Restriction map for pF1KA0816
 (Linear) (Six-base) MAPSORT of: KIAA0816.seq  Check: 3005  from: 1  to: 5706



KIAA0816.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1344    5706 
   Size:       1344    4362
 
AccI GT'mk_AC

Cuts at:      0     198    1519    3007    3263    3413    5706 
   Size:        198    1321    1488     256     150    2293

  Fragments arranged by size: 

                2293    1488    1321     256     198     150
 
Acc65I G'GTAC_C

Cuts at:      0    1696    3219    4439    4953    5706 
   Size:       1696    1523    1220     514     753

  Fragments arranged by size: 

                1696    1523    1220     753     514
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    4042    4300    5706 
   Size:       4042     258    1406

  Fragments arranged by size: 

                4042    1406     258
 
AfeI AGC'GCT

Cuts at:      0    1995    2910    5706 
   Size:       1995     915    2796

  Fragments arranged by size: 

                2796    1995     915
 
AflIII A'CryG_T

Cuts at:      0      97    1172    2357    2615    5706 
   Size:         97    1075    1185     258    3091

  Fragments arranged by size: 

                3091    1185    1075     258      97
 
AgeI A'CCGG_T

Cuts at:      0    3750    5706 
   Size:       3750    1956
 
AhdI GACnn_n'nnGTC

Cuts at:      0    5532    5706 
   Size:       5532     174
 
AleI CACnn'nnGTG

Cuts at:      0    2204    4126    4207    5706 
   Size:       2204    1922      81    1499

  Fragments arranged by size: 

                2204    1922    1499      81
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3121    5706 
   Size:       3121    2585
 
AlwNI CAG_nnn'CTG

Cuts at:      0     951    1318    1359    1656    5706 
   Size:        951     367      41     297    4050

  Fragments arranged by size: 

                4050     951     367     297      41
 
ApaI G_GGCC'C

Cuts at:      0    1629    5706 
   Size:       1629    4077
 
ApaLI G'TGCA_C

Cuts at:      0    5111    5706 
   Size:       5111     595
 
ApoI r'AATT_y

Cuts at:      0    2002    2782    5706 
   Size:       2002     780    2924

  Fragments arranged by size: 

                2924    2002     780
 
AvaI C'yCGr_G

Cuts at:      0      67     320    1596    1667    2345    2582    3506    3942 
   Size:         67     253    1276      71     678     237     924     436

Cuts at:   3942    5706 
   Size:       1764

  Fragments arranged by size: 

                1764    1276     924     678     436     253     237      71
                  67
 
AvrII C'CTAG_G

Cuts at:      0    3627    4966    5706 
   Size:       3627    1339     740

  Fragments arranged by size: 

                3627    1339     740
 
BamHI G'GATC_C

Cuts at:      0    1472    5706 
   Size:       1472    4234
 
BanI G'GyrC_C

Cuts at:      0      31    1591    1696    1826    3219    3724    4439    4953 
   Size:         31    1560     105     130    1393     505     715     514

Cuts at:   4953    5706 
   Size:        753

  Fragments arranged by size: 

                1560    1393     753     715     514     505     130     105
                  31
 
BanII G_rGCy'C

Cuts at:      0    1537    1629    2452    2644    3386    4151    4996    5706 
   Size:       1537      92     823     192     742     765     845     710

  Fragments arranged by size: 

                1537     845     823     765     742     710     192      92
            
 
BbeI G_GCGC'C

Cuts at:      0      35    1830    5706 
   Size:         35    1795    3876

  Fragments arranged by size: 

                3876    1795      35
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1437    1955    2779    2973    4042    4288    5527    5706 
   Size:       1437     518     824     194    1069     246    1239     179

  Fragments arranged by size: 

                1437    1239    1069     824     518     246     194     179
            
 
BbvCI CC'TCA_GC

Cuts at:      0     549    4814    5706 
   Size:        549    4265     892

  Fragments arranged by size: 

                4265     892     549
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2427    5706 
   Size:       2427    3279
 
BclI T'GATC_A

Cuts at:      0    4394    5706 
   Size:       4394    1312
 
BfrBI ATG'CAT

Cuts at:      0    5516    5706 
   Size:       5516     190
 
BglI GCCn_nnn'nGGC

Cuts at:      0     356    1145    1287    1541    2177    2456    3095    4010 
   Size:        356     789     142     254     636     279     639     915

