Restriction map for pF1KA0925
 (Linear) (Six-base) MAPSORT of: KIAA0925.seq  Check: 5705  from: 1  to: 1425



KIAA0925.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AccI GT'mk_AC

Cuts at:      0      44    1425 
   Size:         44    1381
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370    1425 
   Size:        370    1055
 
AfeI AGC'GCT

Cuts at:      0     450    1425 
   Size:        450     975
 
AflIII A'CryG_T

Cuts at:      0     311    1425 
   Size:        311    1114
 
AleI CACnn'nnGTG

Cuts at:      0     119     316     526    1425 
   Size:        119     197     210     899

  Fragments arranged by size: 

                 899     210     197     119
 
ApaLI G'TGCA_C

Cuts at:      0     103    1425 
   Size:        103    1322
 
AvaI C'yCGr_G

Cuts at:      0     337    1425 
   Size:        337    1088
 
AvrII C'CTAG_G

Cuts at:      0     948    1425 
   Size:        948     477
 
BamHI G'GATC_C

Cuts at:      0      83    1425 
   Size:         83    1342
 
BanI G'GyrC_C

Cuts at:      0     419    1425 
   Size:        419    1006
 
BanII G_rGCy'C

Cuts at:      0     611     794    1040    1298    1425 
   Size:        611     183     246     258     127

  Fragments arranged by size: 

                 611     258     246     183     127
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1088    1425 
   Size:       1088     337
 
BglI GCCn_nnn'nGGC

Cuts at:      0     245    1425 
   Size:        245    1180
 
Bme1580I G_kGCm'C

Cuts at:      0     107     392    1058    1425 
   Size:        107     285     666     367

  Fragments arranged by size: 

                 666     367     285     107
 
BmgBI CAC'GTC

Cuts at:      0     314    1425 
   Size:        314    1111
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     630     774     942    1059    1425 
   Size:        630     144     168     117     366

  Fragments arranged by size: 

                 630     366     168     144     117
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     397     696    1366    1425 
   Size:        214     183     299     670      59

  Fragments arranged by size: 

                 670     299     214     183      59
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1006    1425 
   Size:       1006     419
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     714    1425 
   Size:        714     711
 
BsaAI yAC'GTr

Cuts at:      0     578    1425 
   Size:        578     847
 
BsaBI GATnn'nnATC

Cuts at:      0      88    1425 
   Size:         88    1337
 
BsaHI Gr'CG_yC

Cuts at:      0       9    1425 
   Size:          9    1416
 
BsaWI w'CCGG_w

Cuts at:      0      35     869    1425 
   Size:         35     834     556

  Fragments arranged by size: 

                 834     556      35
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      10      40     298     328    1425 
   Size:         10      30     258      30    1097

  Fragments arranged by size: 

                1097     258      30      30      10
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     685    1360    1425 
   Size:        685     675      65

  Fragments arranged by size: 

                 685     675      65
 
BseYI C'CCAG_C

Cuts at:      0     930    1137    1425 
   Size:        930     207     288

  Fragments arranged by size: 

                 930     288     207
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367    1425 
   Size:        367    1058
 
BsiHKAI G_wGCw'C

Cuts at:      0      71     107     304    1298    1425 
   Size:         71      36     197     994     127

  Fragments arranged by size: 

                 994     197     127      71      36
 
BsmI GAATG_Cn'

Cuts at:      0    1370    1425 
   Size:       1370      55
 
Bsp1286I G_dGCh'C

Cuts at:      0      71     107     304     392     611     794    1040    1058 
   Size:         71      36     197      88     219     183     246      18

Cuts at:   1058    1298    1425 
   Size:        240     127

  Fragments arranged by size: 

                 246     240     219     197     183     127      88      71
                  36      18
 
BspEI T'CCGG_A

Cuts at:      0      35    1425 
   Size:         35    1390
 
BsrBI CCG'CTC

Cuts at:      0     614     926    1425 
   Size:        614     312     499

  Fragments arranged by size: 

                 614     499     312
 
BsrDI GCAATG_nn'

Cuts at:      0     255     290    1425 
   Size:        255      35    1135

  Fragments arranged by size: 

                1135     255      35
 
BsrFI r'CCGG_y

Cuts at:      0     395     498    1425 
   Size:        395     103     927

  Fragments arranged by size: 

                 927     395     103
 
BssSI C'ACGA_G

Cuts at:      0     297    1425 
   Size:        297    1128
 
BstBI TT'CG_AA

Cuts at:      0    1302    1425 
   Size:       1302     123
 
BstYI r'GATC_y

Cuts at:      0      83     323     332    1155    1425 
   Size:         83     240       9     823     270

  Fragments arranged by size: 

                 823     270     240      83       9
 
BtsI GCAGTG_nn'

