Restriction map for pF1KA0968
 (Linear) (Six-base) MAPSORT of: KIAA0968.seq  Check: 8593  from: 1  to: 1434



KIAA0968.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      22    1434 
   Size:         22    1412
 
Acc65I G'GTAC_C

Cuts at:      0     626    1434 
   Size:        626     808
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     427     889     916    1434 
   Size:        220     207     462      27     518

  Fragments arranged by size: 

                 518     462     220     207      27
 
AflII C'TTAA_G

Cuts at:      0     796    1434 
   Size:        796     638
 
AleI CACnn'nnGTG

Cuts at:      0    1030    1434 
   Size:       1030     404
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1243    1434 
   Size:       1243     191
 
AlwNI CAG_nnn'CTG

Cuts at:      0     168     341     357    1311    1434 
   Size:        168     173      16     954     123

  Fragments arranged by size: 

                 954     173     168     123      16
 
ApaLI G'TGCA_C

Cuts at:      0     394    1228    1434 
   Size:        394     834     206

  Fragments arranged by size: 

                 834     394     206
 
AvaI C'yCGr_G

Cuts at:      0     308    1434 
   Size:        308    1126
 
BanI G'GyrC_C

Cuts at:      0     445     626     682    1364    1413    1434 
   Size:        445     181      56     682      49      21

  Fragments arranged by size: 

                 682     445     181      56      49      21
 
BbeI G_GCGC'C

Cuts at:      0     686    1417    1434 
   Size:        686     731      17

  Fragments arranged by size: 

                 731     686      17
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1028    1434 
   Size:       1028     406
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     355    1434 
   Size:        355    1079
 
BclI T'GATC_A

Cuts at:      0     740    1434 
   Size:        740     694
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1376    1434 
   Size:       1376      58
 
BglII A'GATC_T

Cuts at:      0    1405    1434 
   Size:       1405      29
 
Bme1580I G_kGCm'C

Cuts at:      0     398    1232    1434 
   Size:        398     834     202

  Fragments arranged by size: 

                 834     398     202
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1335    1434 
   Size:       1335      99
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     925    1434 
   Size:        925     509
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     379    1434 
   Size:        190     189    1055

  Fragments arranged by size: 

                1055     190     189
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424    1434 
   Size:        424    1010
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     149     847    1345    1434 
   Size:        149     698     498      89

  Fragments arranged by size: 

                 698     498     149      89
 
BsaHI Gr'CG_yC

Cuts at:      0     683    1414    1434 
   Size:        683     731      20

  Fragments arranged by size: 

                 731     683      20
 
BsaWI w'CCGG_w

Cuts at:      0     940    1434 
   Size:        940     494
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     256    1363    1434 
   Size:        256    1107      71

  Fragments arranged by size: 

                1107     256      71
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355    1434 
   Size:        355    1079
 
BsiHKAI G_wGCw'C

Cuts at:      0     398    1000    1232    1434 
   Size:        398     602     232     202

  Fragments arranged by size: 

                 602     398     232     202
 
BsiWI C'GTAC_G

Cuts at:      0     566    1434 
   Size:        566     868
 
Bsp1286I G_dGCh'C

Cuts at:      0     398    1000    1232    1434 
   Size:        398     602     232     202

  Fragments arranged by size: 

                 602     398     232     202
 
BspEI T'CCGG_A

Cuts at:      0     940    1434 
   Size:        940     494
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      22    1434 
   Size:         22    1412
 
BsrBI CCG'CTC

Cuts at:      0     821    1434 
   Size:        821     613
 
BsrDI GCAATG_nn'

Cuts at:      0    1092    1434 
   Size:       1092     342
 
BsrFI r'CCGG_y

Cuts at:      0     679    1434 
   Size:        679     755
 
BsrGI T'GTAC_A

Cuts at:      0     608    1434 
   Size:        608     826
 
BstBI TT'CG_AA

Cuts at:      0    1139    1434 
   Size:       1139     295
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     486     921    1408    1434 
   Size:        486     435     487      26

  Fragments arranged by size: 

                 487     486     435      26
 
BstYI r'GATC_y

Cuts at:      0     353     735     809    1405    1434 
   Size:        353     382      74     596      29

  Fragments arranged by size: 

                 596     382     353      74      29
 
Bsu36I CC'TnA_GG

Cuts at:      0    1145    1434 
   Size:       1145     289
 
BtgI C'CryG_G

Cuts at:      0     827     857    1434 
   Size:        827      30     577

  Fragments arranged by size: 

                 827     577      30
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     683     986    1269    1434 
   Size:        683     303     283     165

  Fragments arranged by size: 

                 683     303     283     165
 
BtsI GCAGTG_nn'

Cuts at:      0     372     458    1434 
   Size:        372      86     976

  Fragments arranged by size: 

                 976     372      86
 
ClaI AT'CG_AT

Cuts at:      0    1185    1434 
   Size:       1185     249
 
EaeI y'GGCC_r

Cuts at:      0       2     105     476     923    1434 
   Size:          2     103     371     447     511

  Fragments arranged by size: 

                 511     447     371     103       2
 
EarI CTCTTCn'nnn_

Cuts at:      0      26      49     943     961    1434 
   Size:         26      23     894      18     473

  Fragments arranged by size: 

                 894     473      26      23      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     343    1434 
   Size:        343    1091
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     220     379     427     889     916    1434 
   Size:        190      30     159      48     462      27     518

  Fragments arranged by size: 

                 518     462     190     159      48      30      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1165    1434 
   Size:       1165     269
 
