Restriction map for pF1KA1568
 (Linear) (Six-base) MAPSORT of: KIAA1568.seq  Check: 5298  from: 1  to: 4134



KIAA1568.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AccI GT'mk_AC

Cuts at:      0     601    4134 
   Size:        601    3533
 
Acc65I G'GTAC_C

Cuts at:      0     613    1633    4134 
   Size:        613    1020    2501

  Fragments arranged by size: 

                2501    1020     613
 
AclI AA'CG_TT

Cuts at:      0    1146    4134 
   Size:       1146    2988
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2009    2216    4134 
   Size:       2009     207    1918

  Fragments arranged by size: 

                2009    1918     207
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2027    4134 
   Size:       2027    2107
 
AleI CACnn'nnGTG

Cuts at:      0    2560    4134 
   Size:       2560    1574
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3464    4134 
   Size:       3464     670
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1522    1834    4134 
   Size:       1522     312    2300

  Fragments arranged by size: 

                2300    1522     312
 
ApaLI G'TGCA_C

Cuts at:      0     286    4134 
   Size:        286    3848
 
ApoI r'AATT_y

Cuts at:      0     727    1428    2055    2838    4134 
   Size:        727     701     627     783    1296

  Fragments arranged by size: 

                1296     783     727     701     627
 
AvaI C'yCGr_G

Cuts at:      0     470    4134 
   Size:        470    3664
 
AvrII C'CTAG_G

Cuts at:      0    4027    4134 
   Size:       4027     107
 
BamHI G'GATC_C

Cuts at:      0     262    2280    3196    3724    4015    4134 
   Size:        262    2018     916     528     291     119

  Fragments arranged by size: 

                2018     916     528     291     262     119
 
BanI G'GyrC_C

Cuts at:      0     613     865    1633    2599    3311    3945    4134 
   Size:        613     252     768     966     712     634     189

  Fragments arranged by size: 

                 966     768     712     634     613     252     189
 
BanII G_rGCy'C

Cuts at:      0     140     936    4102    4134 
   Size:        140     796    3166      32

  Fragments arranged by size: 

                3166     796     140      32
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      61    1912    2728    4134 
   Size:         61    1851     816    1406

  Fragments arranged by size: 

                1851    1406     816      61
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     556    4134 
   Size:        556    3578
 
BclI T'GATC_A

Cuts at:      0    3114    4134 
   Size:       3114    1020
 
BglII A'GATC_T

Cuts at:      0    2327    3002    3084    4134 
   Size:       2327     675      82    1050

  Fragments arranged by size: 

                2327    1050     675      82
 
Bme1580I G_kGCm'C

Cuts at:      0     290    2214    3316    3950    4134 
   Size:        290    1924    1102     634     184

  Fragments arranged by size: 

                1924    1102     634     290     184
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1980    3226    3956    4134 
   Size:       1980    1246     730     178

  Fragments arranged by size: 

                1980    1246     730     178
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     475     730    1139    1237    1515    1544    2276 
   Size:        458      17     255     409      98     278      29     732

Cuts at:   2276    3572    3635    3959    4134 
   Size:       1296      63     324     175

  Fragments arranged by size: 

                1296     732     458     409     324     278     255     175
                  98      63      29      17
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     962    4134 
   Size:        962    3172
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1115    1810    4134 
   Size:       1115     695    2324

  Fragments arranged by size: 

                2324    1115     695
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3444    3474    4134 
   Size:       3444      30     660

  Fragments arranged by size: 

                3444     660      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2276    2279    2711    3765    3949    4134 
   Size:       2276       3     432    1054     184     185

  Fragments arranged by size: 

                2276    1054     432     185     184       3
 
BseYI C'CCAG_C

Cuts at:      0     256     645    1082    1269    2606    2755    4134 
   Size:        256     389     437     187    1337     149    1379

  Fragments arranged by size: 

                1379    1337     437     389     256     187     149
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1520    1909    2477    3848    4134 
   Size:       1520     389     568    1371     286

  Fragments arranged by size: 

                1520    1371     568     389     286
 
BsiHKAI G_wGCw'C

Cuts at:      0     290     936    3708    4134 
   Size:        290     646    2772     426

  Fragments arranged by size: 

                2772     646     426     290
 
BsmI GAATG_Cn'

Cuts at:      0    3009    4134 
   Size:       3009    1125
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     129     377    2496    4134 
   Size:        129     248    2119    1638

  Fragments arranged by size: 

                2119    1638     248     129
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     290     936    2214    3316    3708    3950    4102 
   Size:        140     150     646    1278    1102     392     242     152

Cuts at:   4102    4134 
   Size:         32

  Fragments arranged by size: 

                1278    1102     646     392     242     152     150     140
                  32
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1568    1575    4134 
   Size:       1568       7    2559

  Fragments arranged by size: 

                2559    1568       7
 
BsrDI GCAATG_nn'

Cuts at:      0    2514    2724    3319    3901    4071    4134 
   Size:       2514     210     595     582     170      63

