Restriction map for pF1KA0816
##################################
# NO CUT ENZYMES (   45 enzymes) #
##################################

AatII       AclI        AflII       AscI        AseI        
AsnI        BcgI        BfrI        BglII       BsiWI       
BspDI       BspHI       BstBI       ClaI        DraI        
EagI        EclXI       Eco52I      EcoNI       EcoRI       
FseI        HindIII     KspI        MluI        NheI        
NotI        NruI        NspV        PacI        PmeI        
PshBI       Psp1406I    PvuI        RcaI        SacII       
SalI        SexAI       SfiI        SfuI        SnaBI       
SpeI        SphI        SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AgeI         3750  ACCGGT               A^CCGGT
AhdI         5527  GACACGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       5111  GTGCAC               G^TGCAC
AocI         2672  CCTCAGG              CC^T[ACGT]AGG
ApaI         1625  GGGCCC               GGGCC^C
ApaLI        5111  GTGCAC               G^TGCAC
AspEI        5527  GACACGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         4716  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1472  GGATCC               G^GATCC
BciVI        2433  GGATAC               GTATCC(6/5)
BclI         4394  TGATCA               T^GATCA
BsiEI        1284  CGACCG               CG[GA][CT]^CG
BssHII         17  GCGCGC               G^CGCGC
BssSI        3862  CTCGTG               CACGAG(-5/-1)
BstEII       2475  GGTCACC              G^GT[ACGT]ACC
BstPI        2475  GGTCACC              G^GT[ACGT]ACC
Bsu36I       2672  CCTCAGG              CC^T[ACGT]AGG
CpoI         3580  CGGTCCG              CG^G[AT]CCG
DraIII       3011  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     5527  GACACGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2672  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      2475  GGTCACC              G^GT[ACGT]ACC
EcoRV        4795  GATATC               GAT^ATC
EcoT22I      5514  ATGCAT               ATGCA^T
FbaI         4394  TGATCA               T^GATCA
HpaI         1078  GTTAAC               GTT^AAC
MfeI         2777  CAATTG               C^AATTG
MunI         2777  CAATTG               C^AATTG
NdeI         3764  CATATG               CA^TATG
NsiI         5514  ATGCAT               ATGCA^T
PflFI        4716  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3750  ACCGGT               A^CCGGT
RsrII        3580  CGGTCCG              CG^G[AT]CCG
SacI         4147  GAGCTC               GAGCT^C
SbfI         2086  CCTGCAGG             CCTGCA^GG
SgrAI        3749  CACCGGTG             C[GA]^CCGG[CT]G
Sse8387I     2086  CCTGCAGG             CCTGCA^GG
Tth111I      4716  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccIII        368  TCCGGA               T^CCGGA
             3583  TCCGGA              
AcsI         2002  AAATTT               [GA]^AATT[CT]
             2782  GAATTT              
Aor51HI      1993  AGCGCT               AGC^GCT
             2908  AGCGCT              
ApoI         2002  AAATTT               [GA]^AATT[CT]
             2782  GAATTT              
AvrII        3627  CCTAGG               C^CTAGG
             4966  CCTAGG              
BbeI           31  GGCGCC               GGCGC^C
             1826  GGCGCC              
BbrPI        1173  CACGTG               CAC^GTG
             2356  CACGTG              
BbvCI         548  CCTCAGC              CCTCAGC(-5/-2)
             4813  CCTCAGC             
BlnI         3627  CCTAGG               C^CTAGG
             4966  CCTAGG              
BlpI          484  GCTGAGC              GC^T[ACGT]AGC
             1849  GCTAAGC             
Bpu1102I      484  GCTGAGC              GC^T[ACGT]AGC
             1849  GCTAAGC             
BsaAI        1173  CACGTG               [CT]AC^GT[GA]
             2356  CACGTG              
BsaI         4057  GAGACC               GGTCTC(1/5)
             3183  GGTCTC              
BseAI         368  TCCGGA               T^CCGGA
             3583  TCCGGA              
BsmBI        5054  CGTCTC               CGTCTC(1/5)
             5572  GAGACG              
BsmI         1404  GAATGC               GAATGC(1/-1)
             3564  GAATGC              
Bsp1407I     5228  TGTACA               T^GTACA
             5348  TGTACA              
BspEI         368  TCCGGA               T^CCGGA
             3583  TCCGGA              
BspLU11I       97  ACATGT               A^CATGT
             2615  ACATGT              
BsrGI        5228  TGTACA               T^GTACA
             5348  TGTACA              
Bst1107I      197  GTATAC               GTA^TAC
             3412  GTATAC              
BstXI         624  CCACTGCGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2838  CCACCCATTTGG        
BstZ17I       197  GTATAC               GTA^TAC
             3412  GTATAC              
CelII         484  GCTGAGC              GC^T[ACGT]AGC
             1849  GCTAAGC             
DrdI         2971  GACATCCATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5046  GACCCGCACGTC        
Eco47III     1993  AGCGCT               AGC^GCT
             2908  AGCGCT              
KasI           31  GGCGCC               G^GCGCC
             1826  GGCGCC              
MroI          368  TCCGGA               T^CCGGA
             3583  TCCGGA              
NarI           31  GGCGCC               GG^CGCC
             1826  GGCGCC              
NcoI         1688  CCATGG               C^CATGG
             2603  CCATGG              
PaeR7I       1596  CTCGAG               C^TCGAG
             3942  CTCGAG              
PmaCI        1173  CACGTG               CAC^GTG
             2356  CACGTG              
PmlI         1173  CACGTG               CAC^GTG
             2356  CACGTG              
SapI         5593  GAAGAGC              GCTCTTC(1/4)
             4068  GCTCTTC             
ScaI         2553  AGTACT               AGT^ACT
             4389  AGTACT              
SfoI           31  GGCGCC               GGC^GCC
             1826  GGCGCC              
SspBI        5228  TGTACA               T^GTACA
             5348  TGTACA              
StuI         1739  AGGCCT               AGG^CCT
             3882  AGGCCT              
XhoI         1596  CTCGAG               C^TCGAG
             3942  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  136 enzymes) #
#########################################

Acc65I      AccI        AccII       AciI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
Asp700I     Asp718I     AspHI       AvaI        AvaII       
AviII       BalI        BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaBI       BsaHI       BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrDI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        KpnI        Ksp632I     MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PshAI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI        
XmnI        
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