Restriction map for
pF1KA1062
(Circular) (Six-base) MAPSORT of: pF1KA1062.seq Check: 3253 from: 1 to: 10418
pF1KA1062 10418 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 689 829 1649 3071 4738 5038 5585 6557 7104
Size: 140 820 1422 1667 300 547 972 547
Cuts at: 7104 689
Size: 4003
Fragments arranged by size:
4003 1667 1422 972 820 547 547 300
140
AatII G_ACGT'C
Cuts at: 295 4283 4984 295
Size: 3988 701 5729
Fragments arranged by size:
5729 3988 701
AccI GT'mk_AC
Cuts at: 674 1511 2255 2480 3101 6175 6281 7456 674
Size: 837 744 225 621 3074 106 1175 3636
Fragments arranged by size:
3636 3074 1175 837 744 621 225 106
Acc65I G'GTAC_C
Cuts at: 3752 6713 7434 3752
Size: 2961 721 6736
Fragments arranged by size:
6736 2961 721
AclI AA'CG_TT
Cuts at: 10258 10258
Size: 10418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2440 2730 3625 4429 5236 5929 6484 6943 8243
Size: 290 895 804 807 693 555 459 1300
Cuts at: 8243 8675 9422 9690 2440
Size: 432 747 268 3168
Fragments arranged by size:
3168 1300 895 807 804 747 693 555
459 432 290 268
AflIII A'CryG_T
Cuts at: 2591 5627 5705 5819 7770 8874 9851 2591
Size: 3036 78 114 1951 1104 977 3158
Fragments arranged by size:
3158 3036 1951 1104 977 114 78
AgeI A'CCGG_T
Cuts at: 5183 6929 8806 5183
Size: 1746 1877 6795
Fragments arranged by size:
6795 1877 1746
AleI CACnn'nnGTG
Cuts at: 2128 4732 7051 2128
Size: 2604 2319 5495
Fragments arranged by size:
5495 2604 2319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 941 2179 941
Size: 1238 9180
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4042 4074 7159 7191 4042
Size: 32 3085 32 7269
Fragments arranged by size:
7269 3085 32 32
AlwNI CAG_nnn'CTG
Cuts at: 135 486 1002 1776 4089 4854 5594 6921 7723
Size: 351 516 774 2313 765 740 1327 802
Cuts at: 7723 9290 135
Size: 1567 1263
Fragments arranged by size:
2313 1567 1327 1263 802 774 765 740
516 351
ApaI G_GGCC'C
Cuts at: 508 1240 2048 3796 3837 4153 4769 5250 7383
Size: 732 808 1748 41 316 616 481 2133
Cuts at: 7383 508
Size: 3543
Fragments arranged by size:
3543 2133 1748 808 732 616 481 316
41
ApaLI G'TGCA_C
Cuts at: 1456 1969 2347 2854 6861 9188 1456
Size: 513 378 507 4007 2327 2686
Fragments arranged by size:
4007 2686 2327 513 507 378
ApoI r'AATT_y
Cuts at: 7422 9771 10027 7422
Size: 2349 256 7813
Fragments arranged by size:
7813 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10418
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10418
AvaI C'yCGr_G
Cuts at: 466 507 516 667 834 1381 1684 6539 6596
Size: 41 9 151 167 547 303 4855 57
Cuts at: 6596 7327 7438 466
Size: 731 111 3446
Fragments arranged by size:
4855 3446 731 547 303 167 151 111
57 41 9
AvrII C'CTAG_G
Cuts at: 753 753
Size: 10418
BamHI G'GATC_C
Cuts at: 851 1808 2090 7443 851
Size: 957 282 5353 3826
Fragments arranged by size:
5353 3826 957 282
BanI G'GyrC_C
Cuts at: 282 511 1044 1153 3736 3752 3986 4723 4986
Size: 229 533 109 2583 16 234 737 263
Cuts at: 4986 5044 5078 5239 6658 6713 6899 7040 7434
Size: 58 34 161 1419 55 186 141 394
Cuts at: 7434 7848 8096 8131 282
Size: 414 248 35 2569
Fragments arranged by size:
2583 2569 1419 737 533 414 394 263
248 234 229 186 161 141 109 58
55 35 34 16
BanII G_rGCy'C
Cuts at: 172 460 467 508 590 743 835 885 965
Size: 288 7 41 82 153 92 50 80
Cuts at: 965 1240 1411 1504 2048 2692 3056 3443 3554
Size: 275 171 93 544 644 364 387 111
Cuts at: 3554 3796 3837 3860 3929 4153 4246 4769 5018
Size: 242 41 23 69 224 93 523 249
Cuts at: 5018 5250 5978 6362 6533 6615 6743 7304 7328
Size: 232 728 384 171 82 128 561 24
Cuts at: 7328 7383 7432 8462 172
Size: 55 49 1030 2128
Fragments arranged by size:
2128 1030 728 644 561 544 523 387
384 364 288 275 249 242 232 224
171 171 153 128 111 93 93 92
82 82 80 69 55 50 49 41
41 24 23 7
BbeI G_GCGC'C
Cuts at: 286 1048 7852 8100 286
Size: 762 6804 248 2604
Fragments arranged by size:
6804 2604 762 248
BbsI GAAGACnn'nnnn_
Cuts at: 539 2329 5532 539
Size: 1790 3203 5425
Fragments arranged by size:
5425 3203 1790
BbvCI CC'TCA_GC
Cuts at: 1253 4018 1253
Size: 2765 7653
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1525 1559 5463 5497 1525
Size: 34 3904 34 6446
Fragments arranged by size:
6446 3904 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1773 6316 8316 9077 10418 1773
Size: 4543 2000 761 1341 1773
Fragments arranged by size:
4543 2000 1773 1341 761
BclI T'GATC_A
Cuts at: 6950 7938 6950
Size: 988 9430
BfrBI ATG'CAT
Cuts at: 4009 4009
Size: 10418
BglI GCCn_nnn'nGGC
Cuts at: 335 758 2542 5866 335
Size: 423 1784 3324 4887
Fragments arranged by size:
4887 3324 1784 423
BglII A'GATC_T
Cuts at: 191 4487 7933 9970 191
Size: 4296 3446 2037 639
Fragments arranged by size:
4296 3446 2037 639
BlpI GC'TnA_GC
Cuts at: 959 1314 3136 3841 7267 7530 959
Size: 355 1822 705 3426 263 3847
Fragments arranged by size:
3847 3426 1822 705 355 263
Bme1580I G_kGCm'C
Cuts at: 331 508 514 1240 1460 1973 2048 2138 2351
Size: 177 6 726 220 513 75 90 213
Cuts at: 2351 2588 2858 2964 3266 3316 3739 3796 3837
Size: 237 270 106 302 50 423 57 41
Cuts at: 3837 4153 4769 5047 5081 5250 5476 6865 7311
Size: 316 616 278 34 169 226 1389 446
Cuts at: 7311 7383 8043 8136 9192 331
Size: 72 660 93 1056 1557
Fragments arranged by size:
1557 1389 1056 726 660 616 513 446
423 316 302 278 270 237 226 220
213 177 169 106 93 90 75 72
57 50 41 34 6
BmgBI CAC'GTC
Cuts at: 981 2142 5708 6204 6990 981
Size: 1161 3566 496 786 4409
Fragments arranged by size:
4409 3566 1161 786 496
BmrI ACTGGGnnnn_n'
Cuts at: 1226 1745 2361 3864 4200 4578 7630 7711 8046
Size: 519 616 1503 336 378 3052 81 335
Cuts at: 8046 10190 1226
Size: 2144 1454
Fragments arranged by size:
3052 2144 1503 1454 616 519 378 336
335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 7098 7161 8785 3407
Size: 3691 63 1624 5040
Fragments arranged by size:
5040 3691 1624 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 765 829 1636 1977 3457 3844 4012 4069
Size: 257 64 807 341 1480 387 168 57
Cuts at: 4069 4111 4855 6460 6637 6646 7231 508
Size: 42 744 1605 177 9 585 3695
Fragments arranged by size:
3695 1605 1480 807 744 585 387 341
257 177 168 64 57 42 9
Bpu10I CC'TnA_GC
Cuts at: 1253 2575 3392 4018 4780 1253
Size: 1322 817 626 762 6891
Fragments arranged by size:
6891 1322 817 762 626
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 1510 2293 3364 3688 3715 4048 4723 7015
Size: 1095 783 1071 324 27 333 675 2292
Cuts at: 7015 7051 7594 8473 8965 9263 415
Size: 36 543 879 492 298 1570
Fragments arranged by size:
2292 1570 1095 1071 879 783 675 543
492 333 324 298 36 27
