Restriction map for pF1KA1254
 (Linear) (Six-base) MAPSORT of: KIAA1254.seq  Check: 9154  from: 1  to: 2271



KIAA1254.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AccI GT'mk_AC

Cuts at:      0     430    2271 
   Size:        430    1841
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545     590     887    2271 
   Size:        545      45     297    1384

  Fragments arranged by size: 

                1384     545     297      45
 
AflII C'TTAA_G

Cuts at:      0    1060    2271 
   Size:       1060    1211
 
AflIII A'CryG_T

Cuts at:      0    1988    2271 
   Size:       1988     283
 
AleI CACnn'nnGTG

Cuts at:      0    1939    2271 
   Size:       1939     332
 
AlwNI CAG_nnn'CTG

Cuts at:      0     451     568    2271 
   Size:        451     117    1703

  Fragments arranged by size: 

                1703     451     117
 
ApoI r'AATT_y

Cuts at:      0       9      81    1491    1570    1804    1821    1860    1963 
   Size:          9      72    1410      79     234      17      39     103

Cuts at:   1963    2271 
   Size:        308

  Fragments arranged by size: 

                1410     308     234     103      79      72      39      17
                   9
 
BanI G'GyrC_C

Cuts at:      0    1725    2271 
   Size:       1725     546
 
BanII G_rGCy'C

Cuts at:      0     113     412    2271 
   Size:        113     299    1859

  Fragments arranged by size: 

                1859     299     113
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     281     401     553     578    2271 
   Size:        281     120     152      25    1693

  Fragments arranged by size: 

                1693     281     152     120      25
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     945    1998    2271 
   Size:        945    1053     273

  Fragments arranged by size: 

                1053     945     273
 
BclI T'GATC_A

Cuts at:      0    2031    2076    2271 
   Size:       2031      45     195

  Fragments arranged by size: 

                2031     195      45
 
BglI GCCn_nnn'nGGC

Cuts at:      0     188    2271 
   Size:        188    2083
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     506     783    2271 
   Size:        506     277    1488

  Fragments arranged by size: 

                1488     506     277
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     961    1177    1183    2271 
   Size:        961     216       6    1088

  Fragments arranged by size: 

                1088     961     216       6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346    2271 
   Size:        346    1925
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     646    2271 
   Size:        646    1625
 
BsaBI GATnn'nnATC

Cuts at:      0     676    2271 
   Size:        676    1595
 
BsaHI Gr'CG_yC

Cuts at:      0     531    2271 
   Size:        531    1740
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1108    1138    2271 
   Size:       1108      30    1133

  Fragments arranged by size: 

                1133    1108      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     157    2271 
   Size:        157    2114
 
BsiHKAI G_wGCw'C

Cuts at:      0    1908    2271 
   Size:       1908     363
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     412    1908    2271 
   Size:        113     299    1496     363

  Fragments arranged by size: 

                1496     363     299     113
 
BspHI T'CATG_A

Cuts at:      0      54    2271 
   Size:         54    2217
 
BsrDI GCAATG_nn'

Cuts at:      0     154    2271 
   Size:        154    2117
 
BsrFI r'CCGG_y

Cuts at:      0     575    2271 
   Size:        575    1696
 
BstBI TT'CG_AA

Cuts at:      0    1319    2271 
   Size:       1319     952
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     703    2271 
   Size:        703    1568
 
BstZ17I GTA'TAC

Cuts at:      0     431    2271 
   Size:        431    1840
 
DraI TTT'AAA

Cuts at:      0    1608    1800    2271 
   Size:       1608     192     471

  Fragments arranged by size: 

                1608     471     192
 
EaeI y'GGCC_r

Cuts at:      0     693    2271 
   Size:        693    1578
 
EarI CTCTTCn'nnn_

Cuts at:      0     417     971    1070    2092    2271 
   Size:        417     554      99    1022     179

  Fragments arranged by size: 

                1022     554     417     179      99
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545     590     887     961    1177    1183    2271 
   Size:        545      45     297      74     216       6    1088

  Fragments arranged by size: 

                1088     545     297     216      74      45       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     943    2271 
   Size:        943    1328
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1753    2271 
   Size:       1753     518
 
EcoRI G'AATT_C

Cuts at:      0      81    1491    1804    2271 
   Size:         81    1410     313     467

