Restriction map for pF1KA1132
 (Linear) (Six-base) MAPSORT of: KIAA1132.seq  Check: 4157  from: 1  to: 6159



KIAA1132.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      90    5280    6159 
   Size:         90    5190     879

  Fragments arranged by size: 

                5190     879      90
 
AccI GT'mk_AC

Cuts at:      0    3149    6159 
   Size:       3149    3010
 
Acc65I G'GTAC_C

Cuts at:      0     176    6159 
   Size:        176    5983
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      74     434     991    1224    2575    2577    4425    4882 
   Size:         74     360     557     233    1351       2    1848     457

Cuts at:   4882    6159 
   Size:       1277

  Fragments arranged by size: 

                1848    1351    1277     557     457     360     233      74
                   2
 
AfeI AGC'GCT

Cuts at:      0    4312    6000    6159 
   Size:       4312    1688     159

  Fragments arranged by size: 

                4312    1688     159
 
AflIII A'CryG_T

Cuts at:      0    4082    5153    6159 
   Size:       4082    1071    1006

  Fragments arranged by size: 

                4082    1071    1006
 
AgeI A'CCGG_T

Cuts at:      0    1448    5225    6159 
   Size:       1448    3777     934

  Fragments arranged by size: 

                3777    1448     934
 
AleI CACnn'nnGTG

Cuts at:      0    1281    4933    6154    6159 
   Size:       1281    3652    1221       5

  Fragments arranged by size: 

                3652    1281    1221       5
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2728    3556    4060    6159 
   Size:       2728     828     504    2099

  Fragments arranged by size: 

                2728    2099     828     504
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2572    2604    6159 
   Size:       2572      32    3555

  Fragments arranged by size: 

                3555    2572      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     661     812    2238    3501    3783    3955    5059    5590 
   Size:        661     151    1426    1263     282     172    1104     531

Cuts at:   5590    6159 
   Size:        569

  Fragments arranged by size: 

                1426    1263    1104     661     569     531     282     172
                 151
 
ApaI G_GGCC'C

Cuts at:      0    1326    2966    3227    3297    4957    5880    6046    6159 
   Size:       1326    1640     261      70    1660     923     166     113

  Fragments arranged by size: 

                1660    1640    1326     923     261     166     113      70
            
 
ApaLI G'TGCA_C

Cuts at:      0    1452    4086    6159 
   Size:       1452    2634    2073

  Fragments arranged by size: 

                2634    2073    1452
 
ApoI r'AATT_y

Cuts at:      0    1807    1945    6159 
   Size:       1807     138    4214

  Fragments arranged by size: 

                4214    1807     138
 
AvaI C'yCGr_G

Cuts at:      0     747    1261    1860    1920    3691    5435    6082    6159 
   Size:        747     514     599      60    1771    1744     647      77

  Fragments arranged by size: 

                1771    1744     747     647     599     514      77      60
            
 
BanI G'GyrC_C

Cuts at:      0      64     132     176     868    1297    1390    2320    3024 
   Size:         64      68      44     692     429      93     930     704

Cuts at:   3024    3889    4013    5539    6159 
   Size:        865     124    1526     620

  Fragments arranged by size: 

                1526     930     865     704     692     620     429     124
                  93      68      64      44
 
BanII G_rGCy'C

Cuts at:      0     154     343    1021    1097    1326    1340    1957    2660 
   Size:        154     189     678      76     229      14     617     703

Cuts at:   2660    2930    2966    3227    3297    3341    4387    4436    4957 
   Size:        270      36     261      70      44    1046      49     521

Cuts at:   4957    5311    5756    5880    6046    6067    6159 
   Size:        354     445     124     166      21      92

  Fragments arranged by size: 

                1046     703     678     617     521     445     354     270
                 261     229     189     166     154     124      92      76
                  70      49      44      36      21      14
 
BbeI G_GCGC'C

Cuts at:      0    1301    6159 
   Size:       1301    4858
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     420     979    3989    6159 
   Size:        420     559    3010    2170

