Restriction map for
pF1KA1384
(Circular) (Six-base) MAPSORT of: pF1KA1384.seq Check: 224 from: 1 to: 4997
pF1KA1384 4997 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 660 660
Size: 4997
AccI GT'mk_AC
Cuts at: 2035 2035
Size: 4997
Acc65I G'GTAC_C
Cuts at: 2013 2013
Size: 4997
AclI AA'CG_TT
Cuts at: 4837 4837
Size: 4997
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1005 2822 3254 4001 4269 1005
Size: 1817 432 747 268 1733
Fragments arranged by size:
1817 1733 747 432 268
AflIII A'CryG_T
Cuts at: 2349 3453 4430 2349
Size: 1104 977 2916
Fragments arranged by size:
2916 1104 977
AgeI A'CCGG_T
Cuts at: 3385 3385
Size: 4997
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 613 613
Size: 4997
AlwNI CAG_nnn'CTG
Cuts at: 984 1044 1488 1945 2302 3869 984
Size: 60 444 457 357 1567 2112
Fragments arranged by size:
2112 1567 457 444 357 60
ApaI G_GGCC'C
Cuts at: 348 348
Size: 4997
ApaLI G'TGCA_C
Cuts at: 1510 3767 1510
Size: 2257 2740
ApoI r'AATT_y
Cuts at: 1055 1281 2001 4350 4606 1055
Size: 226 720 2349 256 1446
Fragments arranged by size:
2349 1446 720 256 226
AseI AT'TA_AT
Cuts at: 20 20
Size: 4997
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
AvaI C'yCGr_G
Cuts at: 310 418 464 523 2017 310
Size: 108 46 59 1494 3290
Fragments arranged by size:
3290 1494 108 59 46
BamHI G'GATC_C
Cuts at: 2022 2022
Size: 4997
BanI G'GyrC_C
Cuts at: 2013 2427 2675 2710 2013
Size: 414 248 35 4300
Fragments arranged by size:
4300 414 248 35
BanII G_rGCy'C
Cuts at: 348 419 1605 2011 3041 348
Size: 71 1186 406 1030 2304
Fragments arranged by size:
2304 1186 1030 406 71
BbeI G_GCGC'C
Cuts at: 2431 2679 2431
Size: 248 4749
BbsI GAAGACnn'nnnn_
Cuts at: 1162 1162
Size: 4997
BciVI GTATCCnnnnn_n'
Cuts at: 2895 3656 4997 2895
Size: 761 1341 2895
Fragments arranged by size:
2895 1341 761
BclI T'GATC_A
Cuts at: 2517 2517
Size: 4997
BfrBI ATG'CAT
Cuts at: 1363 1363
Size: 4997
BglI GCCn_nnn'nGGC
Cuts at: 259 335 1023 259
Size: 76 688 4233
Fragments arranged by size:
4233 688 76
BglII A'GATC_T
Cuts at: 647 2512 4549 647
Size: 1865 2037 1095
Fragments arranged by size:
2037 1865 1095
BlpI GC'TnA_GC
Cuts at: 2109 2109
Size: 4997
Bme1580I G_kGCm'C
Cuts at: 348 1197 1514 2622 2715 3771 348
Size: 849 317 1108 93 1056 1574
Fragments arranged by size:
1574 1108 1056 849 317 93
BmgBI CAC'GTC
Cuts at: 210 210
Size: 4997
BmrI ACTGGGnnnn_n'
Cuts at: 1579 2209 2290 2625 4769 1579
Size: 630 81 335 2144 1807
Fragments arranged by size:
2144 1807 630 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3364 3364
Size: 4997
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 721 865 1866 279
Size: 442 144 1001 3410
Fragments arranged by size:
3410 1001 442 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2173 3052 3544 3842 2173
Size: 879 492 298 3328
Fragments arranged by size:
3328 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 700 853 4701 700
Size: 153 3848 996
Fragments arranged by size:
3848 996 153
BsaAI yAC'GTr
Cuts at: 1389 2350 1389