BsaI GGTCTCn'nnnn_
Cuts at: 996 3025 3171 3912 10122 996
Size: 2029 146 741 6210 1292
Fragments arranged by size:
6210 2029 1292 741 146
BsaAI yAC'GTr
Cuts at: 1968 2163 2451 2583 2598 2837 5628 5820 7771
Size: 195 288 132 15 239 2791 192 1951
Cuts at: 7771 1968
Size: 4615
Fragments arranged by size:
4615 2791 1951 288 239 195 192 132
15
BsaBI GATnn'nnATC
Cuts at: 6 196 7932 9969 6
Size: 190 7736 2037 455
Fragments arranged by size:
7736 2037 455 190
BsaHI Gr'CG_yC
Cuts at: 283 292 1045 2813 4156 4231 4280 4673 4981
Size: 9 753 1768 1343 75 49 393 308
Cuts at: 4981 6104 6239 6368 7849 8097 8799 10295 283
Size: 1123 135 129 1481 248 702 1496 406
Fragments arranged by size:
1768 1496 1481 1343 1123 753 702 406
393 308 248 135 129 75 49 9
BsaWI w'CCGG_w
Cuts at: 773 965 5183 6929 7607 8128 8806 9080 9227
Size: 192 4218 1746 678 521 678 274 147
Cuts at: 9227 9638 773
Size: 411 1553
Fragments arranged by size:
4218 1746 1553 678 678 521 411 274
192 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 358 388 1393 1423 4042 4069 4072 4099 5770
Size: 30 1005 30 2619 27 3 27 1671
Cuts at: 5770 5800 7102 7132 358
Size: 30 1302 30 3644
Fragments arranged by size:
3644 2619 1671 1302 1005 30 30 30
30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 796 1023 1270 1441 1876 2626 3004 3043 3061
Size: 227 247 171 435 750 378 39 18
Cuts at: 3061 3064 3412 4090 4651 6859 7384 7387 796
Size: 3 348 678 561 2208 525 3 3827
Fragments arranged by size:
3827 2208 750 678 561 525 435 378
348 247 227 171 39 18 3 3
BseYI C'CCAG_C
Cuts at: 225 438 448 843 995 1002 1182 2916 3943
Size: 213 10 395 152 7 180 1734 1027
Cuts at: 3943 4601 4682 4761 4963 5062 5226 5806 6717
Size: 658 81 79 202 99 164 580 911
Cuts at: 6717 7128 7382 7843 8601 9178 9674 225
Size: 411 254 461 758 577 496 969
Fragments arranged by size:
1734 1027 969 911 758 658 580 577
496 461 411 395 254 213 202 180
164 152 99 81 79 10 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 807 1143 1663 2185 2401 3487 5010 5164
Size: 704 336 520 522 216 1086 1523 154
Cuts at: 5164 6502 7075 103
Size: 1338 573 3446
Fragments arranged by size:
3446 1523 1338 1086 704 573 522 520
336 216 154
BsiEI CG_ry'CG
Cuts at: 104 1515 2061 5169 6891 7960 8006 9214 104
Size: 1411 546 3108 1722 1069 46 1208 1308
Fragments arranged by size:
3108 1722 1411 1308 1208 1069 546 46
BsiHKAI G_wGCw'C
Cuts at: 467 590 965 1315 1411 1460 1973 2222 2303
Size: 123 375 350 96 49 513 249 81
Cuts at: 2303 2351 2858 3332 3443 3860 3929 4136 4195
Size: 48 507 474 111 417 69 207 59
Cuts at: 4195 4699 4739 5897 6230 6424 6533 6548 6865
Size: 504 40 1158 333 194 109 15 317
Cuts at: 6865 7079 7304 7432 8210 8400 9192 467
Size: 214 225 128 778 190 792 1693
Fragments arranged by size:
1693 1158 792 778 513 507 504 474
417 375 350 333 317 249 225 214
207 194 190 128 123 111 109 96
81 69 59 49 48 40 15
BsmBI CGTCTCn'nnnn_
Cuts at: 338 4950 6230 7216 338
Size: 4612 1280 986 3540
Fragments arranged by size:
4612 3540 1280 986
Bsp1286I G_dGCh'C
Cuts at: 172 331 460 467 508 514 590 743 835
Size: 159 129 7 41 6 76 153 92
Cuts at: 835 885 965 1240 1315 1411 1460 1504 1973
Size: 50 80 275 75 96 49 44 469
Cuts at: 1973 2048 2138 2222 2303 2351 2588 2692 2858
Size: 75 90 84 81 48 237 104 166