  Fragments arranged by size: 

                3010    2170     559     420
 
BbvCI CC'TCA_GC

Cuts at:      0    4064    4182    6159 
   Size:       4064     118    1977

  Fragments arranged by size: 

                4064    1977     118
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1566    1600    3226    3260    6159 
   Size:       1566      34    1626      34    2899

  Fragments arranged by size: 

                2899    1626    1566      34      34
 
BclI T'GATC_A

Cuts at:      0    1907    2369    6159 
   Size:       1907     462    3790

  Fragments arranged by size: 

                3790    1907     462
 
BfrBI ATG'CAT

Cuts at:      0    2006    6159 
   Size:       2006    4153
 
BglI GCCn_nnn'nGGC

Cuts at:      0    6010    6159 
   Size:       6010     149
 
BglII A'GATC_T

Cuts at:      0    1964    6159 
   Size:       1964    4195
 
BlpI GC'TnA_GC

Cuts at:      0    1096    5978    6159 
   Size:       1096    4882     181

  Fragments arranged by size: 

                4882    1096     181
 
Bme1580I G_kGCm'C

Cuts at:      0     137     642    1011    1326    1456    1787    2066    2216 
   Size:        137     505     369     315     130     331     279     150

Cuts at:   2216    2966    3227    3269    3297    3892    4016    4090    4957 
   Size:        750     261      42      28     595     124      74     867

Cuts at:   4957    5663    5880    6046    6159 
   Size:        706     217     166     113

  Fragments arranged by size: 

                 867     750     706     595     505     369     331     315
                 279     261     217     166     150     137     130     124
                 113      74      42      28
 
BmgBI CAC'GTC

Cuts at:      0     207     225    1689    2268    3315    5749    6159 
   Size:        207      18    1464     579    1047    2434     410

  Fragments arranged by size: 

                2434    1464    1047     579     410     207      18
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3174    4686    4819    5032    6159 
   Size:       3174    1512     133     213    1127

  Fragments arranged by size: 

                3174    1512    1127     213     133
 
BmtI G_CTAG'C

Cuts at:      0    3614    6159 
   Size:       3614    2545
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1098    1290    2562    5427    6159 
   Size:       1098     192    1272    2865     732

  Fragments arranged by size: 

                2865    1272    1098     732     192
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1908    2698    3277    4287    4564    5479    6051    6159 
   Size:       1908     790     579    1010     277     915     572     108

  Fragments arranged by size: 

                1908    1010     915     790     579     572     277     108
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    4064    4182    6159 
   Size:       4064     118    1977

  Fragments arranged by size: 

                4064    1977     118
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     763    1228    1660    1966    2545    3151    4318    4730 
   Size:        763     465     432     306     579     606    1167     412

Cuts at:   4730    5217    6159 
   Size:        487     942

  Fragments arranged by size: 

                1167     942     763     606     579     487     465     432
                 412     306
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     613    6116    6159 
   Size:        613    5503      43

  Fragments arranged by size: 

                5503     613      43
 
BsaAI yAC'GTr

Cuts at:      0    1770    4085    4346    4634    6159 
   Size:       1770    2315     261     288    1525

  Fragments arranged by size: 

                2315    1770    1525     288     261
 
BsaHI Gr'CG_yC

Cuts at:      0     572    1298    3362    5102    6159 
   Size:        572     726    2064    1740    1057

  Fragments arranged by size: 

                2064    1740    1057     726     572
 
BsaWI w'CCGG_w

Cuts at:      0     335    1448    3749    5225    5789    6159 
   Size:        335    1113    2301    1476     564     370

  Fragments arranged by size: 

                2301    1476    1113     564     370     335
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     244     274     481     511     562     592    1705    1735 
   Size:        244      30     207      30      51      30    1113      30

Cuts at:   1735    4459    4489    6012    6042    6159 
   Size:       2724      30    1523      30     117

