Restriction map for pF1KA1216
 (Linear) (Six-base) MAPSORT of: KIAA1216.seq  Check: 3115  from: 1  to: 4104



KIAA1216.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AatII G_ACGT'C

Cuts at:      0    3535    4104 
   Size:       3535     569
 
AccI GT'mk_AC

Cuts at:      0    3743    4104 
   Size:       3743     361
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     114     596     618    1532    2060    2131    3121 
   Size:        110       4     482      22     914     528      71     990

Cuts at:   3121    3716    4104 
   Size:        595     388

  Fragments arranged by size: 

                 990     914     595     528     482     388     110      71
                  22       4
 
AfeI AGC'GCT

Cuts at:      0     886    4104 
   Size:        886    3218
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     161     193    4104 
   Size:        161      32    3911

  Fragments arranged by size: 

                3911     161      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     480     553    1234    1869    2038    2110    2664    4104 
   Size:        480      73     681     635     169      72     554    1440

  Fragments arranged by size: 

                1440     681     635     554     480     169      73      72
            
 
ApoI r'AATT_y

Cuts at:      0     224    1449    2543    2718    2986    3724    4104 
   Size:        224    1225    1094     175     268     738     380

  Fragments arranged by size: 

                1225    1094     738     380     268     224     175
 
AseI AT'TA_AT

Cuts at:      0    2408    2593    4104 
   Size:       2408     185    1511

  Fragments arranged by size: 

                2408    1511     185
 
BamHI G'GATC_C

Cuts at:      0     729    2823    3873    4104 
   Size:        729    2094    1050     231

  Fragments arranged by size: 

                2094    1050     729     231
 
BanI G'GyrC_C

Cuts at:      0     703    3647    4077    4104 
   Size:        703    2944     430      27

  Fragments arranged by size: 

                2944     703     430      27
 
BanII G_rGCy'C

Cuts at:      0     460    1482    2353    3335    4104 
   Size:        460    1022     871     982     769

  Fragments arranged by size: 

                1022     982     871     769     460
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     670    2365    2872    3724    3976    4104 
   Size:        670    1695     507     852     252     128

  Fragments arranged by size: 

                1695     852     670     507     252     128
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1924    1958    3207    3241    4104 
   Size:       1924      34    1249      34     863

  Fragments arranged by size: 

                1924    1249     863      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      75     187     581     621    1511    2949    4104 
   Size:         75     112     394      40     890    1438    1155

  Fragments arranged by size: 

                1438    1155     890     394     112      75      40
 
BclI T'GATC_A

Cuts at:      0    3452    4104 
   Size:       3452     652
 
BfrBI ATG'CAT

Cuts at:      0    1438    1789    1836    2537    4104 
   Size:       1438     351      47     701    1567

  Fragments arranged by size: 

                1567    1438     701     351      47
 
BglII A'GATC_T

Cuts at:      0     405    4104 
   Size:        405    3699
 
BlpI GC'TnA_GC

Cuts at:      0     246    4104 
   Size:        246    3858
 
Bme1580I G_kGCm'C

Cuts at:      0    4082    4104 
   Size:       4082      22
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1222    1245    4104 
   Size:       1222      23    2859

  Fragments arranged by size: 

                2859    1222      23
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1538    1882    1961    2183    4104 
   Size:       1538     344      79     222    1921

  Fragments arranged by size: 

                1921    1538     344     222      79
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     919    1664    2971    3294    4040    4104 
   Size:        919     745    1307     323     746      64

  Fragments arranged by size: 

                1307     919     746     745     323      64
 
Bpu10I CC'TnA_GC

Cuts at:      0    2194    4104 
   Size:       2194    1910
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     987    1834    4104 
   Size:        340     647     847    2270

  Fragments arranged by size: 

                2270     847     647     340
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     274     940    3044    4058    4104 
   Size:        274     666    2104    1014      46

  Fragments arranged by size: 

                2104    1014     666     274      46
 
BsaBI GATnn'nnATC

Cuts at:      0     779    4104 
   Size:        779    3325
 
BsaHI Gr'CG_yC

Cuts at:      0    3532    4104 
   Size:       3532     572
 
BsaWI w'CCGG_w

Cuts at:      0    1829    2934    4104 
   Size:       1829    1105    1170

  Fragments arranged by size: 

                1829    1170    1105
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     892     922    1469    1499    1793    1823    2272    2302 
   Size:        892      30     547      30     294      30     449      30

Cuts at:   2302    4104 
   Size:       1802

  Fragments arranged by size: 

                1802     892     547     449     294      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     967    2159    2296    2299    2348    4104 
   Size:        967    1192     137       3      49    1756

  Fragments arranged by size: 

                1756    1192     967     137      49       3
 
BseYI C'CCAG_C

Cuts at:      0    3297    3523    4104 
   Size:       3297     226     581

  Fragments arranged by size: 

                3297     581     226
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3010    4104 
   Size:       3010    1094
 
