Restriction map for pF1KA1182
 (Linear) (Six-base) MAPSORT of: KIAA1182.seq  Check: 1822  from: 1  to: 1740



KIAA1182.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     365     876     971    1397    1538    1740 
   Size:        365     511      95     426     141     202

  Fragments arranged by size: 

                 511     426     365     202     141      95
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     115     403     513     514    1740 
   Size:         67      48     288     110       1    1226

  Fragments arranged by size: 

                1226     288     110      67      48       1
 
AfeI AGC'GCT

Cuts at:      0     522    1740 
   Size:        522    1218
 
AlwNI CAG_nnn'CTG

Cuts at:      0      21     609     663     891     972    1740 
   Size:         21     588      54     228      81     768

  Fragments arranged by size: 

                 768     588     228      81      54      21
 
ApaLI G'TGCA_C

Cuts at:      0     694    1498    1740 
   Size:        694     804     242

  Fragments arranged by size: 

                 804     694     242
 
ApoI r'AATT_y

Cuts at:      0    1423    1740 
   Size:       1423     317
 
AvaI C'yCGr_G

Cuts at:      0    1336    1740 
   Size:       1336     404
 
BanI G'GyrC_C

Cuts at:      0     584     791     959    1740 
   Size:        584     207     168     781

  Fragments arranged by size: 

                 781     584     207     168
 
BanII G_rGCy'C

Cuts at:      0     542    1088    1106    1740 
   Size:        542     546      18     634

  Fragments arranged by size: 

                 634     546     542      18
 
BbeI G_GCGC'C

Cuts at:      0     588    1740 
   Size:        588    1152
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     287    1068    1740 
   Size:        287     781     672

  Fragments arranged by size: 

                 781     672     287
 
BbvCI CC'TCA_GC

Cuts at:      0     257    1184    1740 
   Size:        257     927     556

  Fragments arranged by size: 

                 927     556     257
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1408    1437    1442    1471    1740 
   Size:       1408      29       5      29     269

  Fragments arranged by size: 

                1408     269      29      29       5
 
BglII A'GATC_T

Cuts at:      0     443    1740 
   Size:        443    1297
 
BlpI GC'TnA_GC

Cuts at:      0     716    1740 
   Size:        716    1024
 
Bme1580I G_kGCm'C

Cuts at:      0     698    1502    1724    1740 
   Size:        698     804     222      16

  Fragments arranged by size: 

                 804     698     222      16
 
BmgBI CAC'GTC

Cuts at:      0     747    1740 
   Size:        747     993
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1192    1740 
   Size:       1192     548
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     320    1638    1740 
   Size:        320    1318     102

  Fragments arranged by size: 

                1318     320     102
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     241     310     529    1579    1600    1720    1740 
   Size:        196      45      69     219    1050      21     120      20

  Fragments arranged by size: 

                1050     219     196     120      69      45      21      20
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     257    1184    1740 
   Size:        257     927     556

  Fragments arranged by size: 

                 927     556     257
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64    1453    1740 
   Size:         64    1389     287

  Fragments arranged by size: 

                1389     287      64
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      46    1342    1740 
   Size:         46    1296     398

  Fragments arranged by size: 

                1296     398      46
 
BsaHI Gr'CG_yC

Cuts at:      0     166     585     800     866    1690    1740 
   Size:        166     419     215      66     824      50

  Fragments arranged by size: 

                 824     419     215     166      66      50
 
BsaWI w'CCGG_w

Cuts at:      0     369    1658    1740 
   Size:        369    1289      82

  Fragments arranged by size: 

                1289     369      82
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     214     244    1464    1494    1592    1622    1740 
   Size:        214      30    1220      30      98      30     118

  Fragments arranged by size: 

                1220     214     118      98      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1201    1312    1510    1740 
   Size:       1201     111     198     230

  Fragments arranged by size: 

                1201     230     198     111
 
BseYI C'CCAG_C

Cuts at:      0     891    1038    1625    1666    1714    1740 
   Size:        891     147     587      41      48      26

  Fragments arranged by size: 

                 891     587     147      48      41      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     142     535     622     687     718     744    1273 
   Size:         51      91     393      87      65      31      26     529

Cuts at:   1273    1516    1740 
   Size:        243     224

  Fragments arranged by size: 