  Fragments arranged by size: 

                2724    1523    1113     244     207     117      51      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2959    4291    5440    5476    6159 
   Size:       2959    1332    1149      36     683

  Fragments arranged by size: 

                2959    1332    1149     683      36
 
BseYI C'CCAG_C

Cuts at:      0     157    1516    1742    2195    2462    2723    3268    3517 
   Size:        157    1359     226     453     267     261     545     249

Cuts at:   3517    3712    3864    5312    5937    5955    5995    6016    6159 
   Size:        195     152    1448     625      18      40      21     143

  Fragments arranged by size: 

                1448    1359     625     545     453     267     261     249
                 226     195     157     152     143      40      21      18
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     475     723    1711    1743    2412    4073    6159 
   Size:        291     184     248     988      32     669    1661    2086

  Fragments arranged by size: 

                2086    1661     988     669     291     248     184      32
            
 
BsiEI CG_ry'CG

Cuts at:      0     146    3816    6159 
   Size:        146    3670    2343

  Fragments arranged by size: 

                3670    2343     146
 
BsiHKAI G_wGCw'C

Cuts at:      0    1456    1957    2126    2930    3545    4090    5137    5578 
   Size:       1456     501     169     804     615     545    1047     441

Cuts at:   5578    6094    6159 
   Size:        516      65

  Fragments arranged by size: 

                1456    1047     804     615     545     516     501     441
                 169      65
 
BsiWI C'GTAC_G

Cuts at:      0    3528    4833    6159 
   Size:       3528    1305    1326

  Fragments arranged by size: 

                3528    1326    1305
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1099    1236    1980    2550    3718    5109    6159 
   Size:       1099     137     744     570    1168    1391    1050

  Fragments arranged by size: 

                1391    1168    1099    1050     744     570     137
 
Bsp1286I G_dGCh'C

Cuts at:      0     137     154     343     642    1011    1021    1097    1326 
   Size:        137      17     189     299     369      10      76     229

Cuts at:   1326    1340    1456    1787    1957    2066    2126    2216    2660 
   Size:         14     116     331     170     109      60      90     444

Cuts at:   2660    2930    2966    3227    3269    3297    3341    3545    3892 
   Size:        270      36     261      42      28      44     204     347

Cuts at:   3892    4016    4090    4387    4436    4957    5137    5311    5578 
   Size:        124      74     297      49     521     180     174     267

Cuts at:   5578    5663    5756    5880    6046    6067    6094    6159 
   Size:         85      93     124     166      21      27      65

  Fragments arranged by size: 

                 521     444     369     347     331     299     297     270
                 267     261     229     204     189     180     174     170
                 166     137     124     124     116     109      93      90
                  85      76      74      65      60      49      44      42
                  36      28      27      21      17      14      10
 
BspEI T'CCGG_A

Cuts at:      0     335    5789    6159 
   Size:        335    5454     370

  Fragments arranged by size: 

                5454     370     335
 
BspHI T'CATG_A

Cuts at:      0    1949    6159 
   Size:       1949    4210
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      90    2720    4505    5280    5708    6159 
   Size:         90    2630    1785     775     428     451

  Fragments arranged by size: 

                2630    1785     775     451     428      90
 
BsrBI CCG'CTC

Cuts at:      0    2186    2445    2953    3450    6159 
   Size:       2186     259     508     497    2709

  Fragments arranged by size: 

                2709    2186     508     497     259
 
BsrDI GCAATG_nn'

Cuts at:      0     639    1199    4686    6159 
   Size:        639     560    3487    1473

  Fragments arranged by size: 

                3487    1473     639     560
 
BsrFI r'CCGG_y

Cuts at:      0     325     715     788     976    1448    3705    3811    4016 
   Size:        325     390      73     188     472    2257     106     205

Cuts at:   4016    4550    5225    5941    6159 
   Size:        534     675     716     218

  Fragments arranged by size: 