Cuts at: 2858 2964 3056 3266 3316 3332 3443 3554 3739
Size: 106 92 210 50 16 111 111 185
Cuts at: 3739 3796 3837 3860 3929 4136 4153 4195 4246
Size: 57 41 23 69 207 17 42 51
Cuts at: 4246 4699 4739 4769 5018 5047 5081 5250 5476
Size: 453 40 30 249 29 34 169 226
Cuts at: 5476 5897 5978 6230 6362 6424 6533 6548 6615
Size: 421 81 252 132 62 109 15 67
Cuts at: 6615 6743 6865 7079 7304 7311 7328 7383 7432
Size: 128 122 214 225 7 17 55 49
Cuts at: 7432 8043 8136 8210 8400 8462 9192 172
Size: 611 93 74 190 62 730 1398
Fragments arranged by size:
1398 730 611 469 453 421 275 252
249 237 226 225 214 210 207 190
185 169 166 159 153 132 129 128
122 111 111 109 106 104 96 93
92 92 90 84 81 81 80 76
75 75 74 69 67 62 62 57
55 51 50 50 49 49 48 44
42 41 41 40 34 30 29 23
17 17 16 15 7 7 6
BspEI T'CCGG_A
Cuts at: 773 965 9638 773
Size: 192 8673 1553
Fragments arranged by size:
8673 1553 192
BspHI T'CATG_A
Cuts at: 1463 9594 1463
Size: 8131 2287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 689 829 1091 1649 3071 3671 4738 4937 5038
Size: 140 262 558 1422 600 1067 199 101
Cuts at: 5038 5159 5378 5471 5585 5671 5702 6557 7104
Size: 121 219 93 114 86 31 855 547
Cuts at: 7104 7468 7682 7984 8365 689
Size: 364 214 302 381 2742
Fragments arranged by size:
2742 1422 1067 855 600 558 547 381
364 302 262 219 214 199 140 121
114 101 93 86 31
BsrBI CCG'CTC
Cuts at: 50 3723 6216 7038 7377 8710 8764 10248 10413
Size: 3673 2493 822 339 1333 54 1484 165
Cuts at: 10413 50
Size: 55
Fragments arranged by size:
3673 2493 1484 1333 822 339 165 55
54
BsrDI GCAATG_nn'
Cuts at: 3677 4009 8330 3677
Size: 332 4321 5765
Fragments arranged by size:
5765 4321 332
BsrFI r'CCGG_y
Cuts at: 298 1336 1719 2303 2371 2719 3017 3220 3338
Size: 1038 383 584 68 348 298 203 118
Cuts at: 3338 4652 5047 5128 5183 5523 5697 6409 6651
Size: 1314 395 81 55 340 174 712 242
Cuts at: 6651 6929 8416 8597 8806 298
Size: 278 1487 181 209 1910
Fragments arranged by size:
1910 1487 1314 1038 712 584 395 383
348 340 298 278 242 209 203 181
174 118 81 68 55
BsrGI T'GTAC_A
Cuts at: 6557 6557
Size: 10418
BssHII G'CGCG_C
Cuts at: 2109 3577 2109
Size: 1468 8950
BssSI C'ACGA_G
Cuts at: 1422 1453 4690 5545 8689 9047 1422
Size: 31 3237 855 3144 358 2793
Fragments arranged by size:
3237 3144 2793 855 358 31
BstAPI GCAn_nnn'nTGC
Cuts at: 126 135 1270 1641 2226 3327 6225 7795 126
Size: 9 1135 371 585 1101 2898 1570 2749
Fragments arranged by size:
2898 2749 1570 1135 1101 585 371 9
BstBI TT'CG_AA
Cuts at: 8779 8779
Size: 10418
BstXI CCAn_nnnn'nTGG
Cuts at: 1758 2805 3543 4527 4609 4976 5070 6186 6753
Size: 1047 738 984 82 367 94 1116 567
Cuts at: 6753 1758
Size: 5423
Fragments arranged by size:
5423 1116 1047 984 738 567 367 94
82
BstYI r'GATC_y
Cuts at: 1 191 535 851 1601 1808 1979 2018 2090
Size: 190 344 316 750 207 171 39 72
Cuts at: 2090 3290 3401 4487 6575 7443 7910 7933 8268
Size: 1200 111 1086 2088 868 467 23 335
Cuts at: 8268 8514 9526 9612 9624 9970 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2088 1200 1086 1012 868 750 467 449
346 344 335 316 246 207 190 171
111 86 72 39 23 12
BtgI C'CryG_G
Cuts at: 107 172 377 1054 2261 2327 2816 2956 3008
Size: 65 205 677 1207 66 489 140 52
Cuts at: 3008 3036 3503 3554 3693 4251 4425 4526 4608
Size: 28 467 51 139 558 174 101 82
