Restriction map for pF1KA1116
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbrPI       BbvCI       
BcgI        BfrI        BlnI        BsaAI       BseAI       
BsiWI       Bsp1407I    BspEI       BspHI       BspMI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstPI       BstUI       BstXI       
BstZ17I     CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
FseI        FspI        HindIII     HpaI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
SfiI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        Tth111I     
XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AcyI         1935  GGCGCC               G[GA]^CG[CT]C
AflIII        123  ACATGT               A^C[GA][CT]GT
AspHI        1580  GAGCTC               G[AT]GC[AT]^C
BalI          440  TGGCCA               TGG^CCA
BbeI         1935  GGCGCC               GGCGC^C
BclI         1770  TGATCA               T^GATCA
BglI         3273  GCCCTGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         3667  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3667  GCTCAGC              GC^T[ACGT]AGC
BsaBI        1687  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1935  GGCGCC               G[GA]^CG[CT]C
BsaI         3245  GAGACC               GGTCTC(1/5)
BsgI         2146  GTGCAG               GTGCAG(16/14)
BsiEI        1330  CGGTCG               CG[GA][CT]^CG
BsiHKAI      1580  GAGCTC               G[AT]GC[AT]^C
BsmBI        1828  GAGACG               CGTCTC(1/5)
BsmI         1023  GCATTC               GAATGC(1/-1)
BspDI        3383  ATCGAT               AT^CGAT
BspLU11I      123  ACATGT               A^CATGT
BstBI          63  TTCGAA               TT^CGAA
CelII        3667  GCTCAGC              GC^T[ACGT]AGC
CfoI         1936  GCGC                 GCG^C
ClaI         3383  ATCGAT               AT^CGAT
DraI         1647  TTTAAA               TTT^AAA
EcoNI        3464  CCTGGGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      1571  ATGCAT               ATGCA^T
FauI         1298  CCCGC                CCCGC(4/6)
FbaI         1770  TGATCA               T^GATCA
HaeII        1935  GGCGCC               [GA]GCGC^[CT]
HgaI         1030  GCGTC                GACGC(5/10)
HhaI         1936  GCGC                 GCG^C
Hin1I        1935  GGCGCC               G[GA]^CG[CT]C
HinP1I       1936  GCGC                 G^CGC
HincII       2930  GTCAAC               GT[CT]^[GA]AC
HindII       2930  GTCAAC               GT[CT]^[GA]AC
KasI         1935  GGCGCC               G^GCGCC
MaeII         869  ACGT                 A^CGT
MamI         1687  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         440  TGGCCA               TGG^CCA
MscI          440  TGGCCA               TGG^CCA
NarI         1935  GGCGCC               GG^CGCC
NcoI         1714  CCATGG               C^CATGG
NsiI         1571  ATGCAT               ATGCA^T
NspV           63  TTCGAA               TT^CGAA
PpuMI        3141  AGGTCCT              [GA]G^G[AT]CC[CT]
SacI         1580  GAGCTC               GAGCT^C
SexAI        2067  ACCTGGT              A^CC[AT]GGT
SfoI         1935  GGCGCC               GGC^GCC
SfuI           63  TTCGAA               TT^CGAA
SmaI         1532  CCCGGG               CCC^GGG
XbaI         1264  TCTAGA               T^CTAGA
XmaCI        1532  CCCGGG               C^CCGGG
XmaI         1532  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         2971  GTAGAC               GT^[AC][GT]AC
             3083  GTAGAC              
AgeI         3047  ACCGGT               A^CCGGT
             3321  ACCGGT              
AocI          988  CCTCAGG              CC^T[ACGT]AGG
             3634  CCTCAGG             
AseI          615  ATTAAT               AT^TAAT
             1516  ATTAAT              
AsnI          615  ATTAAT               AT^TAAT
             1516  ATTAAT              
Asp700I        12  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1202  GAACACATTC          
AvaI         1532  CCCGGG               C^[CT]CG[GA]G
             3578  CTCGGG              
BanI         1935  GGCGCC               G^G[CT][GA]CC
             2799  GGCACC              
BanII        1580  GAGCTC               G[GA]GC[CT]^C
             1848  GAGCCC              
BciVI        2999  GGATAC               GTATCC(6/5)
             2735  GTATCC              
BglII         578  AGATCT               A^GATCT
             1402  AGATCT              
BmyI         1580  GAGCTC               G[AGT]GC[ACT]^C
             1848  GAGCCC              
BsoBI        1532  CCCGGG               C^[CT]CG[GA]G
             3578  CTCGGG              
Bsp1286I     1580  GAGCTC               G[AGT]GC[ACT]^C
             1848  GAGCCC              
BsrBI        1299  CCGCTC               CCGCTC(-3/-3)
             2246  CCGCTC              
Bsu36I        988  CCTCAGG              CC^T[ACGT]AGG
             3634  CCTCAGG             
DraII         668  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             3141  AGGTCCT             
Eco81I        988  CCTCAGG              CC^T[ACGT]AGG
             3634  CCTCAGG             
EcoO109I      668  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             3141  AGGTCCT             
MslI          453  CACTGCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1515  CATTAATATG          
NspI          123  ACATGT               [GA]CATG^[CT]
             3663  ACATGC              
PinAI        3047  ACCGGT               A^CCGGT
             3321  ACCGGT              
PshAI        1872  GACAGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2773  GACATTCGTC          
PshBI         615  ATTAAT               AT^TAAT
             1516  ATTAAT              
SapI         1582  GCTCTTC              GCTCTTC(1/4)
             2881  GCTCTTC             
ScaI          336  AGTACT               AGT^ACT
             1426  AGTACT              
SspI         2539  AATATT               AAT^ATT
             2559  AATATT              
XcmI         2328  CCAGATTTCTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3265  CCAGAGAAGCCCTGG     
XmnI           12  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1202  GAACACATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaII       BbsI        BbvI        
BcnI        BfaI        BpmI        BpuAI       BsaJI       
BsaWI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr10I      Cfr13I      
DdeI        DpnII       DraIII      DsaI        EaeI        
EarI        EcoRI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com