Restriction map for pF1KA1796
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanII       BbeI        BbrPI       BbsI        BcgI        
BciVI       BfaI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsoBI       BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       Cfr10I      Cfr13I      ClaI        CpoI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT22I     FauI        FseI        FspI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MluI        MluNI       MroI        MscI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    StuI        SwaI        
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI         1068  GTCTAC               GT^[AC][GT]AC
AccII          11  CGCG                 CG^CG
AflII         541  CTTAAG               C^TTAAG
AflIII        184  ACGTGT               A^C[GA][CT]GT
AlwNI          42  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       142  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI          69  CCTCAGC              CCTCAGC(-5/-2)
BclI           59  TGATCA               T^GATCA
BcnI           99  CCGGG                CC^[GC]GG
BfrI          541  CTTAAG               C^TTAAG
BmyI         1026  GGGCAC               G[AGT]GC[ACT]^C
BpmI           76  CTGGAG               CTGGAG(16/14)
BseRI          52  GAGGAG               GAGGAG(10/8)
BsiEI          96  CGACCG               CG[GA][CT]^CG
BsmI          609  GAATGC               GAATGC(1/-1)
Bsp1286I     1026  GGGCAC               G[AGT]GC[ACT]^C
BspHI        1058  TCATGA               T^CATGA
BstUI          11  CGCG                 CG^CG
BstXI         311  CCACAAAATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         536  GCATGC               GC[ACGT]^[ACGT]GC
DraI          292  TTTAAA               TTT^AAA
EcoT14I       648  CCATGG               C^C[AT][AT]GG
FbaI           59  TGATCA               T^GATCA
HaeII         893  AGCGCC               [GA]GCGC^[CT]
HaeIII         81  GGCC                 GG^CC
HapII          99  CCGG                 C^CGG
HgaI          533  GACGC                GACGC(5/10)
HpaII          99  CCGG                 C^CGG
MaeIII        188  GTGAC                ^GT[ACGT]AC
MfeI          502  CAATTG               C^AATTG
MspI           99  CCGG                 C^CGG
MunI          502  CAATTG               C^AATTG
MvnI           11  CGCG                 CG^CG
MwoI           35  GCCCCACCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI           99  CCGGG                CC^[GC]GG
NcoI          648  CCATGG               C^CATGG
NspI          536  GCATGC               [GA]CATG^[CT]
PleI          190  GACTC                GAGTC(4/5)
PstI          693  CTGCAG               CTGCA^G
RcaI         1058  TCATGA               T^CATGA
SfaNI         680  GCATC                GCATC(5/9)
SfcI          693  CTGCAG               C^T[GA][CT]AG
SmlI          541  CTTAAG               C^T[CT][GA]AG
SphI          536  GCATGC               GCATG^C
SspI          578  AATATT               AAT^ATT
StyI          648  CCATGG               C^C[AT][AT]GG
TaqI          594  TCGA                 T^CGA
Tsp45I        188  GTGAC                ^GT[GC]AC
TthHB8I       594  TCGA                 T^CGA
XcmI          311  CCACAAAATTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          142  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BanI            6  GGTGCC               G^G[CT][GA]CC
             1027  GGCACC              
BsgI          468  CTGCAC               GTGCAG(16/14)
              400  GTGCAG              
BsmFI         101  GGGAC                GGGAC(10/14)
              806  GGGAC               
Bsp1407I      821  TGTACA               T^GTACA
              937  TGTACA              
BsrGI         821  TGTACA               T^GTACA
              937  TGTACA              
BstF5I        303  GGATG                GGATG(2/0)
              978  GGATG               
BstYI        1014  GGATCT               [GA]^GATC[CT]
             1089  AGATCC              
CfoI           12  GCGC                 GCG^C
              894  GCGC                
DdeI           70  CTCAG                C^T[ACGT]AG
              914  CTAAG               
EcoRI         463  GAATTC               G^AATTC
              703  GAATTC              
FokI          303  GGATG                GGATG(9/13)
              978  GGATG               
HhaI           12  GCGC                 GCG^C
              894  GCGC                
HinP1I         12  GCGC                 G^CGC
              894  GCGC                
MaeII         184  ACGT                 A^CGT
             1125  ACGT                
MflI         1014  GGATCT               [GA]^GATC[CT]
             1089  AGATCC              
MslI          298  CACAAGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              604  CATGGGAATG          
NlaIV           6  GGTGCC               GG[ACGT]^[ACGT]CC
             1027  GGCACC              
SspBI         821  TGTACA               T^GTACA
              937  TGTACA              
XhoII        1014  GGATCT               [GA]^GATC[CT]
             1089  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BbvI        BsaJI       BsiYI       BslI        
BsrI        BssKI       BstNI       DpnII       DsaI        
EcoRII      Fnu4HI      HinfI       HphI        ItaI        
MboI        MboII       MnlI        MseI        MspA1I      
MvaI        NdeII       NlaIII      PvuII       RsaI        
Sau3AI      ScrFI       TfiI        Tru9I       TseI        
Tsp509I     TspRI       
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