Restriction map for pF1KA1145
 (Linear) (Six-base) MAPSORT of: KIAA1145.seq  Check: 7597  from: 1  to: 1338



KIAA1145.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     406     859     988    1338 
   Size:        139     267     453     129     350

  Fragments arranged by size: 

                 453     350     267     139     129
 
AfeI AGC'GCT

Cuts at:      0     633    1021    1338 
   Size:        633     388     317

  Fragments arranged by size: 

                 633     388     317
 
AflII C'TTAA_G

Cuts at:      0       9    1338 
   Size:          9    1329
 
AlwNI CAG_nnn'CTG

Cuts at:      0     882     978    1338 
   Size:        882      96     360

  Fragments arranged by size: 

                 882     360      96
 
ApoI r'AATT_y

Cuts at:      0     128     579    1338 
   Size:        128     451     759

  Fragments arranged by size: 

                 759     451     128
 
BanI G'GyrC_C

Cuts at:      0    1249    1338 
   Size:       1249      89
 
BanII G_rGCy'C

Cuts at:      0     361    1082    1338 
   Size:        361     721     256

  Fragments arranged by size: 

                 721     361     256
 
BbvCI CC'TCA_GC

Cuts at:      0     821    1338 
   Size:        821     517
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     298     332     547     581    1338 
   Size:        298      34     215      34     757

  Fragments arranged by size: 

                 757     298     215      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     795    1338 
   Size:        795     543
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1009    1338 
   Size:       1009     329
 
BlpI GC'TnA_GC

Cuts at:      0     812    1338 
   Size:        812     526
 
BmgBI CAC'GTC

Cuts at:      0     689    1338 
   Size:        689     649
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1302    1338 
   Size:       1302      36
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     799     934    1096    1338 
   Size:        799     135     162     242

  Fragments arranged by size: 

                 799     242     162     135
 
Bpu10I CC'TnA_GC

Cuts at:      0     821    1338 
   Size:        821     517
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     483    1338 
   Size:        483     855
 
BsaAI yAC'GTr

Cuts at:      0     420    1338 
   Size:        420     918
 
BsaBI GATnn'nnATC

Cuts at:      0     395    1338 
   Size:        395     943
 
BsaWI w'CCGG_w

Cuts at:      0     536    1338 
   Size:        536     802
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     604    1006    1338 
   Size:        604     402     332

  Fragments arranged by size: 

                 604     402     332
 
BseYI C'CCAG_C

Cuts at:      0     287    1338 
   Size:        287    1051
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     865    1338 
   Size:        865     473
 
BsiEI CG_ry'CG

Cuts at:      0     701    1338 
   Size:        701     637
 
BsiHKAI G_wGCw'C

Cuts at:      0     361    1082    1338 
   Size:        361     721     256

  Fragments arranged by size: 

                 721     361     256
 
BsmI GAATG_Cn'

Cuts at:      0    1128    1338 
   Size:       1128     210
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1218    1338 
   Size:       1218     120
 
Bsp1286I G_dGCh'C

Cuts at:      0     361    1082    1338 
   Size:        361     721     256

  Fragments arranged by size: 

                 721     361     256
 
BspEI T'CCGG_A

Cuts at:      0     536    1338 
   Size:        536     802
 
BspHI T'CATG_A

Cuts at:      0    1169    1338 
   Size:       1169     169
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     950    1338 
   Size:        950     388
 
BsrDI GCAATG_nn'

Cuts at:      0     441     563    1338 
   Size:        441     122     775

  Fragments arranged by size: 

                 775     441     122
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1104    1338 
   Size:       1104     234
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     724     747    1246    1338 
   Size:        724      23     499      92

  Fragments arranged by size: 

                 724     499      92      23
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     152     188     267     382    1185    1338 
   Size:        152      36      79     115     803     153

  Fragments arranged by size: 

                 803     153     152     115      79      36
 
BtsI GCAGTG_nn'

Cuts at:      0     104     669    1338 
   Size:        104     565     669

  Fragments arranged by size: 

                 669     565     104
 
EaeI y'GGCC_r

Cuts at:      0     698     980    1338 
   Size:        698     282     358

  Fragments arranged by size: 