Cuts at:   4010    5706 
   Size:       1696

  Fragments arranged by size: 

                1696     915     789     639     636     356     279     254
                 142
 
BlpI GC'TnA_GC

Cuts at:      0     485    1850    5706 
   Size:        485    1365    3856

  Fragments arranged by size: 

                3856    1365     485
 
Bme1580I G_kGCm'C

Cuts at:      0    1098    1172    1629    2211    3915    5115    5706 
   Size:       1098      74     457     582    1704    1200     591

  Fragments arranged by size: 

                1704    1200    1098     591     582     457      74
 
BmgBI CAC'GTC

Cuts at:      0    2313    5054    5706 
   Size:       2313    2741     652

  Fragments arranged by size: 

                2741    2313     652
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     135    1532    1725    1848    2138    2346    2640    2763 
   Size:        135    1397     193     123     290     208     294     123

Cuts at:   2763    2995    3564    4317    4467    4590    4990    5706 
   Size:        232     569     753     150     123     400     716

  Fragments arranged by size: 

                1397     753     716     569     400     294     290     232
                 208     193     150     135     123     123     123
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     312    5706 
   Size:        312    5394
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598    1420    1549    1678    1728    4059    5080    5126 
   Size:        598     822     129     129      50    2331    1021      46

Cuts at:   5126    5618    5706 
   Size:        492      88

  Fragments arranged by size: 

                2331    1021     822     598     492     129     129      88
                  50      46
 
Bpu10I CC'TnA_GC

Cuts at:      0     549    4145    4814    5706 
   Size:        549    3596     669     892

  Fragments arranged by size: 

                3596     892     669     549
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    5452    5706 
   Size:       5452     254
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3189    4051    5706 
   Size:       3189     862    1655

  Fragments arranged by size: 

                3189    1655     862
 
BsaAI yAC'GTr

Cuts at:      0    1175    2358    5706 
   Size:       1175    1183    3348

  Fragments arranged by size: 

                3348    1183    1175
 
BsaBI GATnn'nnATC

Cuts at:      0    1784    2251    4592    5413    5706 
   Size:       1784     467    2341     821     293

  Fragments arranged by size: 

                2341    1784     821     467     293
 
BsaHI Gr'CG_yC

Cuts at:      0      32    1764    1827    2762    4360    5706 
   Size:         32    1732      63     935    1598    1346

  Fragments arranged by size: 

                1732    1598    1346     935      63      32
 
BsaWI w'CCGG_w

Cuts at:      0     368    1469    1631    2513    3437    3583    3750    4601 
   Size:        368    1101     162     882     924     146     167     851

Cuts at:   4601    5706 
   Size:       1105

  Fragments arranged by size: 

                1105    1101     924     882     851     368     167     162
                 146
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     325     355     574     604     661     691     694     724 
   Size:        325      30     219      30      57      30       3      30

Cuts at:    724    1501    1531    1878    1908    2326    2356    5569    5599 
   Size:        777      30     347      30     418      30    3213      30

Cuts at:   5599    5706 
   Size:        107

  Fragments arranged by size: 

                3213     777     418     347     325     219     107      57
                  30      30      30      30      30      30      30      30
                   3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     346     550     595     666    1078    1216    1522    5077 
   Size:        346     204      45      71     412     138     306    3555

Cuts at:   5077    5173    5488    5679    5706 
   Size:         96     315     191      27

  Fragments arranged by size: 

                3555     412     346     315     306     204     191     138
                  96      71      45      27
 
BseYI C'CCAG_C

Cuts at:      0    2075    2451    2741    5100    5706 
   Size:       2075     376     290    2359     606

  Fragments arranged by size: 

                2359    2075     606     376     290
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     777     905    1147    1209    2109    3289    3775 
   Size:        122     655     128     242      62     900    1180     486

Cuts at:   3775    4845    5706 
   Size:       1070     861

  Fragments arranged by size: 

                1180    1070     900     861     655     486     242     128
                 122      62
 
BsiEI CG_ry'CG

Cuts at:      0    1287    5706 
   Size:       1287    4419
 
BsiHKAI G_wGCw'C

Cuts at:      0     114     445    3614    4151    5115    5706 
   Size:        114     331    3169     537     964     591

