Restriction map for pF1KA0929
 (Linear) (Six-base) MAPSORT of: KIAA0929.seq  Check: 5648  from: 1  to: 10869



KIAA0929.orf

 With 182 enzymes: SgfI * 

                        November 17, 2015 09:53  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3142    6619   10869 
   Size:       3142    3477    4250

  Fragments arranged by size: 

                4250    3477    3142
 
AatII G_ACGT'C

Cuts at:      0    2076   10869 
   Size:       2076    8793
 
AccI GT'mk_AC

Cuts at:      0    4976    7411   10869 
   Size:       4976    2435    3458

  Fragments arranged by size: 

                4976    3458    2435
 
AclI AA'CG_TT

Cuts at:      0   10715   10869 
   Size:      10715     154
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     959     980    2366    2611    3362    3896    5303 
   Size:        269     690      21    1386     245     751     534    1407

Cuts at:   5303    5674    8330    8779    9935   10402   10520   10869 
   Size:        371    2656     449    1156     467     118     349

  Fragments arranged by size: 

                2656    1407    1386    1156     751     690     534     467
                 449     371     349     269     245     118      21
 
AfeI AGC'GCT

Cuts at:      0    1255    2227    9224   10869 
   Size:       1255     972    6997    1645

  Fragments arranged by size: 

                6997    1645    1255     972
 
AflIII A'CryG_T

Cuts at:      0    2939    5902    6332    9480   10869 
   Size:       2939    2963     430    3148    1389

  Fragments arranged by size: 

                3148    2963    2939    1389     430
 
AgeI A'CCGG_T

Cuts at:      0    7191    9794   10869 
   Size:       7191    2603    1075

  Fragments arranged by size: 

                7191    2603    1075
 
AleI CACnn'nnGTG

Cuts at:      0    2560    5923    8005   10869 
   Size:       2560    3363    2082    2864

  Fragments arranged by size: 

                3363    2864    2560    2082
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    9082    9996   10045   10869 
   Size:       9082     914      49     824

  Fragments arranged by size: 

                9082     914     824      49
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     203     235    2253    2285    3639    3671   10869 
   Size:        203      32    2018      32    1354      32    7198

  Fragments arranged by size: 

                7198    2018    1354     203      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     648     823    4741    5134    5224    5281    5311    6642 
   Size:        648     175    3918     393      90      57      30    1331

Cuts at:   6642    7558    9391    9717   10410   10438   10869 
   Size:        916    1833     326     693      28     431

  Fragments arranged by size: 

                3918    1833    1331     916     693     648     431     393
                 326     175      90      57      30      28
 
ApaI G_GGCC'C

Cuts at:      0    5932    7943    8891    9276    9907   10511   10869 
   Size:       5932    2011     948     385     631     604     358

  Fragments arranged by size: 

                5932    2011     948     631     604     385     358
 
ApaLI G'TGCA_C

Cuts at:      0    6632    9763   10869 
   Size:       6632    3131    1106

  Fragments arranged by size: 

                6632    3131    1106
 
ApoI r'AATT_y

Cuts at:      0      13     949     958    1138    1168    3005    3799    3870 
   Size:         13     936       9     180      30    1837     794      71

Cuts at:   3870    4165    4293    7104    7773    8413   10306   10869 
   Size:        295     128    2811     669     640    1893     563

  Fragments arranged by size: 

                2811    1893    1837     936     794     669     640     563
                 295     180     128      71      30      13       9
 
AvaI C'yCGr_G

Cuts at:      0     395     551    1832    1907    2519    5089    5450    8611 
   Size:        395     156    1281      75     612    2570     361    3161

Cuts at:   8611    8883    8968    9236    9343    9377   10869 
   Size:        272      85     268     107      34    1492

  Fragments arranged by size: 

                3161    2570    1492    1281     612     395     361     272
                 268     156     107      85      75      34
 
AvrII C'CTAG_G

Cuts at:      0     484    3712   10869 
   Size:        484    3228    7157

  Fragments arranged by size: 

                7157    3228     484
 
BamHI G'GATC_C

Cuts at:      0    3696    7340   10869 
   Size:       3696    3644    3529

  Fragments arranged by size: 

                3696    3644    3529
 
BanI G'GyrC_C

Cuts at:      0    4008    4869    5576    6869    9531   10869 
   Size:       4008     861     707    1293    2662    1338

