]^C
              583  GAGCTC              
BsmAI        2678  GAGAC                GTCTC(1/5)
              143  GTCTC               
BspMI          37  ACCTGC               ACCTGC(4/8)
             1927  ACCTGC              
BstF5I        396  CATCC                GGATG(2/0)
              106  GGATG               
BstXI         163  CCAGAATCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1670  CCACAAATGTGG        
FokI          396  CATCC                GGATG(9/13)
              106  GGATG               
HgaI         1000  GACGC                GACGC(5/10)
             2558  GACGC               
MaeII        1171  ACGT                 A^CGT
             1751  ACGT                
NcoI          934  CCATGG               C^CATGG
             1706  CCATGG              
PflFI         604  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1984  GACCTCGTC           
PvuII         239  CAGCTG               CAG^CTG
             2311  CAGCTG              
SmlI          316  CTCAAG               C^T[CT][GA]AG
             2115  CTCAAG              
Tsp45I        360  GTCAC                ^GT[GC]AC
             1918  GTCAC               
Tth111I       604  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1984  GACCTCGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AvaII       BanII       
BbsI        BbvI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BsiYI       BslI        BsmFI       
Bsp1286I    Bsp1407I    BsrGI       BsrI        BssKI       
Bst1107I    BstNI       BstYI       BstZ17I     Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      HaeIII      HhaI        HinP1I      
HindIII     HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SspBI       StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XhoII       
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