Restriction map for pF1KA1116
 (Linear) (Six-base) MAPSORT of: KIAA1116.seq  Check: 5652  from: 1  to: 3813



KIAA1116.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:20  ..

AccI GT'mk_AC

Cuts at:      0    2972    3084    3813 
   Size:       2972     112     729

  Fragments arranged by size: 

                2972     729     112
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1094    1570    2318    3813 
   Size:       1094     476     748    1495

  Fragments arranged by size: 

                1495    1094     748     476
 
AflIII A'CryG_T

Cuts at:      0     123    3813 
   Size:        123    3690
 
AgeI A'CCGG_T

Cuts at:      0    3047    3321    3813 
   Size:       3047     274     492

  Fragments arranged by size: 

                3047     492     274
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3416    3448    3813 
   Size:       3416      32     365

  Fragments arranged by size: 

                3416     365      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     880    1819    2296    3763    3802    3813 
   Size:        880     939     477    1467      39      11

  Fragments arranged by size: 

                1467     939     880     477      39      11
 
ApoI r'AATT_y

Cuts at:      0     291     845    2432    2474    2834    3545    3813 
   Size:        291     554    1587      42     360     711     268

  Fragments arranged by size: 

                1587     711     554     360     291     268      42
 
AseI AT'TA_AT

Cuts at:      0     616    1517    3813 
   Size:        616     901    2296

  Fragments arranged by size: 

                2296     901     616
 
AvaI C'yCGr_G

Cuts at:      0    1532    3578    3813 
   Size:       1532    2046     235

  Fragments arranged by size: 

                2046    1532     235
 
BanI G'GyrC_C

Cuts at:      0    1935    2799    3813 
   Size:       1935     864    1014

  Fragments arranged by size: 

                1935    1014     864
 
BanII G_rGCy'C

Cuts at:      0    1584    1852    3813 
   Size:       1584     268    1961

  Fragments arranged by size: 

                1961    1584     268
 
BbeI G_GCGC'C

Cuts at:      0    1939    3813 
   Size:       1939    1874
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      19     818    2967    3189    3465    3813 
   Size:         19     799    2149     222     276     348

  Fragments arranged by size: 

                2149     799     348     276     222      19
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2746    2993    3813 
   Size:       2746     247     820

  Fragments arranged by size: 

                2746     820     247
 
BclI T'GATC_A

Cuts at:      0    1770    3813 
   Size:       1770    2043
 
BfrBI ATG'CAT

Cuts at:      0    1573    3813 
   Size:       1573    2240
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3279    3813 
   Size:       3279     534
 
BglII A'GATC_T

Cuts at:      0     578    1402    3813 
   Size:        578     824    2411

  Fragments arranged by size: 

                2411     824     578
 
BlpI GC'TnA_GC

Cuts at:      0    3668    3813 
   Size:       3668     145
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2573    3813 
   Size:       2573    1240
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     485    1871    3683    3813 
   Size:        485    1386    1812     130

  Fragments arranged by size: 

                1812    1386     485     130
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     404     455    1841    2069    2897    3813 
   Size:        404      51    1386     228     828     916

  Fragments arranged by size: 

                1386     916     828     404     228      51
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578    1176    1287    3813 
   Size:        578     598     111    2526

  Fragments arranged by size: 

                2526     598     578     111
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3239    3813 
   Size:       3239     574
 
BsaBI GATnn'nnATC

Cuts at:      0    1691    3813 
   Size:       1691    2122
 
BsaHI Gr'CG_yC

Cuts at:      0    1936    3813 
   Size:       1936    1877
 
BsaWI w'CCGG_w

Cuts at:      0    2169    3047    3321    3813 
   Size:       2169     878     274     492

  Fragments arranged by size: 

                2169     878     492     274
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     735     765    3813 
   Size:        735      30    3048

  Fragments arranged by size: 

                3048     735      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     977    1165    1895    1988    3302    3560    3813 
   Size:        977     188     730      93    1314     258     253

  Fragments arranged by size: 

                1314     977     730     258     253     188      93
 
BseYI C'CCAG_C

Cuts at:      0     650    3813 
   Size:        650    3163
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2167    3813 
   Size:       2167    1646
 
BsiEI CG_ry'CG

Cuts at:      0    1333    3813 
   Size:       1333    2480
 
BsiHKAI G_wGCw'C

Cuts at:      0    1584    3813 
   Size:       1584    2229
 
BsmI GAATG_Cn'

Cuts at:      0    1023    3813 
   Size:       1023    2790
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1822    3813 
   Size:       1822    1991
 
Bsp1286I G_dGCh'C

Cuts at:      0    1584    1852    3813 
   Size:       1584     268    1961

  Fragments arranged by size: 

