Restriction map for pF1KA1568
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# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       AccIII      AcyI        AflII       AflIII      
AgeI        AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        BbeI        BbrPI       BbvCI       
BcgI        BfrI        BglI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
BstEII      BstPI       Bsu36I      CelII       ClaI        
DraI        DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoT22I     HaeII       Hin1I       HpaI        KasI        
MamI        MfeI        MluI        MroI        MunI        
NarI        NheI        NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshBI       PvuI        RcaI        
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          600  GTATAC               GT^[AC][GT]AC
AclI         1145  AACGTT               AA^CGTT
AhdI         2022  GACTTCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        286  GTGCAC               G^TGCAC
ApaLI         286  GTGCAC               G^TGCAC
Asp700I      3998  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2022  GACTTCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1515  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          470  CCCGGG               C^[CT]CG[GA]G
AvrII        4027  CCTAGG               C^CTAGG
BciVI         545  GTATCC               GTATCC(6/5)
BclI         3114  TGATCA               T^GATCA
BlnI         4027  CCTAGG               C^CTAGG
BsmI         3009  GCATTC               GAATGC(1/-1)
BsoBI         470  CCCGGG               C^[CT]CG[GA]G
BsrFI         167  GCCGGC               [GA]^CCGG[CT]
BssSI        2790  CACGAG               CACGAG(-5/-1)
Bst1107I      600  GTATAC               GTA^TAC
BstAPI       3483  GCAACCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         515  TTCGAA               TT^CGAA
BstZ17I       600  GTATAC               GTA^TAC
Cfr10I        167  GCCGGC               [GA]^CCGG[CT]
CpoI         2389  CGGTCCG              CG^G[AT]CCG
Eam1105I     2022  GACTTCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        1849  GATATC               GAT^ATC
FbaI         3114  TGATCA               T^GATCA
FseI          166  GGCCGGCC             GGCCGG^CC
HindIII      3677  AAGCTT               A^AGCTT
KspI           91  CCGCGG               CCGC^GG
NaeI          167  GCCGGC               GCC^GGC
NcoI         2764  CCATGG               C^CATGG
NgoMIV        167  GCCGGC               G^CCGGC
NspV          515  TTCGAA               TT^CGAA
PflFI        1515  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        3383  GGGTCCC              [GA]G^G[AT]CC[CT]
Psp1406I     1145  AACGTT               AA^CGTT
RsrII        2389  CGGTCCG              CG^G[AT]CCG
SacI          932  GAGCTC               GAGCT^C
SacII          91  CCGCGG               CCGC^GG
SexAI         698  ACCAGGT              A^CC[AT]GGT
SfuI          515  TTCGAA               TT^CGAA
SmaI          470  CCCGGG               CCC^GGG
SmlI          977  CTCAAG               C^T[CT][GA]AG
Tth111I      1515  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3741  TCTAGA               T^CTAGA
XmaCI         470  CCCGGG               C^CCGGG
XmaI          470  CCCGGG               C^CCGGG
XmnI         3998  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Acc65I        613  GGTACC               G^GTACC
             1633  GGTACC              
AlwNI        1517  CAGAGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1829  CAGATCCTG           
Asp718I       613  GGTACC               G^GTACC
             1633  GGTACC              
AviII         278  TGCGCA               TGC^GCA
             1838  TGCGCA              
BalI         2782  TGGCCA               TGG^CCA
             3981  TGGCCA              
BsaI         1121  GAGACC               GGTCTC(1/5)
             1804  GGTCTC              
BspMI        1559  ACCTGC               ACCTGC(4/8)
             1584  GCAGGT              
BstXI         617  CCAATATGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3714  CCAGACTCCTGG        
DraII        3383  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             3694  AGGCCCC             
EcoO109I     3383  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             3694  AGGCCCC             
FauI           90  CCCGC                CCCGC(4/6)
             1156  GCGGG               
FspI          278  TGCGCA               TGC^GCA
             1838  TGCGCA              
HincII       2098  GTCAAC               GT[CT]^[GA]AC
             3513  GTTGAC              
HindII       2098  GTCAAC               GT[CT]^[GA]AC
             3513  GTTGAC              
KpnI          613  GGTACC               GGTAC^C
             1633  GGTACC              
MluNI        2782  TGGCCA               TGG^CCA
             3981  TGGCCA              
MscI         2782  TGGCCA               TGG^CCA
             3981  TGGCCA              
NdeI         2631  CATATG               CA^TATG
             2987  CATATG              
PshAI        1813  GACCCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3437  GACAGCAGTC          
StuI         3850  AGGCCT               AGG^CCT
             3938  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AspHI       AvaII       BamHI       
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BglII       BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EaeI        EarI        
EcoNI       EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
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