Cuts at: 4608 5255 5864 5970 6075 6089 6692 7295 107
Size: 647 609 106 105 14 603 603 3230
Fragments arranged by size:
3230 1207 677 647 609 603 603 558
489 467 205 174 140 139 106 105
101 82 66 65 52 51 28 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2210 2637 3495 4202 5472 6684 8368 8549 8712
Size: 427 858 707 1270 1212 1684 181 163
Cuts at: 8712 2210
Size: 3916
Fragments arranged by size:
3916 1684 1270 1212 858 707 427 181
163
BtsI GCAGTG_nn'
Cuts at: 918 2896 2928 4449 5073 6514 7240 7661 918
Size: 1978 32 1521 624 1441 726 421 3675
Fragments arranged by size:
3675 1978 1521 1441 726 624 421 32
DraI TTT'AAA
Cuts at: 7417 7417
Size: 10418
DraIII CAC_nnn'GTG
Cuts at: 2142 2286 2355 3327 6459 2142
Size: 144 69 972 3132 6101
Fragments arranged by size:
6101 3132 972 144 69
DrdI GACnn_nn'nnGTC
Cuts at: 2818 7147 8124 8982 2818
Size: 4329 977 858 4254
Fragments arranged by size:
4329 4254 977 858
EaeI y'GGCC_r
Cuts at: 336 569 647 983 1164 1205 1283 2058 2264
Size: 233 78 336 181 41 78 775 206
Cuts at: 2264 2427 2489 2717 2953 3506 4374 4517 5130
Size: 163 62 228 236 553 868 143 613
Cuts at: 5130 5507 5525 5549 5867 6027 6086 6154 6206
Size: 377 18 24 318 160 59 68 52
Cuts at: 6206 6300 6311 6695 6872 8003 8177 8568 8595
Size: 94 11 384 177 1131 174 391 27
Cuts at: 8595 9940 336
Size: 1345 814
Fragments arranged by size:
1345 1131 868 814 775 613 553 391
384 377 336 318 236 233 228 206
181 177 174 163 160 143 94 78
78 68 62 59 52 41 27 24
18 11
EagI C'GGCC_G
Cuts at: 2058 8003 2058
Size: 5945 4473
EarI CTCTTCn'nnn_
Cuts at: 2473 3730 3985 4483 5161 5746 5848 5893 8441
Size: 1257 255 498 678 585 102 45 2548
Cuts at: 8441 8651 2473
Size: 210 4240
Fragments arranged by size:
4240 2548 1257 678 585 498 255 210
102 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1972 2011 2305 2943 3237 4159 5802 8626 8936
Size: 39 294 638 294 922 1643 2824 310
Cuts at: 8936 9082 10228 1972
Size: 146 1146 2162
Fragments arranged by size:
2824 2162 1643 1146 922 638 310 294
294 146 39
EcoICRI GAG'CTC
Cuts at: 465 588 963 1409 3441 3858 3927 6531 7302
Size: 123 375 446 2032 417 69 2604 771
Cuts at: 7302 7430 465
Size: 128 3453
Fragments arranged by size:
3453 2604 2032 771 446 417 375 128
123 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 765 829 1636 1977 2440 2730 3457 3625
Size: 257 64 807 341 463 290 727 168
Cuts at: 3625 3844 4012 4069 4111 4429 4855 5236 5929
Size: 219 168 57 42 318 426 381 693
Cuts at: 5929 6460 6484 6637 6646 6943 7231 8243 8675
Size: 531 24 153 9 297 288 1012 432
Cuts at: 8675 9422 9690 508
Size: 747 268 1236
Fragments arranged by size:
1236 1012 807 747 727 693 531 463
432 426 381 341 318 297 290 288
268 257 219 168 168 153 64 57
42 24 9
EcoNI CCTnn'n_nnAGG
Cuts at: 727 1618 1729 1888 4342 5467 6274 727
Size: 891 111 159 2454 1125 807 4871
Fragments arranged by size:
4871 2454 1125 891 807 159 111
EcoO109I rG'GnC_Cy
Cuts at: 177 471 492 1080 1237 3432 3594 3792 3793
Size: 294 21 588 157 2195 162 198 1
Cuts at: 3793 3834 4149 4320 4359 4765 4926 5246 5247
Size: 41 315 171 39 406 161 320 1
Cuts at: 5247 5802 5904 7332 7353 7557 177
Size: 555 102 1428 21 204 3038
Fragments arranged by size:
3038 2195 1428 588 555 406 320 315
294 204 198 171 162 161 157 102