Size: 961 4036
BsaBI GATnn'nnATC
Cuts at: 6 2511 4548 6
Size: 2505 2037 455
Fragments arranged by size:
2505 2037 455
BsaHI Gr'CG_yC
Cuts at: 995 2428 2676 3378 4874 995
Size: 1433 248 702 1496 1118
Fragments arranged by size:
1496 1433 1118 702 248
BsaWI w'CCGG_w
Cuts at: 1107 2186 2707 3385 3659 3806 4217 1107
Size: 1079 521 678 274 147 411 1887
Fragments arranged by size:
1887 1079 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 115 145 565 595 1544 1574 115
Size: 30 420 30 949 30 3538
Fragments arranged by size:
3538 949 420 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 427 586 718 778 427
Size: 159 132 60 4646
Fragments arranged by size:
4646 159 132 60
BseYI C'CCAG_C
Cuts at: 145 1120 2422 3180 3757 4253 145
Size: 975 1302 758 577 496 889
Fragments arranged by size:
1302 975 889 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 583 649 676 1823 151
Size: 432 66 27 1147 3325
Fragments arranged by size:
3325 1147 432 66 27
BsiEI CG_ry'CG
Cuts at: 104 286 855 2539 2585 3793 104
Size: 182 569 1684 46 1208 1308
Fragments arranged by size:
1684 1308 1208 569 182 46
BsiHKAI G_wGCw'C
Cuts at: 524 851 1514 2011 2789 2979 3771 524
Size: 327 663 497 778 190 792 1750
Fragments arranged by size:
1750 792 778 663 497 327 190
BsmI GAATG_Cn'
Cuts at: 1732 1732
Size: 4997
Bsp1286I G_dGCh'C
Cuts at: 348 419 524 851 1197 1514 1605 2011 2622
Size: 71 105 327 346 317 91 406 611
Cuts at: 2622 2715 2789 2979 3041 3771 348
Size: 93 74 190 62 730 1574
Fragments arranged by size:
1574 730 611 406 346 327 317 190
105 93 91 74 71 62
BspEI T'CCGG_A
Cuts at: 4217 4217
Size: 4997
BspHI T'CATG_A
Cuts at: 887 4173 887
Size: 3286 1711
BspMI ACCTGCnnnn'nnnn_
Cuts at: 170 188 660 2047 2261 2563 2944 170
Size: 18 472 1387 214 302 381 2223
Fragments arranged by size:
2223 1387 472 381 302 214 18
BsrBI CCG'CTC
Cuts at: 50 936 3289 3343 4827 4992 50
Size: 886 2353 54 1484 165 55
Fragments arranged by size:
2353 1484 886 165 55 54
BsrDI GCAATG_nn'
Cuts at: 240 2909 240
Size: 2669 2328
BsrFI r'CCGG_y
Cuts at: 1112 2995 3176 3385 1112
Size: 1883 181 209 2724
Fragments arranged by size:
2724 1883 209 181
BsrGI T'GTAC_A
Cuts at: 1542 1542
Size: 4997
BssHII G'CGCG_C
Cuts at: 771 771
Size: 4997
BssSI C'ACGA_G
Cuts at: 3268 3626 3268
Size: 358 4639
BstAPI GCAn_nnn'nTGC
Cuts at: 1945 2374 1945
Size: 429 4568
BstBI TT'CG_AA
Cuts at: 3358 3358
Size: 4997
BstEII G'GTnAC_C
Cuts at: 612 1744 612
Size: 1132 3865
BstXI CCAn_nnnn'nTGG
Cuts at: 717 1128 717
Size: 411 4586
BstYI r'GATC_y
Cuts at: 1 115 593 647 2022 2489 2512 2847 3093
Size: 114 478 54 1375 467 23 335 246
Cuts at: 3093 4105 4191 4203 4549 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1375 1012 478 467 449 346 335 246
114 86 54 23 12
BtgI C'CryG_G
Cuts at: 800 1564 800
Size: 764 4233
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2947 3128 3291 2947
Size: 181 163 4653
Fragments arranged by size:
4653 181 163
BtsI GCAGTG_nn'
Cuts at: 1029 1200 1514 1811 2240 1029