  Fragments arranged by size: 

                3169     964     591     537     331     114
 
BsmI GAATG_Cn'

Cuts at:      0    1410    3570    5706 
   Size:       1410    2160    2136

  Fragments arranged by size: 

                2160    2136    1410
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    5060    5566    5706 
   Size:       5060     506     140

  Fragments arranged by size: 

                5060     506     140
 
Bsp1286I G_dGCh'C

Cuts at:      0     114     445    1098    1172    1537    1629    2211    2452 
   Size:        114     331     653      74     365      92     582     241

Cuts at:   2452    2644    3386    3614    3915    4151    4996    5115    5706 
   Size:        192     742     228     301     236     845     119     591

  Fragments arranged by size: 

                 845     742     653     591     582     365     331     301
                 241     236     228     192     119     114      92      74
            
 
BspEI T'CCGG_A

Cuts at:      0     368    3583    5706 
   Size:        368    3215    2123

  Fragments arranged by size: 

                3215    2123     368
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     136    1144    1344    2094    2494    3094    3113    3133 
   Size:        136    1008     200     750     400     600      19      20

Cuts at:   3133    3884    4073    4629    5706 
   Size:        751     189     556    1077

  Fragments arranged by size: 

                1077    1008     751     750     600     556     400     200
                 189     136      20      19
 
BsrBI CCG'CTC

Cuts at:      0     257     695    1370    3579    4506    5706 
   Size:        257     438     675    2209     927    1200

  Fragments arranged by size: 

                2209    1200     927     675     438     257
 
BsrDI GCAATG_nn'

Cuts at:      0     479    1080    1409    1673    1772    3248    3858    4415 
   Size:        479     601     329     264      99    1476     610     557

Cuts at:   4415    4466    5706 
   Size:         51    1240

  Fragments arranged by size: 

                1476    1240     610     601     557     479     329     264
                  99      51
 
BsrFI r'CCGG_y

Cuts at:      0    1046    1151    1286    2764    2915    2993    3750    5706 
   Size:       1046     105     135    1478     151      78     757    1956

  Fragments arranged by size: 

                1956    1478    1046     757     151     135     105      78
            
 
BsrGI T'GTAC_A

Cuts at:      0    5228    5348    5706 
   Size:       5228     120     358

  Fragments arranged by size: 

                5228     358     120
 
BssHII G'CGCG_C

Cuts at:      0      17    5706 
   Size:         17    5689
 
BssSI C'ACGA_G

Cuts at:      0    3862    5706 
   Size:       3862    1844
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     109    1359    3125    5706 
   Size:        109    1250    1766    2581

  Fragments arranged by size: 

                2581    1766    1250     109
 
BstEII G'GTnAC_C

Cuts at:      0    2475    5706 
   Size:       2475    3231
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     631    2845    5706 
   Size:        631    2214    2861

  Fragments arranged by size: 

                2861    2214     631
 
BstYI r'GATC_y

Cuts at:      0    1472    4574    4694    5706 
   Size:       1472    3102     120    1012

  Fragments arranged by size: 

                3102    1472    1012     120
 
BstZ17I GTA'TAC

Cuts at:      0     199    3414    5706 
   Size:        199    3215    2292

  Fragments arranged by size: 

                3215    2292     199
 
Bsu36I CC'TnA_GG

Cuts at:      0    2673    5706 
   Size:       2673    3033
 
BtgI C'CryG_G

Cuts at:      0    1688    2603    4080    5706 
   Size:       1688     915    1477    1626

  Fragments arranged by size: 

                1688    1626    1477     915
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     197     401     431     644     764     911    5706 
   Size:        197     204      30     213     120     147    4795

  Fragments arranged by size: 

                4795     213     204     197     147     120      30
 
BtsI GCAGTG_nn'

Cuts at:      0      16     110     154     381     430     560     624    1130 
   Size:         16      94      44     227      49     130      64     506

Cuts at:   1130    1135    1381    2325    3301    4307    5706 
   Size:          5     246     944     976    1006    1399

  Fragments arranged by size: 

                1399    1006     976     944     506     246     227     130
                  94      64      49      44      16       5
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     384     419     990    1025    4672    4707    4821    4856 
   Size:        384      35     571      35    3647      35     114      35

