Restriction map for pF1KA1384
 (Linear) (Six-base) MAPSORT of: KIAA1384.seq  Check: 2249  from: 1  to: 1884



KIAA1384.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     552    1884 
   Size:        552    1332
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     897    1884 
   Size:        897     987
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     505    1884 
   Size:        505    1379
 
AlwNI CAG_nnn'CTG

Cuts at:      0     876     936    1380    1837    1884 
   Size:        876      60     444     457      47

  Fragments arranged by size: 

                 876     457     444      60      47
 
ApaI G_GGCC'C

Cuts at:      0     240    1884 
   Size:        240    1644
 
ApaLI G'TGCA_C

Cuts at:      0    1402    1884 
   Size:       1402     482
 
ApoI r'AATT_y

Cuts at:      0     947    1173    1884 
   Size:        947     226     711

  Fragments arranged by size: 

                 947     711     226
 
AvaI C'yCGr_G

Cuts at:      0     202     310     356     415    1884 
   Size:        202     108      46      59    1469

  Fragments arranged by size: 

                1469     202     108      59      46
 
BanII G_rGCy'C

Cuts at:      0     240     311    1497    1884 
   Size:        240      71    1186     387

  Fragments arranged by size: 

                1186     387     240      71
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1054    1884 
   Size:       1054     830
 
BfrBI ATG'CAT

Cuts at:      0    1255    1884 
   Size:       1255     629
 
BglI GCCn_nnn'nGGC

Cuts at:      0     151     227     915    1884 
   Size:        151      76     688     969

  Fragments arranged by size: 

                 969     688     151      76
 
BglII A'GATC_T

Cuts at:      0     539    1884 
   Size:        539    1345
 
Bme1580I G_kGCm'C

Cuts at:      0     240    1089    1406    1884 
   Size:        240     849     317     478

  Fragments arranged by size: 

                 849     478     317     240
 
BmgBI CAC'GTC

Cuts at:      0     102    1884 
   Size:        102    1782
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1471    1884 
   Size:       1471     413
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     613     757    1758    1884 
   Size:        171     442     144    1001     126

  Fragments arranged by size: 

                1001     442     171     144     126
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     592     745    1884 
   Size:        592     153    1139

  Fragments arranged by size: 

                1139     592     153
 
BsaAI yAC'GTr

Cuts at:      0    1281    1884 
   Size:       1281     603
 
BsaHI Gr'CG_yC

Cuts at:      0     887    1884 
   Size:        887     997
 
BsaWI w'CCGG_w

Cuts at:      0     999    1884 
   Size:        999     885
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       7      37     457     487    1436    1466    1884 
   Size:          7      30     420      30     949      30     418

  Fragments arranged by size: 

                 949     420     418      30      30      30       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     319     478     610     670    1884 
   Size:        319     159     132      60    1214

  Fragments arranged by size: 

                1214     319     159     132      60
 
BseYI C'CCAG_C

Cuts at:      0      37    1012    1884 
   Size:         37     975     872

  Fragments arranged by size: 

                 975     872      37
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     475     541     568    1715    1884 
   Size:         43     432      66      27    1147     169

  Fragments arranged by size: 

                1147     432     169      66      43      27
 
BsiEI CG_ry'CG

Cuts at:      0     178     747    1884 
   Size:        178     569    1137

  Fragments arranged by size: 

                1137     569     178
 
BsiHKAI G_wGCw'C

Cuts at:      0     416     743    1406    1884 
   Size:        416     327     663     478

  Fragments arranged by size: 

                 663     478     416     327
 
BsmI GAATG_Cn'

Cuts at:      0    1624    1884 
   Size:       1624     260
 
Bsp1286I G_dGCh'C

Cuts at:      0     240     311     416     743    1089    1406    1497    1884 
   Size:        240      71     105     327     346     317      91     387

  Fragments arranged by size: 

                 387     346     327     317     240     105      91      71
            
 
BspHI T'CATG_A

Cuts at:      0     779    1884 
   Size:        779    1105
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      62      80     552    1884 
   Size:         62      18     472    1332

