Restriction map for pF1KA1373
 (Linear) (Six-base) MAPSORT of: KIAA1373.seq  Check: 850  from: 1  to: 1383



KIAA1373.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349    1149    1383 
   Size:        349     800     234

  Fragments arranged by size: 

                 800     349     234
 
AfeI AGC'GCT

Cuts at:      0     627    1383 
   Size:        627     756
 
AlwNI CAG_nnn'CTG

Cuts at:      0     301     531    1383 
   Size:        301     230     852

  Fragments arranged by size: 

                 852     301     230
 
ApoI r'AATT_y

Cuts at:      0      21     207     238     437     777     832     913    1261 
   Size:         21     186      31     199     340      55      81     348

Cuts at:   1261    1383 
   Size:        122

  Fragments arranged by size: 

                 348     340     199     186     122      81      55      31
                  21
 
AvaI C'yCGr_G

Cuts at:      0     569    1383 
   Size:        569     814
 
BamHI G'GATC_C

Cuts at:      0    1034    1383 
   Size:       1034     349
 
BanII G_rGCy'C

Cuts at:      0    1241    1307    1383 
   Size:       1241      66      76

  Fragments arranged by size: 

                1241      76      66
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     137    1383 
   Size:        137    1246
 
BglII A'GATC_T

Cuts at:      0     819    1383 
   Size:        819     564
 
Bme1580I G_kGCm'C

Cuts at:      0      96    1383 
   Size:         96    1287
 
BmtI G_CTAG'C

Cuts at:      0     719    1383 
   Size:        719     664
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182    1383 
   Size:        182    1201
 
Bpu10I CC'TnA_GC

Cuts at:      0      70      97    1383 
   Size:         70      27    1286

  Fragments arranged by size: 

                1286      70      27
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     535    1210    1383 
   Size:        535     675     173

  Fragments arranged by size: 

                 675     535     173
 
BsaHI Gr'CG_yC

Cuts at:      0     184    1383 
   Size:        184    1199
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1001    1031    1383 
   Size:       1001      30     352

  Fragments arranged by size: 

                1001     352      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     716    1009    1383 
   Size:        716     293     374

  Fragments arranged by size: 

                 716     374     293
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314    1383 
   Size:        314    1069
 
BsmI GAATG_Cn'

Cuts at:      0     340    1383 
   Size:        340    1043
 
Bsp1286I G_dGCh'C

Cuts at:      0      96    1241    1307    1383 
   Size:         96    1145      66      76

  Fragments arranged by size: 

                1145      96      76      66
 
BsrDI GCAATG_nn'

Cuts at:      0     295    1121    1383 
   Size:        295     826     262

  Fragments arranged by size: 

                 826     295     262
 
BstBI TT'CG_AA

Cuts at:      0     539    1383 
   Size:        539     844
 
BstYI r'GATC_y

Cuts at:      0     819    1034    1349    1383 
   Size:        819     215     315      34

  Fragments arranged by size: 

                 819     315     215      34
 
Bsu36I CC'TnA_GG

Cuts at:      0     597    1383 
   Size:        597     786
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     813    1383 
   Size:        813     570
 
BtsI GCAGTG_nn'

Cuts at:      0    1286    1383 
   Size:       1286      97
 
DraI TTT'AAA

Cuts at:      0     377    1383 
   Size:        377    1006
 
EarI CTCTTCn'nnn_

Cuts at:      0      74    1383 
   Size:         74    1309
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     349    1149    1383 
   Size:        182     167     800     234

  Fragments arranged by size: 

                 800     234     182     167
 
EcoO109I rG'GnC_Cy

Cuts at:      0     740    1383 
   Size:        740     643
 
EcoRI G'AATT_C

Cuts at:      0      21    1383 
   Size:         21    1362
 
HaeII r_GCGC'y

Cuts at:      0     629    1383 
   Size:        629     754
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1325    1357    1383 
   Size:       1325      32      26

  Fragments arranged by size: 

                1325      32      26
 
HindIII A'AGCT_T

Cuts at:      0     103     262    1383 
   Size:        103     159    1121

  Fragments arranged by size: 

                1121     159     103
 
MslI CAynn'nnrTG

Cuts at:      0      59    1383 
   Size:         59    1324
 
MspA1I CmG'CkG

Cuts at:      0     754    1383 
   Size:        754     629
 
NheI G'CTAG_C

Cuts at:      0     715    1383 
   Size:        715     668
 
NspI r_CATG'y

Cuts at:      0    1188    1383 
   Size:       1188     195
 
PsiI TTA'TAA

Cuts at:      0     233     244    1383 
   Size:        233      11    1139

  Fragments arranged by size: 

                1139     233      11
 
PvuII CAG'CTG

Cuts at:      0     754    1383 
   Size:        754     629
 
SapI GCTCTTCn'nnn_

Cuts at:      0      74    1383 
   Size:         74    1309
 
SmlI C'TyrA_G

Cuts at:      0     550    1189    1383 
   Size:        550     639     194

  Fragments arranged by size: 

                 639     550     194
 
SphI G_CATG'C

Cuts at:      0    1188    1383 
   Size:       1188     195
 
SspI AAT'ATT

Cuts at:      0     406    1383 
   Size:        406     977
 
StyI C'CwwG_G

Cuts at:      0     346    1232    1383 
   Size:        346     886     151

  Fragments arranged by size: 

                 886     346     151
 
XmnI GAAnn'nnTTC

Cuts at:      0     263    1383 
   Size:        263    1120
 

 Enzymes that do cut:

     AcuI     AfeI    AlwNI     ApoI     AvaI    BamHI    BanII     BbsI
    BglII Bme1580I     BmtI     BpmI   Bpu10I    BpuEI    BsaHI    BsaXI
    BseRI     BsgI     BsmI Bsp1286I    BsrDI    BstBI    BstYI   Bsu36I
    BtgZI     BtsI     DraI     EarI  Eco57MI EcoO109I    EcoRI    HaeII
    Hin4I  HindIII     MslI   MspA1I     NheI     NspI     PsiI    PvuII
     SapI     SmlI     SphI     SspI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BplI     BsaI
    BsaAI    BsaBI    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI  BstZ17I     BtgI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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