Restriction map for pF1KA1321
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstUI       BstXI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco52I      
EcoNI       EcoRI       EcoT22I     FauI        FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SgrAI       SmaI        SnaBI       Sse8387I    SspBI       
SspI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccI          391  GTAGAC               GT^[AC][GT]AC
Aor51HI      1212  AGCGCT               AGC^GCT
Asp700I      1648  GAAATAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1449  GAGCAC               G[AT]GC[AT]^C
BanII        1540  GAGCCC               G[GA]GC[CT]^C
BsaI          742  GAGACC               GGTCTC(1/5)
BseRI           4  GAGGAG               GAGGAG(10/8)
BsgI          718  GTGCAG               GTGCAG(16/14)
BsiHKAI      1449  GAGCAC               G[AT]GC[AT]^C
BsmI         1733  GCATTC               GAATGC(1/-1)
BsrBI         645  GAGCGG               CCGCTC(-3/-3)
BssSI         996  CTCGTG               CACGAG(-5/-1)
BstAPI       1434  GCAAACTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         825  TTCGAA               TT^CGAA
BstEII       1795  GGTGACC              G^GT[ACGT]ACC
BstPI        1795  GGTGACC              G^GT[ACGT]ACC
DraIII       1637  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         2066  CCATGG               C^C[GA][CT]GG
EaeI           15  CGGCCA               [CT]^GGCC[GA]
Eco47III     1212  AGCGCT               AGC^GCT
EcoO65I      1795  GGTGACC              G^GT[ACGT]ACC
EcoRV        1501  GATATC               GAT^ATC
HaeII        1212  AGCGCT               [GA]GCGC^[CT]
MslI         1789  CATATAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         2066  CCATGG               C^CATGG
NspI          966  GCATGC               [GA]CATG^[CT]
NspV          825  TTCGAA               TT^CGAA
SexAI         921  ACCTGGT              A^CC[AT]GGT
SfaNI        1671  GCATC                GCATC(5/9)
SfuI          825  TTCGAA               TT^CGAA
SmlI         1718  CTCAAG               C^T[CT][GA]AG
SphI          966  GCATGC               GCATG^C
StuI         1935  AGGCCT               AGG^CCT
XmnI         1648  GAAATAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcsI          456  GAATTT               [GA]^AATT[CT]
             2019  GAATTT              
AocI         1658  CCTCAGG              CC^T[ACGT]AGG
             1971  CCTGAGG             
ApoI          456  GAATTT               [GA]^AATT[CT]
             2019  GAATTT              
BbvCI          28  CCTCAGC              CCTCAGC(-5/-2)
               67  CCTCAGC             
BcnI           13  CCCGG                CC^[GC]GG
             1160  CCGGG               
BmyI         1449  GAGCAC               G[AGT]GC[ACT]^C
             1540  GAGCCC              
BsmAI         742  GAGAC                GTCTC(1/5)
             1558  GAGAC               
Bsp1286I     1449  GAGCAC               G[AGT]GC[ACT]^C
             1540  GAGCCC              
BstF5I         52  CATCC                GGATG(2/0)
             1672  CATCC               
Bsu36I       1658  CCTCAGG              CC^T[ACGT]AGG
             1971  CCTGAGG             
CfoI          681  GCGC                 GCG^C
             1213  GCGC                
DraI          321  TTTAAA               TTT^AAA
             1009  TTTAAA              
DraII         365  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             1199  AGGTCCC             
EarI         1116  CTCTTC               CTCTTC(1/4)
             1338  CTCTTC              
Eco81I       1658  CCTCAGG              CC^T[ACGT]AGG
             1971  CCTGAGG             
EcoO109I      365  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             1199  AGGTCCC             
FokI           52  CATCC                GGATG(9/13)
             1672  CATCC               
HapII          14  CCGG                 C^CGG
             1160  CCGG                
HhaI          681  GCGC                 GCG^C
             1213  GCGC                
HinP1I        681  GCGC                 G^CGC
             1213  GCGC                
HpaII          14  CCGG                 C^CGG
             1160  CCGG                
Ksp632I      1116  CTCTTC               CTCTTC(1/4)
             1338  CTCTTC              
MspI           14  CCGG                 C^CGG
             1160  CCGG                
NciI           13  CCCGG                CC^[GC]GG
             1160  CCGGG               
PleI          994  GACTC                GAGTC(4/5)
             1371  GAGTC               
PpuMI         365  GGGTCCT              [GA]G^G[AT]CC[CT]
             1199  AGGTCCC             
PshAI         744  GACCAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1366  GACATGAGTC          
PstI          172  CTGCAG               CTGCA^G
             1801  CTGCAG              
PvuII         974  CAGCTG               CAG^CTG
             1385  CAGCTG              
SpeI         1711  ACTAGT               A^CTAGT
             1753  ACTAGT              


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AvaII       
BbvI        BfaI        BpmI        BsaJI       BsiYI       
BslI        BsmFI       BspMI       BsrI        BssKI       
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EcoRII      EcoT14I     Fnu4HI      HaeIII      
HinfI       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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