  Fragments arranged by size: 

                1332     472      62      18
 
BsrBI CCG'CTC

Cuts at:      0     828    1884 
   Size:        828    1056
 
BsrDI GCAATG_nn'

Cuts at:      0     132    1884 
   Size:        132    1752
 
BsrFI r'CCGG_y

Cuts at:      0    1004    1884 
   Size:       1004     880
 
BsrGI T'GTAC_A

Cuts at:      0    1434    1884 
   Size:       1434     450
 
BssHII G'CGCG_C

Cuts at:      0     663    1884 
   Size:        663    1221
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1837    1884 
   Size:       1837      47
 
BstEII G'GTnAC_C

Cuts at:      0     504    1636    1884 
   Size:        504    1132     248

  Fragments arranged by size: 

                1132     504     248
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     609    1020    1884 
   Size:        609     411     864

  Fragments arranged by size: 

                 864     609     411
 
BstYI r'GATC_y

Cuts at:      0       7     485     539    1884 
   Size:          7     478      54    1345

  Fragments arranged by size: 

                1345     478      54       7
 
BtgI C'CryG_G

Cuts at:      0     692    1456    1884 
   Size:        692     764     428

  Fragments arranged by size: 

                 764     692     428
 
BtsI GCAGTG_nn'

Cuts at:      0     921    1092    1406    1703    1884 
   Size:        921     171     314     297     181

  Fragments arranged by size: 

                 921     314     297     181     171
 
EaeI y'GGCC_r

Cuts at:      0     219     431     611     938    1884 
   Size:        219     212     180     327     946

  Fragments arranged by size: 

                 946     327     219     212     180
 
EarI CTCTTCn'nnn_

Cuts at:      0     187     718    1233    1884 
   Size:        187     531     515     651

  Fragments arranged by size: 

                 651     531     515     187
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     613     757     897    1758    1884 
   Size:        171     442     144     140     861     126

  Fragments arranged by size: 

                 861     442     171     144     140     126
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     628    1884 
   Size:        628    1256
 
EcoO109I rG'GnC_Cy

Cuts at:      0      20     236     237    1748    1834    1884 
   Size:         20     216       1    1511      86      50

  Fragments arranged by size: 

                1511     216      86      50      20       1
 
EcoRI G'AATT_C

Cuts at:      0     947    1884 
   Size:        947     937
 
HaeII r_GCGC'y

Cuts at:      0     712     789    1356    1884 
   Size:        712      77     567     528

  Fragments arranged by size: 

                 712     567     528      77
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       7      39     457     489     526     558    1436    1468 
   Size:          7      32     418      32      37      32     878      32

Cuts at:   1468    1884 
   Size:        416

  Fragments arranged by size: 

                 878     418     416      37      32      32      32      32
                   7
 
MfeI C'AATT_G

Cuts at:      0    1565    1884 
   Size:       1565     319
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     680     960    1244    1884 
   Size:        680     280     284     640

  Fragments arranged by size: 

                 680     640     284     280
 
MscI TGG'CCA

Cuts at:      0     613     940    1884 
   Size:        613     327     944

  Fragments arranged by size: 

                 944     613     327
 
MslI CAynn'nnrTG

Cuts at:      0    1069    1601    1884 
   Size:       1069     532     283

  Fragments arranged by size: 

                1069     532     283
 
MspA1I CmG'CkG

Cuts at:      0      90    1736    1884 
   Size:         90    1646     148

  Fragments arranged by size: 

                1646     148      90
 
NcoI C'CATG_G

Cuts at:      0    1456    1884 
   Size:       1456     428
 
NsiI A_TGCA'T

Cuts at:      0    1257    1884 
   Size:       1257     627
 
NspI r_CATG'y

Cuts at:      0     762    1259    1884 
   Size:        762     497     625

  Fragments arranged by size: 

                 762     625     497
 
PasI CC'CwG_GG

Cuts at:      0     117    1884 
   Size:        117    1767
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     840    1884 
   Size:        840    1044
 