                2257     716     675     534     472     390     325     218
                 205     188     106      73
 
BsrGI T'GTAC_A

Cuts at:      0     545    2870    6159 
   Size:        545    2325    3289

  Fragments arranged by size: 

                3289    2325     545
 
BssSI C'ACGA_G

Cuts at:      0    6032    6159 
   Size:       6032     127
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3783    6159 
   Size:       3783    2376
 
BstBI TT'CG_AA

Cuts at:      0    1805    5733    6159 
   Size:       1805    3928     426

  Fragments arranged by size: 

                3928    1805     426
 
BstEII G'GTnAC_C

Cuts at:      0     102     885    1314    3012    3837    6159 
   Size:        102     783     429    1698     825    2322

  Fragments arranged by size: 

                2322    1698     825     783     429     102
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     120     684    2847    3255    4033    4542    6159 
   Size:        120     564    2163     408     778     509    1617

  Fragments arranged by size: 

                2163    1617     778     564     509     408     120
 
BstYI r'GATC_y

Cuts at:      0     332    1427    1964    2234    2357    2681    3945    6159 
   Size:        332    1095     537     270     123     324    1264    2214

  Fragments arranged by size: 

                2214    1264    1095     537     332     324     270     123
            
 
BtgI C'CryG_G

Cuts at:      0     119     146     311     534     725    1089    1310    2574 
   Size:        119      27     165     223     191     364     221    1264

Cuts at:   2574    2969    3131    3768    4004    4422    4957    5303    5762 
   Size:        395     162     637     236     418     535     346     459

Cuts at:   5762    6159 
   Size:        397

  Fragments arranged by size: 

                1264     637     535     459     418     397     395     364
                 346     236     223     221     191     165     162     119
                  27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1992    2379    2499    6159 
   Size:       1992     387     120    3660

  Fragments arranged by size: 

                3660    1992     387     120
 
BtsI GCAGTG_nn'

Cuts at:      0    1482    1731    2916    3543    3621    4039    4061    4819 
   Size:       1482     249    1185     627      78     418      22     758

Cuts at:   4819    6159 
   Size:       1340

  Fragments arranged by size: 

                1482    1340    1185     758     627     418     249      78
                  22
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    4086    4121    6159 
   Size:       4086      35    2038

  Fragments arranged by size: 

                4086    2038      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     726    2211    2440    5498    5776    6159 
   Size:        726    1485     229    3058     278     383

  Fragments arranged by size: 

                3058    1485     726     383     278     229
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2542    3145    3319    6159 
   Size:       2542     603     174    2840

  Fragments arranged by size: 

                2840    2542     603     174
 
EaeI y'GGCC_r

Cuts at:      0     143     182     422     717     786    1289    1827    2223 
   Size:        143      39     240     295      69     503     538     396

Cuts at:   2223    2363    2904    3317    3699    3794    3813    3989    4091 
   Size:        140     541     413     382      95      19     176     102

Cuts at:   4091    4802    5167    5921    6159 
   Size:        711     365     754     238

  Fragments arranged by size: 

                 754     711     541     538     503     413     396     382
                 365     295     240     238     176     143     140     102
                  95      69      39      19
 
EagI C'GGCC_G

Cuts at:      0     143    3813    6159 
   Size:        143    3670    2346

  Fragments arranged by size: 

                3670    2346     143
 
EarI CTCTTCn'nnn_

Cuts at:      0     453    1126    2270    3234    3748    4465    4831    4837 
   Size:        453     673    1144     964     514     717     366       6

Cuts at:   4837    5418    6159 
   Size:        581     741

  Fragments arranged by size: 

                1144     964     741     717     673     581     514     453
                 366       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     258    2251    6159 
   Size:        258    1993    3908

  Fragments arranged by size: 

                3908    1993     258
 
EcoICRI GAG'CTC

Cuts at:      0    1955    2928    6159 
   Size:       1955     973    3231

  Fragments arranged by size: 

