Restriction map for pF1KA1451
 (Linear) (Six-base) MAPSORT of: KIAA1451.seq  Check: 9566  from: 1  to: 2667



KIAA1451.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1159    2667 
   Size:       1159    1508
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     728    1220    1459    2120    2667 
   Size:        728     492     239     661     547

  Fragments arranged by size: 

                 728     661     547     492     239
 
AflII C'TTAA_G

Cuts at:      0     361    2667 
   Size:        361    2306
 
AflIII A'CryG_T

Cuts at:      0     870    1780    2667 
   Size:        870     910     887

  Fragments arranged by size: 

                 910     887     870
 
AleI CACnn'nnGTG

Cuts at:      0     871     914    2667 
   Size:        871      43    1753

  Fragments arranged by size: 

                1753     871      43
 
AlwNI CAG_nnn'CTG

Cuts at:      0     111    1192    2404    2667 
   Size:        111    1081    1212     263

  Fragments arranged by size: 

                1212    1081     263     111
 
ApoI r'AATT_y

Cuts at:      0    1730    1813    2020    2667 
   Size:       1730      83     207     647

  Fragments arranged by size: 

                1730     647     207      83
 
AseI AT'TA_AT

Cuts at:      0    2002    2667 
   Size:       2002     665
 
AvaI C'yCGr_G

Cuts at:      0    2072    2667 
   Size:       2072     595
 
AvrII C'CTAG_G

Cuts at:      0    1833    2667 
   Size:       1833     834
 
BamHI G'GATC_C

Cuts at:      0     694    2667 
   Size:        694    1973
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     796    2667 
   Size:        796    1871
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1615    1789    2667 
   Size:       1615     174     878

  Fragments arranged by size: 

                1615     878     174
 
BclI T'GATC_A

Cuts at:      0     728     821    1005    2667 
   Size:        728      93     184    1662

  Fragments arranged by size: 

                1662     728     184      93
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2361    2667 
   Size:       2361     306
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1888    2488    2667 
   Size:       1888     600     179

  Fragments arranged by size: 

                1888     600     179
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1774    2667 
   Size:       1774     893
 
Bpu10I CC'TnA_GC

Cuts at:      0     847    1103    1594    2667 
   Size:        847     256     491    1073

  Fragments arranged by size: 

                1073     847     491     256
 
BsaAI yAC'GTr

Cuts at:      0     895    2667 
   Size:        895    1772
 
BsaBI GATnn'nnATC

Cuts at:      0     176    2667 
   Size:        176    2491
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2409    2667 
   Size:       2409     258
 
BsmI GAATG_Cn'

Cuts at:      0     585    2667 
   Size:        585    2082
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1159    2667 
   Size:       1159    1508
 
BsrDI GCAATG_nn'

Cuts at:      0    1999    2667 
   Size:       1999     668
 
BsrFI r'CCGG_y

Cuts at:      0    2577    2667 
   Size:       2577      90
 
BstBI TT'CG_AA

Cuts at:      0    2553    2667 
   Size:       2553     114
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     550    2667 
   Size:        550    2117
 
BstYI r'GATC_y

Cuts at:      0     432     694    2667 
   Size:        432     262    1973

  Fragments arranged by size: 

                1973     432     262
 
BtgI C'CryG_G

Cuts at:      0    2248    2582    2667 
   Size:       2248     334      85

  Fragments arranged by size: 

                2248     334      85
 
BtsI GCAGTG_nn'

Cuts at:      0     671    2667 
   Size:        671    1996
 
DraI TTT'AAA

Cuts at:      0    1121    1818    2667 
   Size:       1121     697     849

  Fragments arranged by size: 

                1121     849     697
 
EaeI y'GGCC_r

Cuts at:      0    2579    2667 
   Size:       2579      88
 
EarI CTCTTCn'nnn_

Cuts at:      0    2168    2555    2667 
   Size:       2168     387     112

  Fragments arranged by size: 

                2168     387     112
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     728    1220    1459    1774    2120    2667 
   Size:        728     492     239     315     346     547

  Fragments arranged by size: 

                 728     547     492     346     315     239
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1261    1348    2667 
   Size:       1261      87    1319

  Fragments arranged by size: 

                1319    1261      87
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2253    2667 
   Size:       2253     414
 