Cuts at:   4856    5706 
   Size:        850

  Fragments arranged by size: 

                3647     850     571     384     114      35      35      35
                  35
 
DraIII CAC_nnn'GTG

Cuts at:      0    3016    5706 
   Size:       3016    2690
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2977    5052    5706 
   Size:       2977    2075     654

  Fragments arranged by size: 

                2977    2075     654
 
EaeI y'GGCC_r

Cuts at:      0      56    1610    2148    2462    3402    4313    4650    5706 
   Size:         56    1554     538     314     940     911     337    1056

  Fragments arranged by size: 

                1554    1056     940     911     538     337     314      56
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     896    1795    2700    4075    4450    5588    5706 
   Size:        896     899     905    1375     375    1138     118

  Fragments arranged by size: 

                1375    1138     905     899     896     375     118
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     241     838    5706 
   Size:        241     597    4868

  Fragments arranged by size: 

                4868     597     241
 
EcoICRI GAG'CTC

Cuts at:      0    4149    5706 
   Size:       4149    1557
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598    1420    1549    1678    1728    4042    4059    4300 
   Size:        598     822     129     129      50    2314      17     241

Cuts at:   4300    5080    5126    5618    5706 
   Size:        780      46     492      88

  Fragments arranged by size: 

                2314     822     780     598     492     241     129     129
                  88      50      46      17
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1542    1625    1914    2280    2738    3056    4512    5380 
   Size:       1542      83     289     366     458     318    1456     868

Cuts at:   5380    5493    5706 
   Size:        113     213

  Fragments arranged by size: 

                1542    1456     868     458     366     318     289     213
                 113      83
 
EcoRV GAT'ATC

Cuts at:      0    4797    5706 
   Size:       4797     909
 
FspI TGC'GCA

Cuts at:      0      45     436    2317    4592    5706 
   Size:         45     391    1881    2275    1114

  Fragments arranged by size: 

                2275    1881    1114     391      45
 
FspAI rTGC'GCAy

Cuts at:      0    4592    5706 
   Size:       4592    1114
 
HaeII r_GCGC'y

Cuts at:      0      35     565     747     864     969    1830    1997    2082 
   Size:         35     530     182     117     105     861     167      85

Cuts at:   2082    2912    5706 
   Size:        830    2794

  Fragments arranged by size: 

                2794     861     830     530     182     167     117     105
                  85      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     593     625     661     693     760     792    1501    1533 
   Size:        593      32      36      32      67      32     709      32

Cuts at:   1533    1754    1786    1860    1892    2269    2301    2326    2358 
   Size:        221      32      74      32     377      32      25      32

Cuts at:   2358    2722    2754    2855    2887    4029    4061    4729    4761 
   Size:        364      32     101      32    1142      32     668      32

Cuts at:   4761    5144    5176    5518    5550    5706 
   Size:        383      32     342      32     156

  Fragments arranged by size: 

                1142     709     668     593     383     377     364     342
                 221     156     101      74      67      36      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      25
 
HincII GTy'rAC

Cuts at:      0    1002    1080    4737    5706 
   Size:       1002      78    3657     969

  Fragments arranged by size: 

                3657    1002     969      78
 
HpaI GTT'AAC

Cuts at:      0    1080    5706 
   Size:       1080    4626
 
KasI G'GCGC_C

Cuts at:      0      31    1826    5706 
   Size:         31    1795    3880

  Fragments arranged by size: 

                3880    1795      31
 
KpnI G_GTAC'C

Cuts at:      0    1700    3223    4443    4957    5706 
   Size:       1700    1523    1220     514     749

  Fragments arranged by size: 

                1700    1523    1220     749     514
 
MfeI C'AATT_G

Cuts at:      0    2777    5706 
   Size:       2777    2929
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     470     937    3237    3896    5706 
   Size:         64     406     467    2300     659    1810

  Fragments arranged by size: 

                2300    1810     659     467     406      64
 
MscI TGG'CCA

Cuts at:      0      58    1612    2150    2464    4315    5706 
   Size:         58    1554     538     314    1851    1391

  Fragments arranged by size: 

                1851    1554    1391     538     314      58
 
MslI CAynn'nnrTG

Cuts at:      0     182     386     629     802    1939    2204    2593    3178 
   Size:        182     204     243     173    1137     265     389     585

