Restriction map for pF1KA1384
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        AocI        
Aor51HI     AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BsaBI       BseAI       BsiWI       BsmBI       BspDI       
BspEI       BspLU11I    BssSI       Bst1107I    BstBI       
BstZ17I     Bsu36I      CelII       ClaI        DdeI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoRV       
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MluI        MroI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    SacI        
SacII       SalI        SbfI        SfoI        SfuI        
SgrAI       SpeI        Sse8387I    SspI        StuI        
SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcyI          886  GACGCC               G[GA]^CG[CT]C
Alw44I       1402  GTGCAC               G^TGCAC
ApaI          236  GGGCCC               GGGCC^C
ApaLI        1402  GTGCAC               G^TGCAC
Asp700I      1584  GAAAGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI         1047  GAAGAC               GAAGAC(2/6)
BfaI         1336  CTAG                 C^TAG
BglII         539  AGATCT               A^GATCT
BpuAI        1047  GAAGAC               GAAGAC(2/6)
BsaAI        1279  TACGTA               [CT]AC^GT[GA]
BsaHI         886  GACGCC               G[GA]^CG[CT]C
BsaWI         999  ACCGGA               [AT]^CCGG[AT]
BsmI         1618  GAATGC               GAATGC(1/-1)
Bsp1407I     1434  TGTACA               T^GTACA
BspHI         779  TCATGA               T^CATGA
BsrBI         826  GAGCGG               CCGCTC(-3/-3)
BsrDI         133  CATTGC               GCAATG(2/0)
BsrFI        1004  ACCGGC               [GA]^CCGG[CT]
BsrGI        1434  TGTACA               T^GTACA
BssHII        663  GCGCGC               G^CGCGC
BstAPI       1831  GCAGGCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I       1004  ACCGGC               [GA]^CCGG[CT]
CpoI         1001  CGGACCG              CG^G[AT]CCG
EcoNI         624  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         947  GAATTC               G^AATTC
EcoT22I      1253  ATGCAT               ATGCA^T
HgaI          886  GACGC                GACGC(5/10)
Hin1I         886  GACGCC               G[GA]^CG[CT]C
MaeI         1336  CTAG                 C^TAG
MfeI         1565  CAATTG               C^AATTG
MseI         1202  TTAA                 T^TAA
MunI         1565  CAATTG               C^AATTG
NcoI         1456  CCATGG               C^CATGG
NsiI         1253  ATGCAT               ATGCA^T
PaeR7I        415  CTCGAG               C^TCGAG
PflMI         834  CCAGTCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          19  AGGACCT              [GA]G^G[AT]CC[CT]
PvuI          175  CGATCG               CGAT^CG
RcaI          779  TCATGA               T^CATGA
RsrII        1001  CGGACCG              CG^G[AT]CCG
ScaI          167  AGTACT               AGT^ACT
SmlI          415  CTCGAG               C^T[CT][GA]AG
SnaBI        1279  TACGTA               TAC^GTA
SphI          758  GCATGC               GCATG^C
SspBI        1434  TGTACA               T^GTACA
Tru9I        1202  TTAA                 T^TAA
Van91I        834  CCAGTCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          415  CTCGAG               C^TCGAG
XmnI         1584  GAAAGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcsI          947  GAATTC               [GA]^AATT[CT]
             1173  AAATTT              
ApoI          947  GAATTC               [GA]^AATT[CT]
             1173  AAATTT              
AspI          441  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              859  GACCCGGTC           
BalI          611  TGGCCA               TGG^CCA
              938  TGGCCA              
BsaI          598  GAGACC               GGTCTC(1/5)
              751  GAGACC              
BsiEI         175  CGATCG               CG[GA][CT]^CG
              744  CGACCG              
BsmAI         598  GAGAC                GTCTC(1/5)
              751  GAGAC               
BstEII        504  GGTCACC              G^GT[ACGT]ACC
             1636  GGTGACC             
BstPI         504  GGTCACC              G^GT[ACGT]ACC
             1636  GGTGACC             
BstXI         602  CCAAGAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1013  CCAGCAATTTGG        
DsaI          692  CCGTGG               C^C[GA][CT]GG
             1456  CCATGG              
EcoO65I       504  GGTCACC              G^GT[ACGT]ACC
             1636  GGTGACC             
EcoT14I       242  CCAAGG               C^C[AT][AT]GG
             1456  CCATGG              
HphI         1636  GGTGA                GGTGA(8/7)
              506  TCACC               
MluNI         611  TGGCCA               TGG^CCA
              938  TGGCCA              
MscI          611  TGGCCA               TGG^CCA
              938  TGGCCA              
MslI         1065  CACAATTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1597  CACCTTGATG          
MspA1I         88  CAGCTG               C[AC]G^C[GT]G
             1734  CAGCTG              
NspI          758  GCATGC               [GA]CATG^[CT]
             1255  GCATGT              
PflFI         441  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              859  GACCCGGTC           
PstI          379  CTGCAG               CTGCA^G
              924  CTGCAG              
PvuII          88  CAGCTG               CAG^CTG
             1734  CAGCTG              
SexAI         371  ACCTGGT              A^CC[AT]GGT
              623  ACCTGGT             
SfaNI         660  GATGC                GCATC(5/9)
              906  GATGC               
SfiI          144  GGCCGTGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              220  GGCCAGGACGGCC       
SmaI          310  CCCGGG               CCC^GGG
              356  CCCGGG              
StyI          242  CCAAGG               C^C[AT][AT]GG
             1456  CCATGG              
TfiI         1149  GAATC                G^A[AT]TC
             1212  GATTC               
Tth111I       441  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              859  GACCCGGTC           
XcmI          362  CCATCAACAACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              614  CCAACAAGTACCTGG     
XmaCI         310  CCCGGG               C^CCGGG
              356  CCCGGG              
XmaI          310  CCCGGG               C^CCGGG
              356  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BanII       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfcI        TaqI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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