Restriction map for pF1KA0982
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

Acc65I      AccI        AccIII      AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BanI        BbeI        BbvCI       BciVI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaI        BseAI       BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MluI        MroI        MslI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       PstI        PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII         933  GACGTC               GACGT^C
AclI          263  AACGTT               AA^CGTT
AcyI          933  GACGTC               G[GA]^CG[CT]C
AflIII        122  ACATGT               A^C[GA][CT]GT
AlwNI         358  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      1080  GAATGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          514  CTCGGG               C^[CT]CG[GA]G
BalI          491  TGGCCA               TGG^CCA
BbrPI         592  CACGTG               CAC^GTG
BcgI           16  GCAAGTTGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI         933  GACGTC               G[GA]^CG[CT]C
BsaWI         905  ACCGGA               [AT]^CCGG[AT]
BseRI        1020  CTCCTC               GAGGAG(10/8)
BsmBI         931  GAGACG               CGTCTC(1/5)
BsoBI         514  CTCGGG               C^[CT]CG[GA]G
BspLU11I      122  ACATGT               A^CATGT
BsrFI        1430  ACCGGC               [GA]^CCGG[CT]
BstEII       1014  GGTGACC              G^GT[ACGT]ACC
BstPI        1014  GGTGACC              G^GT[ACGT]ACC
BstYI        1195  AGATCC               [GA]^GATC[CT]
Cfr10I       1430  ACCGGC               [GA]^CCGG[CT]
DraII        1058  GGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI         1127  CCGTGG               C^C[GA][CT]GG
EcoO109I     1058  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I      1014  GGTGACC              G^GT[ACGT]ACC
Hin1I         933  GACGTC               G[GA]^CG[CT]C
HincII        975  GTTGAC               GT[CT]^[GA]AC
HindII        975  GTTGAC               GT[CT]^[GA]AC
MflI         1195  AGATCC               [GA]^GATC[CT]
MluNI         491  TGGCCA               TGG^CCA
MscI          491  TGGCCA               TGG^CCA
NspI          122  ACATGT               [GA]CATG^[CT]
PmaCI         592  CACGTG               CAC^GTG
PmlI          592  CACGTG               CAC^GTG
PpuMI        1058  GGGACCC              [GA]G^G[AT]CC[CT]
Psp1406I      263  AACGTT               AA^CGTT
PvuII         691  CAGCTG               CAG^CTG
SacI          940  GAGCTC               GAGCT^C
SmlI          829  CTCAAG               C^T[CT][GA]AG
SnaBI         236  TACGTA               TAC^GTA
SpeI          721  ACTAGT               A^CTAGT
TfiI          139  GATTC                G^A[AT]TC
XhoII        1195  AGATCC               [GA]^GATC[CT]
XmnI         1080  GAATGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwI         1196  GATCC                GGATC(4/5)
             1238  GGATC               
AvaII         903  GGACC                G^G[AT]CC
             1059  GGACC               
BbsI          366  GAAGAC               GAAGAC(2/6)
             1390  GAAGAC              
BcnI          465  CCGGG                CC^[GC]GG
             1337  CCCGG               
BfaI          574  CTAG                 C^TAG
              722  CTAG                
BpuAI         366  GAAGAC               GAAGAC(2/6)
             1390  GAAGAC              
BsaAI         236  TACGTA               [CT]AC^GT[GA]
              592  CACGTG              
BsgI          987  CTGCAC               GTGCAG(16/14)
              688  GTGCAG              
BsrDI        1203  CATTGC               GCAATG(2/0)
             1466  CATTGC              
BssSI         442  CTCGTG               CACGAG(-5/-1)
             1009  CTCGTG              
DraIII       1072  CACTCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1396  CACCCCGTG           
HgaI          978  GACGC                GACGC(5/10)
              812  GCGTC               
MaeI          574  CTAG                 C^TAG
              722  CTAG                
MspA1I        691  CAGCTG               C[AC]G^C[GT]G
             1003  CAGCGG              
NciI          465  CCGGG                CC^[GC]GG
             1337  CCCGG               
PflMI         840  CCAGGGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1439  CCATTGGTTGG         
PleI          796  GACTC                GAGTC(4/5)
             1222  GACTC               
SfaNI         473  GCATC                GCATC(5/9)
              523  GCATC               
Van91I        840  CCAGGGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1439  CCATTGGTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
ApoI        AspHI       BanII       BbvI        BglI        
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EaeI        
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspI        MvaI        MvnI        
MwoI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com