Restriction map for pF1KA1592
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AflII       AgeI        Aor51HI     AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        BamHI       
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglII       BsaAI       BsaBI       BsaWI       
BseAI       BsiWI       BspDI       BspEI       BspMI       
BsrDI       BssHII      Bst1107I    BstAPI      BstUI       
BstZ17I     ClaI        CpoI        DraI        Eco47III    
EcoRI       EcoT22I     FbaI        FseI        HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MseI        MspA1I      MunI        
MvnI        NdeI        NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacII       
SalI        SexAI       SfiI        SgrAI       SnaBI       
SpeI        SphI        StuI        SwaI        Tru9I       
Tth111I     Van91I      XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AatII        1582  GACGTC               GACGT^C
AcsI          487  AAATTC               [GA]^AATT[CT]
AflIII       1106  ACATGT               A^C[GA][CT]GT
AhdI          805  GACGAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         430  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          427  CCTCAGG              CC^T[ACGT]AGG
ApoI          487  AAATTC               [GA]^AATT[CT]
AspEI         805  GACGAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         209  TGCGCA               TGC^GCA
AvrII         309  CCTAGG               C^CTAGG
BalI          983  TGGCCA               TGG^CCA
BbeI         1468  GGCGCC               GGCGC^C
BlnI          309  CCTAGG               C^CTAGG
BlpI         1550  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1550  GCTCAGC              GC^T[ACGT]AGC
BsmI         1884  GAATGC               GAATGC(1/-1)
BspLU11I     1106  ACATGT               A^CATGT
BsrFI        1389  GCCGGC               [GA]^CCGG[CT]
BstBI         799  TTCGAA               TT^CGAA
BstXI        1778  CCACCCACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        427  CCTCAGG              CC^T[ACGT]AGG
CelII        1550  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1389  GCCGGC               [GA]^CCGG[CT]
DraIII        907  CACCCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1657  GACTTCAGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          200  CCATGG               C^C[GA][CT]GG
EagI         1391  CGGCCG               C^GGCCG
Eam1105I      805  GACGAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1391  CGGCCG               C^GGCCG
Eco52I       1391  CGGCCG               C^GGCCG
Eco81I        427  CCTCAGG              CC^T[ACGT]AGG
EcoNI         423  CCTACCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI          209  TGCGCA               TGC^GCA
HgaI         1664  GCGTC                GACGC(5/10)
HincII        325  GTCAAC               GT[CT]^[GA]AC
HindII        325  GTCAAC               GT[CT]^[GA]AC
KasI         1468  GGCGCC               G^GCGCC
MluNI         983  TGGCCA               TGG^CCA
MscI          983  TGGCCA               TGG^CCA
NaeI         1389  GCCGGC               GCC^GGC
NarI         1468  GGCGCC               GG^CGCC
NcoI          200  CCATGG               C^CATGG
NgoMIV       1389  GCCGGC               G^CCGGC
NspI         1106  ACATGT               [GA]CATG^[CT]
NspV          799  TTCGAA               TT^CGAA
PpuMI        1034  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI        1500  GACCTCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI           75  GCTCTTC              GCTCTTC(1/4)
SbfI         1413  CCTGCAGG             CCTGCA^GG
ScaI         1352  AGTACT               AGT^ACT
SfoI         1468  GGCGCC               GGC^GCC
SfuI          799  TTCGAA               TT^CGAA
SmaI           23  CCCGGG               CCC^GGG
Sse8387I     1413  CCTGCAGG             CCTGCA^GG
SspI          817  AATATT               AAT^ATT
TfiI          561  GATTC                G^A[AT]TC
XbaI          348  TCTAGA               T^CTAGA
XmaCI          23  CCCGGG               C^CCGGG
XmaI           23  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AcyI         1468  GGCGCC               G[GA]^CG[CT]C
             1582  GACGTC              
ApaI           26  GGGCCC               GGGCC^C
               40  GGGCCC              
AvaI           23  CCCGGG               C^[CT]CG[GA]G
              178  CTCGGG              
BbsI          802  GAAGAC               GAAGAC(2/6)
              922  GTCTTC              
BpuAI         802  GAAGAC               GAAGAC(2/6)
              922  GTCTTC              
BsaHI        1468  GGCGCC               G[GA]^CG[CT]C
             1582  GACGTC              
BsaI         1828  GAGACC               GGTCTC(1/5)
             1251  GGTCTC              
BsiEI        1391  CGGCCG               CG[GA][CT]^CG
             1515  CGACCG              
BsmBI        1584  CGTCTC               CGTCTC(1/5)
             1462  GAGACG              
BsoBI          23  CCCGGG               C^[CT]CG[GA]G
              178  CTCGGG              
Bsp1407I     1109  TGTACA               T^GTACA
             1373  TGTACA              
BspHI         695  TCATGA               T^CATGA
              719  TCATGA              
BsrBI        1226  CCGCTC               CCGCTC(-3/-3)
             1548  CCGCTC              
BsrGI        1109  TGTACA               T^GTACA
             1373  TGTACA              
BsrI         1620  CCAGT                ACTGG(1/-1)
             1644  CCAGT               
BstEII       1018  GGTGACC              G^GT[ACGT]ACC
             1047  GGTCACC             
BstPI        1018  GGTGACC              G^GT[ACGT]ACC
             1047  GGTCACC             
EarI           76  CTCTTC               CTCTTC(1/4)
             1838  CTCTTC              
EcoO65I      1018  GGTGACC              G^GT[ACGT]ACC
             1047  GGTCACC             
EcoRV         691  GATATC               GAT^ATC
             1275  GATATC              
HaeII         245  GGCGCT               [GA]GCGC^[CT]
             1468  GGCGCC              
Hin1I        1468  GGCGCC               G[GA]^CG[CT]C
             1582  GACGTC              
Ksp632I        76  CTCTTC               CTCTTC(1/4)
             1838  CTCTTC              
PleI           67  GACTC                GAGTC(4/5)
             1099  GAGTC               
PstI         1414  CTGCAG               CTGCA^G
             1592  CTGCAG              
RcaI          695  TCATGA               T^CATGA
              719  TCATGA              
SacI          640  GAGCTC               GAGCT^C
             1189  GAGCTC              
SspBI        1109  TGTACA               T^GTACA
             1373  TGTACA              
TaqI          800  TCGA                 T^CGA
             1067  TCGA                
Tsp509I       132  AATT                 ^AATT
              488  AATT                
TthHB8I       800  TCGA                 T^CGA
             1067  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AciI        AfaI        AluI        Alw44I      AlwI        
ApaLI       AspHI       AvaII       BanI        BanII       
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BssKI       BssSI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TseI        
Tsp45I      TspRI       XhoII       
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