  Fragments arranged by size: 

                4008    2662    1338    1293     861     707
 
BanII G_rGCy'C

Cuts at:      0     422     492     869    4646    5645    5932    5956    7357 
   Size:        422      70     377    3777     999     287      24    1401

Cuts at:   7357    7407    7900    7943    8281    8618    8891    9243    9276 
   Size:         50     493      43     338     337     273     352      33

Cuts at:   9276    9907    9926   10511   10869 
   Size:        631      19     585     358

  Fragments arranged by size: 

                3777    1401     999     631     585     493     422     377
                 358     352     338     337     287     273      70      50
                  43      33      24      19
 
BbeI G_GCGC'C

Cuts at:      0    4873    5580    6873   10869 
   Size:       4873     707    1293    3996

  Fragments arranged by size: 

                4873    3996    1293     707
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2372    3263    3274    4118    4849    4897    6335    8350 
   Size:       2372     891      11     844     731      48    1438    2015

Cuts at:   8350   10869 
   Size:       2519

  Fragments arranged by size: 

                2519    2372    2015    1438     891     844     731      48
                  11
 
BbvCI CC'TCA_GC

Cuts at:      0    2965    5975    6422    6750    9554    9938   10869 
   Size:       2965    3010     447     328    2804     384     931

  Fragments arranged by size: 

                3010    2965    2804     931     447     384     328
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1469    1503    3345    3379   10869 
   Size:       1469      34    1842      34    7490

  Fragments arranged by size: 

                7490    1842    1469      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    9691   10869 
   Size:       9691    1178
 
BclI T'GATC_A

Cuts at:      0    3237   10869 
   Size:       3237    7632
 
BglI GCCn_nnn'nGGC

Cuts at:      0    6448    9547   10696   10869 
   Size:       6448    3099    1149     173

  Fragments arranged by size: 

                6448    3099    1149     173
 
BglII A'GATC_T

Cuts at:      0    3990    6112    6612   10757   10869 
   Size:       3990    2122     500    4145     112

  Fragments arranged by size: 

                4145    3990    2122     500     112
 
BlpI GC'TnA_GC

Cuts at:      0    5225   10869 
   Size:       5225    5644
 
Bme1580I G_kGCm'C

Cuts at:      0    5932    6636    7160    7943    8026    8891    9071    9276 
   Size:       5932     704     524     783      83     865     180     205

Cuts at:   9276    9767    9907   10143   10511   10869 
   Size:        491     140     236     368     358

  Fragments arranged by size: 

                5932     865     783     704     524     491     368     358
                 236     205     180     140      83
 
BmgBI CAC'GTC

Cuts at:      0    2940    5905    6333   10869 
   Size:       2940    2965     428    4536

  Fragments arranged by size: 

                4536    2965    2940     428
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     506     615     831    3148    3656    6276    6360    9058 
   Size:        506     109     216    2317     508    2620      84    2698

Cuts at:   9058    9066    9188    9692   10869 
   Size:          8     122     504    1177

  Fragments arranged by size: 

                2698    2620    2317    1177     508     506     504     216
                 122     109      84       8
 
BmtI G_CTAG'C

Cuts at:      0     656    1340   10869 
   Size:        656     684    9529

  Fragments arranged by size: 

                9529     684     656
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     696     776    1775    2108    2830    5288    6108    6409 
   Size:        696      80     999     333     722    2458     820     301

Cuts at:   6409    6617    6713    7406    7452    7625    7694    7988    9066 
   Size:        208      96     693      46     173      69     294    1078

Cuts at:   9066    9694    9753   10561   10869 
   Size:        628      59     808     308

  Fragments arranged by size: 

                2458    1078     999     820     808     722     696     693
                 628     333     308     301     294     208     173      96
                  80      69      59      46
 
Bpu10I CC'TnA_GC

Cuts at:      0    2774    2965    5975    6422    6750    7081    8280    9145 
   Size:       2774     191    3010     447     328     331    1199     865

Cuts at:   9145    9554    9899    9938   10115   10869 
   Size:        409     345      39     177     754

  Fragments arranged by size: 

                3010    2774    1199     865     754     447     409     345
                 331     328     191     177      39
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19    1378    2752    3093    3506    5416    5785    6947 
   Size:         19    1359    1374     341     413    1910     369    1162

