Restriction map for pF1KA0943
 (Linear) (Six-base) MAPSORT of: KIAA0943.seq  Check: 7152  from: 1  to: 1179



KIAA0943.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AatII G_ACGT'C

Cuts at:      0    1091    1179 
   Size:       1091      88
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     309     475     748     995    1179 
   Size:        309     166     273     247     184

  Fragments arranged by size: 

                 309     273     247     184     166
 
AleI CACnn'nnGTG

Cuts at:      0     520    1179 
   Size:        520     659
 
AlwNI CAG_nnn'CTG

Cuts at:      0     453     756    1179 
   Size:        453     303     423

  Fragments arranged by size: 

                 453     423     303
 
ApaI G_GGCC'C

Cuts at:      0     434    1179 
   Size:        434     745
 
AvrII C'CTAG_G

Cuts at:      0     273    1179 
   Size:        273     906
 
BanI G'GyrC_C

Cuts at:      0     269     571     995    1030    1179 
   Size:        269     302     424      35     149

  Fragments arranged by size: 

                 424     302     269     149      35
 
BanII G_rGCy'C

Cuts at:      0      74     434     487     695     821     863    1179 
   Size:         74     360      53     208     126      42     316

  Fragments arranged by size: 

                 360     316     208     126      74      53      42
 
BbeI G_GCGC'C

Cuts at:      0     575    1179 
   Size:        575     604
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     462    1163    1179 
   Size:        462     701      16

  Fragments arranged by size: 

                 701     462      16
 
BbvCI CC'TCA_GC

Cuts at:      0     629    1179 
   Size:        629     550
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     909     943    1179 
   Size:        909      34     236

  Fragments arranged by size: 

                 909     236      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      74    1179 
   Size:         74    1105
 
BglII A'GATC_T

Cuts at:      0     122    1179 
   Size:        122    1057
 
Bme1580I G_kGCm'C

Cuts at:      0     434    1035    1179 
   Size:        434     601     144

  Fragments arranged by size: 

                 601     434     144
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      94     744    1179 
   Size:         94     650     435

  Fragments arranged by size: 

                 650     435      94
 
Bpu10I CC'TnA_GC

Cuts at:      0     629    1179 
   Size:        629     550
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      55     651    1179 
   Size:         55     596     528

  Fragments arranged by size: 

                 596     528      55
 
BsaAI yAC'GTr

Cuts at:      0     479     717    1179 
   Size:        479     238     462

  Fragments arranged by size: 

                 479     462     238
 
BsaHI Gr'CG_yC

Cuts at:      0     572     761    1088    1179 
   Size:        572     189     327      91

  Fragments arranged by size: 

                 572     327     189      91
 
BsaWI w'CCGG_w

Cuts at:      0      32    1179 
   Size:         32    1147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     617     647    1179 
   Size:        617      30     532

  Fragments arranged by size: 

                 617     532      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     829    1179 
   Size:        829     350
 
BseYI C'CCAG_C

Cuts at:      0     212    1179 
   Size:        212     967
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     567     587    1179 
   Size:        567      20     592

  Fragments arranged by size: 

                 592     567      20
 
BsiEI CG_ry'CG

Cuts at:      0     600    1179 
   Size:        600     579
 
BsiHKAI G_wGCw'C

Cuts at:      0     487     821    1179 
   Size:        487     334     358

  Fragments arranged by size: 

                 487     358     334
 
BsmI GAATG_Cn'

Cuts at:      0     340    1179 
   Size:        340     839
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     715    1179 
   Size:        715     464
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     434     487     695     821     863    1035    1179 
   Size:         74     360      53     208     126      42     172     144

  Fragments arranged by size: 

                 360     208     172     144     126      74      53      42
            
 
BspHI T'CATG_A

Cuts at:      0     740    1179 
   Size:        740     439
 
BsrDI GCAATG_nn'

Cuts at:      0     500     512    1179 
   Size:        500      12     667

  Fragments arranged by size: 

                 667     500      12
 
BsrFI r'CCGG_y

Cuts at:      0     266     599    1179 
   Size:        266     333     580

  Fragments arranged by size: 

                 580     333     266
 
BstEII G'GTnAC_C

Cuts at:      0     633    1179 
   Size:        633     546
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     954    1179 
   Size:        954     225
 
BstYI r'GATC_y

Cuts at:      0     122    1179 
   Size:        122    1057
 
BtgI C'CryG_G

Cuts at:      0     652     854    1179 
   Size:        652     202     325

  Fragments arranged by size: 

                 652     325     202
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     912     933    1179 
   Size:        912      21     246

  Fragments arranged by size: 

                 912     246      21
 
BtsI GCAGTG_nn'

