Restriction map for pF1KA1592
 (Linear) (Six-base) MAPSORT of: KIAA1592.seq  Check: 5653  from: 1  to: 1893



KIAA1592.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AatII G_ACGT'C

Cuts at:      0    1586    1893 
   Size:       1586     307
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70    1644    1893 
   Size:         70    1574     249

  Fragments arranged by size: 

                1574     249      70
 
AflIII A'CryG_T

Cuts at:      0    1106    1893 
   Size:       1106     787
 
AhdI GACnn_n'nnGTC

Cuts at:      0     810    1893 
   Size:        810    1083
 
AlwNI CAG_nnn'CTG

Cuts at:      0     435    1893 
   Size:        435    1458
 
ApaI G_GGCC'C

Cuts at:      0      30      44    1893 
   Size:         30      14    1849

  Fragments arranged by size: 

                1849      30      14
 
ApaLI G'TGCA_C

Cuts at:      0      18     913    1773    1893 
   Size:         18     895     860     120

  Fragments arranged by size: 

                 895     860     120      18
 
ApoI r'AATT_y

Cuts at:      0     487    1893 
   Size:        487    1406
 
AvaI C'yCGr_G

Cuts at:      0      23     178    1893 
   Size:         23     155    1715

  Fragments arranged by size: 

                1715     155      23
 
AvrII C'CTAG_G

Cuts at:      0     309    1893 
   Size:        309    1584
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1291    1324    1893 
   Size:       1291      33     569

  Fragments arranged by size: 

                1291     569      33
 
BanI G'GyrC_C

Cuts at:      0     229     463     658    1468    1893 
   Size:        229     234     195     810     425

  Fragments arranged by size: 

                 810     425     234     229     195
 
BanII G_rGCy'C

Cuts at:      0      30      44     269     617     644    1193    1893 
   Size:         30      14     225     348      27     549     700

  Fragments arranged by size: 

                 700     549     348     225      30      27      14
 
BbeI G_GCGC'C

Cuts at:      0    1472    1893 
   Size:       1472     421
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     809     915    1893 
   Size:        809     106     978

  Fragments arranged by size: 

                 978     809     106
 
BglI GCCn_nnn'nGGC

Cuts at:      0      39     114     273    1893 
   Size:         39      75     159    1620

  Fragments arranged by size: 

                1620     159      75      39
 
BlpI GC'TnA_GC

Cuts at:      0    1551    1893 
   Size:       1551     342
 
Bme1580I G_kGCm'C

Cuts at:      0      22      30      44     663     917    1674    1777    1893 
   Size:         22       8      14     619     254     757     103     116

  Fragments arranged by size: 

                 757     619     254     116     103      22      14       8
            
 
BmgBI CAC'GTC

Cuts at:      0    1062    1893 
   Size:       1062     831
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1872    1893 
   Size:       1872      21
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     691     973    1075    1893 
   Size:        691     282     102     818

  Fragments arranged by size: 

                 818     691     282     102
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     865    1288    1315    1893 
   Size:        865     423      27     578

  Fragments arranged by size: 

                 865     578     423      27
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1257    1822    1893 
   Size:       1257     565      71

  Fragments arranged by size: 

                1257     565      71
 
BsaHI Gr'CG_yC

Cuts at:      0    1469    1583    1893 
   Size:       1469     114     310

  Fragments arranged by size: 

                1469     310     114
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1858    1888    1893 
   Size:       1858      30       5

  Fragments arranged by size: 

                1858      30       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     103     378     652     970    1084    1207    1893 
   Size:        103     275     274     318     114     123     686

  Fragments arranged by size: 

                 686     318     275     274     123     114     103
 
BseYI C'CCAG_C

Cuts at:      0      29     701    1524    1893 
   Size:         29     672     823     369

  Fragments arranged by size: 

                 823     672     369      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     238     855    1012    1849    1893 
   Size:        103     135     617     157     837      44

  Fragments arranged by size: 

                 837     617     157     135     103      44
 
BsiEI CG_ry'CG

Cuts at:      0    1394    1518    1893 
   Size:       1394     124     375

  Fragments arranged by size: 

                1394     375     124
 
BsiHKAI G_wGCw'C

Cuts at:      0      22     442     644     917    1193    1346    1777    1893 
   Size:         22     420     202     273     276     153     431     116

  Fragments arranged by size: 

                 431     420     276     273     202     153     116      22
            
 
BsmI GAATG_Cn'

Cuts at:      0    1890    1893 
   Size:       1890       3
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1456    1590    1893 
   Size:       1456     134     303