                3231    1955     973
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      74     434     991    1224    1908    2575    2577    2698 
   Size:         74     360     557     233     684     667       2     121

Cuts at:   2698    3277    4287    4425    4564    4882    5479    6051    6159 
   Size:        579    1010     138     139     318     597     572     108

  Fragments arranged by size: 

                1010     684     667     597     579     572     557     360
                 318     233     139     138     121     108      74       2
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2275    2299    4937    5362    6159 
   Size:       2275      24    2638     425     797

  Fragments arranged by size: 

                2638    2275     797     425      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1145    1323    1420    1670    2963    3223    3224    3294 
   Size:       1145     178      97     250    1293     260       1      70

Cuts at:   3294    3574    3861    4562    4578    4953    5140    5876    5877 
   Size:        280     287     701      16     375     187     736       1

Cuts at:   5877    6042    6131    6159 
   Size:        165      89      28

  Fragments arranged by size: 

                1293    1145     736     701     375     287     280     260
                 250     187     178     165      97      89      70      28
                  16       1       1
 
EcoRI G'AATT_C

Cuts at:      0    1807    1945    6159 
   Size:       1807     138    4214

  Fragments arranged by size: 

                4214    1807     138
 
FseI GG_CCGG'CC

Cuts at:      0     719     792    6159 
   Size:        719      73    5367

  Fragments arranged by size: 

                5367     719      73
 
FspI TGC'GCA

Cuts at:      0    4648    6159 
   Size:       4648    1511
 
HaeII r_GCGC'y

Cuts at:      0    1301    2046    4314    6002    6159 
   Size:       1301     745    2268    1688     157

  Fragments arranged by size: 

                2268    1688    1301     745     157
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     388     420     481     513     562     594    1892    1924 
   Size:        388      32      61      32      49      32    1298      32

Cuts at:   1924    5132    5164    5503    5535    6159 
   Size:       3208      32     339      32     624

  Fragments arranged by size: 

                3208    1298     624     388     339      61      49      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1257    4128    5079    6159 
   Size:       1257    2871     951    1080

  Fragments arranged by size: 

                2871    1257    1080     951
 
HindIII A'AGCT_T

Cuts at:      0    4920    6159 
   Size:       4920    1239
 
KasI G'GCGC_C

Cuts at:      0    1297    6159 
   Size:       1297    4862
 
KpnI G_GTAC'C

Cuts at:      0     180    6159 
   Size:        180    5979
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1364    2664    5315    6159 
   Size:       1364    1300    2651     844

  Fragments arranged by size: 

                2651    1364    1300     844
 
MscI TGG'CCA

Cuts at:      0     184    3701    4804    5923    6159 
   Size:        184    3517    1103    1119     236

  Fragments arranged by size: 

                3517    1119    1103     236     184
 
MslI CAynn'nnrTG

Cuts at:      0     293     925    1281    2881    3010    3997    4031    4933 
   Size:        293     632     356    1600     129     987      34     902

Cuts at:   4933    5047    5152    6055    6154    6159 
   Size:        114     105     903      99       5

  Fragments arranged by size: 

                1600     987     903     902     632     356     293     129
                 114     105      99      34       5
 
MspA1I CmG'CkG

Cuts at:      0     148     313     773     812     866    1040    1833    2462 
   Size:        148     165     460      39      54     174     793     629

Cuts at:   2462    2569    2597    3770    4989    5469    5884    6159 
   Size:        107      28    1173    1219     480     415     275

  Fragments arranged by size: 

                1219    1173     793     629     480     460     415     275
                 174     165     148     107      54      39      28
 
NaeI GCC'GGC

Cuts at:      0     327     717     790    3707    3813    5943    6159 
   Size:        327     390      73    2917     106    2130     216

  Fragments arranged by size: 

                2917    2130     390     327     216     106      73
 
NarI GG'CG_CC

Cuts at:      0    1298    6159 
   Size:       1298    4861
 
NcoI C'CATG_G

Cuts at:      0    2969    4004    6159 
   Size:       2969    1035    2155

  Fragments arranged by size: 

