Restriction map for pF1KA1522
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       AclI        AcsI        AflII       AflIII      
AhdI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       BamHI       BbrPI       
BcgI        BclI        BfrI        BglII       BsaBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        DraI        
DraIII      Eam1105I    Eco47III    EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KspI        
MamI        MfeI        MluI        MseI        MunI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        SacII       SalI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        SspBI       SspI        
SwaI        Tru9I       Tsp509I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          751  GTCTAC               GT^[AC][GT]AC
AccII         405  CGCG                 CG^CG
AccIII       2864  TCCGGA               T^CCGGA
AgeI         2433  ACCGGT               A^CCGGT
Alw44I        602  GTGCAC               G^TGCAC
ApaLI         602  GTGCAC               G^TGCAC
AviII         977  TGCGCA               TGC^GCA
AvrII         960  CCTAGG               C^CTAGG
BbeI         1559  GGCGCC               GGCGC^C
BlnI          960  CCTAGG               C^CTAGG
BsaAI         376  TACGTG               [CT]AC^GT[GA]
BsaI         2824  GAGACC               GGTCTC(1/5)
BseAI        2864  TCCGGA               T^CCGGA
BsiWI         615  CGTACG               C^GTACG
BsmI         1286  GCATTC               GAATGC(1/-1)
BspEI        2864  TCCGGA               T^CCGGA
BspMI        1651  GCAGGT               ACCTGC(4/8)
BstUI         405  CGCG                 CG^CG
BstXI        1640  CCAAGGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        2151  AGATCC               [GA]^GATC[CT]
DrdI         1822  GACAGGTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI          977  TGCGCA               TGC^GCA
KasI         1559  GGCGCC               G^GCGCC
MflI         2151  AGATCC               [GA]^GATC[CT]
MroI         2864  TCCGGA               T^CCGGA
MvnI          405  CGCG                 CG^CG
NaeI           84  GCCGGC               GCC^GGC
NarI         1559  GGCGCC               GG^CGCC
NgoMIV         84  GCCGGC               G^CCGGC
NheI         1195  GCTAGC               G^CTAGC
PflMI        1520  CCAACAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2433  ACCGGT               A^CCGGT
PshAI        1957  GACGCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI          211  GAAGAGC              GCTCTTC(1/4)
SfoI         1559  GGCGCC               GGC^GCC
Van91I       1520  CCAACAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        2151  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwI          429  GATCC                GGATC(4/5)
             2152  GATCC               
AspI          172  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              745  GACCGTGTC           
BbsI          340  GAAGAC               GAAGAC(2/6)
              178  GTCTTC              
BciVI        1603  GGATAC               GTATCC(6/5)
              311  GTATCC              
BpuAI         340  GAAGAC               GAAGAC(2/6)
              178  GTCTTC              
BsaWI        2433  ACCGGT               [AT]^CCGG[AT]
             2864  TCCGGA              
CpoI         2654  CGGTCCG              CG^G[AT]CCG
             2715  CGGACCG             
DpnII         429  GATC                 ^GATC
             2152  GATC                
EagI           82  CGGCCG               C^GGCCG
              636  CGGCCG              
EclXI          82  CGGCCG               C^GGCCG
              636  CGGCCG              
Eco52I         82  CGGCCG               C^GGCCG
              636  CGGCCG              
MboI          429  GATC                 ^GATC
             2152  GATC                
MslI          118  CATCTAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1125  CACCATTGTG          
NcoI          294  CCATGG               C^CATGG
             2204  CCATGG              
NdeII         429  GATC                 ^GATC
             2152  GATC                
PaeR7I       1608  CTCGAG               C^TCGAG
             2981  CTCGAG              
PflFI         172  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              745  GACCGTGTC           
PpuMI        1653  AGGTCCC              [GA]G^G[AT]CC[CT]
             2195  AGGACCT             
RsrII        2654  CGGTCCG              CG^G[AT]CCG
             2715  CGGACCG             
SacI          387  GAGCTC               GAGCT^C
             1185  GAGCTC              
Sau3AI        429  GATC                 ^GATC
             2152  GATC                
SbfI          678  CCTGCAGG             CCTGCA^GG
             2260  CCTGCAGG            
Sse8387I      678  CCTGCAGG             CCTGCA^GG
             2260  CCTGCAGG            
Tth111I       172  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              745  GACCGTGTC           
XhoI         1608  CTCGAG               C^TCGAG
             2981  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

Acc65I      AciI        AcyI        AfaI        AluI        
AlwNI       AocI        ApaI        Asp718I     AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvCI       BbvI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BsaHI       
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DraII       DsaI        EaeI        EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KpnI        Ksp632I     
MaeI        MaeII       MaeIII      MboII       MluNI       
MnlI        MscI        MspA1I      MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RsaI        Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
StuI        StyI        TaqI        TfiI        TseI        
Tsp45I      TspRI       TthHB8I     XcmI        XmaCI       
XmaI        
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