Cuts at:   6947    7193    8474   10019   10405   10645   10869 
   Size:        246    1281    1545     386     240     224

  Fragments arranged by size: 

                1910    1545    1374    1359    1281    1162     413     386
                 369     341     246     240     224      19
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1582    2015    2637    3740    8776   10350   10869 
   Size:       1582     433     622    1103    5036    1574     519

  Fragments arranged by size: 

                5036    1582    1574    1103     622     519     433
 
BsaAI yAC'GTr

Cuts at:      0    4123   10749   10869 
   Size:       4123    6626     120

  Fragments arranged by size: 

                6626    4123     120
 
BsaBI GATnn'nnATC

Cuts at:      0    3695   10869 
   Size:       3695    7174
 
BsaHI Gr'CG_yC

Cuts at:      0    2073    4870    5210    5577    6870   10869 
   Size:       2073    2797     340     367    1293    3999

  Fragments arranged by size: 

                3999    2797    2073    1293     367     340
 
BsaWI w'CCGG_w

Cuts at:      0    2159    2271    7191    9794   10869 
   Size:       2159     112    4920    2603    1075

  Fragments arranged by size: 

                4920    2603    2159    1075     112
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     429     459    1253    1283    7404    7434    7450    7480 
   Size:        429      30     794      30    6121      30      16      30

Cuts at:   7480    7623    7653    8645    8675   10714   10744   10869 
   Size:        143      30     992      30    2039      30     125

  Fragments arranged by size: 

                6121    2039     992     794     429     143     125      30
                  30      30      30      30      30      30      16
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     629    2046    2148    3533    5438    5779    5782    5992 
   Size:        629    1417     102    1385    1905     341       3     210

Cuts at:   5992    5995    7046    7049    7216    7408    7796    8942    9224 
   Size:          3    1051       3     167     192     388    1146     282

Cuts at:   9224    9361    9899    9926   10869 
   Size:        137     538      27     943

  Fragments arranged by size: 

                1905    1417    1385    1146    1051     943     629     538
                 388     341     282     210     192     167     137     102
                  27       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1398    3115    5144    5274    6576    7181    7261    7936 
   Size:       1398    1717    2029     130    1302     605      80     675

Cuts at:   7936    8848    8890    8898    9001    9254    9306    9332    9830 
   Size:        912      42       8     103     253      52      26     498

Cuts at:   9830    9965    9992   10089   10270   10869 
   Size:        135      27      97     181     599

  Fragments arranged by size: 

                2029    1717    1398    1302     912     675     605     599
                 498     253     181     135     130     103      97      80
                  52      42      27      26       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     809    1000    3573    6479    8087    8108    9305    9415 
   Size:        809     191    2573    2906    1608      21    1197     110

Cuts at:   9415    9418    9775    9956    9967   10462   10869 
   Size:          3     357     181      11     495     407

  Fragments arranged by size: 

                2906    2573    1608    1197     809     495     407     357
                 191     181     110      21      11       3
 
BsiEI CG_ry'CG

Cuts at:      0    5824    8262   10869 
   Size:       5824    2438    2607

  Fragments arranged by size: 

                5824    2607    2438
 
BsiHKAI G_wGCw'C

Cuts at:      0    4646    5956    6636    7930    8324    9767   10869 
   Size:       4646    1310     680    1294     394    1443    1102

  Fragments arranged by size: 

                4646    1443    1310    1294    1102     680     394
 
BsmI GAATG_Cn'

Cuts at:      0    7227    7878    8037    8079   10869 
   Size:       7227     651     159      42    2790

  Fragments arranged by size: 

                7227    2790     651     159      42
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2099    2286    4917    7860   10461   10869 
   Size:       2099     187    2631    2943    2601     408

  Fragments arranged by size: 

                2943    2631    2601    2099     408     187
 
Bsp1286I G_dGCh'C

Cuts at:      0     422     492     869    4646    5645    5932    5956    6636 
   Size:        422      70     377    3777     999     287      24     680

Cuts at:   6636    7160    7357    7407    7900    7930    7943    8026    8281 
   Size:        524     197      50     493      30      13      83     255

Cuts at:   8281    8324    8618    8891    9071    9243    9276    9767    9907 
   Size:         43     294     273     180     172      33     491     140