                 529     393     243     224      91      87      65      51
                  31      26
 
BsiHKAI G_wGCw'C

Cuts at:      0     542     698     952    1106    1502    1740 
   Size:        542     156     254     154     396     238

  Fragments arranged by size: 

                 542     396     254     238     156     154
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1681    1740 
   Size:       1681      59
 
Bsp1286I G_dGCh'C

Cuts at:      0     542     698     952    1088    1106    1502    1724    1740 
   Size:        542     156     254     136      18     396     222      16

  Fragments arranged by size: 

                 542     396     254     222     156     136      18      16
            
 
BspEI T'CCGG_A

Cuts at:      0     369    1740 
   Size:        369    1371
 
BspHI T'CATG_A

Cuts at:      0     347    1740 
   Size:        347    1393
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     164     365     876     971    1397    1538    1740 
   Size:        164     201     511      95     426     141     202

  Fragments arranged by size: 

                 511     426     202     201     164     141      95
 
BsrBI CCG'CTC

Cuts at:      0      78     816    1598    1737    1740 
   Size:         78     738     782     139       3

  Fragments arranged by size: 

                 782     738     139      78       3
 
BsrFI r'CCGG_y

Cuts at:      0    1502    1692    1740 
   Size:       1502     190      48

  Fragments arranged by size: 

                1502     190      48
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     663    1262    1512    1681    1740 
   Size:        663     599     250     169      59

  Fragments arranged by size: 

                 663     599     250     169      59
 
BstYI r'GATC_y

Cuts at:      0     443    1740 
   Size:        443    1297
 
BtgI C'CryG_G

Cuts at:      0     169     332     425     764    1740 
   Size:        169     163      93     339     976

  Fragments arranged by size: 

                 976     339     169     163      93
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     222    1740 
   Size:        222    1518
 
BtsI GCAGTG_nn'

Cuts at:      0     988    1613    1740 
   Size:        988     625     127

  Fragments arranged by size: 

                 988     625     127
 
DraIII CAC_nnn'GTG

Cuts at:      0     791    1740 
   Size:        791     949
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1072    1740 
   Size:       1072     668
 
EaeI y'GGCC_r

Cuts at:      0      29     978    1415    1740 
   Size:         29     949     437     325

  Fragments arranged by size: 

                 949     437     325      29
 
EarI CTCTTCn'nnn_

Cuts at:      0     134     289     472     532     679    1459    1740 
   Size:        134     155     183      60     147     780     281

  Fragments arranged by size: 

                 780     281     183     155     147     134      60
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1315    1740 
   Size:       1315     425
 
EcoICRI GAG'CTC

Cuts at:      0     540    1104    1740 
   Size:        540     564     636

  Fragments arranged by size: 

                 636     564     540
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     115     196     241     310     403     513     514 
   Size:         67      48      81      45      69      93     110       1

Cuts at:    514     529    1579    1600    1720    1740 
   Size:         15    1050      21     120      20

  Fragments arranged by size: 

                1050     120     110      93      81      69      67      48
                  45      21      20      15       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     361     511    1482    1740 
   Size:        361     150     971     258

  Fragments arranged by size: 

                 971     361     258     150
 
EcoO109I rG'GnC_Cy

Cuts at:      0      18      42     462     897    1575    1740 
   Size:         18      24     420     435     678     165

  Fragments arranged by size: 

                 678     435     420     165      24      18
 
FspI TGC'GCA

Cuts at:      0     667     682    1740 
   Size:        667      15    1058

  Fragments arranged by size: 

                1058     667      15
 
HaeII r_GCGC'y

Cuts at:      0     524     588    1039    1740 
   Size:        524      64     451     701

  Fragments arranged by size: 

                 701     524     451      64
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     214     246    1740 
   Size:        214      32    1494

  Fragments arranged by size: 

                1494     214      32
 
HindIII A'AGCT_T

Cuts at:      0    1227    1740 
   Size:       1227     513
 
KasI G'GCGC_C

Cuts at:      0     584    1740 
   Size:        584    1156
 
MscI TGG'CCA

Cuts at:      0      31    1740 
   Size:         31    1709
 
MspA1I CmG'CkG

Cuts at:      0     171     606    1650    1740 
   Size:        171     435    1044      90