EcoO109I rG'GnC_Cy

Cuts at:      0     402     561    1149    1170    1434 
   Size:        402     159     588      21     264

  Fragments arranged by size: 

                 588     402     264     159      21
 
EcoRV GAT'ATC

Cuts at:      0     300     535    1434 
   Size:        300     235     899

  Fragments arranged by size: 

                 899     300     235
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      84    1434 
   Size:         84    1350
 
HaeII r_GCGC'y

Cuts at:      0     686    1417    1434 
   Size:        686     731      17

  Fragments arranged by size: 

                 731     686      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     220     252     682     714    1084    1116    1434 
   Size:        220      32     430      32     370      32     318

  Fragments arranged by size: 

                 430     370     318     220      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     682    1413    1434 
   Size:        682     731      21

  Fragments arranged by size: 

                 731     682      21
 
KpnI G_GTAC'C

Cuts at:      0     630    1434 
   Size:        630     804
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     243    1325    1434 
   Size:        243    1082     109

  Fragments arranged by size: 

                1082     243     109
 
MscI TGG'CCA

Cuts at:      0       4     107     478     925    1434 
   Size:          4     103     371     447     509

  Fragments arranged by size: 

                 509     447     371     103       4
 
MslI CAynn'nnrTG

Cuts at:      0     484     916    1030    1111    1434 
   Size:        484     432     114      81     323

  Fragments arranged by size: 

                 484     432     323     114      81
 
MspA1I CmG'CkG

Cuts at:      0     784    1068    1434 
   Size:        784     284     366

  Fragments arranged by size: 

                 784     366     284
 
NaeI GCC'GGC

Cuts at:      0     681    1434 
   Size:        681     753
 
NarI GG'CG_CC

Cuts at:      0     683    1414    1434 
   Size:        683     731      20

  Fragments arranged by size: 

                 731     683      20
 
NgoMIV G'CCGG_C

Cuts at:      0     679    1434 
   Size:        679     755
 
NspI r_CATG'y

Cuts at:      0     843    1434 
   Size:        843     591
 
PasI CC'CwG_GG

Cuts at:      0    1152    1434 
   Size:       1152     282
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     384    1263    1434 
   Size:        384     879     171

  Fragments arranged by size: 

                 879     384     171
 
PfoI T'CCnGG_A

Cuts at:      0     356     528    1210    1434 
   Size:        356     172     682     224

  Fragments arranged by size: 

                 682     356     224     172
 
PpuMI rG'GwC_Cy

Cuts at:      0     402     561    1434 
   Size:        402     159     873

  Fragments arranged by size: 

                 873     402     159
 
PshAI GACnn'nnGTC

Cuts at:      0    1357    1434 
   Size:       1357      77
 
PsiI TTA'TAA

Cuts at:      0    1051    1434 
   Size:       1051     383
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     619     651    1434 
   Size:        619      32     783

  Fragments arranged by size: 

                 783     619      32
 
PvuII CAG'CTG

Cuts at:      0     784    1068    1434 
   Size:        784     284     366

  Fragments arranged by size: 

                 784     366     284
 
SapI GCTCTTCn'nnn_

Cuts at:      0      49     961    1434 
   Size:         49     912     473

  Fragments arranged by size: 

                 912     473      49
 
SexAI A'CCwGG_T

Cuts at:      0     269    1160    1434 
   Size:        269     891     274

  Fragments arranged by size: 

                 891     274     269
 
SfoI GGC'GCC

Cuts at:      0     684    1415    1434 
   Size:        684     731      19

  Fragments arranged by size: 

                 731     684      19
 
SmaI CCC'GGG

Cuts at:      0     310    1434 
   Size:        310    1124
 
SmlI C'TyrA_G

Cuts at:      0     439     796    1434 
   Size:        439     357     638

  Fragments arranged by size: 

                 638     439     357
 
SphI G_CATG'C

Cuts at:      0     843    1434 
   Size:        843     591
 
StyI C'CwwG_G

Cuts at:      0     731    1434 
   Size:        731     703
 
TatI w'GTAC_w

Cuts at:      0     608    1434 
   Size:        608     826
 
Tth111I GACn'n_nGTC

Cuts at:      0     271     715    1434 
   Size:        271     444     719

  Fragments arranged by size: 

                 719     444     271
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     704    1434 
   Size:        704     730
 
XmaI C'CCGG_G

Cuts at:      0     308    1434 
   Size:        308    1126
 
XmnI GAAnn'nnTTC

Cuts at:      0     874    1434 
   Size:        874     560
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AflII     AleI     AlfI    AlwNI    ApaLI
     AvaI     BanI     BbeI     BbsI    BciVI     BclI     BglI    BglII
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaHI    BsaWI
    BseRI     BsgI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BstBI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI     EaeI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI    HaeII    Hin4I     KasI     KpnI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI
    PflMI     PfoI    PpuMI    PshAI     PsiI     PsrI    PvuII     SapI
    SexAI     SfoI     SmaI     SmlI     SphI     StyI     TatI  Tth111I
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI   AflIII     AgeI     AhdI     AloI
     ApaI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BanII    BbvCI     BcgI    BfrBI     BlpI    BmgBI     BmtI   Bpu10I
    BsaAI    BsaBI    BsaXI    BseYI    BsiEI     BsmI    BsmBI    BspHI
   BssHII    BssSI   BstAPI   BstEII  BstZ17I    CspCI     DraI   DraIII
     DrdI     EagI  EcoICRI    EcoRI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     MfeI     MluI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI   PspOMI
    PspXI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI     SfcI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI    TaqII     TstI     XbaI     XhoI
     ZraI
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