Restriction map for pF1KA1739
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AgeI        AhdI        AocI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BbrPI       BclI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaI        BsaWI       BseAI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        ScaI        SfuI        SnaBI       SpeI        
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tsp509I     TthHB8I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AccI          703  GTCTAC               GT^[AC][GT]AC
AflII        2227  CTTAAG               C^TTAAG
AflIII        968  ACATGT               A^C[GA][CT]GT
Alw44I        408  GTGCAC               G^TGCAC
ApaLI         408  GTGCAC               G^TGCAC
BamHI        2193  GGATCC               G^GATCC
BbvCI          61  GCTGAGG              CCTCAGC(-5/-2)
BciVI         296  GTATCC               GTATCC(6/5)
BfaI         2380  CTAG                 C^TAG
BfrI         2227  CTTAAG               C^TTAAG
BsiEI        1275  CGACCG               CG[GA][CT]^CG
BspLU11I      968  ACATGT               A^CATGT
BsrDI        2054  CATTGC               GCAATG(2/0)
BstEII       1950  GGTGACC              G^GT[ACGT]ACC
BstPI        1950  GGTGACC              G^GT[ACGT]ACC
DraIII       1264  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          694  GACGACAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1377  CCTGATTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1950  GGTGACC              G^GT[ACGT]ACC
MaeI         2380  CTAG                 C^TAG
SacI         2422  GAGCTC               GAGCT^C
SapI         2074  GAAGAGC              GCTCTTC(1/4)
SbfI          906  CCTGCAGG             CCTGCA^GG
SfiI          496  GGCCATGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        2059  CGCCGGCG             C[GA]^CCGG[CT]G
Sse8387I      906  CCTGCAGG             CCTGCA^GG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AcyI          202  GGCGCC               G[GA]^CG[CT]C
             1127  GGCGCC              
AspHI         408  GTGCAC               G[AT]GC[AT]^C
             2422  GAGCTC              
BalI          113  TGGCCA               TGG^CCA
             1590  TGGCCA              
BbeI          202  GGCGCC               GGCGC^C
             1127  GGCGCC              
BcgI          738  CGACTGCTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1939  GCAGGCCCGTCG        
BsaAI         196  TACGTG               [CT]AC^GT[GA]
             2365  TACGTG              
BsaHI         202  GGCGCC               G[GA]^CG[CT]C
             1127  GGCGCC              
BsgI         1477  GTGCAG               GTGCAG(16/14)
             1517  GTGCAG              
BsiHKAI       408  GTGCAC               G[AT]GC[AT]^C
             2422  GAGCTC              
BsmAI        1331  GAGAC                GTCTC(1/5)
             2439  GAGAC               
BsmBI        1331  GAGACG               CGTCTC(1/5)
             2439  GAGACG              
BsrBI        1464  CCGCTC               CCGCTC(-3/-3)
              721  GAGCGG              
BstXI        1142  CCAGCTCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2256  CCAGCCCGGTGG        
EarI         1003  GAAGAG               CTCTTC(1/4)
             2074  GAAGAG              
HaeII         202  GGCGCC               [GA]GCGC^[CT]
             1127  GGCGCC              
Hin1I         202  GGCGCC               G[GA]^CG[CT]C
             1127  GGCGCC              
HincII        418  GTCAAC               GT[CT]^[GA]AC
             1561  GTCAAC              
HindII        418  GTCAAC               GT[CT]^[GA]AC
             1561  GTCAAC              
KasI          202  GGCGCC               G^GCGCC
             1127  GGCGCC              
Ksp632I      1003  GAAGAG               CTCTTC(1/4)
             2074  GAAGAG              
MluNI         113  TGGCCA               TGG^CCA
             1590  TGGCCA              
MscI          113  TGGCCA               TGG^CCA
             1590  TGGCCA              
MseI         1112  TTAA                 T^TAA
             2228  TTAA                
MslI         1692  CATCACGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1695  CACGGTGGTG          
NaeI         1456  GCCGGC               GCC^GGC
             2060  GCCGGC              
NarI          202  GGCGCC               GG^CGCC
             1127  GGCGCC              
NgoMIV       1456  GCCGGC               G^CCGGC
             2060  GCCGGC              
PflMI        1263  CCACCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1827  CCAGGCCCTGG         
SexAI        1265  ACCTGGT              A^CC[AT]GGT
             1715  ACCTGGT             
SfoI          202  GGCGCC               GGC^GCC
             1127  GGCGCC              
SmlI         1345  CTCAAG               C^T[CT][GA]AG
             2227  CTTAAG              
SphI          850  GCATGC               GCATG^C
             2244  GCATGC              
Tru9I        1112  TTAA                 T^TAA
             2228  TTAA                
Tsp45I        965  GTGAC                ^GT[GC]AC
             1951  GTGAC               
Van91I       1263  CCACCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1827  CCAGGCCCTGG         
XcmI         1412  CCATGAGAAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1568  CCAGCCGCTGTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        AspI        AvaI        AvaII       
BanI        BanII       BbsI        BbvI        BcnI        
BglI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BssSI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflFI       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        StyI        TseI        
TspRI       Tth111I     XhoII       XmaCI       XmaI        
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