Restriction map for pF1KA1796
 (Linear) (Six-base) MAPSORT of: KIAA1796.seq  Check: 2490  from: 1  to: 1128



KIAA1796.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AccI GT'mk_AC

Cuts at:      0    1069    1128 
   Size:       1069      59
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     539     672    1128 
   Size:        539     133     456

  Fragments arranged by size: 

                 539     456     133
 
AflII C'TTAA_G

Cuts at:      0     541    1128 
   Size:        541     587
 
AflIII A'CryG_T

Cuts at:      0     184    1128 
   Size:        184     944
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     265     297    1128 
   Size:        265      32     831

  Fragments arranged by size: 

                 831     265      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      47    1128 
   Size:         47    1081
 
ApoI r'AATT_y

Cuts at:      0     289     463     703     880     909     925    1128 
   Size:        289     174     240     177      29      16     203

  Fragments arranged by size: 

                 289     240     203     177     174      29      16
 
BanI G'GyrC_C

Cuts at:      0       6    1027    1128 
   Size:          6    1021     101

  Fragments arranged by size: 

                1021     101       6
 
BbvCI CC'TCA_GC

Cuts at:      0      70    1128 
   Size:         70    1058
 
BclI T'GATC_A

Cuts at:      0      59    1128 
   Size:         59    1069
 
Bme1580I G_kGCm'C

Cuts at:      0    1030    1128 
   Size:       1030      98
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97    1128 
   Size:         97    1031
 
Bpu10I CC'TnA_GC

Cuts at:      0      70    1128 
   Size:         70    1058
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     265     295    1128 
   Size:        265      30     833

  Fragments arranged by size: 

                 833     265      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67    1128 
   Size:         67    1061
 
BseYI C'CCAG_C

Cuts at:      0    1093    1128 
   Size:       1093      35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     453    1128 
   Size:        421      32     675

  Fragments arranged by size: 

                 675     421      32
 
BsiEI CG_ry'CG

Cuts at:      0      99    1128 
   Size:         99    1029
 
BsmI GAATG_Cn'

Cuts at:      0     615    1128 
   Size:        615     513
 
Bsp1286I G_dGCh'C

Cuts at:      0    1030    1128 
   Size:       1030      98
 
BspHI T'CATG_A

Cuts at:      0    1058    1128 
   Size:       1058      70
 
BsrGI T'GTAC_A

Cuts at:      0     821     937    1128 
   Size:        821     116     191

  Fragments arranged by size: 

                 821     191     116
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     318    1128 
   Size:        318     810
 
BstYI r'GATC_y

Cuts at:      0    1014    1089    1128 
   Size:       1014      75      39

  Fragments arranged by size: 

                1014      75      39
 
BtgI C'CryG_G

Cuts at:      0     354     648    1031    1128 
   Size:        354     294     383      97

  Fragments arranged by size: 

                 383     354     294      97
 
BtsI GCAGTG_nn'

Cuts at:      0     404     824    1128 
   Size:        404     420     304

  Fragments arranged by size: 

                 420     404     304
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     302     337    1128 
   Size:        302      35     791

  Fragments arranged by size: 

                 791     302      35
 
DraI TTT'AAA

Cuts at:      0     294    1128 
   Size:        294     834
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     539     672    1128 
   Size:         97     442     133     456

  Fragments arranged by size: 

                 456     442     133      97
 
EcoRI G'AATT_C

Cuts at:      0     463     703    1128 
   Size:        463     240     425

  Fragments arranged by size: 

                 463     425     240
 
HaeII r_GCGC'y

Cuts at:      0     897    1128 
   Size:        897     231
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     208     240    1128 
   Size:        208      32     888

  Fragments arranged by size: 

                 888     208      32
 
MfeI C'AATT_G

Cuts at:      0     502    1128 
   Size:        502     626
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     488    1101    1128 
   Size:        488     613      27

  Fragments arranged by size: 

                 613     488      27
 
MslI CAynn'nnrTG

Cuts at:      0     302     608    1128 
   Size:        302     306     520

  Fragments arranged by size: 

                 520     306     302
 
MspA1I CmG'CkG

Cuts at:      0      90     692    1097    1128 
   Size:         90     602     405      31

  Fragments arranged by size: 

                 602     405      90      31
 
NcoI C'CATG_G

Cuts at:      0     648    1128 
   Size:        648     480
 
NspI r_CATG'y

Cuts at:      0     540    1128 
   Size:        540     588
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     265     297    1128 
   Size:        265      32     831

  Fragments arranged by size: 

                 831     265      32
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     137     169     372     404    1128 
   Size:        137      32     203      32     724

  Fragments arranged by size: 

                 724     203     137      32      32
 
PstI C_TGCA'G

Cuts at:      0     697    1128 
   Size:        697     431
 
PvuII CAG'CTG

Cuts at:      0      90     692    1097    1128 
   Size:         90     602     405      31

  Fragments arranged by size: 

                 602     405      90      31
 
SfcI C'TryA_G

Cuts at:      0     693    1128 
   Size:        693     435
 
SmlI C'TyrA_G

Cuts at:      0     541    1128 
   Size:        541     587
 
SphI G_CATG'C

Cuts at:      0     540    1128 
   Size:        540     588
 
SspI AAT'ATT

Cuts at:      0     580    1128 
   Size:        580     548
 
StyI C'CwwG_G

Cuts at:      0     648    1128 
   Size:        648     480
 
TatI w'GTAC_w

Cuts at:      0     821     937    1128 
   Size:        821     116     191

  Fragments arranged by size: 

                 821     191     116
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     318    1128 
   Size:        318     810
 
XmnI GAAnn'nnTTC

Cuts at:      0     146    1128 
   Size:        146     982
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AloI    AlwNI     ApoI     BanI
    BbvCI     BclI Bme1580I     BpmI   Bpu10I    BsaXI    BseRI    BseYI
     BsgI    BsiEI     BsmI Bsp1286I    BspHI    BsrGI    BstXI    BstYI
     BtgI     BtsI    CspCI     DraI  Eco57MI    EcoRI    HaeII    Hin4I
     MfeI     MmeI     MslI   MspA1I     NcoI     NspI     PpiI     PsrI
     PstI    PvuII     SfcI     SmlI     SphI     SspI     StyI     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BanII     BbeI     BbsI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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