Restriction map for
pF1KA1062
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# NO CUT ENZYMES ( 56 enzymes) #
##################################
AclI AcsI AflII AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I AspEI BfrI BsiWI BsmI
BspDI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV HindIII HpaI MfeI MluI
MseI MunI NdeI NheI NruI
NspV PacI PmeI PshBI Psp1406I
PvuI RsrII SfuI SgrAI SnaBI
SpeI SspI SwaI Tru9I XbaI
XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AvrII 645 CCTAGG C^CTAGG
BclI 6842 TGATCA T^GATCA
BfaI 646 CTAG C^TAG
BlnI 645 CCTAGG C^CTAGG
BsaBI 84 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 6449 TGTACA T^GTACA
BspHI 1355 TCATGA T^CATGA
BspLU11I 2483 ACATGT A^CATGT
BsrGI 6449 TGTACA T^GTACA
EagI 1950 CGGCCG C^GGCCG
EclXI 1950 CGGCCG C^GGCCG
Eco52I 1950 CGGCCG C^GGCCG
EcoT22I 3899 ATGCAT ATGCA^T
FbaI 6842 TGATCA T^GATCA
FseI 1227 GGCCGGCC GGCCGG^CC
KspI 5967 CCGCGG CCGC^GG
MaeI 646 CTAG C^TAG
MamI 84 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 1949 GCGGCCGC GC^GGCCGC
NsiI 3899 ATGCAT ATGCA^T
PaeR7I 358 CTCGAG C^TCGAG
PshAI 6165 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1355 TCATGA T^CATGA
SacII 5967 CCGCGG CCGC^GG
SalI 1402 GTCGAC G^TCGAC
SfiI 2427 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 6449 TGTACA T^GTACA
TfiI 601 GATTC G^A[AT]TC
XhoI 358 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 28 enzymes) #
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Acc65I 3644 GGTACC G^GTACC
6605 GGTACC
AccIII 665 TCCGGA T^CCGGA
857 TCCGGA
AgeI 5075 ACCGGT A^CCGGT
6821 ACCGGT
Asp718I 3644 GGTACC G^GTACC
6605 GGTACC
AviII 4929 TGCGCA TGC^GCA
5168 TGCGCA
BbeI 174 GGCGCC GGCGC^C
936 GGCGCC
BbvCI 3909 CCTCAGC CCTCAGC(-5/-2)
1144 GCTGAGG
BcgI 1428 CGAGACTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5366 GCACGGATGTCG
BciVI 1671 GGATAC GTATCC(6/5)
6197 GTATCC
BglII 83 AGATCT A^GATCT
4379 AGATCT
BseAI 665 TCCGGA T^CCGGA
857 TCCGGA
BspEI 665 TCCGGA T^CCGGA
857 TCCGGA
BsrDI 3570 CATTGC GCAATG(2/0)
3902 CATTGC
BssHII 2001 GCGCGC G^CGCGC
3469 GCGCGC
DrdI 2704 GACGCCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7033 GACTACTCGGTC
FspI 4929 TGCGCA TGC^GCA
5168 TGCGCA
KasI 174 GGCGCC G^GCGCC
936 GGCGCC
KpnI 3644 GGTACC GGTAC^C
6605 GGTACC
MroI 665 TCCGGA T^CCGGA
857 TCCGGA
NarI 174 GGCGCC GG^CGCC
936 GGCGCC
PinAI 5075 ACCGGT A^CCGGT
6821 ACCGGT
SbfI 777 CCTGCAGG CCTGCA^GG
4821 CCTGCAGG
ScaI 2588 AGTACT AGT^ACT
5903 AGTACT
SexAI 3050 ACCAGGT A^CC[AT]GGT
5540 ACCTGGT
SfoI 174 GGCGCC GGC^GCC
936 GGCGCC
SphI 5213 GCATGC GCATG^C
6056 GCATGC
Sse8387I 777 CCTGCAGG CCTGCA^GG
4821 CCTGCAGG
Tsp509I 4507 AATT ^AATT
4759 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaI ApaLI AspHI AspI
AvaI AvaII BalI BamHI BanI
BanII BbrPI BbsI BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TseI Tsp45I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI