Restriction map for pF1KA1182
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        AhdI        AocI        ApaI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvrII       BamHI       BbrPI       BciVI       
BclI        BfaI        BfrI        BglI        BlnI        
BsaAI       BsaBI       BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspLU11I    BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HinfI       HpaI        KpnI        MaeI        MamI        
MfeI        MluI        MseI        MslI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        ScaI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tth111I     Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccIII        369  TCCGGA               T^CCGGA
AcsI         1423  GAATTT               [GA]^AATT[CT]
Aor51HI       520  AGCGCT               AGC^GCT
ApoI         1423  GAATTT               [GA]^AATT[CT]
Asp700I       280  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1336  CCCGGG               C^[CT]CG[GA]G
BalI           29  TGGCCA               TGG^CCA
BbeI          584  GGCGCC               GGCGC^C
BglII         443  AGATCT               A^GATCT
BlpI          715  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      715  GCTGAGC              GC^T[ACGT]AGC
BseAI         369  TCCGGA               T^CCGGA
BsmBI        1687  GAGACG               CGTCTC(1/5)
BsoBI        1336  CCCGGG               C^[CT]CG[GA]G
BspEI         369  TCCGGA               T^CCGGA
BspHI         347  TCATGA               T^CATGA
BstYI         443  AGATCT               [GA]^GATC[CT]
CelII         715  GCTGAGC              GC^T[ACGT]AGC
DraIII        786  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1066  GACAGCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      520  AGCGCT               AGC^GCT
HindIII      1227  AAGCTT               A^AGCTT
KasI          584  GGCGCC               G^GCGCC
KspI          169  CCGCGG               CCGC^GG
MaeIII        917  GTGAC                ^GT[ACGT]AC
MflI          443  AGATCT               [GA]^GATC[CT]
MluNI          29  TGGCCA               TGG^CCA
MroI          369  TCCGGA               T^CCGGA
MscI           29  TGGCCA               TGG^CCA
NaeI         1692  GCCGGC               GCC^GGC
NarI          584  GGCGCC               GG^CGCC
NgoMIV       1692  GCCGGC               G^CCGGC
PvuII        1648  CAGCTG               CAG^CTG
RcaI          347  TCATGA               T^CATGA
SacII         169  CCGCGG               CCGC^GG
SbfI          963  CCTGCAGG             CCTGCA^GG
SfoI          584  GGCGCC               GGC^GCC
SmaI         1336  CCCGGG               CCC^GGG
Sse8387I      963  CCTGCAGG             CCTGCA^GG
Tsp45I        917  GTGAC                ^GT[GC]AC
Tsp509I      1424  AATT                 ^AATT
XhoII         443  AGATCT               [GA]^GATC[CT]
XmaCI        1336  CCCGGG               C^CCGGG
XmaI         1336  CCCGGG               C^CCGGG
XmnI          280  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Alw44I        694  GTGCAC               G^TGCAC
             1498  GTGCAC              
ApaLI         694  GTGCAC               G^TGCAC
             1498  GTGCAC              
AviII         665  TGCGCA               TGC^GCA
              680  TGCGCA              
BbsI          280  GAAGAC               GAAGAC(2/6)
             1075  GTCTTC              
BbvCI         256  GCTGAGG              CCTCAGC(-5/-2)
             1183  GCTGAGG             
BcgI         1419  CGACGAATTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1448  GCAGCCTCTTCG        
BpuAI         280  GAAGAC               GAAGAC(2/6)
             1075  GTCTTC              
BsaI           52  GAGACC               GGTCTC(1/5)
             1348  GAGACC              
BsaWI         369  TCCGGA               [AT]^CCGG[AT]
             1658  ACCGGA              
BsrFI        1502  ACCGGC               [GA]^CCGG[CT]
             1692  GCCGGC              
BsrI          186  CCAGT                ACTGG(1/-1)
             1198  CCAGT               
Cfr10I       1502  ACCGGC               [GA]^CCGG[CT]
             1692  GCCGGC              
EcoT14I       362  CCAAGG               C^C[AT][AT]GG
             1483  CCAAGG              
FspI          665  TGCGCA               TGC^GCA
              680  TGCGCA              
SacI          538  GAGCTC               GAGCT^C
             1102  GAGCTC              
SapI          294  GAAGAGC              GCTCTTC(1/4)
              672  GCTCTTC             
SexAI         787  ACCTGGT              A^CC[AT]GGT
             1111  ACCAGGT             
SmlI           79  CTCAAG               C^T[CT][GA]AG
             1432  CTTGAG              
StyI          362  CCAAGG               C^C[AT][AT]GG
             1483  CCAAGG              
XcmI           99  CCAGACAGAGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              929  CCATCAACACGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrBI       BssKI       BstAPI      BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        Ksp632I     MaeII       MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PpuMI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TseI        TspRI       TthHB8I     
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