Cuts at:      0     575     603     732     897    1179 
   Size:        575      28     129     165     282

  Fragments arranged by size: 

                 575     282     165     129      28
 
EarI CTCTTCn'nnn_

Cuts at:      0     298    1179 
   Size:        298     881
 
EcoICRI GAG'CTC

Cuts at:      0     485     819    1179 
   Size:        485     334     360

  Fragments arranged by size: 

                 485     360     334
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     309     475     748     995    1179 
   Size:        309     166     273     247     184

  Fragments arranged by size: 

                 309     273     247     184     166
 
EcoO109I rG'GnC_Cy

Cuts at:      0     193     450    1179 
   Size:        193     257     729

  Fragments arranged by size: 

                 729     257     193
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     753    1179 
   Size:        753     426
 
HaeII r_GCGC'y

Cuts at:      0     575     788    1179 
   Size:        575     213     391

  Fragments arranged by size: 

                 575     391     213
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     112     124     144     156     227     259     617     649 
   Size:        112      12      20      12      71      32     358      32

Cuts at:    649    1179 
   Size:        530

  Fragments arranged by size: 

                 530     358     112      71      32      32      20      12
                  12
 
HindIII A'AGCT_T

Cuts at:      0     460    1179 
   Size:        460     719
 
KasI G'GCGC_C

Cuts at:      0     571    1179 
   Size:        571     608
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     620    1179 
   Size:        620     559
 
MslI CAynn'nnrTG

Cuts at:      0     505     520     952    1179 
   Size:        505      15     432     227

  Fragments arranged by size: 

                 505     432     227      15
 
NaeI GCC'GGC

Cuts at:      0     268    1179 
   Size:        268     911
 
NarI GG'CG_CC

Cuts at:      0     572    1179 
   Size:        572     607
 
NcoI C'CATG_G

Cuts at:      0     652    1179 
   Size:        652     527
 
NgoMIV G'CCGG_C

Cuts at:      0     266    1179 
   Size:        266     913
 
NspI r_CATG'y

Cuts at:      0     225    1179 
   Size:        225     954
 
PasI CC'CwG_GG

Cuts at:      0     756    1179 
   Size:        756     423
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     181     258     756    1179 
   Size:        181      77     498     423

  Fragments arranged by size: 

                 498     423     181      77
 
PfoI T'CCnGG_A

Cuts at:      0     881    1179 
   Size:        881     298
 
PpuMI rG'GwC_Cy

Cuts at:      0     450    1179 
   Size:        450     729
 
PspOMI G'GGCC_C

Cuts at:      0     430    1179 
   Size:        430     749
 
PstI C_TGCA'G

Cuts at:      0     591    1179 
   Size:        591     588
 
SacI G_AGCT'C

Cuts at:      0     487     821    1179 
   Size:        487     334     358

  Fragments arranged by size: 

                 487     358     334
 
SfcI C'TryA_G

Cuts at:      0     587    1179 
   Size:        587     592
 
SfoI GGC'GCC

Cuts at:      0     573    1179 
   Size:        573     606
 
SphI G_CATG'C

Cuts at:      0     225    1179 
   Size:        225     954
 
StuI AGG'CCT

Cuts at:      0     712    1179 
   Size:        712     467
 
StyI C'CwwG_G

Cuts at:      0     273     488     652    1179 
   Size:        273     215     164     527

  Fragments arranged by size: 

                 527     273     215     164
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     567     599    1179 
   Size:        567      32     580

  Fragments arranged by size: 

                 580     567      32
 
XbaI T'CTAG_A

Cuts at:      0     142    1179 
   Size:        142    1037
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1047    1179 
   Size:       1047     132
 
XmnI GAAnn'nnTTC

Cuts at:      0      53    1179 
   Size:         53    1126
 
ZraI GAC'GTC

Cuts at:      0    1089    1179 
   Size:       1089      90
 

 Enzymes that do cut:

    AatII     AcuI     AleI    AlwNI     ApaI    AvrII     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BglII Bme1580I     BmrI
   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BsrDI
    BsrFI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     EarI
  EcoICRI  Eco57MI EcoO109I     FalI    HaeII    Hin4I  HindIII     KasI
     MmeI     MslI     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
    PflMI     PfoI    PpuMI   PspOMI     PstI     SacI     SfcI     SfoI
     SphI     StuI     StyI     TstI     XbaI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI     BaeI    BamHI     BclI    BfrBI     BglI     BlpI    BmgBI
     BmtI     BplI     BpmI    BpuEI    BsaBI    BsiWI    BspEI    BspMI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII     HpaI
     KpnI     MfeI     MluI     MscI   MspA1I     NdeI     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII
     TatI  Tth111I     XhoI     XmaI
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