Restriction map for pF1KA1838
 (Linear) (Six-base) MAPSORT of: KIAA1838.seq  Check: 3956  from: 1  to: 2730



KIAA1838.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1936    2730 
   Size:       1936     794
 
AccI GT'mk_AC

Cuts at:      0     157    1102    1282    1318    1354    1390    1426    1886 
   Size:        157     945     180      36      36      36      36     460

Cuts at:   1886    2730 
   Size:        844

  Fragments arranged by size: 

                 945     844     460     180     157      36      36      36
                  36
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1277    1706    2539    2730 
   Size:       1277     429     833     191

  Fragments arranged by size: 

                1277     833     429     191
 
AflIII A'CryG_T

Cuts at:      0    1171    2730 
   Size:       1171    1559
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1882    2730 
   Size:       1882     848
 
AleI CACnn'nnGTG

Cuts at:      0    1675    2730 
   Size:       1675    1055
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2547    2730 
   Size:       2547     183
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1039    1075    1111    1147    1183    1219    1255    1291 
   Size:       1039      36      36      36      36      36      36      36

Cuts at:   1291    1327    1363    1399    1435    1471    1720    1928    2730 
   Size:         36      36      36      36      36     249     208     802

  Fragments arranged by size: 

                1039     802     249     208      36      36      36      36
                  36      36      36      36      36      36      36      36
            
 
ApoI r'AATT_y

Cuts at:      0      63    1615    2506    2730 
   Size:         63    1552     891     224

  Fragments arranged by size: 

                1552     891     224      63
 
BanI G'GyrC_C

Cuts at:      0    1976    2730 
   Size:       1976     754
 
BanII G_rGCy'C

Cuts at:      0     587    2320    2577    2587    2620    2641    2730 
   Size:        587    1733     257      10      33      21      89

  Fragments arranged by size: 

                1733     587     257      89      33      21      10
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     539    1562    1670    2389    2616    2730 
   Size:        539    1023     108     719     227     114

  Fragments arranged by size: 

                1023     719     539     227     114     108
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     110    1967    2730 
   Size:        110    1857     763

  Fragments arranged by size: 

                1857     763     110
 
BglI GCCn_nnn'nGGC

Cuts at:      0     686    2730 
   Size:        686    2044
 
BglII A'GATC_T

Cuts at:      0    2491    2730 
   Size:       2491     239
 
BmgBI CAC'GTC

Cuts at:      0    1752    2730 
   Size:       1752     978
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     451    1909    2589    2730 
   Size:        451    1458     680     141

  Fragments arranged by size: 

                1458     680     451     141
 
BmtI G_CTAG'C

Cuts at:      0     412    2730 
   Size:        412    2318
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     646    2730 
   Size:        646    2084
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     487    2227    2604    2730 
   Size:        146     341    1740     377     126

  Fragments arranged by size: 

                1740     377     341     146     126
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301    2100    2730 
   Size:        301    1799     630

  Fragments arranged by size: 

                1799     630     301
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2672    2730 
   Size:       2672      58
 
BsaAI yAC'GTr

Cuts at:      0    1172    2730 
   Size:       1172    1558
 
BsaHI Gr'CG_yC

Cuts at:      0    1001    2730 
   Size:       1001    1729
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2103    2730 
   Size:       2103     627
 
BseYI C'CCAG_C

Cuts at:      0     241    1846    2313    2730 
   Size:        241    1605     467     417

  Fragments arranged by size: 

                1605     467     417     241
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2329    2730 
   Size:       2329     401
 
BsiHKAI G_wGCw'C

Cuts at:      0      86     587    2730 
   Size:         86     501    2143

  Fragments arranged by size: 

                2143     501      86
 
BsmI GAATG_Cn'

Cuts at:      0    1810    2730 
   Size:       1810     920
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     587    2320    2577    2587    2620    2641    2730 
   Size:         86     501    1733     257      10      33      21      89

  Fragments arranged by size: 

                1733     501     257      89      86      33      21      10
            
 
BspHI T'CATG_A

Cuts at:      0    1784    2730 
   Size:       1784     946
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      46    1936    2730 
   Size:         46    1890     794

