Restriction map for pF1KA0943
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       BalI        BamHI       
BbrPI       BclI        BfrI        BglI        BlpI        
BpmI        Bpu1102I    BsaBI       BseAI       BsiWI       
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HpaI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MspA1I      MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       Tsp509I     Tth111I     XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII        1087  GACGTC               GACGT^C
AccII         930  CGCG                 CG^CG
ApaI          430  GGGCCC               GGGCC^C
Asp700I        49  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII         273  CCTAGG               C^CTAGG
BbeI          571  GGCGCC               GGCGC^C
BbvCI         628  GCTGAGG              CCTCAGC(-5/-2)
BcgI          920  GCATGAGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI          80  GGATAC               GTATCC(6/5)
BcnI          882  CCCGG                CC^[GC]GG
BglII         122  AGATCT               A^GATCT
BlnI          273  CCTAGG               C^CTAGG
BsaWI          32  TCCGGT               [AT]^CCGG[AT]
BseRI         814  GAGGAG               GAGGAG(10/8)
BsiEI         597  CGACCG               CG[GA][CT]^CG
BsmBI         721  GAGACG               CGTCTC(1/5)
BsmI          340  GCATTC               GAATGC(1/-1)
BspHI         740  TCATGA               T^CATGA
BstEII        633  GGTCACC              G^GT[ACGT]ACC
BstF5I        879  CATCC                GGATG(2/0)
BstPI         633  GGTCACC              G^GT[ACGT]ACC
BstUI         930  CGCG                 CG^CG
BstXI         947  CCATCGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         122  AGATCT               [GA]^GATC[CT]
EarI          303  GAAGAG               CTCTTC(1/4)
EcoO65I       633  GGTCACC              G^GT[ACGT]ACC
FauI          909  CCCGC                CCCGC(4/6)
FokI          879  CATCC                GGATG(9/13)
HindIII       460  AAGCTT               A^AGCTT
KasI          571  GGCGCC               G^GCGCC
Ksp632I       303  GAAGAG               CTCTTC(1/4)
MflI          122  AGATCT               [GA]^GATC[CT]
MvnI          930  CGCG                 CG^CG
NaeI          266  GCCGGC               GCC^GGC
NarI          571  GGCGCC               GG^CGCC
NciI          882  CCCGG                CC^[GC]GG
NcoI          652  CCATGG               C^CATGG
NgoMIV        266  GCCGGC               G^CCGGC
NspI          221  GCATGC               [GA]CATG^[CT]
PleI         1144  GACTC                GAGTC(4/5)
PpuMI         449  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          587  CTGCAG               CTGCA^G
RcaI          740  TCATGA               T^CATGA
SfcI          587  CTGCAG               C^T[GA][CT]AG
SfoI          571  GGCGCC               GGC^GCC
SphI          221  GCATGC               GCATG^C
StuI          710  AGGCCT               AGG^CCT
Tsp45I        634  GTCAC                ^GT[GC]AC
XbaI          142  TCTAGA               T^CTAGA
XcmI         1040  CCATGTTTGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         122  AGATCT               [GA]^GATC[CT]
XmnI           49  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          426  GTAC                 GT^AC
              667  GTAC                
AlwNI         448  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              751  CAGTCCCTG           
AspHI         483  GAGCTC               G[AT]GC[AT]^C
              817  GAGCTC              
BbsI         1156  GAAGAC               GAAGAC(2/6)
              469  GTCTTC              
BpuAI        1156  GAAGAC               GAAGAC(2/6)
              469  GTCTTC              
BsaAI         477  TACGTG               [CT]AC^GT[GA]
              715  TACGTG              
BsaI           61  GAGACC               GGTCTC(1/5)
              657  GAGACC              
BsgI          546  GTGCAG               GTGCAG(16/14)
              566  GTGCAG              
BsiHKAI       483  GAGCTC               G[AT]GC[AT]^C
              817  GAGCTC              
BsrDI         501  CATTGC               GCAATG(2/0)
              505  GCAATG              
BsrFI         266  GCCGGC               [GA]^CCGG[CT]
              599  ACCGGC              
Cfr10I        266  GCCGGC               [GA]^CCGG[CT]
              599  ACCGGC              
DpnII         123  GATC                 ^GATC
              243  GATC                
DraII         192  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              449  AGGTCCT             
DsaI          652  CCATGG               C^C[GA][CT]GG
              854  CCGTGG              
EcoO109I      192  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              449  AGGTCCT             
HaeII         571  GGCGCC               [GA]GCGC^[CT]
              784  AGCGCC              
MaeIII        362  GTTAC                ^GT[ACGT]AC
              634  GTCAC               
MboI          123  GATC                 ^GATC
              243  GATC                
NdeII         123  GATC                 ^GATC
              243  GATC                
RsaI          426  GTAC                 GT^AC
              667  GTAC                
SacI          483  GAGCTC               GAGCT^C
              817  GAGCTC              
Sau3AI        123  GATC                 ^GATC
              243  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AcyI        AluI        AvaII       BanI        
BanII       BbvI        BfaI        BmyI        BsaHI       
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstNI       Cac8I       
CfoI        Cfr13I      DdeI        EcoRII      EcoT14I     
Fnu4HI      HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MboII       MnlI        
MslI        MspI        MvaI        MwoI        NlaIII      
NlaIV       PflMI       Sau96I      ScrFI       SfaNI       
StyI        TaqI        TfiI        TseI        TspRI       
TthHB8I     Van91I      
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