Cuts at:   9907    9926   10143   10511   10869 
   Size:         19     217     368     358

  Fragments arranged by size: 

                3777     999     680     524     493     491     422     377
                 368     358     294     287     273     255     217     197
                 180     172     140      83      70      50      43      33
                  30      24      19      13
 
BspEI T'CCGG_A

Cuts at:      0    2271   10869 
   Size:       2271    8598
 
BspHI T'CATG_A

Cuts at:      0     465   10847   10869 
   Size:        465   10382      22

  Fragments arranged by size: 

               10382     465      22
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3142    3216    4950    4988    5796    6619   10107   10691 
   Size:       3142      74    1734      38     808     823    3488     584

Cuts at:  10691   10869 
   Size:        178

  Fragments arranged by size: 

                3488    3142    1734     823     808     584     178      74
                  38
 
BsrBI CCG'CTC

Cuts at:      0     402     579    5627   10869 
   Size:        402     177    5048    5242

  Fragments arranged by size: 

                5242    5048     402     177
 
BsrDI GCAATG_nn'

Cuts at:      0    7547    7715    8408   10856   10869 
   Size:       7547     168     693    2448      13

  Fragments arranged by size: 

                7547    2448     693     168      13
 
BsrFI r'CCGG_y

Cuts at:      0    5747    6075    6754    7191    8799    9794   10869 
   Size:       5747     328     679     437    1608     995    1075

  Fragments arranged by size: 

                5747    1608    1075     995     679     437     328
 
BssSI C'ACGA_G

Cuts at:      0    3502    4247   10869 
   Size:       3502     745    6622

  Fragments arranged by size: 

                6622    3502     745
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     823    4930    8074    8176    8506    9762   10410   10869 
   Size:        823    4107    3144     102     330    1256     648     459

  Fragments arranged by size: 

                4107    3144    1256     823     648     459     330     102
            
 
BstBI TT'CG_AA

Cuts at:      0    1453    1785    3972    4299   10869 
   Size:       1453     332    2187     327    6570

  Fragments arranged by size: 

                6570    2187    1453     332     327
 
BstEII G'GTnAC_C

Cuts at:      0     605    5050    5611    7251    9741   10869 
   Size:        605    4445     561    1640    2490    1128

  Fragments arranged by size: 

                4445    2490    1640    1128     605     561
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1258    5925    6381    8403   10869 
   Size:       1258    4667     456    2022    2466

  Fragments arranged by size: 

                4667    2466    2022    1258     456
 
BstYI r'GATC_y

Cuts at:      0     390     706    2805    3696    3990    4553    5124    6112 
   Size:        390     316    2099     891     294     563     571     988

Cuts at:   6112    6612    7340    8384   10757   10869 
   Size:        500     728    1044    2373     112

  Fragments arranged by size: 

                2373    2099    1044     988     891     728     571     563
                 500     390     316     294     112
 
Bsu36I CC'TnA_GG

Cuts at:      0    1805    5810    5909    7477   10869 
   Size:       1805    4005      99    1568    3392

  Fragments arranged by size: 

                4005    3392    1805    1568      99
 
BtgI C'CryG_G

Cuts at:      0    5606    5957    6398    7399    8015    9451    9512    9747 
   Size:       5606     351     441    1001     616    1436      61     235

Cuts at:   9747   10361   10869 
   Size:        614     508

  Fragments arranged by size: 

                5606    1436    1001     616     614     508     441     351
                 235      61
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    6393    8450   10869 
   Size:       6393    2057    2419

  Fragments arranged by size: 

                6393    2419    2057
 
BtsI GCAGTG_nn'

Cuts at:      0     505     756     765    1665    5213    5378    6140    6798 
   Size:        505     251       9     900    3548     165     762     658

Cuts at:   6798    7288    8075    8096    8177   10869 
   Size:        490     787      21      81    2692

  Fragments arranged by size: 

                3548    2692     900     787     762     658     505     490
                 251     165      81      21       9
 
ClaI AT'CG_AT

Cuts at:      0    3365   10869 
   Size:       3365    7504
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    7530    7565   10869 
   Size:       7530      35    3304

  Fragments arranged by size: 

                7530    3304      35
 
DraI TTT'AAA

Cuts at:      0    2855    3797   10869 
   Size:       2855     942    7072

  Fragments arranged by size: 