  Fragments arranged by size: 

                1890     794      46
 
BsrGI T'GTAC_A

Cuts at:      0      52    1066    1174    1246    1495    1531    1567    1639 
   Size:         52    1014     108      72     249      36      36      72

Cuts at:   1639    1775    2730 
   Size:        136     955

  Fragments arranged by size: 

                1014     955     249     136     108      72      72      52
                  36      36
 
BssSI C'ACGA_G

Cuts at:      0     990    2730 
   Size:        990    1740
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2540    2730 
   Size:       2540     190
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     170    1677    2730 
   Size:        170    1507    1053

  Fragments arranged by size: 

                1507    1053     170
 
BstYI r'GATC_y

Cuts at:      0     173    1536    2491    2730 
   Size:        173    1363     955     239

  Fragments arranged by size: 

                1363     955     239     173
 
BstZ17I GTA'TAC

Cuts at:      0     158    1103    1283    1319    1355    1391    1427    2730 
   Size:        158     945     180      36      36      36      36    1303

  Fragments arranged by size: 

                1303     945     180     158      36      36      36      36
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     402    2730 
   Size:        402    2328
 
BtgI C'CryG_G

Cuts at:      0     934    1706    2331    2671    2730 
   Size:        934     772     625     340      59

  Fragments arranged by size: 

                 934     772     625     340      59
 
BtsI GCAGTG_nn'

Cuts at:      0    1797    2730 
   Size:       1797     933
 
EaeI y'GGCC_r

Cuts at:      0     183     656    2568    2730 
   Size:        183     473    1912     162

  Fragments arranged by size: 

                1912     473     183     162
 
EarI CTCTTCn'nnn_

Cuts at:      0     674     848    2117    2730 
   Size:        674     174    1269     613

  Fragments arranged by size: 

                1269     674     613     174
 
EcoICRI GAG'CTC

Cuts at:      0     585    2730 
   Size:        585    2145
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     487    1277    1706    2227    2539    2604    2730 
   Size:        146     341     790     429     521     312      65     126

  Fragments arranged by size: 

                 790     521     429     341     312     146     126      65
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1072    1180    1252    1396    1432    1681    2730 
   Size:       1072     108      72     144      36     249    1049

  Fragments arranged by size: 

                1072    1049     249     144     108      72      36
 
EcoRV GAT'ATC

Cuts at:      0     335    2730 
   Size:        335    2395
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1846    2730 
   Size:       1846     884
 
FspI TGC'GCA

Cuts at:      0    2231    2730 
   Size:       2231     499
 
HaeII r_GCGC'y

Cuts at:      0    2079    2730 
   Size:       2079     651
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     796     828     849     881     991    1023    1027    1059 
   Size:        796      32      21      32     110      32       4      32

Cuts at:   1059    1459    1491    1528    1560    1868    1900    2483    2515 
   Size:        400      32      37      32     308      32     583      32

Cuts at:   2515    2730 
   Size:        215

  Fragments arranged by size: 

                 796     583     400     308     215     110      37      32
                  32      32      32      32      32      32      32      21
                   4
 
HincII GTy'rAC

Cuts at:      0    2358    2730 
   Size:       2358     372
 
HindIII A'AGCT_T

Cuts at:      0     720    2153    2422    2730 
   Size:        720    1433     269     308

  Fragments arranged by size: 

                1433     720     308     269
 
MscI TGG'CCA

Cuts at:      0     185    2730 
   Size:        185    2545
 
MslI CAynn'nnrTG

Cuts at:      0      48     610    1675    2730 
   Size:         48     562    1065    1055

  Fragments arranged by size: 

                1065    1055     562      48
 
MspA1I CmG'CkG

Cuts at:      0     241    1881    1998    2313    2333    2730 
   Size:        241    1640     117     315      20     397

  Fragments arranged by size: 

                1640     397     315     241     117      20
 
NcoI C'CATG_G

Cuts at:      0     934    1706    2730 
   Size:        934     772    1024

  Fragments arranged by size: 