41 39 21 21 1 1
EcoRI G'AATT_C
Cuts at: 7422 7422
Size: 10418
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 285 4494 8437 285
Size: 4209 3943 2266
Fragments arranged by size:
4209 3943 2266
FseI GG_CCGG'CC
Cuts at: 1340 1340
Size: 10418
FspI TGC'GCA
Cuts at: 5039 5278 8199 5039
Size: 239 2921 7258
Fragments arranged by size:
7258 2921 239
HaeII r_GCGC'y
Cuts at: 286 402 942 1048 1072 1390 3024 3978 4644
Size: 116 540 106 24 318 1634 954 666
Cuts at: 4644 5444 6774 6859 7852 8100 9122 10101 286
Size: 800 1330 85 993 248 1022 979 603
Fragments arranged by size:
1634 1330 1022 993 979 954 800 666
603 540 318 248 116 106 85 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 700 732 1108 1140 1726 1758 4069 4101 4348
Size: 32 376 32 586 32 2311 32 247
Cuts at: 4348 4380 4539 4571 7102 7134 7219 7251 8260
Size: 32 159 32 2531 32 85 32 1009
Cuts at: 8260 8290 8292 8322 700
Size: 30 2 30 2796
Fragments arranged by size:
2796 2531 2311 1009 586 376 247 159
85 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1512 1875 5919 6318 6372 7457 1512
Size: 363 4044 399 54 1085 4473
Fragments arranged by size:
4473 4044 1085 399 363 54
KasI G'GCGC_C
Cuts at: 282 1044 7848 8096 282
Size: 762 6804 248 2604
Fragments arranged by size:
6804 2604 762 248
KpnI G_GTAC'C
Cuts at: 3756 6717 7438 3756
Size: 2961 721 6736
Fragments arranged by size:
6736 2961 721
MluI A'CGCG_T
Cuts at: 9851 9851
Size: 10418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3259 3470 4052 5189 5199 7052 9089 9273 3259
Size: 211 582 1137 10 1853 2037 184 4404
Fragments arranged by size:
4404 2037 1853 1137 582 211 184 10
MscI TGG'CCA
Cuts at: 571 985 1285 2266 2491 3508 4519 5509 5869
Size: 414 300 981 225 1017 1011 990 360
Cuts at: 5869 6029 6088 6208 6874 8179 571
Size: 160 59 120 666 1305 2810
Fragments arranged by size:
2810 1305 1017 1011 990 981 666 414
360 300 225 160 120 59
MslI CAynn'nnrTG
Cuts at: 256 2128 2395 2497 2596 2803 3205 3280 3650
Size: 1872 267 102 99 207 402 75 370
Cuts at: 3650 4525 4732 5186 5503 5983 6169 6751 6880
Size: 875 207 454 317 480 186 582 129
Cuts at: 6880 7051 8407 8534 256
Size: 171 1356 127 2140
Fragments arranged by size:
2140 1872 1356 875 582 480 454 402
370 317 267 207 207 186 171 129
127 102 99 75
MspA1I CmG'CkG
Cuts at: 126 132 280 390 975 1002 1252 1437 1776
Size: 6 148 110 585 27 250 185 339
Cuts at: 1776 2232 2385 3840 3848 4566 4787 4963 5024
Size: 456 153 1455 8 718 221 176 61
Cuts at: 5024 5591 6077 6555 6668 7386 7529 7843 8203
Size: 567 486 478 113 718 143 314 360
Cuts at: 8203 9216 9461 126
Size: 1013 245 1083
Fragments arranged by size:
1455 1083 1013 718 718 585 567 486
478 456 360 339 314 250 245 221
185 176 153 148 143 113 110 61
27 8 6
NaeI GCC'GGC
Cuts at: 300 1338 1721 2721 3019 5525 5699 6653 8599
Size: 1038 383 1000 298 2506 174 954 1946
Cuts at: 8599 300
Size: 2119
Fragments arranged by size:
2506 2119 1946 1038 1000 954 383 298
174
NarI GG'CG_CC
Cuts at: 283 1045 7849 8097 283
Size: 762 6804 248 2604
Fragments arranged by size:
6804 2604 762 248
NcoI C'CATG_G
Cuts at: 107 2327 3008 3693 5255 6089 107
Size: 2220 681 685 1562 834 4436
Fragments arranged by size:
4436 2220 1562 834 685 681
NgoMIV G'CCGG_C
Cuts at: 298 1336 1719 2719 3017 5523 5697 6651 8597
Size: 1038 383 1000 298 2506 174 954 1946
Cuts at: 8597 298
Size: 2119
Fragments arranged by size:
2506 2119 1946 1038 1000 954 383 298
174
NotI GC'GGCC_GC
Cuts at: 2058 2058
Size: 10418
NsiI A_TGCA'T
Cuts at: 4011 4011
Size: 10418
NspI r_CATG'y
Cuts at: 2595 2868 3312 3889 4830 5325 5640 6168 7471
Size: 273 444 577 941 495 315 528 1303
Cuts at: 7471 7971 8878 2595
Size: 500 907 4135
Fragments arranged by size:
4135 1303 941 907 577 528 500 495
444 315 273
PasI CC'CwG_GG
Cuts at: 1149 1413 4138 1149
Size: 264 2725 7429
Fragments arranged by size:
7429 2725 264
PciI A'CATG_T
Cuts at: 2591 8874 2591
Size: 6283 4135
PflMI CCAn_nnn'nTGG
Cuts at: 486 1002 1189 3648 486
Size: 516 187 2459 7256
Fragments arranged by size:
7256 2459 516 187
PfoI T'CCnGG_A
Cuts at: 806 1613 2093 3542 4120 4541 6289 6580 6731
Size: 807 480 1449 578 421 1748 291 151
Cuts at: 6731 7016 806
Size: 285 4208
Fragments arranged by size:
4208 1748 1449 807 578 480 421 291
285 151
PmeI GTTT'AAAC
Cuts at: 7417 7417
Size: 10418
PmlI CAC'GTG
Cuts at: 1968 2451 2837 1968
Size: 483 386 9549
Fragments arranged by size:
9549 483 386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4042 4074 9557 9589 4042
Size: 32 5483 32 4871
Fragments arranged by size:
5483 4871 32 32
PpuMI rG'GwC_Cy
Cuts at: 177 1080 3432 3594 4320 4926 5802 5904 7332
Size: 903 2352 162 726 606 876 102 1428
Cuts at: 7332 177
Size: 3263
Fragments arranged by size:
3263 2352 1428 903 876 726 606 162
102
PshAI GACnn'nnGTC
Cuts at: 6277 6277
Size: 10418
PspOMI G'GGCC_C
Cuts at: 504 1236 2044 3792 3833 4149 4765 5246 7379
Size: 732 808 1748 41 316 616 481 2133
Cuts at: 7379 504
Size: 3543
Fragments arranged by size:
3543 2133 1748 808 732 616 481 316
41
PspXI vC'TCGA_Gb
Cuts at: 466 466
Size: 10418
PstI C_TGCA'G
Cuts at: 131 365 890 913 1067 1109 1278 1304 1346
Size: 234 525 23 154 42 169 26 42
Cuts at: 1346 1634 1655 1772 2903 4226 4934 5471 5582
Size: 288 21 117 1131 1323 708 537 111
Cuts at: 5582 6554 7247 7465 8150 131
Size: 972 693 218 685 2399
Fragments arranged by size:
2399 1323 1131 972 708 693 685 537
525 288 234 218 169 154 117 111
42 42 26 23 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10418
PvuII CAG'CTG
Cuts at: 126 132 390 975 1002 1776 2385 3848 6555
Size: 6 258 585 27 774 609 1463 2707
Cuts at: 6555 7386 7843 8203 126
Size: 831 457 360 2341
Fragments arranged by size:
2707 2341 1463 831 774 609 585 457
360 258 27 6
RsrII CG'GwC_CG
Cuts at: 8613 8613
Size: 10418
SacI G_AGCT'C
Cuts at: 467 590 965 1411 3443 3860 3929 6533 7304
Size: 123 375 446 2032 417 69 2604 771
Cuts at: 7304 7432 467
Size: 128 3453
Fragments arranged by size:
3453 2604 2032 771 446 417 375 128
123 69
SacII CC_GC'GG
Cuts at: 6078 6078
Size: 10418
SalI G'TCGA_C
Cuts at: 1510 7455 1510
Size: 5945 4473
SapI GCTCTTCn'nnn_
Cuts at: 2473 3730 5161 5893 8441 8651 2473
Size: 1257 1431 732 2548 210 4240
Fragments arranged by size:
4240 2548 1431 1257 732 210
SbfI CC_TGCA'GG
Cuts at: 890 4934 7465 890
Size: 4044 2531 3843
Fragments arranged by size:
4044 3843 2531
ScaI AGT'ACT
Cuts at: 2698 6013 9935 2698
Size: 3315 3922 3181
Fragments arranged by size:
3922 3315 3181
SexAI A'CCwGG_T
Cuts at: 3158 5648 3158
Size: 2490 7928
SfcI C'TryA_G
Cuts at: 33 127 361 886 909 1063 1105 1274 1300
Size: 94 234 525 23 154 42 169 26
Cuts at: 1300 1342 1630 1651 1768 2899 4222 4930 5467
Size: 42 288 21 117 1131 1323 708 537
Cuts at: 5467 5578 5881 6550 6663 7243 7461 8146 9139
Size: 111 303 669 113 580 218 685 993
Cuts at: 9139 9330 33
Size: 191 1121
Fragments arranged by size:
1323 1131 1121 993 708 685 669 580
537 525 303 288 234 218 191 169
154 117 113 111 94 42 42 26
23 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 2542 2542
Size: 10418
SfoI GGC'GCC
Cuts at: 284 1046 7850 8098 284
Size: 762 6804 248 2604
Fragments arranged by size:
6804 2604 762 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10418
SmaI CCC'GGG
Cuts at: 509 6541 6598 7440 509
Size: 6032 57 842 3487
Fragments arranged by size:
6032 3487 842 57
SmlI C'TyrA_G
Cuts at: 394 466 1525 2308 3379 3703 3730 4063 4738
Size: 72 1059 783 1071 324 27 333 675
Cuts at: 4738 7030 7066 7573 8488 8980 9242 394
Size: 2292 36 507 915 492 262 1570
Fragments arranged by size:
2292 1570 1071 1059 915 783 675 507
492 333 324 262 72 36 27
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10418
SphI G_CATG'C
Cuts at: 5325 6168 7471 7971 5325
Size: 843 1303 500 7772
Fragments arranged by size:
7772 1303 843 500
SrfI GCCC'GGGC
Cuts at: 509 509
Size: 10418
StuI AGG'CCT
Cuts at: 1894 3001 4936 6439 1894
Size: 1107 1935 1503 5873
Fragments arranged by size:
5873 1935 1503 1107
StyI C'CwwG_G
Cuts at: 107 753 986 1823 2327 2531 3008 3095 3693
Size: 646 233 837 504 204 477 87 598
Cuts at: 3693 4469 5255 5564 6089 6275 6761 7552 7906
Size: 776 786 309 525 186 486 791 354
Cuts at: 7906 107
Size: 2619
Fragments arranged by size:
2619 837 791 786 776 646 598 525
504 486 477 354 309 233 204 186
87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2343 2873 3073 3655 4615 4711 5134 5268 5744
Size: 530 200 582 960 96 423 134 476
Cuts at: 5744 6322 7137 8001 8134 8593 8802 2343
Size: 578 815 864 133 459 209 3959
Fragments arranged by size:
3959 960 864 815 582 578 530 476
459 423 209 200 134 133 96
TatI w'GTAC_w
Cuts at: 2525 2696 3608 5999 6011 6137 6557 9933 2525
Size: 171 912 2391 12 126 420 3376 3010
Fragments arranged by size:
3376 3010 2391 912 420 171 126 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4387 4419 6443 6475 9935 9967 4387
Size: 32 2024 32 3460 32 4838
Fragments arranged by size:
4838 3460 2024 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1516 1906 3474 4206 4330 6067 6253 8215 1516
Size: 390 1568 732 124 1737 186 1962 3719
Fragments arranged by size:
3719 1962 1737 1568 732 390 186 124
XbaI T'CTAG_A
Cuts at: 7449 7449
Size: 10418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 685 1830 2538 2805 3516 3561 3645 4758 5451
Size: 1145 708 267 711 45 84 1113 693
Cuts at: 5451 5961 6750 7913 685
Size: 510 789 1163 3190
Fragments arranged by size:
3190 1163 1145 1113 789 711 708 693
510 267 84 45
XhoI C'TCGA_G
Cuts at: 466 466
Size: 10418
XmaI C'CCGG_G
Cuts at: 507 6539 6596 7438 507
Size: 6032 57 842 3487
Fragments arranged by size:
6032 3487 842 57
ZraI GAC'GTC
Cuts at: 293 4281 4982 293
Size: 3988 701 5729
Fragments arranged by size:
5729 3988 701
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI BaeI BmtI BsiWI BsmI
BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NruI PacI PsiI PsrI
SanDI SgrAI SnaBI SspI SwaI XmnI