  Fragments arranged by size: 

                1410     467     313      81
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     163     859     900    1332    1644    2137    2271 
   Size:        163     696      41     432     312     493     134

  Fragments arranged by size: 

                 696     493     432     312     163     134      41
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     466     498     609     641     811     843     865     897 
   Size:        466      32     111      32     170      32      22      32

Cuts at:    897    2271 
   Size:       1374

  Fragments arranged by size: 

                1374     466     170     111      32      32      32      32
                  22
 
HincII GTy'rAC

Cuts at:      0     730     796    1127    2271 
   Size:        730      66     331    1144

  Fragments arranged by size: 

                1144     730     331      66
 
MfeI C'AATT_G

Cuts at:      0     245    2271 
   Size:        245    2026
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     835    2153    2271 
   Size:         19     816    1318     118

  Fragments arranged by size: 

                1318     816     118      19
 
MscI TGG'CCA

Cuts at:      0     695    2271 
   Size:        695    1576
 
MslI CAynn'nnrTG

Cuts at:      0     701     755    1939    2271 
   Size:        701      54    1184     332

  Fragments arranged by size: 

                1184     701     332      54
 
MspA1I CmG'CkG

Cuts at:      0     104    1675    2271 
   Size:        104    1571     596

  Fragments arranged by size: 

                1571     596     104
 
NdeI CA'TA_TG

Cuts at:      0    2223    2271 
   Size:       2223      48
 
NspI r_CATG'y

Cuts at:      0    1992    2271 
   Size:       1992     279
 
PciI A'CATG_T

Cuts at:      0    1988    2271 
   Size:       1988     283
 
PshAI GACnn'nnGTC

Cuts at:      0    2232    2271 
   Size:       2232      39
 
PsiI TTA'TAA

Cuts at:      0     824    2271 
   Size:        824    1447
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     251     283    2271 
   Size:        251      32    1988

  Fragments arranged by size: 

                1988     251      32
 
PvuII CAG'CTG

Cuts at:      0     104    1675    2271 
   Size:        104    1571     596

  Fragments arranged by size: 

                1571     596     104
 
SapI GCTCTTCn'nnn_

Cuts at:      0     417    2092    2271 
   Size:        417    1675     179

  Fragments arranged by size: 

                1675     417     179
 
ScaI AGT'ACT

Cuts at:      0    1032    2271 
   Size:       1032    1239
 
SfcI C'TryA_G

Cuts at:      0     911    1730    2271 
   Size:        911     819     541

  Fragments arranged by size: 

                 911     819     541
 
SmlI C'TyrA_G

Cuts at:      0     325    1060    2271 
   Size:        325     735    1211

  Fragments arranged by size: 

                1211     735     325
 
SpeI A'CTAG_T

Cuts at:      0    1170    2271 
   Size:       1170    1101
 
SspI AAT'ATT

Cuts at:      0    1010    2271 
   Size:       1010    1261
 
StyI C'CwwG_G

Cuts at:      0    1499    2271 
   Size:       1499     772
 
TatI w'GTAC_w

Cuts at:      0    1030    2271 
   Size:       1030    1241
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2197    2229    2271 
   Size:       2197      32      42

  Fragments arranged by size: 

                2197      42      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1207    2271 
   Size:       1207    1064
 
XmnI GAAnn'nnTTC

Cuts at:      0    1570    2086    2271 
   Size:       1570     516     185

  Fragments arranged by size: 

                1570     516     185
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AleI    AlwNI     ApoI     BanI
    BanII     BbsI    BciVI     BclI     BglI     BmrI     BpmI    BpuEI
     BsaI    BsaBI    BsaHI    BsaXI    BseRI  BsiHKAI Bsp1286I    BspHI
    BsrDI    BsrFI    BstBI    BstXI  BstZ17I     DraI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I   HincII     MfeI
     MmeI     MscI     MslI   MspA1I     NdeI     NspI     PciI    PshAI
     PsiI     PsrI    PvuII     SapI     ScaI     SfcI     SmlI     SpeI
     SspI     StyI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaWI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MluI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI   PspOMI    PspXI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     StuI     SwaI
    TaqII  Tth111I     XbaI     XhoI     XmaI     ZraI
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