                7072    2855     942
 
DraIII CAC_nnn'GTG

Cuts at:      0    6810    9283   10869 
   Size:       6810    2473    1586

  Fragments arranged by size: 

                6810    2473    1586
 
DrdI GACnn_nn'nnGTC

Cuts at:      0   10207   10869 
   Size:      10207     662
 
EaeI y'GGCC_r

Cuts at:      0    3845    6527    8138    8393    8516    8801    9214   10008 
   Size:       3845    2682    1611     255     123     285     413     794

Cuts at:  10008   10620   10869 
   Size:        612     249

  Fragments arranged by size: 

                3845    2682    1611     794     612     413     285     255
                 249     123
 
EarI CTCTTCn'nnn_

Cuts at:      0     995    1719    3782    4245    4424    4880    5398    5756 
   Size:        995     724    2063     463     179     456     518     358

Cuts at:   5756    7433   10220   10869 
   Size:       1677    2787     649

  Fragments arranged by size: 

                2787    2063    1677     995     724     649     518     463
                 456     358     179
 
EcoICRI GAG'CTC

Cuts at:      0    4644    5954   10869 
   Size:       4644    1310    4915

  Fragments arranged by size: 

                4915    4644    1310
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     696     776     959     980    1775    2108    2366 
   Size:        269     427      80     183      21     795     333     258

Cuts at:   2366    2611    2830    3362    3896    5288    5303    5674    6108 
   Size:        245     219     532     534    1392      15     371     434

Cuts at:   6108    6409    6617    6713    7406    7452    7625    7694    7988 
   Size:        301     208      96     693      46     173      69     294

Cuts at:   7988    8330    8779    9066    9694    9753    9935   10402   10520 
   Size:        342     449     287     628      59     182     467     118

Cuts at:  10520   10561   10869 
   Size:         41     308

  Fragments arranged by size: 

                1392     795     693     628     534     532     467     449
                 434     427     371     342     333     308     301     294
                 287     269     258     245     219     208     183     182
                 173     118      96      80      69      59      46      41
                  21      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    7582    9157    9914   10869 
   Size:       7582    1575     757     955

  Fragments arranged by size: 

                7582    1575     955     757
 
EcoO109I rG'GnC_Cy

Cuts at:      0     677    5093    5831    5929    6465    7587    7885    7960 
   Size:        677    4416     738      98     536    1122     298      75

Cuts at:   7960    8485    8790    8887    9272    9273    9549    9903    9904 
   Size:        525     305      97     385       1     276     354       1

Cuts at:   9904   10058   10407   10507   10508   10869 
   Size:        154     349     100       1     361

  Fragments arranged by size: 

                4416    1122     738     677     536     525     385     361
                 354     349     305     298     276     154     100      98
                  97      75       1       1       1
 
EcoRI G'AATT_C

Cuts at:      0    3870    7104   10306   10869 
   Size:       3870    3234    3202     563

  Fragments arranged by size: 

                3870    3234    3202     563
 
EcoRV GAT'ATC

Cuts at:      0     531    3585   10869 
   Size:        531    3054    7284

  Fragments arranged by size: 

                7284    3054     531
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     955    1090    2599    2764    3025    4234    5528    6865 
   Size:        955     135    1509     165     261    1209    1294    1337

Cuts at:   6865    7541    7806   10686   10869 
   Size:        676     265    2880     183

  Fragments arranged by size: 

                2880    1509    1337    1294    1209     955     676     265
                 261     183     165     135
 
HaeII r_GCGC'y

Cuts at:      0    1257    2229    2838    4873    5580    6643    6873    8372 
   Size:       1257     972     609    2035     707    1063     230    1499

Cuts at:   8372    9025    9226   10869 
   Size:        653     201    1643

  Fragments arranged by size: 

                2035    1643    1499    1257    1063     972     707     653
                 609     230     201
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     241     273     429     461     996    1028    2929    2961 
   Size:        241      32     156      32     535      32    1901      32

Cuts at:   2961    3479    3511    4264    4296    6199    6231    6259    6291 
   Size:        518      32     753      32    1903      32      28      32

Cuts at:   6291    6667    6699    7093    7125    7450    7482    8244    8263 
   Size:        376      32     394      32     325      32     762      19

