Restriction map for pF1KA1216
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      ApaI        ApaLI       
AscI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BbeI        BbrPI       BbvCI       
BcnI        BfrI        BglI        BlnI        BsaAI       
BseAI       BsiWI       BsoBI       BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrFI       BssHII      
BstAPI      BstEII      BstPI       Cfr10I      ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoO65I     EcoRV       FauI        
FseI        FspI        HindIII     HpaI        KasI        
KpnI        KspI        MluI        MroI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII        3531  GACGTC               GACGT^C
AccI         3742  GTATAC               GT^[AC][GT]AC
AcyI         3531  GACGTC               G[GA]^CG[CT]C
AocI         2245  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       884  AGCGCT               AGC^GCT
BalI         2606  TGGCCA               TGG^CCA
BclI         3452  TGATCA               T^GATCA
BglII         405  AGATCT               A^GATCT
BlpI          245  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      245  GCTTAGC              GC^T[ACGT]AGC
BsaBI         775  GATAAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        3531  GACGTC               G[GA]^CG[CT]C
BsgI         3025  CTGCAC               GTGCAG(16/14)
BsiEI        3112  CGACCG               CG[GA][CT]^CG
BsmBI           4  GAGACG               CGTCTC(1/5)
BsmFI        3254  GTCCC                GGGAC(10/14)
BssSI        3164  CACGAG               CACGAG(-5/-1)
Bst1107I     3742  GTATAC               GTA^TAC
BstBI          25  TTCGAA               TT^CGAA
BstZ17I      3742  GTATAC               GTA^TAC
Bsu36I       2245  CCTCAGG              CC^T[ACGT]AGG
CelII         245  GCTTAGC              GC^T[ACGT]AGC
EaeI         2606  TGGCCA               [CT]^GGCC[GA]
Eco47III      884  AGCGCT               AGC^GCT
Eco81I       2245  CCTCAGG              CC^T[ACGT]AGG
EcoNI         751  CCTTTGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        3724  GAATTC               G^AATTC
FbaI         3452  TGATCA               T^GATCA
HaeII         884  AGCGCT               [GA]GCGC^[CT]
HgaI            6  GACGC                GACGC(5/10)
Hin1I        3531  GACGTC               G[GA]^CG[CT]C
MamI          775  GATAAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3443  CAATTG               C^AATTG
MluNI        2606  TGGCCA               TGG^CCA
MscI         2606  TGGCCA               TGG^CCA
MunI         3443  CAATTG               C^AATTG
NspV           25  TTCGAA               TT^CGAA
PflMI         906  CCAGTTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI         1191  GCTCTTC              GCTCTTC(1/4)
ScaI         3699  AGTACT               AGT^ACT
SfuI           25  TTCGAA               TT^CGAA
SpeI         3222  ACTAGT               A^CTAGT
StuI          759  AGGCCT               AGG^CCT
SwaI         3860  ATTTAAAT             ATTT^AAAT
Van91I        906  CCAGTTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII        2811  CGCG                 CG^CG
             3055  CGCG                
AseI         2407  ATTAAT               AT^TAAT
             2592  ATTAAT              
AsnI         2407  ATTAAT               AT^TAAT
             2592  ATTAAT              
Asp700I      1188  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3697  GAAGTACTTC          
BcgI         1935  CGAGAGCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3218  GCAGACTAGTCG        
BsaWI        1829  ACCGGA               [AT]^CCGG[AT]
             2934  TCCGGT              
Bsp1407I      468  TGTACA               T^GTACA
             2832  TGTACA              
BsrGI         468  TGTACA               T^GTACA
             2832  TGTACA              
BstF5I       1832  GGATG                GGATG(2/0)
             3434  GGATG               
BstUI        2811  CGCG                 CG^CG
             3055  CGCG                
DsaI          932  CCATGG               C^C[GA][CT]GG
             1472  CCATGG              
FokI         1832  GGATG                GGATG(9/13)
             3434  GGATG               
HapII        1830  CCGG                 C^CGG
             2935  CCGG                
HincII       1254  GTCAAC               GT[CT]^[GA]AC
             3841  GTTGAC              
HindII       1254  GTCAAC               GT[CT]^[GA]AC
             3841  GTTGAC              
HpaII        1830  CCGG                 C^CGG
             2935  CCGG                
MaeII        3532  ACGT                 A^CGT
             3622  ACGT                
MspA1I       3299  CAGCTG               C[AC]G^C[GT]G
             4019  CAGCTG              
MspI         1830  CCGG                 C^CGG
             2935  CCGG                
MvnI         2811  CGCG                 CG^CG
             3055  CGCG                
NcoI          932  CCATGG               C^CATGG
             1472  CCATGG              
NdeI          770  CATATG               CA^TATG
             1051  CATATG              
PshBI        2407  ATTAAT               AT^TAAT
             2592  ATTAAT              
PvuII        3299  CAGCTG               CAG^CTG
             4019  CAGCTG              
SspBI         468  TGTACA               T^GTACA
             2832  TGTACA              
XmnI         1188  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3697  GAAGTACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BamHI       
BanI        BanII       BbsI        BbvI        BciVI       
BfaI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmI        Bsp1286I    BspHI       BsrDI       
BsrI        BssKI       BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       EarI        EcoO109I    EcoRII      
EcoT14I     EcoT22I     Fnu4HI      HaeIII      HhaI        
HinP1I      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PleI        PstI        RcaI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com