                2969    2155    1035
 
NgoMIV G'CCGG_C

Cuts at:      0     325     715     788    3705    3811    5941    6159 
   Size:        325     390      73    2917     106    2130     218

  Fragments arranged by size: 

                2917    2130     390     325     218     106      73
 
NheI G'CTAG_C

Cuts at:      0    3610    6159 
   Size:       3610    2549
 
NsiI A_TGCA'T

Cuts at:      0    2008    6159 
   Size:       2008    4151
 
NspI r_CATG'y

Cuts at:      0    1872    2006    2176    2886    4486    5157    5452    6159 
   Size:       1872     134     170     710    1600     671     295     707

  Fragments arranged by size: 

                1872    1600     710     707     671     295     170     134
            
 
PasI CC'CwG_GG

Cuts at:      0    4950    4979    5872    6159 
   Size:       4950      29     893     287

  Fragments arranged by size: 

                4950     893     287      29
 
PciI A'CATG_T

Cuts at:      0    5153    6159 
   Size:       5153    1006
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     726     773    1040    1610    3501    6159 
   Size:        726      47     267     570    1891    2658

  Fragments arranged by size: 

                2658    1891     726     570     267      47
 
PfoI T'CCnGG_A

Cuts at:      0      26     683    2684    5188    6159 
   Size:         26     657    2001    2504     971

  Fragments arranged by size: 

                2504    2001     971     657      26
 
PmlI CAC'GTG

Cuts at:      0    4085    4346    4634    6159 
   Size:       4085     261     288    1525

  Fragments arranged by size: 

                4085    1525     288     261
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    5215    5247    6159 
   Size:       5215      32     912

  Fragments arranged by size: 

                5215     912      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1670    3574    4562    5140    6159 
   Size:       1670    1904     988     578    1019

  Fragments arranged by size: 

                1904    1670    1019     988     578
 
PspOMI G'GGCC_C

Cuts at:      0    1322    2962    3223    3293    4953    5876    6042    6159 
   Size:       1322    1640     261      70    1660     923     166     117

  Fragments arranged by size: 

                1660    1640    1322     923     261     166     117      70
            
 
PstI C_TGCA'G

Cuts at:      0     248    1579    1964    3044    4502    5474    6159 
   Size:        248    1331     385    1080    1458     972     685

  Fragments arranged by size: 

                1458    1331    1080     972     685     385     248
 
PvuII CAG'CTG

Cuts at:      0    1833    2597    4989    5469    6159 
   Size:       1833     764    2392     480     690

  Fragments arranged by size: 

                2392    1833     764     690     480
 
RsrII CG'GwC_CG

Cuts at:      0     854    5637    6159 
   Size:        854    4783     522

  Fragments arranged by size: 

                4783     854     522
 
SacI G_AGCT'C

Cuts at:      0    1957    2930    6159 
   Size:       1957     973    3229

  Fragments arranged by size: 

                3229    1957     973
 
SacII CC_GC'GG

Cuts at:      0     149     314    3771    6159 
   Size:        149     165    3457    2388

  Fragments arranged by size: 

                3457    2388     165     149
 
SanDI GG'GwC_CC

Cuts at:      0    1670    6159 
   Size:       1670    4489
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1126    3748    4831    6159 
   Size:       1126    2622    1083    1328

  Fragments arranged by size: 

                2622    1328    1126    1083
 
SbfI CC_TGCA'GG

Cuts at:      0    4502    6159 
   Size:       4502    1657
 
ScaI AGT'ACT

Cuts at:      0    4258    6159 
   Size:       4258    1901
 
SfcI C'TryA_G

Cuts at:      0     244    1575    1960    3040    3075    3486    4252    4498 
   Size:        244    1331     385    1080      35     411     766     246