                1024     934     772
 
NdeI CA'TA_TG

Cuts at:      0    1528    2730 
   Size:       1528    1202
 
NheI G'CTAG_C

Cuts at:      0     408    2730 
   Size:        408    2322
 
NspI r_CATG'y

Cuts at:      0     714    2221    2369    2730 
   Size:        714    1507     148     361

  Fragments arranged by size: 

                1507     714     361     148
 
PfoI T'CCnGG_A

Cuts at:      0     102     337    2730 
   Size:        102     235    2393

  Fragments arranged by size: 

                2393     235     102
 
PmlI CAC'GTG

Cuts at:      0    1172    2730 
   Size:       1172    1558
 
PshAI GACnn'nnGTC

Cuts at:      0     560    2730 
   Size:        560    2170
 
PsiI TTA'TAA

Cuts at:      0     893    2730 
   Size:        893    1837
 
PstI C_TGCA'G

Cuts at:      0     622    2003    2132    2730 
   Size:        622    1381     129     598

  Fragments arranged by size: 

                1381     622     598     129
 
PvuII CAG'CTG

Cuts at:      0     241    2313    2730 
   Size:        241    2072     417

  Fragments arranged by size: 

                2072     417     241
 
SacI G_AGCT'C

Cuts at:      0     587    2730 
   Size:        587    2143
 
SacII CC_GC'GG

Cuts at:      0    2334    2730 
   Size:       2334     396
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2117    2730 
   Size:       2117     613
 
ScaI AGT'ACT

Cuts at:      0    2440    2730 
   Size:       2440     290
 
SexAI A'CCwGG_T

Cuts at:      0    1769    2730 
   Size:       1769     961
 
SfcI C'TryA_G

Cuts at:      0     618    1598    1999    2128    2278    2730 
   Size:        618     980     401     129     150     452

  Fragments arranged by size: 

                 980     618     452     401     150     129
 
SmlI C'TyrA_G

Cuts at:      0     316    2115    2730 
   Size:        316    1799     615

  Fragments arranged by size: 

                1799     615     316
 
SphI G_CATG'C

Cuts at:      0    2221    2730 
   Size:       2221     509
 
SspI AAT'ATT

Cuts at:      0    2039    2730 
   Size:       2039     691
 
StuI AGG'CCT

Cuts at:      0    1840    1855    2236    2539    2730 
   Size:       1840      15     381     303     191

  Fragments arranged by size: 

                1840     381     303     191      15
 
StyI C'CwwG_G

Cuts at:      0     684     934    1706    2247    2730 
   Size:        684     250     772     541     483

  Fragments arranged by size: 

                 772     684     541     483     250
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     291    2730 
   Size:        291    2439
 
TatI w'GTAC_w

Cuts at:      0      52    1066    1174    1246    1495    1531    1567    1639 
   Size:         52    1014     108      72     249      36      36      72

Cuts at:   1639    1775    2438    2730 
   Size:        136     663     292

  Fragments arranged by size: 

                1014     663     292     249     136     108      72      72
                  52      36      36
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     165     197     442     474    1736    1768    2373    2405 
   Size:        165      32     245      32    1262      32     605      32

Cuts at:   2405    2730 
   Size:        325

  Fragments arranged by size: 

                1262     605     325     245     165      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0     607    1987    2730 
   Size:        607    1380     743

  Fragments arranged by size: 

                1380     743     607
 
XmnI GAAnn'nnTTC

Cuts at:      0    1019    2730 
   Size:       1019    1711
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AhdI     AleI     AlfI    AlwNI
     ApoI     BanI    BanII     BbsI    BciVI     BglI    BglII    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaHI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrGI    BssSI   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
     BtsI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NspI     PfoI     PmlI    PshAI     PsiI
     PstI    PvuII     SacI    SacII     SapI     ScaI    SexAI     SfcI
     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AloI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI     BclI    BfrBI     BlpI Bme1580I   Bpu10I
    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI
    BsrDI    BsrFI   BssHII    BstBI   BstEII    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRI     FseI
    FspAI     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SwaI     XbaI     XcmI     XhoI
     XmaI     ZraI
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