Size: 171 314 297 429 3786
Fragments arranged by size:
3786 429 314 297 171
DraI TTT'AAA
Cuts at: 1996 1996
Size: 4997
DrdI GACnn_nn'nnGTC
Cuts at: 2703 3561 2703
Size: 858 4139
EaeI y'GGCC_r
Cuts at: 327 539 719 1046 2582 2756 3147 3174 4519
Size: 212 180 327 1536 174 391 27 1345
Cuts at: 4519 327
Size: 805
Fragments arranged by size:
1536 1345 805 391 327 212 180 174
27
EagI C'GGCC_G
Cuts at: 2582 2582
Size: 4997
EarI CTCTTCn'nnn_
Cuts at: 295 826 1341 3020 3230 295
Size: 531 515 1679 210 2062
Fragments arranged by size:
2062 1679 531 515 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3205 3515 3661 4807 3205
Size: 310 146 1146 3395
Fragments arranged by size:
3395 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2009 2009
Size: 4997
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 721 865 1005 1866 2822 3254 4001 4269
Size: 442 144 140 861 956 432 747 268
Cuts at: 4269 279
Size: 1007
Fragments arranged by size:
1007 956 861 747 442 432 268 144
140
EcoNI CCTnn'n_nnAGG
Cuts at: 736 736
Size: 4997
EcoO109I rG'GnC_Cy
Cuts at: 128 344 345 1856 1942 2136 128
Size: 216 1 1511 86 194 2989
Fragments arranged by size:
2989 1511 216 194 86 1
EcoRI G'AATT_C
Cuts at: 1055 2001 1055
Size: 946 4051
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3016 3016
Size: 4997
FspI TGC'GCA
Cuts at: 2778 2778
Size: 4997
HaeII r_GCGC'y
Cuts at: 820 897 1464 2431 2679 3701 4680 820
Size: 77 567 967 248 1022 979 1137
Fragments arranged by size:
1137 1022 979 967 567 248 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 115 147 565 597 634 666 1544 1576 2839
Size: 32 418 32 37 32 878 32 1263
Cuts at: 2839 2869 2871 2901 115
Size: 30 2 30 2211
Fragments arranged by size:
2211 1263 878 418 37 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2036 2036
Size: 4997
KasI G'GCGC_C
Cuts at: 2427 2675 2427
Size: 248 4749
KpnI G_GTAC'C
Cuts at: 2017 2017
Size: 4997
MfeI C'AATT_G
Cuts at: 1673 1673
Size: 4997
MluI A'CGCG_T
Cuts at: 4430 4430
Size: 4997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 788 1068 1352 3668 3852 788
Size: 280 284 2316 184 1933
Fragments arranged by size:
2316 1933 284 280 184
MscI TGG'CCA
Cuts at: 721 1048 2758 721
Size: 327 1710 2960
Fragments arranged by size:
2960 1710 327
MslI CAynn'nnrTG
Cuts at: 1177 1709 2986 3113 1177
Size: 532 1277 127 3061
Fragments arranged by size:
3061 1277 532 127
MspA1I CmG'CkG
Cuts at: 198 1844 2108 2422 2782 3795 4040 198
Size: 1646 264 314 360 1013 245 1155
Fragments arranged by size:
1646 1155 1013 360 314 264 245
NaeI GCC'GGC
Cuts at: 3178 3178
Size: 4997
NarI GG'CG_CC
Cuts at: 2428 2676 2428
Size: 248 4749
NcoI C'CATG_G
Cuts at: 1564 1564
Size: 4997
NgoMIV G'CCGG_C
Cuts at: 3176 3176
Size: 4997
NsiI A_TGCA'T
Cuts at: 1365 1365
Size: 4997
NspI r_CATG'y
Cuts at: 870 1367 2050 2550 3457 870
Size: 497 683 500 907 2410
Fragments arranged by size:
2410 907 683 500 497
PasI CC'CwG_GG
Cuts at: 225 225
Size: 4997
PciI A'CATG_T
Cuts at: 3453 3453
Size: 4997
PflMI CCAn_nnn'nTGG