  Fragments arranged by size: 

                1044     435     171      90
 
NaeI GCC'GGC

Cuts at:      0    1694    1740 
   Size:       1694      46
 
NarI GG'CG_CC

Cuts at:      0     585    1740 
   Size:        585    1155
 
NgoMIV G'CCGG_C

Cuts at:      0    1692    1740 
   Size:       1692      48
 
PasI CC'CwG_GG

Cuts at:      0     995    1538    1740 
   Size:        995     543     202

  Fragments arranged by size: 

                 995     543     202
 
PfoI T'CCnGG_A

Cuts at:      0     506    1740 
   Size:        506    1234
 
PpuMI rG'GwC_Cy

Cuts at:      0      18      42     897    1740 
   Size:         18      24     855     843

  Fragments arranged by size: 

                 855     843      24      18
 
PstI C_TGCA'G

Cuts at:      0      17     329     659     851     968    1247    1447    1740 
   Size:         17     312     330     192     117     279     200     293

  Fragments arranged by size: 

                 330     312     293     279     200     192     117      17
            
 
PvuII CAG'CTG

Cuts at:      0    1650    1740 
   Size:       1650      90
 
SacI G_AGCT'C

Cuts at:      0     542    1106    1740 
   Size:        542     564     634

  Fragments arranged by size: 

                 634     564     542
 
SacII CC_GC'GG

Cuts at:      0     172    1740 
   Size:        172    1568
 
SapI GCTCTTCn'nnn_

Cuts at:      0     289     679    1740 
   Size:        289     390    1061

  Fragments arranged by size: 

                1061     390     289
 
SbfI CC_TGCA'GG

Cuts at:      0     968    1740 
   Size:        968     772
 
SexAI A'CCwGG_T

Cuts at:      0     787    1111    1740 
   Size:        787     324     629

  Fragments arranged by size: 

                 787     629     324
 
SfcI C'TryA_G

Cuts at:      0      13     325     655     847     964    1243    1443    1586 
   Size:         13     312     330     192     117     279     200     143

Cuts at:   1586    1740 
   Size:        154

  Fragments arranged by size: 

                 330     312     279     200     192     154     143     117
                  13
 
SfoI GGC'GCC

Cuts at:      0     586    1740 
   Size:        586    1154
 
SmaI CCC'GGG

Cuts at:      0    1338    1740 
   Size:       1338     402
 
SmlI C'TyrA_G

Cuts at:      0      79    1432    1740 
   Size:         79    1353     308

  Fragments arranged by size: 

                1353     308      79
 
SrfI GCCC'GGGC

Cuts at:      0    1338    1740 
   Size:       1338     402
 
StyI C'CwwG_G

Cuts at:      0     362    1483    1740 
   Size:        362    1121     257

  Fragments arranged by size: 

                1121     362     257
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     739    1740 
   Size:        739    1001
 
TatI w'GTAC_w

Cuts at:      0     500     532    1277    1740 
   Size:        500      32     745     463

  Fragments arranged by size: 

                 745     500     463      32
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1464    1496    1592    1624    1740 
   Size:       1464      32      96      32     116

  Fragments arranged by size: 

                1464     116      96      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     106     936    1740 
   Size:        106     830     804

  Fragments arranged by size: 

                 830     804     106
 
XmaI C'CCGG_G

Cuts at:      0    1336    1740 
   Size:       1336     404
 
XmnI GAAnn'nnTTC

Cuts at:      0     284    1740 
   Size:        284    1456
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AlwNI    ApaLI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BstAPI    BstYI     BtgI
    BtgZI     BtsI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I  HindIII     KasI
     MscI   MspA1I     NaeI     NarI   NgoMIV     PasI     PfoI    PpuMI
     PstI    PvuII     SacI    SacII     SapI     SbfI    SexAI     SfcI
     SfoI     SmaI     SmlI     SrfI     StyI    TaqII     TatI     TstI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BciVI     BclI    BfrBI     BglI     BmtI    BsaAI
    BsaBI    BsiEI    BsiWI     BsmI    BsrDI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI
     EagI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII     HpaI
     KpnI     MfeI     MluI     MmeI     MslI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     ScaI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SspI     StuI     SwaI  Tth111I     XbaI     XhoI
     ZraI
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