BsiEI CG_ry'CG

Cuts at:      0    3115    4104 
   Size:       3115     989
 
BsiHKAI G_wGCw'C

Cuts at:      0     460    1482    1942    2027    2732    3335    4104 
   Size:        460    1022     460      85     705     603     769

  Fragments arranged by size: 

                1022     769     705     603     460     460      85
 
BsmI GAATG_Cn'

Cuts at:      0    2221    3165    3375    3444    4104 
   Size:       2221     944     210      69     660

  Fragments arranged by size: 

                2221     944     660     210      69
 
Bsp1286I G_dGCh'C

Cuts at:      0     460    1482    1942    2027    2353    2732    3335    4082 
   Size:        460    1022     460      85     326     379     603     747

Cuts at:   4082    4104 
   Size:         22

  Fragments arranged by size: 

                1022     747     603     460     460     379     326      85
                  22
 
BspHI T'CATG_A

Cuts at:      0     165    2156    2991    3492    4104 
   Size:        165    1991     835     501     612

  Fragments arranged by size: 

                1991     835     612     501     165
 
BsrDI GCAATG_nn'

Cuts at:      0     257    1277    1625    4104 
   Size:        257    1020     348    2479

  Fragments arranged by size: 

                2479    1020     348     257
 
BsrGI T'GTAC_A

Cuts at:      0     468    2832    4104 
   Size:        468    2364    1272

  Fragments arranged by size: 

                2364    1272     468
 
BssSI C'ACGA_G

Cuts at:      0    3164    4104 
   Size:       3164     940
 
BstBI TT'CG_AA

Cuts at:      0      26    4104 
   Size:         26    4078
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     843    2454    2732    2949    3754    4104 
   Size:        843    1611     278     217     805     350

  Fragments arranged by size: 

                1611     843     805     350     278     217
 
BstYI r'GATC_y

Cuts at:      0     405     729     780     915    1552    1796    2823    2837 
   Size:        405     324      51     135     637     244    1027      14

Cuts at:   2837    3873    4104 
   Size:       1036     231

  Fragments arranged by size: 

                1036    1027     637     405     324     244     231     135
                  51      14
 
BstZ17I GTA'TAC

Cuts at:      0    3744    4104 
   Size:       3744     360
 
Bsu36I CC'TnA_GG

Cuts at:      0    2246    4104 
   Size:       2246    1858
 
BtgI C'CryG_G

Cuts at:      0     932    1472    4104 
   Size:        932     540    2632

  Fragments arranged by size: 

                2632     932     540
 
BtsI GCAGTG_nn'

Cuts at:      0    1940    3458    3578    4031    4104 
   Size:       1940    1518     120     453      73

  Fragments arranged by size: 

                1940    1518     453     120      73
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     970    1005    4104 
   Size:        970      35    3099

  Fragments arranged by size: 

                3099     970      35
 
DraI TTT'AAA

Cuts at:      0     357     866    3863    3974    4104 
   Size:        357     509    2997     111     130

  Fragments arranged by size: 

                2997     509     357     130     111
 
EaeI y'GGCC_r

Cuts at:      0    2606    4104 
   Size:       2606    1498
 
EarI CTCTTCn'nnn_

Cuts at:      0      23      50     746    1070    1198    4104 
   Size:         23      27     696     324     128    2906

  Fragments arranged by size: 

                2906     696     324     128      27      23
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3553    4104 
   Size:       3553     551
 
EcoICRI GAG'CTC

Cuts at:      0     458    1480    3333    4104 
   Size:        458    1022    1853     771

  Fragments arranged by size: 

                1853    1022     771     458
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     114     596     618     919    1532    1664    2060 
   Size:        110       4     482      22     301     613     132     396

Cuts at:   2060    2131    2971    3121    3294    3716    4040    4104 
   Size:         71     840     150     173     422     324      64

  Fragments arranged by size: 

                 840     613     482     422     396     324     301     173
                 150     132     110      71      64      22       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     755    4104 
   Size:        755    3349
 
EcoO109I rG'GnC_Cy

Cuts at:      0     125    2450    2805    4104 
   Size:        125    2325     355    1299

  Fragments arranged by size: 

                2325    1299     355     125
 
EcoRI G'AATT_C

Cuts at:      0    3724    4104 
   Size:       3724     380
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     245     601    2161    3155    3419    3721    4104 
   Size:        245     356    1560     994     264     302     383

  Fragments arranged by size: 

                1560     994     383     356     302     264     245
 
HaeII r_GCGC'y

Cuts at:      0     888    4104 
   Size:        888    3216
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2582    2614    2829    2861    4104 
   Size:       2582      32     215      32    1243

  Fragments arranged by size: 

                2582    1243     215      32      32
 
HincII GTy'rAC

Cuts at:      0    1256    3843    4104 
   Size:       1256    2587     261

  Fragments arranged by size: 