Cuts at: 948 948
Size: 4997
PfoI T'CCnGG_A
Cuts at: 1101 1101
Size: 4997
PmeI GTTT'AAAC
Cuts at: 1996 1996
Size: 4997
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 4136
Size: 32 4965
PpuMI rG'GwC_Cy
Cuts at: 128 128
Size: 4997
PspOMI G'GGCC_C
Cuts at: 344 344
Size: 4997
PspXI vC'TCGA_Gb
Cuts at: 523 523
Size: 4997
PstI C_TGCA'G
Cuts at: 491 1036 2044 2729 491
Size: 545 1008 685 2759
Fragments arranged by size:
2759 1008 685 545
PvuI CG_AT'CG
Cuts at: 104 286 104
Size: 182 4815
PvuII CAG'CTG
Cuts at: 198 1844 2422 2782 198
Size: 1646 578 360 2413
Fragments arranged by size:
2413 1646 578 360
RsrII CG'GwC_CG
Cuts at: 1110 3192 1110
Size: 2082 2915
SacI G_AGCT'C
Cuts at: 2011 2011
Size: 4997
SalI G'TCGA_C
Cuts at: 2034 2034
Size: 4997
SapI GCTCTTCn'nnn_
Cuts at: 295 826 1341 3020 3230 295
Size: 531 515 1679 210 2062
Fragments arranged by size:
2062 1679 531 515 210
SbfI CC_TGCA'GG
Cuts at: 2044 2044
Size: 4997
ScaI AGT'ACT
Cuts at: 277 4514 277
Size: 4237 760
SexAI A'CCwGG_T
Cuts at: 479 731 479
Size: 252 4745
SfcI C'TryA_G
Cuts at: 33 487 1032 1839 2040 2725 3718 3909 33
Size: 454 545 807 201 685 993 191 1121
Fragments arranged by size:
1121 993 807 685 545 454 201 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 259 335 259
Size: 76 4921
SfoI GGC'GCC
Cuts at: 2429 2677 2429
Size: 248 4749
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
SmaI CCC'GGG
Cuts at: 420 466 2019 420
Size: 46 1553 3398
Fragments arranged by size:
3398 1553 46
SmlI C'TyrA_G
Cuts at: 523 2152 3067 3559 3821 523
Size: 1629 915 492 262 1699
Fragments arranged by size:
1699 1629 915 492 262
SnaBI TAC'GTA
Cuts at: 1389 1389
Size: 4997
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4997
SphI G_CATG'C
Cuts at: 870 2050 2550 870
Size: 1180 500 3317
Fragments arranged by size:
3317 1180 500
StyI C'CwwG_G
Cuts at: 350 1564 2131 2485 350
Size: 1214 567 354 2862
Fragments arranged by size:
2862 1214 567 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 573 1228 1347 2580 2713 3172 3381 573
Size: 655 119 1233 133 459 209 2189
Fragments arranged by size:
2189 1233 655 459 209 133 119
TatI w'GTAC_w
Cuts at: 275 1276 1542 4512 275
Size: 1001 266 2970 760
Fragments arranged by size:
2970 1001 760 266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4514 4546 4514
Size: 32 4965
Tth111I GACn'n_nGTC
Cuts at: 552 970 2794 552
Size: 418 1824 2755
Fragments arranged by size:
2755 1824 418
XbaI T'CTAG_A
Cuts at: 2028 2028
Size: 4997
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 477 729 2492 477
Size: 252 1763 2982
Fragments arranged by size:
2982 1763 252
XhoI C'TCGA_G
Cuts at: 523 523
Size: 4997
XmaI C'CCGG_G
Cuts at: 418 464 2017 418
Size: 46 1553 3398
Fragments arranged by size:
3398 1553 46
XmnI GAAnn'nnTTC
Cuts at: 1696 1696
Size: 4997
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BmtI Bpu10I BsiWI BsmBI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI PacI PmlI PshAI
PsiI PsrI SacII SanDI SgrAI SrfI SspI StuI
SwaI ZraI