Cuts at:      0      69    1425 
   Size:         69    1356
 
DraIII CAC_nnn'GTG

Cuts at:      0     578     657    1425 
   Size:        578      79     768

  Fragments arranged by size: 

                 768     578      79
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     466    1425 
   Size:        466     959
 
EaeI y'GGCC_r

Cuts at:      0      56     143     237     618    1425 
   Size:         56      87      94     381     807

  Fragments arranged by size: 

                 807     381      94      87      56
 
EarI CTCTTCn'nnn_

Cuts at:      0     770    1228    1425 
   Size:        770     458     197

  Fragments arranged by size: 

                 770     458     197
 
EcoICRI GAG'CTC

Cuts at:      0    1296    1425 
   Size:       1296     129
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     370     397     696    1366    1425 
   Size:        214     156      27     299     670      59

  Fragments arranged by size: 

                 670     299     214     156      59      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0     468     753    1026    1425 
   Size:        468     285     273     399

  Fragments arranged by size: 

                 468     399     285     273
 
EcoRV GAT'ATC

Cuts at:      0     261    1425 
   Size:        261    1164
 
FspI TGC'GCA

Cuts at:      0    1405    1425 
   Size:       1405      20
 
HaeII r_GCGC'y

Cuts at:      0     373     452    1425 
   Size:        373      79     973

  Fragments arranged by size: 

                 973     373      79
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     298     330     778     810    1425 
   Size:        298      32     448      32     615

  Fragments arranged by size: 

                 615     448     298      32      32
 
HincII GTy'rAC

Cuts at:      0     129    1248    1425 
   Size:        129    1119     177

  Fragments arranged by size: 

                1119     177     129
 
MscI TGG'CCA

Cuts at:      0      58     145    1425 
   Size:         58      87    1280

  Fragments arranged by size: 

                1280      87      58
 
MslI CAynn'nnrTG

Cuts at:      0     119     316     524     526     832    1045    1051    1425 
   Size:        119     197     208       2     306     213       6     374

  Fragments arranged by size: 

                 374     306     213     208     197     119       6       2
            
 
MspA1I CmG'CkG

Cuts at:      0    1386    1425 
   Size:       1386      39
 
NaeI GCC'GGC

Cuts at:      0     397    1425 
   Size:        397    1028
 
NgoMIV G'CCGG_C

Cuts at:      0     395    1425 
   Size:        395    1030
 
NspI r_CATG'y

Cuts at:      0     543     837    1425 
   Size:        543     294     588

  Fragments arranged by size: 

                 588     543     294
 
PfoI T'CCnGG_A

Cuts at:      0     374    1425 
   Size:        374    1051
 
PmlI CAC'GTG

Cuts at:      0     578    1425 
   Size:        578     847
 
PpuMI rG'GwC_Cy

Cuts at:      0     468     753    1425 
   Size:        468     285     672

  Fragments arranged by size: 

                 672     468     285
 
PshAI GACnn'nnGTC

Cuts at:      0     706    1425 
   Size:        706     719
 
PstI C_TGCA'G

Cuts at:      0     632    1425 
   Size:        632     793
 
PvuII CAG'CTG

Cuts at:      0    1386    1425 
   Size:       1386      39
 
SacI G_AGCT'C

Cuts at:      0    1298    1425 
   Size:       1298     127
 
SfcI C'TryA_G

Cuts at:      0     628    1425 
   Size:        628     797
 
SmlI C'TyrA_G

Cuts at:      0    1021    1425 
   Size:       1021     404
 
StuI AGG'CCT

Cuts at:      0     947    1425 
   Size:        947     478
 
StyI C'CwwG_G

Cuts at:      0     227     948    1425 
   Size:        227     721     477

  Fragments arranged by size: 

                 721     477     227
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     481     844    1293    1425 
   Size:        481     363     449     132

  Fragments arranged by size: 

                 481     449     363     132
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     969    1425 
   Size:        969     456
 
XmnI GAAnn'nnTTC

Cuts at:      0     274    1425 
   Size:        274    1151
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AleI    ApaLI     AvaI    AvrII
    BamHI     BanI    BanII     BbsI     BglI Bme1580I    BmgBI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BsrBI
    BsrDI    BsrFI    BssSI    BstBI    BstYI     BtsI   DraIII     DrdI
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRV     FspI    HaeII
    Hin4I   HincII     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI
     PfoI     PmlI    PpuMI    PshAI     PstI    PvuII     SacI     SfcI
     SmlI     StuI     StyI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AlfI
     AloI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI     BaeI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
     BmrI     BmtI   Bpu10I    BsiEI    BsiWI    BsmBI    BspHI    BspMI
    BsrGI   BssHII   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI     EagI     EciI    EcoNI    EcoRI
     FalI     FseI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PpiI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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