  Fragments arranged by size: 

                2514     595     582     210     170      63
 
BsrFI r'CCGG_y

Cuts at:      0     167    4134 
   Size:        167    3967
 
BssSI C'ACGA_G

Cuts at:      0    2790    4134 
   Size:       2790    1344
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3489    4134 
   Size:       3489     645
 
BstBI TT'CG_AA

Cuts at:      0     516    4134 
   Size:        516    3618
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     624    3721    4134 
   Size:        624    3097     413

  Fragments arranged by size: 

                3097     624     413
 
BstYI r'GATC_y

Cuts at:      0     262     750    1383    1830    2280    2327    3002    3084 
   Size:        262     488     633     447     450      47     675      82

Cuts at:   3084    3196    3724    4015    4134 
   Size:        112     528     291     119

  Fragments arranged by size: 

                 675     633     528     488     450     447     291     262
                 119     112      82      47
 
BstZ17I GTA'TAC

Cuts at:      0     602    4134 
   Size:        602    3532
 
BtgI C'CryG_G

Cuts at:      0      91     549    1709    1949    2764    2847    3469    4134 
   Size:         91     458    1160     240     815      83     622     665

  Fragments arranged by size: 

                1160     815     665     622     458     240      91      83
            
 
BtsI GCAGTG_nn'

Cuts at:      0     362    1295    1414    1508    3278    3446    4134 
   Size:        362     933     119      94    1770     168     688

  Fragments arranged by size: 

                1770     933     688     362     168     119      94
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     405     440    4134 
   Size:        405      35    3694

  Fragments arranged by size: 

                3694     405      35
 
EaeI y'GGCC_r

Cuts at:      0     169     955    2137    2782    2844    3981    4134 
   Size:        169     786    1182     645      62    1137     153

  Fragments arranged by size: 

                1182    1137     786     645     169     153      62
 
EarI CTCTTCn'nnn_

Cuts at:      0    1340    2650    3177    3470    3665    4134 
   Size:       1340    1310     527     293     195     469

  Fragments arranged by size: 

                1340    1310     527     469     293     195
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     175    3897    4134 
   Size:        175    3722     237

  Fragments arranged by size: 

                3722     237     175
 
EcoICRI GAG'CTC

Cuts at:      0     934    4134 
   Size:        934    3200
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     475     730    1139    1237    1515    1544    2009 
   Size:        458      17     255     409      98     278      29     465

Cuts at:   2009    2216    2276    3572    3635    3959    4134 
   Size:        207      60    1296      63     324     175

  Fragments arranged by size: 

                1296     465     458     409     324     278     255     207
                 175      98      63      60      29      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     311    2107    3401    4134 
   Size:        311    1796    1294     733

  Fragments arranged by size: 

                1796    1294     733     311
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3384    3695    4134 
   Size:       3384     311     439

  Fragments arranged by size: 

                3384     439     311
 
EcoRV GAT'ATC

Cuts at:      0    1851    4134 
   Size:       1851    2283
 
FseI GG_CCGG'CC

Cuts at:      0     171    4134 
   Size:        171    3963
 
FspI TGC'GCA

Cuts at:      0     280    1840    4134 
   Size:        280    1560    2294

  Fragments arranged by size: 

                2294    1560     280
 
FspAI rTGC'GCAy

Cuts at:      0    1840    4134 
   Size:       1840    2294
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     220     252     327     359     960     992    1517    1549 
   Size:        220      32      75      32     601      32     525      32

Cuts at:   1549    1959    1991    2001    2013    2033    2045    2158    2190 
   Size:        410      32      10      12      20      12     113      32

Cuts at:   2190    2319    2345    2351    2377    3828    3860    4134 
   Size:        129      26       6      26    1451      32     274

  Fragments arranged by size: 

                1451     601     525     410     274     220     129     113
                  75      32      32      32      32      32      32      32
                  26      26      20      12      12      10       6
 
HincII GTy'rAC

Cuts at:      0    2100    3515    4134 
   Size:       2100    1415     619

  Fragments arranged by size: 

                2100    1415     619
 
HindIII A'AGCT_T

Cuts at:      0    3677    4134 
   Size:       3677     457
 
KpnI G_GTAC'C

Cuts at:      0     617    1637    4134 
   Size:        617    1020    2497

  Fragments arranged by size: 

                2497    1020     617
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     429     876    1175    2223    2445    3038    4127    4134 
   Size:        429     447     299    1048     222     593    1089       7

  Fragments arranged by size: 

                1089    1048     593     447     429     299     222       7
            
 
MscI TGG'CCA

Cuts at:      0    2784    3983    4134 
   Size:       2784    1199     151

  Fragments arranged by size: 

                2784    1199     151
 
MslI CAynn'nnrTG

Cuts at:      0     871    2046    2303    2560    3506    4134 
   Size:        871    1175     257     257     946     628

  Fragments arranged by size: 