                2587    1256     261
 
MfeI C'AATT_G

Cuts at:      0    3443    4104 
   Size:       3443     661
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3735    4104 
   Size:       3735     369
 
MscI TGG'CCA

Cuts at:      0    2608    4104 
   Size:       2608    1496
 
MslI CAynn'nnrTG

Cuts at:      0      76    1788    1928    3032    4104 
   Size:         76    1712     140    1104    1072

  Fragments arranged by size: 

                1712    1104    1072     140      76
 
MspA1I CmG'CkG

Cuts at:      0    3301    4021    4104 
   Size:       3301     720      83

  Fragments arranged by size: 

                3301     720      83
 
NcoI C'CATG_G

Cuts at:      0     932    1472    4104 
   Size:        932     540    2632

  Fragments arranged by size: 

                2632     932     540
 
NdeI CA'TA_TG

Cuts at:      0     771    1052    4104 
   Size:        771     281    3052

  Fragments arranged by size: 

                3052     771     281
 
NsiI A_TGCA'T

Cuts at:      0    1440    1791    1838    2539    4104 
   Size:       1440     351      47     701    1565

  Fragments arranged by size: 

                1565    1440     701     351      47
 
NspI r_CATG'y

Cuts at:      0      75    1789    3640    3831    4104 
   Size:         75    1714    1851     191     273

  Fragments arranged by size: 

                1851    1714     273     191      75
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     912    4104 
   Size:        912    3192
 
PfoI T'CCnGG_A

Cuts at:      0    1580    4104 
   Size:       1580    2524
 
PsiI TTA'TAA

Cuts at:      0    1957    4104 
   Size:       1957    2147
 
PstI C_TGCA'G

Cuts at:      0      17    1376    1896    4026    4104 
   Size:         17    1359     520    2130      78

  Fragments arranged by size: 

                2130    1359     520      78      17
 
PvuII CAG'CTG

Cuts at:      0    3301    4021    4104 
   Size:       3301     720      83

  Fragments arranged by size: 

                3301     720      83
 
SacI G_AGCT'C

Cuts at:      0     460    1482    3335    4104 
   Size:        460    1022    1853     769

  Fragments arranged by size: 

                1853    1022     769     460
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1198    4104 
   Size:       1198    2906
 
ScaI AGT'ACT

Cuts at:      0    3701    4104 
   Size:       3701     403
 
SfcI C'TryA_G

Cuts at:      0      13     672    1327    1372    1892    2215    2421    3710 
   Size:         13     659     655      45     520     323     206    1289

Cuts at:   3710    4022    4104 
   Size:        312      82

  Fragments arranged by size: 

                1289     659     655     520     323     312     206      82
                  45      13
 
SmlI C'TyrA_G

Cuts at:      0     319    1002    1813    4104 
   Size:        319     683     811    2291

  Fragments arranged by size: 

                2291     811     683     319
 
SpeI A'CTAG_T

Cuts at:      0    3222    4104 
   Size:       3222     882
 
StuI AGG'CCT

Cuts at:      0     761    4104 
   Size:        761    3343
 
StyI C'CwwG_G

Cuts at:      0     932    1472    1673    2069    2453    3178    3341    4104 
   Size:        932     540     201     396     384     725     163     763

  Fragments arranged by size: 

                 932     763     725     540     396     384     201     163
            
 
SwaI ATTT'AAAT

Cuts at:      0    3863    4104 
   Size:       3863     241
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     294     964    3736    3871    4104 
   Size:        294     670    2772     135     233

  Fragments arranged by size: 

                2772     670     294     233     135
 
TatI w'GTAC_w

Cuts at:      0     468    2832    3699    4104 
   Size:        468    2364     867     405

  Fragments arranged by size: 

                2364     867     468     405
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1479    1671    3088    3269    3476    4104 
   Size:       1479     192    1417     181     207     628

  Fragments arranged by size: 

                1479    1417     628     207     192     181
 
XmnI GAAnn'nnTTC

Cuts at:      0    1192    3701    4104 
   Size:       1192    2509     403

  Fragments arranged by size: 

                2509    1192     403
 
ZraI GAC'GTC

Cuts at:      0    3533    4104 
   Size:       3533     571
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI     AloI    AlwNI     ApoI     AseI
    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI    BsrGI    BssSI
    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI    CspCI
     DraI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    HaeII    Hin4I   HincII     MfeI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NsiI     NspI    PflMI     PfoI
     PsiI     PstI    PvuII     SacI     SapI     ScaI     SfcI     SmlI
     SpeI     StuI     StyI     SwaI    TaqII     TatI     XcmI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI
     BbeI    BbvCI     BglI    BmgBI     BmtI    BsaAI    BsiWI    BsmBI
    BspEI    BspMI    BsrBI    BsrFI   BssHII   BstAPI   BstEII    BtgZI
     ClaI   DraIII     DrdI     EagI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MluI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     TstI  Tth111I
     XbaI     XhoI     XmaI
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