                1961    1584     268
 
BsrBI CCG'CTC

Cuts at:      0    1301    2248    3813 
   Size:       1301     947    1565

  Fragments arranged by size: 

                1565    1301     947
 
BsrFI r'CCGG_y

Cuts at:      0    1989    3047    3321    3813 
   Size:       1989    1058     274     492

  Fragments arranged by size: 

                1989    1058     492     274
 
BstBI TT'CG_AA

Cuts at:      0      64    3813 
   Size:         64    3749
 
BstYI r'GATC_y

Cuts at:      0     578    1402    1692    3129    3813 
   Size:        578     824     290    1437     684

  Fragments arranged by size: 

                1437     824     684     578     290
 
Bsu36I CC'TnA_GG

Cuts at:      0     989    3635    3813 
   Size:        989    2646     178

  Fragments arranged by size: 

                2646     989     178
 
BtgI C'CryG_G

Cuts at:      0     529    1714    2898    3813 
   Size:        529    1185    1184     915

  Fragments arranged by size: 

                1185    1184     915     529
 
BtsI GCAGTG_nn'

Cuts at:      0     452    3813 
   Size:        452    3361
 
ClaI AT'CG_AT

Cuts at:      0    3384    3813 
   Size:       3384     429
 
DraI TTT'AAA

Cuts at:      0    1649    3813 
   Size:       1649    2164
 
DraIII CAC_nnn'GTG

Cuts at:      0    1417    2581    2899    3813 
   Size:       1417    1164     318     914

  Fragments arranged by size: 

                1417    1164     914     318
 
EaeI y'GGCC_r

Cuts at:      0     440     500    2488    3813 
   Size:        440      60    1988    1325

  Fragments arranged by size: 

                1988    1325     440      60
 
EarI CTCTTCn'nnn_

Cuts at:      0    1142    1487    1589    2888    3237    3742    3813 
   Size:       1142     345     102    1299     349     505      71

  Fragments arranged by size: 

                1299    1142     505     349     345     102      71
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     404    3813 
   Size:        404    3409
 
EcoICRI GAG'CTC

Cuts at:      0    1582    3813 
   Size:       1582    2231
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     404     455    1094    1570    1841    2069    2318    2897 
   Size:        404      51     639     476     271     228     249     579

Cuts at:   2897    3813 
   Size:        916

  Fragments arranged by size: 

                 916     639     579     476     404     271     249     228
                  51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3468    3813 
   Size:       3468     345
 
EcoO109I rG'GnC_Cy

Cuts at:      0     669    3142    3813 
   Size:        669    2473     671

  Fragments arranged by size: 

                2473     671     669
 
EcoRI G'AATT_C

Cuts at:      0    2432    2834    3545    3813 
   Size:       2432     402     711     268

  Fragments arranged by size: 

                2432     711     402     268
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2834    3422    3813 
   Size:       2834     588     391

  Fragments arranged by size: 

                2834     588     391
 
HaeII r_GCGC'y

Cuts at:      0    1939    3813 
   Size:       1939    1874
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     402     434     938     970    3294    3326    3813 
   Size:        402      32     504      32    2324      32     487

  Fragments arranged by size: 

                2324     504     487     402      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2932    3813 
   Size:       2932     881
 
KasI G'GCGC_C

Cuts at:      0    1935    3813 
   Size:       1935    1878
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2169    2302    3813 
   Size:       2169     133    1511

  Fragments arranged by size: 

                2169    1511     133
 
MscI TGG'CCA

Cuts at:      0     442    3813 
   Size:        442    3371
 
MslI CAynn'nnrTG

Cuts at:      0     457    1519    3813 
   Size:        457    1062    2294

  Fragments arranged by size: 

                2294    1062     457
 
MspA1I CmG'CkG

Cuts at:      0     724     730     736    2257    2707    3813 
   Size:        724       6       6    1521     450    1106

  Fragments arranged by size: 

                1521    1106     724     450       6       6
 
NarI GG'CG_CC

Cuts at:      0    1936    3813 
   Size:       1936    1877
 
NcoI C'CATG_G

Cuts at:      0    1714    3813 
   Size:       1714    2099
 
NsiI A_TGCA'T

Cuts at:      0    1575    3813 
   Size:       1575    2238
 
NspI r_CATG'y

Cuts at:      0     127    3667    3813 
   Size:        127    3540     146

  Fragments arranged by size: 

                3540     146     127
 
PasI CC'CwG_GG

Cuts at:      0     307    3275    3464    3813 
   Size:        307    2968     189     349

  Fragments arranged by size: 