                 698     358     282
 
EagI C'GGCC_G

Cuts at:      0     698    1338 
   Size:        698     640
 
EarI CTCTTCn'nnn_

Cuts at:      0    1222    1274    1338 
   Size:       1222      52      64

  Fragments arranged by size: 

                1222      64      52
 
EcoICRI GAG'CTC

Cuts at:      0     359    1080    1338 
   Size:        359     721     258

  Fragments arranged by size: 

                 721     359     258
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     406     799     859     934     988    1096    1338 
   Size:        139     267     393      60      75      54     108     242

  Fragments arranged by size: 

                 393     267     242     139     108      75      60      54
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     886     970    1338 
   Size:        886      84     368

  Fragments arranged by size: 

                 886     368      84
 
EcoO109I rG'GnC_Cy

Cuts at:      0     617    1338 
   Size:        617     721
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     264    1338 
   Size:        264    1074
 
HaeII r_GCGC'y

Cuts at:      0     557     635    1023    1338 
   Size:        557      78     388     315

  Fragments arranged by size: 

                 557     388     315      78
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     151     183    1215    1247    1338 
   Size:        151      32    1032      32      91

  Fragments arranged by size: 

                1032     151      91      32      32
 
MscI TGG'CCA

Cuts at:      0     982    1338 
   Size:        982     356
 
MslI CAynn'nnrTG

Cuts at:      0     950    1153    1183    1338 
   Size:        950     203      30     155

  Fragments arranged by size: 

                 950     203     155      30
 
MspA1I CmG'CkG

Cuts at:      0     291     816     924    1338 
   Size:        291     525     108     414

  Fragments arranged by size: 

                 525     414     291     108
 
NruI TCG'CGA

Cuts at:      0    1204    1338 
   Size:       1204     134
 
NspI r_CATG'y

Cuts at:      0     471    1338 
   Size:        471     867
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     447    1338 
   Size:        447     891
 
PfoI T'CCnGG_A

Cuts at:      0      68      80     776    1034    1338 
   Size:         68      12     696     258     304

  Fragments arranged by size: 

                 696     304     258      68      12
 
PmlI CAC'GTG

Cuts at:      0     420    1338 
   Size:        420     918
 
PstI C_TGCA'G

Cuts at:      0     111     296    1109    1338 
   Size:        111     185     813     229

  Fragments arranged by size: 

                 813     229     185     111
 
PvuII CAG'CTG

Cuts at:      0     291     816     924    1338 
   Size:        291     525     108     414

  Fragments arranged by size: 

                 525     414     291     108
 
SacI G_AGCT'C

Cuts at:      0     361    1082    1338 
   Size:        361     721     256

  Fragments arranged by size: 

                 721     361     256
 
SfcI C'TryA_G

Cuts at:      0     107     292    1105    1338 
   Size:        107     185     813     233

  Fragments arranged by size: 

                 813     233     185     107
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1009    1338 
   Size:       1009     329
 
SmlI C'TyrA_G

Cuts at:      0       9    1338 
   Size:          9    1329
 
SpeI A'CTAG_T

Cuts at:      0     201    1338 
   Size:        201    1137
 
SphI G_CATG'C

Cuts at:      0     471    1338 
   Size:        471     867
 
StuI AGG'CCT

Cuts at:      0    1012    1338 
   Size:       1012     326
 
StyI C'CwwG_G

Cuts at:      0    1043    1338 
   Size:       1043     295
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1233    1338 
   Size:       1233     105
 

 Enzymes that do cut:

     AcuI     AfeI    AflII    AlwNI     ApoI     BanI    BanII    BbvCI
     BcgI    BciVI     BglI     BlpI    BmgBI     BmrI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI
   BstAPI    BstXI    BtgZI     BtsI     EaeI     EagI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I     MscI     MslI
   MspA1I     NruI     NspI    PflMI     PfoI     PmlI     PstI    PvuII
     SacI     SfcI     SfiI     SmlI     SpeI     SphI     StuI     StyI
    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI     BclI    BfrBI
    BglII Bme1580I     BmtI     BplI    BpuEI    BsaHI    BsaXI    BsiWI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI
     EciI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NsiI     PacI     PasI
     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI
     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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