Cuts at:   8263    8276    8295    8581    8613    9882    9914   10869 
   Size:         13      19     286      32    1269      32     955

  Fragments arranged by size: 

                1903    1901    1269     955     762     753     535     518
                 394     376     325     286     241     156      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      28      19      19      13
 
HincII GTy'rAC

Cuts at:      0    4326    7458    7605    8186    8871   10869 
   Size:       4326    3132     147     581     685    1998

  Fragments arranged by size: 

                4326    3132    1998     685     581     147
 
HindIII A'AGCT_T

Cuts at:      0    2581    4211    5467    8752   10869 
   Size:       2581    1630    1256    3285    2117

  Fragments arranged by size: 

                3285    2581    2117    1630    1256
 
KasI G'GCGC_C

Cuts at:      0    4869    5576    6869   10869 
   Size:       4869     707    1293    4000

  Fragments arranged by size: 

                4869    4000    1293     707
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3693    5107    6977    7165   10853   10869 
   Size:       3693    1414    1870     188    3688      16

  Fragments arranged by size: 

                3693    3688    1870    1414     188      16
 
MscI TGG'CCA

Cuts at:      0    3847    8518   10869 
   Size:       3847    4671    2351

  Fragments arranged by size: 

                4671    3847    2351
 
MslI CAynn'nnrTG

Cuts at:      0    2560    3689    4651    5923    8005    8248    9043    9064 
   Size:       2560    1129     962    1272    2082     243     795      21

Cuts at:   9064    9479   10869 
   Size:        415    1390

  Fragments arranged by size: 

                2560    2082    1390    1272    1129     962     795     415
                 243      21
 
MspA1I CmG'CkG

Cuts at:      0     692    1579    1653    3222    4965    5368    5704    5848 
   Size:        692     887      74    1569    1743     403     336     144

Cuts at:   5848    8059    8104    8361    8715    8929    9246    9310    9514 
   Size:       2211      45     257     354     214     317      64     204

Cuts at:   9514    9773    9895   10093   10435   10554   10869 
   Size:        259     122     198     342     119     315

  Fragments arranged by size: 

                2211    1743    1569     887     692     403     354     342
                 336     317     315     259     257     214     204     198
                 144     122     119      74      64      45
 
NaeI GCC'GGC

Cuts at:      0    5749    6756    8801   10869 
   Size:       5749    1007    2045    2068

  Fragments arranged by size: 

                5749    2068    2045    1007
 
NarI GG'CG_CC

Cuts at:      0    4870    5577    6870   10869 
   Size:       4870     707    1293    3999

  Fragments arranged by size: 

                4870    3999    1293     707
 
NcoI C'CATG_G

Cuts at:      0    5606    5957    9747   10869 
   Size:       5606     351    3790    1122

  Fragments arranged by size: 

                5606    3790    1122     351
 
NgoMIV G'CCGG_C

Cuts at:      0    5747    6754    8799   10869 
   Size:       5747    1007    2045    2070

  Fragments arranged by size: 

                5747    2070    2045    1007
 
NheI G'CTAG_C

Cuts at:      0     652    1336   10869 
   Size:        652     684    9533

  Fragments arranged by size: 

                9533     684     652
 
NruI TCG'CGA

Cuts at:      0     494   10869 
   Size:        494   10375
 
NspI r_CATG'y

Cuts at:      0    1089    1419    9484   10869 
   Size:       1089     330    8065    1385

  Fragments arranged by size: 

                8065    1385    1089     330
 
PasI CC'CwG_GG

Cuts at:      0    6182    8236    8649    8676    9159    9506   10869 
   Size:       6182    2054     413      27     483     347    1363

  Fragments arranged by size: 

                6182    2054    1363     483     413     347      27
 
PciI A'CATG_T

Cuts at:      0    9480   10869 
   Size:       9480    1389
 
PflMI CCAn_nnn'nTGG

Cuts at:      0   10400   10869 
   Size:      10400     469
 
PfoI T'CCnGG_A

Cuts at:      0    2085    6594    6692    9880   10869 
   Size:       2085    4509      98    3188     989

  Fragments arranged by size: 

                4509    3188    2085     989      98
 
PpuMI rG'GwC_Cy

Cuts at:      0     677    5831    8790   10058   10869 
   Size:        677    5154    2959    1268     811

  Fragments arranged by size: 