EcoRI G'AATT_C

Cuts at:      0    1730    2667 
   Size:       1730     937
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      82     376     636    1750    2667 
   Size:         82     294     260    1114     917

  Fragments arranged by size: 

                1114     917     294     260      82
 
HaeII r_GCGC'y

Cuts at:      0     138    2667 
   Size:        138    2529
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1330    1362    2029    2061    2667 
   Size:       1330      32     667      32     606

  Fragments arranged by size: 

                1330     667     606      32      32
 
HincII GTy'rAC

Cuts at:      0    1670    2667 
   Size:       1670     997
 
HindIII A'AGCT_T

Cuts at:      0     149    2667 
   Size:        149    2518
 
HpaI GTT'AAC

Cuts at:      0    1670    2667 
   Size:       1670     997
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     672    1999    2667 
   Size:        181     491    1327     668

  Fragments arranged by size: 

                1327     668     491     181
 
MslI CAynn'nnrTG

Cuts at:      0     871     914    2667 
   Size:        871      43    1753

  Fragments arranged by size: 

                1753     871      43
 
NcoI C'CATG_G

Cuts at:      0    2248    2667 
   Size:       2248     419
 
NspI r_CATG'y

Cuts at:      0     874    1784    2667 
   Size:        874     910     883

  Fragments arranged by size: 

                 910     883     874
 
PciI A'CATG_T

Cuts at:      0     870    1780    2667 
   Size:        870     910     887

  Fragments arranged by size: 

                 910     887     870
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     500     645    2667 
   Size:        500     145    2022

  Fragments arranged by size: 

                2022     500     145
 
PfoI T'CCnGG_A

Cuts at:      0    1304    2667 
   Size:       1304    1363
 
PpuMI rG'GwC_Cy

Cuts at:      0    2253    2667 
   Size:       2253     414
 
PsiI TTA'TAA

Cuts at:      0     305    1451    2667 
   Size:        305    1146    1216

  Fragments arranged by size: 

                1216    1146     305
 
PspXI vC'TCGA_Gb

Cuts at:      0    2072    2667 
   Size:       2072     595
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     554     586    2667 
   Size:        554      32    2081

  Fragments arranged by size: 

                2081     554      32
 
SanDI GG'GwC_CC

Cuts at:      0    2253    2667 
   Size:       2253     414
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2555    2667 
   Size:       2555     112
 
SmlI C'TyrA_G

Cuts at:      0     361    2072    2667 
   Size:        361    1711     595

  Fragments arranged by size: 

                1711     595     361
 
SspI AAT'ATT

Cuts at:      0    1649    1776    2541    2667 
   Size:       1649     127     765     126

  Fragments arranged by size: 

                1649     765     127     126
 
StyI C'CwwG_G

Cuts at:      0      66     138    1262    1833    2248    2667 
   Size:         66      72    1124     571     415     419

  Fragments arranged by size: 

                1124     571     419     415      72      66
 
TatI w'GTAC_w

Cuts at:      0     421    2452    2667 
   Size:        421    2031     215

  Fragments arranged by size: 

                2031     421     215
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1156    2667 
   Size:       1156    1511
 
XhoI C'TCGA_G

Cuts at:      0    2072    2667 
   Size:       2072     595
 

 Enzymes that do cut:

     AarI     AcuI    AflII   AflIII     AleI    AlwNI     ApoI     AseI
     AvaI    AvrII    BamHI     BbsI    BciVI     BclI     BglI     BmrI
     BpmI   Bpu10I    BsaAI    BsaBI    BseRI     BsmI    BspMI    BsrDI
    BsrFI    BstBI    BstXI    BstYI     BtgI     BtsI     DraI     EaeI
     EarI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I
   HincII  HindIII     HpaI     MmeI     MslI     NcoI     NspI     PciI
    PflMI     PfoI    PpuMI     PsiI    PspXI     PsrI    SanDI     SapI
     SmlI     SspI     StyI     TatI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI     AlfI
     AloI     ApaI    ApaLI     AscI    AsiSI     BaeI     BanI    BanII
     BbeI    BbvCI     BcgI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI   BstEII  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoRV     FseI     FspI    FspAI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PshAI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XmaI     XmnI     ZraI
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