Restriction map for pF1KA0968
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       AccI        AccII       AclI        AcsI        
AflIII      AgeI        AhdI        Aor51HI     ApaI        
ApoI        AscI        AseI        AsnI        AspEI       
AviII       AvrII       BamHI       BanII       BbrPI       
BbvCI       BcgI        BfaI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsiEI       BsmBI       
BsmI        BspHI       BspLU11I    BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstZ17I     CelII       CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoT22I     FseI        
FspI        HincII      HindII      HindIII     HpaI        
KspI        MaeI        MaeII       MamI        MfeI        
MluI        MunI        MvnI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SfcI        SfiI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        StuI        SwaI        
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I        626  GGTACC               G^GTACC
AccIII        940  TCCGGA               T^CCGGA
AflII         796  CTTAAG               C^TTAAG
AocI         1144  CCTGAGG              CC^T[ACGT]AGG
Asp700I       870  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       626  GGTACC               G^GTACC
AvaI          308  CCCGGG               C^[CT]CG[GA]G
BbsI         1021  GAAGAC               GAAGAC(2/6)
BciVI         344  GTATCC               GTATCC(6/5)
BclI          740  TGATCA               T^GATCA
BfrI          796  CTTAAG               C^TTAAG
BglI         1370  GCCGGGACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1405  AGATCT               A^GATCT
BpuAI        1021  GAAGAC               GAAGAC(2/6)
BsaWI         940  TCCGGA               [AT]^CCGG[AT]
BseAI         940  TCCGGA               T^CCGGA
BsiWI         566  CGTACG               C^GTACG
BsoBI         308  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      608  TGTACA               T^GTACA
BspDI        1184  ATCGAT               AT^CGAT
BspEI         940  TCCGGA               T^CCGGA
BspMI          13  ACCTGC               ACCTGC(4/8)
BsrBI         819  CCGCTC               CCGCTC(-3/-3)
BsrDI        1085  GCAATG               GCAATG(2/0)
BsrFI         679  GCCGGC               [GA]^CCGG[CT]
BsrGI         608  TGTACA               T^GTACA
BstBI        1138  TTCGAA               TT^CGAA
Bsu36I       1144  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        679  GCCGGC               [GA]^CCGG[CT]
ClaI         1184  ATCGAT               AT^CGAT
Eco81I       1144  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1161  CCTGGTTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       731  CCAAGG               C^C[AT][AT]GG
FbaI          740  TGATCA               T^GATCA
HgaI         1315  GACGC                GACGC(5/10)
KpnI          626  GGTACC               GGTAC^C
MroI          940  TCCGGA               T^CCGGA
NaeI          679  GCCGGC               GCC^GGC
NgoMIV        679  GCCGGC               G^CCGGC
NspI          839  GCATGC               [GA]CATG^[CT]
NspV         1138  TTCGAA               TT^CGAA
PshAI        1353  GACCCGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI         1138  TTCGAA               TT^CGAA
SmaI          308  CCCGGG               CCC^GGG
SphI          839  GCATGC               GCATG^C
SspBI         608  TGTACA               T^GTACA
StyI          731  CCAAGG               C^C[AT][AT]GG
XcmI          697  CCATCGCCGGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         308  CCCGGG               C^CCGGG
XmaI          308  CCCGGG               C^CCGGG
XmnI          870  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcyI          682  GGCGCC               G[GA]^CG[CT]C
             1413  GGCGCC              
Alw44I        394  GTGCAC               G^TGCAC
             1228  GTGCAC              
ApaLI         394  GTGCAC               G^TGCAC
             1228  GTGCAC              
AspI          268  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              712  GACACTGTC           
BbeI          682  GGCGCC               GGCGC^C
             1413  GGCGCC              
BpmI          169  CTGGAG               CTGGAG(16/14)
              358  CTGGAG              
BsaHI         682  GGCGCC               G[GA]^CG[CT]C
             1413  GGCGCC              
BseRI         241  GAGGAG               GAGGAG(10/8)
             1348  GAGGAG              
BsgI           15  CTGCAC               GTGCAG(16/14)
              370  CTGCAC              
CfoI          683  GCGC                 GCG^C
             1414  GCGC                
DsaI          827  CCGTGG               C^C[GA][CT]GG
              857  CCGTGG              
EcoRV         298  GATATC               GAT^ATC
              533  GATATC              
FauI           20  CCCGC                CCCGC(4/6)
              182  CCCGC               
HaeII         682  GGCGCC               [GA]GCGC^[CT]
             1413  GGCGCC              
HhaI          683  GCGC                 GCG^C
             1414  GCGC                
Hin1I         682  GGCGCC               G[GA]^CG[CT]C
             1413  GGCGCC              
HinP1I        683  GCGC                 G^CGC
             1414  GCGC                
KasI          682  GGCGCC               G^GCGCC
             1413  GGCGCC              
MseI          761  TTAA                 T^TAA
              797  TTAA                
MspA1I        782  CAGCTG               C[AC]G^C[GT]G
             1066  CAGCTG              
NarI          682  GGCGCC               GG^CGCC
             1413  GGCGCC              
PflFI         268  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              712  GACACTGTC           
PflMI         378  CCACCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1257  CCACCTGATGG         
PpuMI         401  GGGACCT              [GA]G^G[AT]CC[CT]
              560  AGGACCC             
PvuII         782  CAGCTG               CAG^CTG
             1066  CAGCTG              
SapI          966  GAAGAGC              GCTCTTC(1/4)
               42  GCTCTTC             
SexAI         269  ACCTGGT              A^CC[AT]GGT
             1160  ACCTGGT             
SfoI          682  GGCGCC               GGC^GCC
             1413  GGCGCC              
SmlI          439  CTCAAG               C^T[CT][GA]AG
              796  CTTAAG              
Tru9I         761  TTAA                 T^TAA
              797  TTAA                
Tsp509I        53  AATT                 ^AATT
             1047  AATT                
Tth111I       268  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              712  GACACTGTC           
Van91I        378  CCACCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1257  CCACCTGATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaII       BalI        BanI        BbvI        
BcnI        BmyI        BsaI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TfiI        TseI        Tsp45I      TspRI       
TthHB8I     XhoII       
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