  Fragments arranged by size: 

                1456     303     134
 
Bsp1286I G_dGCh'C

Cuts at:      0      22      30      44     269     442     617     644     663 
   Size:         22       8      14     225     173     175      27      19

Cuts at:    663     917    1193    1346    1674    1777    1893 
   Size:        254     276     153     328     103     116

  Fragments arranged by size: 

                 328     276     254     225     175     173     153     116
                 103      27      22      19      14       8
 
BspHI T'CATG_A

Cuts at:      0     695     719    1893 
   Size:        695      24    1174

  Fragments arranged by size: 

                1174     695      24
 
BsrBI CCG'CTC

Cuts at:      0    1228    1550    1893 
   Size:       1228     322     343

  Fragments arranged by size: 

                1228     343     322
 
BsrFI r'CCGG_y

Cuts at:      0    1389    1893 
   Size:       1389     504
 
BsrGI T'GTAC_A

Cuts at:      0    1109    1373    1893 
   Size:       1109     264     520

  Fragments arranged by size: 

                1109     520     264
 
BssSI C'ACGA_G

Cuts at:      0     628    1675    1879    1893 
   Size:        628    1047     204      14

  Fragments arranged by size: 

                1047     628     204      14
 
BstBI TT'CG_AA

Cuts at:      0     800    1893 
   Size:        800    1093
 
BstEII G'GTnAC_C

Cuts at:      0    1018    1047    1893 
   Size:       1018      29     846

  Fragments arranged by size: 

                1018     846      29
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1785    1893 
   Size:       1785     108
 
BstYI r'GATC_y

Cuts at:      0     419    1088    1286    1893 
   Size:        419     669     198     607

  Fragments arranged by size: 

                 669     607     419     198
 
Bsu36I CC'TnA_GG

Cuts at:      0     428    1893 
   Size:        428    1465
 
BtgI C'CryG_G

Cuts at:      0     200    1893 
   Size:        200    1693
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     746    1216    1893 
   Size:        746     470     677

  Fragments arranged by size: 

                 746     677     470
 
BtsI GCAGTG_nn'

Cuts at:      0      70    1893 
   Size:         70    1823
 
DraIII CAC_nnn'GTG

Cuts at:      0     912    1893 
   Size:        912     981
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1663    1893 
   Size:       1663     230
 
EaeI y'GGCC_r

Cuts at:      0     983    1223    1391    1796    1893 
   Size:        983     240     168     405      97

  Fragments arranged by size: 

                 983     405     240     168      97
 
EagI C'GGCC_G

Cuts at:      0    1391    1893 
   Size:       1391     502
 
EarI CTCTTCn'nnn_

Cuts at:      0      82    1844    1893 
   Size:         82    1762      49

  Fragments arranged by size: 

                1762      82      49
 
EcoICRI GAG'CTC

Cuts at:      0     642    1191    1893 
   Size:        642     549     702

  Fragments arranged by size: 

                 702     642     549
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     691     973    1075    1644    1893 
   Size:         70     621     282     102     569     249

  Fragments arranged by size: 

                 621     569     282     249     102      70
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     427    1893 
   Size:        427    1466
 
EcoO109I rG'GnC_Cy

Cuts at:      0      41     432    1035    1893 
   Size:         41     391     603     858

  Fragments arranged by size: 

                 858     603     391      41
 
EcoRV GAT'ATC

Cuts at:      0     693    1277    1893 
   Size:        693     584     616

  Fragments arranged by size: 

                 693     616     584
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1176    1893 
   Size:       1176     717
 
FspI TGC'GCA

Cuts at:      0     211    1893 
   Size:        211    1682
 
HaeII r_GCGC'y

Cuts at:      0     249    1472    1893 
   Size:        249    1223     421

  Fragments arranged by size: 

                1223     421     249
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      52      84     411     443     796     828     876     908 
   Size:         52      32     327      32     353      32      48      32

Cuts at:    908    1821    1853    1893 
   Size:        913      32      40

  Fragments arranged by size: 

                 913     353     327      52      48      40      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     327    1893 
   Size:        327    1566
 
KasI G'GCGC_C

Cuts at:      0    1468    1893 
   Size:       1468     425
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     740     920    1064    1127    1538    1893 
   Size:        740     180     144      63     411     355

  Fragments arranged by size: 

                 740     411     355     180     144      63
 
MscI TGG'CCA

Cuts at:      0     985    1893 
   Size:        985     908
 
MslI CAynn'nnrTG

Cuts at:      0     379     793    1783    1883    1893 
   Size:        379     414     990     100      10

