Restriction map for
pF1KA1549
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# NO CUT ENZYMES ( 58 enzymes) #
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AatII AflII Alw44I AocI ApaLI
AscI AseI AsnI AspI AviII
AvrII BbeI BfrI BlnI Bsp1407I
BspDI BspLU11I BsrGI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI Eco81I
EcoT22I FseI FspI HpaI KasI
MfeI MunI NarI NotI NruI
NsiI NspI NspV PacI PflFI
PmeI PshBI PvuI RsrII SbfI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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Acc65I 5215 GGTACC G^GTACC
AccIII 3645 TCCGGA T^CCGGA
AgeI 2434 ACCGGT A^CCGGT
Aor51HI 34 AGCGCT AGC^GCT
Asp718I 5215 GGTACC G^GTACC
BglII 3047 AGATCT A^GATCT
BseAI 3645 TCCGGA T^CCGGA
BsiWI 5336 CGTACG C^GTACG
BspEI 3645 TCCGGA T^CCGGA
BspHI 3885 TCATGA T^CATGA
BsrBI 4113 GAGCGG CCGCTC(-3/-3)
BssHII 4383 GCGCGC G^CGCGC
DraI 1881 TTTAAA TTT^AAA
DrdI 4021 GACTCTACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1347 CGGCCG C^GGCCG
EclXI 1347 CGGCCG C^GGCCG
Eco47III 34 AGCGCT AGC^GCT
Eco52I 1347 CGGCCG C^GGCCG
EcoRI 3596 GAATTC G^AATTC
EcoRV 2614 GATATC GAT^ATC
KpnI 5215 GGTACC GGTAC^C
KspI 4395 CCGCGG CCGC^GG
MluI 2294 ACGCGT A^CGCGT
MroI 3645 TCCGGA T^CCGGA
NheI 4204 GCTAGC G^CTAGC
PaeR7I 1951 CTCGAG C^TCGAG
PinAI 2434 ACCGGT A^CCGGT
PshAI 2142 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3885 TCATGA T^CATGA
SacII 4395 CCGCGG CCGC^GG
SfiI 5321 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2433 CACCGGTG C[GA]^CCGG[CT]G
XhoI 1951 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AclI 1592 AACGTT AA^CGTT
4559 AACGTT
AhdI 1235 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4423 GACGACCTGTC
AspEI 1235 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4423 GACGACCTGTC
BamHI 2311 GGATCC G^GATCC
4704 GGATCC
BbrPI 2233 CACGTG CAC^GTG
4174 CACGTG
BcgI 4848 CGACGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1438 GCATTTTTCTCG
BclI 2285 TGATCA T^GATCA
2639 TGATCA
BsaI 1523 GAGACC GGTCTC(1/5)
690 GGTCTC
BsiEI 1347 CGGCCG CG[GA][CT]^CG
4287 CGACCG
BsmI 829 GCATTC GAATGC(1/-1)
1460 GCATTC
BstAPI 820 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2065 GCAGTCACTGC
BstEII 3589 GGTTACC G^GT[ACGT]ACC
5449 GGTTACC
BstPI 3589 GGTTACC G^GT[ACGT]ACC
5449 GGTTACC
Eam1105I 1235 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4423 GACGACCTGTC
EcoO65I 3589 GGTTACC G^GT[ACGT]ACC
5449 GGTTACC
FbaI 2285 TGATCA T^GATCA
2639 TGATCA
HindIII 1932 AAGCTT A^AGCTT
2833 AAGCTT
NaeI 1362 GCCGGC GCC^GGC
4208 GCCGGC
NdeI 434 CATATG CA^TATG
2603 CATATG
NgoMIV 1362 GCCGGC G^CCGGC
4208 GCCGGC
PmaCI 2233 CACGTG CAC^GTG
4174 CACGTG
PmlI 2233 CACGTG CAC^GTG
4174 CACGTG
Psp1406I 1592 AACGTT AA^CGTT
4559 AACGTT
PvuII 1816 CAGCTG CAG^CTG
3364 CAGCTG
SalI 2569 GTCGAC G^TCGAC
3622 GTCGAC
SapI 2660 GAAGAGC GCTCTTC(1/4)
4542 GAAGAGC
ScaI 2700 AGTACT AGT^ACT
2916 AGTACT
SexAI 4586 ACCAGGT A^CC[AT]GGT
5067 ACCAGGT
StuI 2982 AGGCCT AGG^CCT
5132 AGGCCT
XbaI 1255 TCTAGA T^CTAGA
2223 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI