Restriction map for pF1KA1016
 (Linear) (Six-base) MAPSORT of: KIAA1016.seq  Check: 5671  from: 1  to: 2184



KIAA1016.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AccI GT'mk_AC

Cuts at:      0    1791    2184 
   Size:       1791     393
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1660    2184 
   Size:       1660     524
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     748     840    2184 
   Size:        748      92    1344

  Fragments arranged by size: 

                1344     748      92
 
AlwNI CAG_nnn'CTG

Cuts at:      0     432    1981    2184 
   Size:        432    1549     203

  Fragments arranged by size: 

                1549     432     203
 
ApaLI G'TGCA_C

Cuts at:      0    1291    2184 
   Size:       1291     893
 
ApoI r'AATT_y

Cuts at:      0     253     368    1123    1135    1228    1674    2184 
   Size:        253     115     755      12      93     446     510

  Fragments arranged by size: 

                 755     510     446     253     115      93      12
 
AvaI C'yCGr_G

Cuts at:      0     274    2059    2184 
   Size:        274    1785     125

  Fragments arranged by size: 

                1785     274     125
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     375     408    2184 
   Size:        375      33    1776

  Fragments arranged by size: 

                1776     375      33
 
BanI G'GyrC_C

Cuts at:      0     118    2184 
   Size:        118    2066
 
BbeI G_GCGC'C

Cuts at:      0     122    2184 
   Size:        122    2062
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1031    1262    1805    2184 
   Size:       1031     231     543     379

  Fragments arranged by size: 

                1031     543     379     231
 
BglII A'GATC_T

Cuts at:      0     693    2184 
   Size:        693    1491
 
Bme1580I G_kGCm'C

Cuts at:      0    1295    2184 
   Size:       1295     889
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     797    1525    2082    2184 
   Size:        208     589     728     557     102

  Fragments arranged by size: 

                 728     589     557     208     102
 
Bpu10I CC'TnA_GC

Cuts at:      0     232    1105    2184 
   Size:        232     873    1079

  Fragments arranged by size: 

                1079     873     232
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     468     808    2184 
   Size:        220     248     340    1376

  Fragments arranged by size: 

                1376     340     248     220
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1184    1222    2184 
   Size:       1184      38     962

  Fragments arranged by size: 

                1184     962      38
 
BsaAI yAC'GTr

Cuts at:      0     939    2184 
   Size:        939    1245
 
BsaHI Gr'CG_yC

Cuts at:      0       7     119    2184 
   Size:          7     112    2065

  Fragments arranged by size: 

                2065     112       7
 
BsaWI w'CCGG_w

Cuts at:      0    1143    1515    1706    2184 
   Size:       1143     372     191     478

  Fragments arranged by size: 

                1143     478     372     191
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1078    1108    2184 
   Size:       1078      30    1076

  Fragments arranged by size: 

                1078    1076      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     217     800    2184 
   Size:        217     583    1384

  Fragments arranged by size: 

                1384     583     217
 
BseYI C'CCAG_C

Cuts at:      0     611    2184 
   Size:        611    1573
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     319    1466    2184 
   Size:         43     276    1147     718

  Fragments arranged by size: 

                1147     718     276      43
 
BsiEI CG_ry'CG

Cuts at:      0    1867    2184 
   Size:       1867     317
 
BsiHKAI G_wGCw'C

Cuts at:      0     737    1295    2184 
   Size:        737     558     889

  Fragments arranged by size: 

                 889     737     558
 
BsmI GAATG_Cn'

Cuts at:      0    2175    2184 
   Size:       2175       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     737    1295    2184 
   Size:        737     558     889

  Fragments arranged by size: 

                 889     737     558
 
BspEI T'CCGG_A

Cuts at:      0    1706    2184 
   Size:       1706     478
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     176    2184 
   Size:        176    2008
 
BsrBI CCG'CTC

Cuts at:      0    2096    2184 
   Size:       2096      88
 
BsrDI GCAATG_nn'

Cuts at:      0    1355    2184 
   Size:       1355     829
 
BsrFI r'CCGG_y

Cuts at:      0     115    2184 
   Size:        115    2069
 
BssHII G'CGCG_C

Cuts at:      0     192     194    2184 
   Size:        192       2    1990

  Fragments arranged by size: 

                1990     192       2
 
BssSI C'ACGA_G

Cuts at:      0     736    1092    2184 
   Size:        736     356    1092

  Fragments arranged by size: 

                1092     736     356
 
BstBI TT'CG_AA

Cuts at:      0    2031    2184 
   Size:       2031     153
 
BstYI r'GATC_y

Cuts at:      0     693    1389    2184 
   Size:        693     696     795

  Fragments arranged by size: 

                 795     696     693
 
Bsu36I CC'TnA_GG

Cuts at:      0     407    2184 
   Size:        407    1777
 
BtgI C'CryG_G

Cuts at:      0     105     911    1861    2184 
   Size:        105     806     950     323

  Fragments arranged by size: 

                 950     806     323     105
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1084    2184 
   Size:       1084    1100
 
DraI TTT'AAA

Cuts at:      0    1433    2184 
   Size:       1433     751
 
EaeI y'GGCC_r

Cuts at:      0     209    1916    2184 
   Size:        209    1707     268

  Fragments arranged by size: 

                1707     268     209
 
EarI CTCTTCn'nnn_

Cuts at:      0     539    1259    1277    1727    2184 
   Size:        539     720      18     450     457

  Fragments arranged by size: 

                 720     539     457     450      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     456    1846    2184 
   Size:        456    1390     338

  Fragments arranged by size: 

                1390     456     338
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     797    1525    1660    2082    2184 
   Size:        208     589     728     135     422     102

  Fragments arranged by size: 

                 728     589     422     208     135     102
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2103    2184 
   Size:       2103      81
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     732    2184 
   Size:        732    1452
 
HaeII r_GCGC'y

Cuts at:      0     122     239    2184 
   Size:        122     117    1945

  Fragments arranged by size: 

                1945     122     117
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     441     473    1769    1801    1975    2007    2184 
   Size:        441      32    1296      32     174      32     177

  Fragments arranged by size: 

                1296     441     177     174      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1848    2043    2184 
   Size:       1848     195     141

  Fragments arranged by size: 

                1848     195     141
 
KasI G'GCGC_C

Cuts at:      0     118    2184 
   Size:        118    2066
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     954    1994    2184 
   Size:        954    1040     190

  Fragments arranged by size: 

                1040     954     190
 
MscI TGG'CCA

Cuts at:      0    1918    2184 
   Size:       1918     266
 
MslI CAynn'nnrTG

Cuts at:      0    1771    2168    2184 
   Size:       1771     397      16

  Fragments arranged by size: 

                1771     397      16
 
MspA1I CmG'CkG

Cuts at:      0     155     462     587     771     889    1362    1503    1897 
   Size:        155     307     125     184     118     473     141     394

Cuts at:   1897    2184 
   Size:        287

  Fragments arranged by size: 

                 473     394     307     287     184     155     141     125
                 118
 
NarI GG'CG_CC

Cuts at:      0     119    2184 
   Size:        119    2065
 
NcoI C'CATG_G

Cuts at:      0     911    2184 
   Size:        911    1273
 
NspI r_CATG'y

Cuts at:      0     875    2184 
   Size:        875    1309
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     912     939    2184 
   Size:        912      27    1245

  Fragments arranged by size: 

                1245     912      27
 
PmlI CAC'GTG

Cuts at:      0     939    2184 
   Size:        939    1245
 
PpuMI rG'GwC_Cy

Cuts at:      0    2103    2184 
   Size:       2103      81
 
PstI C_TGCA'G

Cuts at:      0     428     806    1602    2012    2184 
   Size:        428     378     796     410     172

  Fragments arranged by size: 

                 796     428     410     378     172
 
PvuII CAG'CTG

Cuts at:      0     462     587     771     889    1362    1503    2184 
   Size:        462     125     184     118     473     141     681

  Fragments arranged by size: 

                 681     473     462     184     141     125     118
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1259    2184 
   Size:       1259     925
 
SfcI C'TryA_G

Cuts at:      0     424     802    1598    1685    2008    2184 
   Size:        424     378     796      87     323     176

  Fragments arranged by size: 

                 796     424     378     323     176      87
 
SfoI GGC'GCC

Cuts at:      0     120    2184 
   Size:        120    2064
 
SmaI CCC'GGG

Cuts at:      0     276    2184 
   Size:        276    1908
 
SmlI C'TyrA_G

Cuts at:      0     199     447     787    2184 
   Size:        199     248     340    1397

  Fragments arranged by size: 

                1397     340     248     199
 
SpeI A'CTAG_T

Cuts at:      0     687    2184 
   Size:        687    1497
 
SphI G_CATG'C

Cuts at:      0     875    2184 
   Size:        875    1309
 
StyI C'CwwG_G

Cuts at:      0     701     911    2184 
   Size:        701     210    1273

  Fragments arranged by size: 

                1273     701     210
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      77    1150    2184 
   Size:         77    1073    1034

  Fragments arranged by size: 

                1073    1034      77
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1078    1110    2184 
   Size:       1078      32    1074

  Fragments arranged by size: 

                1078    1074      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1911    2184 
   Size:       1911     273
 
XmaI C'CCGG_G

Cuts at:      0     274    2184 
   Size:        274    1910
 
XmnI GAAnn'nnTTC

Cuts at:      0    1754    2184 
   Size:       1754     430
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI    ApaLI     ApoI     AvaI     BaeI
     BanI     BbeI     BbsI    BglII Bme1580I     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI    BstBI    BstYI   Bsu36I     BtgI    BtgZI
     DraI     EaeI     EarI     EciI  Eco57MI EcoO109I     FalI    HaeII
    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NspI    PflMI     PmlI    PpuMI     PstI    PvuII     SapI
     SfcI     SfoI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI     AscI     AseI    AsiSI    AvrII
    BamHI    BanII    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI    BmgBI     BmrI     BmtI     BplI    BsaBI    BsiWI    BsmBI
    BspHI    BsrGI   BstAPI   BstEII    BstXI  BstZ17I     BtsI     ClaI
    CspCI   DraIII     DrdI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XhoI     ZraI
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