Cuts at:   3178    3235    3764    3862    3892    4124    4126    4207    5706 
   Size:         57     529      98      30     232       2      81    1499

  Fragments arranged by size: 

                1499    1137     585     529     389     265     243     232
                 204     182     173      98      81      57      30       2
            
 
MspA1I CmG'CkG

Cuts at:      0     461     827     842    1295    2102    2455    3080    4020 
   Size:        461     366      15     453     807     353     625     940

Cuts at:   4020    4818    5358    5583    5706 
   Size:        798     540     225     123

  Fragments arranged by size: 

                 940     807     798     625     540     461     453     366
                 353     225     123      15
 
NaeI GCC'GGC

Cuts at:      0    1048    2766    2995    5706 
   Size:       1048    1718     229    2711

  Fragments arranged by size: 

                2711    1718    1048     229
 
NarI GG'CG_CC

Cuts at:      0      32    1827    5706 
   Size:         32    1795    3879

  Fragments arranged by size: 

                3879    1795      32
 
NcoI C'CATG_G

Cuts at:      0    1688    2603    5706 
   Size:       1688     915    3103

  Fragments arranged by size: 

                3103    1688     915
 
NdeI CA'TA_TG

Cuts at:      0    3765    5706 
   Size:       3765    1941
 
NgoMIV G'CCGG_C

Cuts at:      0    1046    2764    2993    5706 
   Size:       1046    1718     229    2713

  Fragments arranged by size: 

                2713    1718    1046     229
 
NsiI A_TGCA'T

Cuts at:      0    5518    5706 
   Size:       5518     188
 
NspI r_CATG'y

Cuts at:      0     101     435    2619    3897    4135    5706 
   Size:        101     334    2184    1278     238    1571

  Fragments arranged by size: 

                2184    1571    1278     334     238     101
 
PasI CC'CwG_GG

Cuts at:      0    1537    5706 
   Size:       1537    4169
 
PciI A'CATG_T

Cuts at:      0      97    2615    5706 
   Size:         97    2518    3091

  Fragments arranged by size: 

                3091    2518      97
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1706    2471    3531    5706 
   Size:       1706     765    1060    2175

  Fragments arranged by size: 

                2175    1706    1060     765
 
PfoI T'CCnGG_A

Cuts at:      0    4214    5259    5501    5706 
   Size:       4214    1045     242     205

  Fragments arranged by size: 

                4214    1045     242     205
 
PmlI CAC'GTG

Cuts at:      0    1175    2358    5706 
   Size:       1175    1183    3348

  Fragments arranged by size: 

                3348    1183    1175
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     532     564    5669    5701    5706 
   Size:        532      32    5105      32       5

  Fragments arranged by size: 

                5105     532      32      32       5
 
PpuMI rG'GwC_Cy

Cuts at:      0    2280    2738    3056    4512    5706 
   Size:       2280     458     318    1456    1194

  Fragments arranged by size: 

                2280    1456    1194     458     318
 
PshAI GACnn'nnGTC

Cuts at:      0    2255    2371    4340    5706 
   Size:       2255     116    1969    1366

  Fragments arranged by size: 

                2255    1969    1366     116
 
PspOMI G'GGCC_C

Cuts at:      0    1625    5706 
   Size:       1625    4081
 
PspXI vC'TCGA_Gb

Cuts at:      0    1596    5706 
   Size:       1596    4110
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    5263    5295    5706 
   Size:       5263      32     411

  Fragments arranged by size: 

                5263     411      32
 
PstI C_TGCA'G

Cuts at:      0    2091    2948    5628    5706 
   Size:       2091     857    2680      78

  Fragments arranged by size: 

                2680    2091     857      78
 
PvuII CAG'CTG

Cuts at:      0    1295    2455    3080    4020    5358    5583    5706 
   Size:       1295    1160     625     940    1338     225     123

  Fragments arranged by size: 

                1338    1295    1160     940     625     225     123
 
RsrII CG'GwC_CG

Cuts at:      0    3581    5706 
   Size:       3581    2125
 
SacI G_AGCT'C

Cuts at:      0    4151    5706 
   Size:       4151    1555
 
SanDI GG'GwC_CC

Cuts at:      0    2738    5706 
   Size:       2738    2968
 
SapI GCTCTTCn'nnn_

Cuts at:      0    4075    5588    5706 
   Size:       4075    1513     118

  Fragments arranged by size: 