                2968     349     307     189
 
PciI A'CATG_T

Cuts at:      0     123    3813 
   Size:        123    3690
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2068    2707    2899    3163    3813 
   Size:       2068     639     192     264     650

  Fragments arranged by size: 

                2068     650     639     264     192
 
PfoI T'CCnGG_A

Cuts at:      0     471    2001    2679    2781    2913    2955    3144    3813 
   Size:        471    1530     678     102     132      42     189     669

  Fragments arranged by size: 

                1530     678     669     471     189     132     102      42
            
 
PpuMI rG'GwC_Cy

Cuts at:      0    3142    3813 
   Size:       3142     671
 
PshAI GACnn'nnGTC

Cuts at:      0    1876    2777    3813 
   Size:       1876     901    1036

  Fragments arranged by size: 

                1876    1036     901
 
PstI C_TGCA'G

Cuts at:      0     668     729     735    3511    3813 
   Size:        668      61       6    2776     302

  Fragments arranged by size: 

                2776     668     302      61       6
 
PvuII CAG'CTG

Cuts at:      0     724     730     736    2257    2707    3813 
   Size:        724       6       6    1521     450    1106

  Fragments arranged by size: 

                1521    1106     724     450       6       6
 
SacI G_AGCT'C

Cuts at:      0    1584    3813 
   Size:       1584    2229
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1589    2888    3813 
   Size:       1589    1299     925

  Fragments arranged by size: 

                1589    1299     925
 
ScaI AGT'ACT

Cuts at:      0     338    1428    3813 
   Size:        338    1090    2385

  Fragments arranged by size: 

                2385    1090     338
 
SexAI A'CCwGG_T

Cuts at:      0    2067    3813 
   Size:       2067    1746
 
SfcI C'TryA_G

Cuts at:      0     569     664     725     731    1949    3059    3125    3191 
   Size:        569      95      61       6    1218    1110      66      66

Cuts at:   3191    3507    3813 
   Size:        316     306

  Fragments arranged by size: 

                1218    1110     569     316     306      95      66      66
                  61       6
 
SfoI GGC'GCC

Cuts at:      0    1937    3813 
   Size:       1937    1876
 
SmaI CCC'GGG

Cuts at:      0    1534    3813 
   Size:       1534    2279
 
SmlI C'TyrA_G

Cuts at:      0     593    1191    1302    3813 
   Size:        593     598     111    2511

  Fragments arranged by size: 

                2511     598     593     111
 
SspI AAT'ATT

Cuts at:      0    2541    2561    3813 
   Size:       2541      20    1252

  Fragments arranged by size: 

                2541    1252      20
 
StyI C'CwwG_G

Cuts at:      0     513    1622    1714    3406    3813 
   Size:        513    1109      92    1692     407

  Fragments arranged by size: 

                1692    1109     513     407      92
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      72     270    2592    3359    3813 
   Size:         72     198    2322     767     454

  Fragments arranged by size: 

                2322     767     454     198      72
 
TatI w'GTAC_w

Cuts at:      0     336    1426    2652    3687    3813 
   Size:        336    1090    1226    1035     126

  Fragments arranged by size: 

                1226    1090    1035     336     126
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     467     499     735     767    3546    3578    3813 
   Size:        467      32     236      32    2779      32     235

  Fragments arranged by size: 

                2779     467     236     235      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1264    3813 
   Size:       1264    2549
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2335    3272    3813 
   Size:       2335     937     541

  Fragments arranged by size: 

                2335     937     541
 
XmaI C'CCGG_G

Cuts at:      0    1532    3813 
   Size:       1532    2281
 
XmnI GAAnn'nnTTC

Cuts at:      0      16    1206    3813 
   Size:         16    1190    2607

  Fragments arranged by size: 

                2607    1190      16
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AgeI     AloI    AlwNI     ApoI     AseI
     AvaI     BanI    BanII     BbeI     BbsI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BsrBI    BsrFI    BstBI    BstYI
   Bsu36I     BtgI     BtsI     ClaI     DraI   DraIII     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII
    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NsiI     NspI     PasI     PciI    PflMI     PfoI    PpuMI
    PshAI     PstI    PvuII     SacI     SapI     ScaI    SexAI     SfcI
     SfoI     SmaI     SmlI     SspI     StyI    TaqII     TatI     TstI
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AhdI     AleI
     AlfI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI
    BbvCI     BcgI Bme1580I    BmgBI     BmtI   Bpu10I    BsaAI    BsiWI
    BspEI    BspHI    BspMI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstXI  BstZ17I    BtgZI    CspCI     DrdI     EagI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PmeI
     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI  Tth111I     XhoI     ZraI
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