                5154    2959    1268     811     677
 
PshAI GACnn'nnGTC

Cuts at:      0    8261    8788    9490   10869 
   Size:       8261     527     702    1379

  Fragments arranged by size: 

                8261    1379     702     527
 
PspOMI G'GGCC_C

Cuts at:      0    5928    7939    8887    9272    9903   10507   10869 
   Size:       5928    2011     948     385     631     604     362

  Fragments arranged by size: 

                5928    2011     948     631     604     385     362
 
PspXI vC'TCGA_Gb

Cuts at:      0    1832    2519    9377   10869 
   Size:       1832     687    6858    1492

  Fragments arranged by size: 

                6858    1832    1492     687
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1283    1315    4153    4185   10869 
   Size:       1283      32    2838      32    6684

  Fragments arranged by size: 

                6684    2838    1283      32      32
 
PstI C_TGCA'G

Cuts at:      0     816    2787    3227    5220    5373    5532    5703    5793 
   Size:        816    1971     440    1993     153     159     171      90

Cuts at:   5793    5847    6195    6647    7308    8502    9057    9251    9932 
   Size:         54     348     452     661    1194     555     194     681

Cuts at:   9932   10688   10757   10869 
   Size:        756      69     112

  Fragments arranged by size: 

                1993    1971    1194     816     756     681     661     555
                 452     440     348     194     171     159     153     112
                  90      69      54
 
PvuI CG_AT'CG

Cuts at:      0    8262   10869 
   Size:       8262    2607
 
PvuII CAG'CTG

Cuts at:      0    1579    1653    3222    4965    5368    5848    8715    8929 
   Size:       1579      74    1569    1743     403     480    2867     214

Cuts at:   8929    9310    9895   10093   10435   10554   10869 
   Size:        381     585     198     342     119     315

  Fragments arranged by size: 

                2867    1743    1579    1569     585     480     403     381
                 342     315     214     198     119      74
 
SacI G_AGCT'C

Cuts at:      0    4646    5956   10869 
   Size:       4646    1310    4913

  Fragments arranged by size: 

                4913    4646    1310
 
SacII CC_GC'GG

Cuts at:      0    9515   10869 
   Size:       9515    1354
 
SanDI GG'GwC_CC

Cuts at:      0   10058   10869 
   Size:      10058     811
 
SapI GCTCTTCn'nnn_

Cuts at:      0    4424    7433   10869 
   Size:       4424    3009    3436

  Fragments arranged by size: 

                4424    3436    3009
 
SbfI CC_TGCA'GG

Cuts at:      0    8502   10688   10869 
   Size:       8502    2186     181

  Fragments arranged by size: 

                8502    2186     181
 
ScaI AGT'ACT

Cuts at:      0    9009   10869 
   Size:       9009    1860
 
SexAI A'CCwGG_T

Cuts at:      0    3999   10869 
   Size:       3999    6870
 
SfcI C'TryA_G

Cuts at:      0     384     534     812     914    2172    2783    3223    5216 
   Size:        384     150     278     102    1258     611     440    1993

Cuts at:   5216    5369    5528    5699    5789    5843    6191    6643    6710 
   Size:        153     159     171      90      54     348     452      67

Cuts at:   6710    6929    7304    8498    8861    9053    9204    9247    9928 
   Size:        219     375    1194     363     192     151      43     681

Cuts at:   9928   10684   10753   10869 
   Size:        756      69     116

  Fragments arranged by size: 

                1993    1258    1194     756     681     611     452     440
                 384     375     363     348     278     219     192     171
                 159     153     151     150     116     102      90      69
                  67      54      43
 
SfoI GGC'GCC

Cuts at:      0    4871    5578    6871   10869 
   Size:       4871     707    1293    3998

  Fragments arranged by size: 

                4871    3998    1293     707
 
SgrAI Cr'CCGG_yG

Cuts at:      0    5747    7191   10869 
   Size:       5747    1444    3678

  Fragments arranged by size: 

                5747    3678    1444
 
SmaI CCC'GGG

Cuts at:      0     553    5091    8970   10869 
   Size:        553    4538    3879    1899

  Fragments arranged by size: 

                4538    3879    1899     553
 
SmlI C'TyrA_G

Cuts at:      0      34     395    1393    1832    2519    2767    3108    3521 
   Size:         34     361     998     439     687     248     341     413