                4075    1513     118
 
SbfI CC_TGCA'GG

Cuts at:      0    2091    5706 
   Size:       2091    3615
 
ScaI AGT'ACT

Cuts at:      0    2555    4391    5706 
   Size:       2555    1836    1315

  Fragments arranged by size: 

                2555    1836    1315
 
SfcI C'TryA_G

Cuts at:      0     462    2087    2944    3036    3367    5280    5624    5706 
   Size:        462    1625     857      92     331    1913     344      82

  Fragments arranged by size: 

                1913    1625     857     462     344     331      92      82
            
 
SfoI GGC'GCC

Cuts at:      0      33    1828    5706 
   Size:         33    1795    3878

  Fragments arranged by size: 

                3878    1795      33
 
SgrAI Cr'CCGG_yG

Cuts at:      0    3750    5706 
   Size:       3750    1956
 
SmaI CCC'GGG

Cuts at:      0     322    1669    2347    3508    5706 
   Size:        322    1347     678    1161    2198

  Fragments arranged by size: 

                2198    1347    1161     678     322
 
SmlI C'TyrA_G

Cuts at:      0    1596    3942    5467    5706 
   Size:       1596    2346    1525     239

  Fragments arranged by size: 

                2346    1596    1525     239
 
StuI AGG'CCT

Cuts at:      0    1741    3884    5706 
   Size:       1741    2143    1822

  Fragments arranged by size: 

                2143    1822    1741
 
StyI C'CwwG_G

Cuts at:      0     938    1688    2603    3627    4966    5399    5706 
   Size:        938     750     915    1024    1339     433     307

  Fragments arranged by size: 

                1339    1024     938     915     750     433     307
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     252    1597    1830    2855    3776    4299    4572    4879 
   Size:        252    1345     233    1025     921     523     273     307

Cuts at:   4879    5706 
   Size:        827

  Fragments arranged by size: 

                1345    1025     921     827     523     307     273     252
                 233
 
TatI w'GTAC_w

Cuts at:      0    1376    1835    1958    2360    2489    2553    3284    3542 
   Size:       1376     459     123     402     129      64     731     258

Cuts at:   3542    4325    4389    5228    5348    5706 
   Size:        783      64     839     120     358

  Fragments arranged by size: 

                1376     839     783     731     459     402     358     258
                 129     123     120      64      64
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     574     606     694     726    1464    1496    2408    2440 
   Size:        574      32      88      32     738      32     912      32

Cuts at:   2440    3303    3335    3635    3667    4026    4058    4596    4628 
   Size:        863      32     300      32     359      32     538      32

Cuts at:   4628    5227    5259    5706 
   Size:        599      32     447

  Fragments arranged by size: 

                 912     863     738     599     574     538     447     359
                 300      88      32      32      32      32      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    4719    5706 
   Size:       4719     987
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     595    4293    5548    5663    5706 
   Size:        595    3698    1255     115      43

  Fragments arranged by size: 

                3698    1255     595     115      43
 
XhoI C'TCGA_G

Cuts at:      0    1596    3942    5706 
   Size:       1596    2346    1764

  Fragments arranged by size: 

                2346    1764    1596
 
XmaI C'CCGG_G

Cuts at:      0     320    1667    2345    3506    5706 
   Size:        320    1347     678    1161    2200

  Fragments arranged by size: 

                2200    1347    1161     678     320
 
XmnI GAAnn'nnTTC

Cuts at:      0    2027    3145    5153    5706 
   Size:       2027    1118    2008     553

  Fragments arranged by size: 

                2027    2008    1118     553
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI    CspCI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRV     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MfeI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI    PvuII    RsrII     SacI    SanDI     SapI
     SbfI     ScaI     SfcI     SfoI    SgrAI     SmaI     SmlI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AloI     AscI     AseI    AsiSI     BaeI
     BcgI    BglII     BmtI    BsiWI    BspHI    BstBI     ClaI     DraI
     EagI    EcoNI    EcoRI     FalI     FseI  HindIII     MluI     NheI
     NotI     NruI     PacI     PmeI     PsiI     PvuI    SacII     SalI
    SexAI     SfiI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     XbaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com