PfoI T'CCnGG_A

Cuts at:      0     993    1884 
   Size:        993     891
 
PpuMI rG'GwC_Cy

Cuts at:      0      20    1884 
   Size:         20    1864
 
PspOMI G'GGCC_C

Cuts at:      0     236    1884 
   Size:        236    1648
 
PspXI vC'TCGA_Gb

Cuts at:      0     415    1884 
   Size:        415    1469
 
PstI C_TGCA'G

Cuts at:      0     383     928    1884 
   Size:        383     545     956

  Fragments arranged by size: 

                 956     545     383
 
PvuI CG_AT'CG

Cuts at:      0     178    1884 
   Size:        178    1706
 
PvuII CAG'CTG

Cuts at:      0      90    1736    1884 
   Size:         90    1646     148

  Fragments arranged by size: 

                1646     148      90
 
RsrII CG'GwC_CG

Cuts at:      0    1002    1884 
   Size:       1002     882
 
SapI GCTCTTCn'nnn_

Cuts at:      0     187     718    1233    1884 
   Size:        187     531     515     651

  Fragments arranged by size: 

                 651     531     515     187
 
ScaI AGT'ACT

Cuts at:      0     169    1884 
   Size:        169    1715
 
SexAI A'CCwGG_T

Cuts at:      0     371     623    1884 
   Size:        371     252    1261

  Fragments arranged by size: 

                1261     371     252
 
SfcI C'TryA_G

Cuts at:      0     379     924    1731    1884 
   Size:        379     545     807     153

  Fragments arranged by size: 

                 807     545     379     153
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     151     227    1884 
   Size:        151      76    1657

  Fragments arranged by size: 

                1657     151      76
 
SmaI CCC'GGG

Cuts at:      0     312     358    1884 
   Size:        312      46    1526

  Fragments arranged by size: 

                1526     312      46
 
SmlI C'TyrA_G

Cuts at:      0     415    1884 
   Size:        415    1469
 
SnaBI TAC'GTA

Cuts at:      0    1281    1884 
   Size:       1281     603
 
SphI G_CATG'C

Cuts at:      0     762    1884 
   Size:        762    1122
 
StyI C'CwwG_G

Cuts at:      0     242    1456    1884 
   Size:        242    1214     428

  Fragments arranged by size: 

                1214     428     242
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     465    1120    1239    1884 
   Size:        465     655     119     645

  Fragments arranged by size: 

                 655     645     465     119
 
TatI w'GTAC_w

Cuts at:      0     167    1168    1434    1884 
   Size:        167    1001     266     450

  Fragments arranged by size: 

                1001     450     266     167
 
Tth111I GACn'n_nGTC

Cuts at:      0     444     862    1884 
   Size:        444     418    1022

  Fragments arranged by size: 

                1022     444     418
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     369     621    1884 
   Size:        369     252    1263

  Fragments arranged by size: 

                1263     369     252
 
XhoI C'TCGA_G

Cuts at:      0     415    1884 
   Size:        415    1469
 
XmaI C'CCGG_G

Cuts at:      0     310     356    1884 
   Size:        310      46    1528

  Fragments arranged by size: 

                1528     310      46
 
XmnI GAAnn'nnTTC

Cuts at:      0    1588    1884 
   Size:       1588     296
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI
    BanII     BbsI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI
     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII   BstAPI   BstEII    BstXI    BstYI
     BtgI     BtsI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI
    HaeII    Hin4I     MfeI     MmeI     MscI     MslI   MspA1I     NcoI
     NsiI     NspI     PasI    PflMI     PfoI    PpuMI   PspOMI    PspXI
     PstI     PvuI    PvuII    RsrII     SapI     ScaI    SexAI     SfcI
     SfiI     SmaI     SmlI    SnaBI     SphI     StyI    TaqII     TatI
  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI     BlpI
     BmtI     BplI   Bpu10I    BpuEI    BsaBI    BsiWI    BsmBI    BspEI
    BssSI    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI     PsrI     SacI
    SacII     SalI    SanDI     SbfI     SfoI     SgfI    SgrAI     SpeI
     SrfI     SspI     StuI     SwaI     TstI     XbaI     ZraI
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