Cuts at:   4498    5346    5470    6159 
   Size:        848     124     689

  Fragments arranged by size: 

                1331    1080     848     766     689     411     385     246
                 244     124      35
 
SfoI GGC'GCC

Cuts at:      0    1299    6159 
   Size:       1299    4860
 
SmaI CCC'GGG

Cuts at:      0    1263    3693    5437    6159 
   Size:       1263    2430    1744     722

  Fragments arranged by size: 

                2430    1744    1263     722
 
SmlI C'TyrA_G

Cuts at:      0     778    1207    1675    1981    2560    3166    4333    4745 
   Size:        778     429     468     306     579     606    1167     412

Cuts at:   4745    5232    6082    6159 
   Size:        487     850      77

  Fragments arranged by size: 

                1167     850     778     606     579     487     468     429
                 412     306      77
 
SphI G_CATG'C

Cuts at:      0    2176    4486    5452    6159 
   Size:       2176    2310     966     707

  Fragments arranged by size: 

                2310    2176     966     707
 
SrfI GCCC'GGGC

Cuts at:      0    5437    6159 
   Size:       5437     722
 
StuI AGG'CCT

Cuts at:      0    5263    5725    5782    6159 
   Size:       5263     462      57     377

  Fragments arranged by size: 

                5263     462     377      57
 
StyI C'CwwG_G

Cuts at:      0    1292    2156    2177    2522    2969    3557    3904    3971 
   Size:       1292     864      21     345     447     588     347      67

Cuts at:   3971    4004    4556    5855    6159 
   Size:         33     552    1299     304

  Fragments arranged by size: 

                1299    1292     864     588     552     447     347     345
                 304      67      33      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     871    1530    2169    3042    3526    3780    4480    4746 
   Size:        871     659     639     873     484     254     700     266

Cuts at:   4746    5132    5634    5830    5885    6000    6159 
   Size:        386     502     196      55     115     159

  Fragments arranged by size: 

                 873     871     700     659     639     502     484     386
                 266     254     196     159     115      55
 
TatI w'GTAC_w

Cuts at:      0     545    1373    2870    2885    3107    3668    4256    5095 
   Size:        545     828    1497      15     222     561     588     839

Cuts at:   5095    6159 
   Size:       1064

  Fragments arranged by size: 

                1497    1064     839     828     588     561     545     222
                  15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1505    1537    1705    1737    2990    3022    4200    4232 
   Size:       1505      32     168      32    1253      32    1178      32

Cuts at:   4232    4459    4491    6012    6044    6159 
   Size:        227      32    1521      32     115

  Fragments arranged by size: 

                1521    1505    1253    1178     227     168     115      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     493    2479    2497    2539    6159 
   Size:        493    1986      18      42    3620

  Fragments arranged by size: 

                3620    1986     493      42      18
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    4377    4812    5793    5853    6159 
   Size:       4377     435     981      60     306

  Fragments arranged by size: 

                4377     981     435     306      60
 
XhoI C'TCGA_G

Cuts at:      0    6082    6159 
   Size:       6082      77
 
XmaI C'CCGG_G

Cuts at:      0    1261    3691    5435    6159 
   Size:       1261    2430    1744     724

  Fragments arranged by size: 

                2430    1744    1261     724
 
XmnI GAAnn'nnTTC

Cuts at:      0    4774    6159 
   Size:       4774    1385
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FseI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NsiI     NspI     PasI     PciI
    PflMI     PfoI     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII
    RsrII     SacI    SacII    SanDI     SapI     SbfI     ScaI     SfcI
     SfoI     SmaI     SmlI     SphI     SrfI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AhdI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BciVI    BsaBI     BsmI   BssHII  BstZ17I   Bsu36I
     ClaI     DraI    EcoRV     FalI    FspAI     HpaI     MfeI     MluI
     NdeI     NotI     NruI     PacI     PmeI    PshAI     PsiI    PspXI
     PsrI     PvuI     SalI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SspI     SwaI     XbaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com