  Fragments arranged by size: 

                 990     414     379     100      10
 
NaeI GCC'GGC

Cuts at:      0    1391    1893 
   Size:       1391     502
 
NarI GG'CG_CC

Cuts at:      0    1469    1893 
   Size:       1469     424
 
NcoI C'CATG_G

Cuts at:      0     200    1893 
   Size:        200    1693
 
NgoMIV G'CCGG_C

Cuts at:      0    1389    1893 
   Size:       1389     504
 
NspI r_CATG'y

Cuts at:      0    1110    1893 
   Size:       1110     783
 
PciI A'CATG_T

Cuts at:      0    1106    1893 
   Size:       1106     787
 
PfoI T'CCnGG_A

Cuts at:      0     536     707     740    1091    1893 
   Size:        536     171      33     351     802

  Fragments arranged by size: 

                 802     536     351     171      33
 
PpuMI rG'GwC_Cy

Cuts at:      0    1035    1893 
   Size:       1035     858
 
PshAI GACnn'nnGTC

Cuts at:      0    1504    1893 
   Size:       1504     389
 
PspOMI G'GGCC_C

Cuts at:      0      26      40    1893 
   Size:         26      14    1853

  Fragments arranged by size: 

                1853      26      14
 
PstI C_TGCA'G

Cuts at:      0    1418    1596    1893 
   Size:       1418     178     297

  Fragments arranged by size: 

                1418     297     178
 
SacI G_AGCT'C

Cuts at:      0     644    1193    1893 
   Size:        644     549     700

  Fragments arranged by size: 

                 700     644     549
 
SapI GCTCTTCn'nnn_

Cuts at:      0      82    1893 
   Size:         82    1811
 
SbfI CC_TGCA'GG

Cuts at:      0    1418    1893 
   Size:       1418     475
 
ScaI AGT'ACT

Cuts at:      0    1354    1893 
   Size:       1354     539
 
SfcI C'TryA_G

Cuts at:      0     683    1414    1592    1893 
   Size:        683     731     178     301

  Fragments arranged by size: 

                 731     683     301     178
 
SfoI GGC'GCC

Cuts at:      0    1470    1893 
   Size:       1470     423
 
SmaI CCC'GGG

Cuts at:      0      25    1893 
   Size:         25    1868
 
SmlI C'TyrA_G

Cuts at:      0     880    1303    1330    1893 
   Size:        880     423      27     563

  Fragments arranged by size: 

                 880     563     423      27
 
SspI AAT'ATT

Cuts at:      0     819    1893 
   Size:        819    1074
 
StyI C'CwwG_G

Cuts at:      0     200     309    1601    1893 
   Size:        200     109    1292     292

  Fragments arranged by size: 

                1292     292     200     109
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     451    1494    1795    1893 
   Size:        451    1043     301      98

  Fragments arranged by size: 

                1043     451     301      98
 
TatI w'GTAC_w

Cuts at:      0    1109    1352    1373    1646    1688    1893 
   Size:       1109     243      21     273      42     205

  Fragments arranged by size: 

                1109     273     243     205      42      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1858    1890    1893 
   Size:       1858      32       3

  Fragments arranged by size: 

                1858      32       3
 
XbaI T'CTAG_A

Cuts at:      0     348    1893 
   Size:        348    1545
 
XmaI C'CCGG_G

Cuts at:      0      23    1893 
   Size:         23    1870
 
ZraI GAC'GTC

Cuts at:      0    1584    1893 
   Size:       1584     309
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AhdI    AlwNI     ApaI    ApaLI     ApoI
     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI     BglI
     BlpI Bme1580I    BmgBI     BplI     BpmI    BpuEI     BsaI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BsrBI    BsrFI    BsrGI    BssSI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI   DraIII     DrdI
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII     KasI     MmeI     MscI
     MslI     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PfoI
    PpuMI    PshAI   PspOMI     PstI     SacI     SapI     SbfI     ScaI
     SfcI     SfoI     SmaI     SmlI     SspI     StyI    TaqII     TatI
     TstI     XbaI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AlfI     AloI     AscI     AseI    AsiSI    BamHI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BmrI     BmtI   Bpu10I    BsaAI
    BsaBI    BsaWI    BsiWI    BspEI    BspMI    BsrDI   BssHII   BstAPI
  BstZ17I     ClaI    CspCI     DraI     EciI    EcoRI     FseI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI   MspA1I     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI     PmlI
     PpiI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI  Tth111I     XcmI     XhoI     XmnI
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