                1175     946     871     628     257     257
 
MspA1I CmG'CkG

Cuts at:      0      93     260     436    1030    1700    4134 
   Size:         93     167     176     594     670    2434

  Fragments arranged by size: 

                2434     670     594     176     167      93
 
NaeI GCC'GGC

Cuts at:      0     169    4134 
   Size:        169    3965
 
NcoI C'CATG_G

Cuts at:      0    2764    4134 
   Size:       2764    1370
 
NdeI CA'TA_TG

Cuts at:      0    2632    2988    4134 
   Size:       2632     356    1146

  Fragments arranged by size: 

                2632    1146     356
 
NgoMIV G'CCGG_C

Cuts at:      0     167    4134 
   Size:        167    3967
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     188     497    1284    2163    3351    4134 
   Size:        188     309     787     879    1188     783

  Fragments arranged by size: 

                1188     879     787     783     309     188
 
PfoI T'CCnGG_A

Cuts at:      0     452    1492    3720    4134 
   Size:        452    1040    2228     414

  Fragments arranged by size: 

                2228    1040     452     414
 
PpuMI rG'GwC_Cy

Cuts at:      0    3384    4134 
   Size:       3384     750
 
PshAI GACnn'nnGTC

Cuts at:      0    1817    3441    4134 
   Size:       1817    1624     693

  Fragments arranged by size: 

                1817    1624     693
 
PstI C_TGCA'G

Cuts at:      0    1421    2036    3497    4134 
   Size:       1421     615    1461     637

  Fragments arranged by size: 

                1461    1421     637     615
 
PvuII CAG'CTG

Cuts at:      0     436    1030    1700    4134 
   Size:        436     594     670    2434

  Fragments arranged by size: 

                2434     670     594     436
 
RsrII CG'GwC_CG

Cuts at:      0    2390    4134 
   Size:       2390    1744
 
SacI G_AGCT'C

Cuts at:      0     936    4134 
   Size:        936    3198
 
SacII CC_GC'GG

Cuts at:      0      94    4134 
   Size:         94    4040
 
SanDI GG'GwC_CC

Cuts at:      0    3384    4134 
   Size:       3384     750
 
SexAI A'CCwGG_T

Cuts at:      0     698    4134 
   Size:        698    3436
 
SfcI C'TryA_G

Cuts at:      0     722    1417    2032    3493    3951    4134 
   Size:        722     695     615    1461     458     183

  Fragments arranged by size: 

                1461     722     695     615     458     183
 
SmaI CCC'GGG

Cuts at:      0     472    4134 
   Size:        472    3662
 
SmlI C'TyrA_G

Cuts at:      0     977    4134 
   Size:        977    3157
 
StuI AGG'CCT

Cuts at:      0    3852    3940    4134 
   Size:       3852      88     194

  Fragments arranged by size: 

                3852     194      88
 
StyI C'CwwG_G

Cuts at:      0     744    1800    1995    2157    2764    2877    3345    3941 
   Size:        744    1056     195     162     607     113     468     596

Cuts at:   3941    4027    4134 
   Size:         86     107

  Fragments arranged by size: 

                1056     744     607     596     468     195     162     113
                 107      86
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     494    2378    4134 
   Size:        494    1884    1756

  Fragments arranged by size: 

                1884    1756     494
 
TatI w'GTAC_w

Cuts at:      0     853    2232    3327    4134 
   Size:        853    1379    1095     807

  Fragments arranged by size: 

                1379    1095     853     807
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     911     943    1314    1346    4134 
   Size:        911      32     371      32    2788

  Fragments arranged by size: 

                2788     911     371      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1518    4134 
   Size:       1518    2616
 
XbaI T'CTAG_A

Cuts at:      0    3741    4134 
   Size:       3741     393
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     767    1034    1281    3244    4134 
   Size:        767     267     247    1963     890

  Fragments arranged by size: 

                1963     890     767     267     247
 
XmaI C'CCGG_G

Cuts at:      0     470    4134 
   Size:        470    3664
 
XmnI GAAnn'nnTTC

Cuts at:      0    4002    4134 
   Size:       4002     132
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AhdI     AleI     AlfI    AlwNI
    ApaLI     ApoI     AvaI    AvrII    BamHI     BanI    BanII     BbsI
    BciVI     BclI    BglII Bme1580I     BmrI     BpmI    BpuEI     BsaI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I     BtgI     BtsI    CspCI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV    PflMI     PfoI    PpuMI    PshAI     PstI
    PvuII    RsrII     SacI    SacII    SanDI    SexAI     SfcI     SmaI
     SmlI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII   AflIII     AgeI     AloI     ApaI
     AscI     AseI    AsiSI     BaeI     BbeI    BbvCI     BcgI    BfrBI
     BglI     BlpI    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsaWI    BsiEI    BsiWI    BspEI    BspHI    BsrBI    BsrGI
   BssHII   BstEII   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI
     EagI    EcoRI     FalI    HaeII     HpaI     KasI     MfeI     MluI
     NarI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI
     PvuI     SalI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XhoI
     ZraI
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