Cuts at:   3521    5431    5800    6962    7208    8489    9377   10034   10420 
   Size:       1910     369    1162     246    1281     888     657     386

Cuts at:  10420   10660   10869 
   Size:        240     209

  Fragments arranged by size: 

                1910    1281    1162     998     888     687     657     439
                 413     386     369     361     341     248     246     240
                 209      34
 
SpeI A'CTAG_T

Cuts at:      0    9408   10869 
   Size:       9408    1461
 
SphI G_CATG'C

Cuts at:      0    1089    1419   10869 
   Size:       1089     330    9450

  Fragments arranged by size: 

                9450    1089     330
 
StuI AGG'CCT

Cuts at:      0    5733    6427    6726    7447   10869 
   Size:       5733     694     299     721    3422

  Fragments arranged by size: 

                5733    3422     721     694     299
 
StyI C'CwwG_G

Cuts at:      0     484    1148    1869    3712    5606    5780    5827    5862 
   Size:        484     664     721    1843    1894     174      47      35

Cuts at:   5862    5957    5966    6770    7790    8600    8786    8986    9747 
   Size:         95       9     804    1020     810     186     200     761

Cuts at:   9747   10869 
   Size:       1122

  Fragments arranged by size: 

                1894    1843    1122    1020     810     804     761     721
                 664     484     200     186     174      95      47      35
                   9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     593     843    4068    5599    6379    7275    8249    8362 
   Size:        593     250    3225    1531     780     896     974     113

Cuts at:   8362    8536    8662    8689    9107    9145    9988   10141   10472 
   Size:        174     126      27     418      38     843     153     331

Cuts at:  10472   10666   10869 
   Size:        194     203

  Fragments arranged by size: 

                3225    1531     974     896     843     780     593     418
                 331     250     203     194     174     153     126     113
                  38      27
 
TatI w'GTAC_w

Cuts at:      0    6999    7069    9007   10869 
   Size:       6999      70    1938    1862

  Fragments arranged by size: 

                6999    1938    1862      70
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1253    1285    1593    1625    8224    8256    8645    8677 
   Size:       1253      32     308      32    6599      32     389      32

Cuts at:   8677    9834    9866   10333   10365   10869 
   Size:       1157      32     467      32     504

  Fragments arranged by size: 

                6599    1253    1157     504     467     389     308      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2596    8764    9138   10653   10869 
   Size:       2596    6168     374    1515     216

  Fragments arranged by size: 

                6168    2596    1515     374     216
 
XbaI T'CTAG_A

Cuts at:      0     625     664     697    2947    4512    4815   10869 
   Size:        625      39      33    2250    1565     303    6054

  Fragments arranged by size: 

                6054    2250    1565     625     303      39      33
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     174    5673    5813    5922    6132    6265    9045   10456 
   Size:        174    5499     140     109     210     133    2780    1411

Cuts at:  10456   10869 
   Size:        413

  Fragments arranged by size: 

                5499    2780    1411     413     210     174     140     133
                 109
 
XhoI C'TCGA_G

Cuts at:      0     395    1832    2519    9377   10869 
   Size:        395    1437     687    6858    1492

  Fragments arranged by size: 

                6858    1492    1437     687     395
 
XmaI C'CCGG_G

Cuts at:      0     551    5089    8968   10869 
   Size:        551    4538    3879    1901

  Fragments arranged by size: 

                4538    3879    1901     551
 
XmnI GAAnn'nnTTC

Cuts at:      0    9224   10869 
   Size:       9224    1645
 
ZraI GAC'GTC

Cuts at:      0    2074   10869 
   Size:       2074    8795
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI     AfeI   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI
    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
    HaeII    Hin4I   HincII  HindIII     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NruI     NspI
     PasI     PciI    PflMI     PfoI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII     SacI    SacII    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfoI    SgrAI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I    AflII     AhdI     AscI     AseI    AsiSI     BaeI    BfrBI
     BplI    BsiWI    BsrGI   BssHII  BstZ17I     EagI     EciI     FseI
     FspI    FspAI     HpaI     KpnI     MfeI     MluI     NdeI     NotI
     NsiI     PacI     PmeI     PmlI     PpiI     PsiI    RsrII     SalI
     SfiI     SgfI    SnaBI     SrfI     SspI     SwaI
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