Restriction map for pF1KA0804
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# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AflII       
AgeI        Alw44I      ApaI        ApaLI       AscI        
Asp718I     AvaI        AviII       AvrII       BbeI        
BbrPI       BcgI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsiEI       BsiWI       BsoBI       
BspDI       BsrBI       BsrFI       BssHII      Bst1107I    
BstBI       BstUI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DrdI        EagI        EclXI       
Eco52I      EcoNI       EcoT22I     FauI        FseI        
FspI        HpaI        KasI        KpnI        KspI        
MluI        MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacII       SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    StuI        SwaI        XhoI        XmaCI       
XmaI        


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# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccIII       2684  TCCGGA               T^CCGGA
AcyI         3794  GACGCC               G[GA]^CG[CT]C
AflIII       1164  ACATGT               A^C[GA][CT]GT
AhdI         2618  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2299  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1953  AGCGCT               AGC^GCT
AseI         1935  ATTAAT               AT^TAAT
AsnI         1935  ATTAAT               AT^TAAT
AspEI        2618  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          554  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII        1670  GAGCTC               G[GA]GC[CT]^C
BbsI         3014  GTCTTC               GAAGAC(2/6)
BbvCI        1482  GCTGAGG              CCTCAGC(-5/-2)
BciVI         484  GTATCC               GTATCC(6/5)
BcnI         2833  CCCGG                CC^[GC]GG
BpuAI        3014  GTCTTC               GAAGAC(2/6)
BsaAI        2774  TACGTG               [CT]AC^GT[GA]
BsaHI        3794  GACGCC               G[GA]^CG[CT]C
BsaI         3358  GAGACC               GGTCTC(1/5)
BseAI        2684  TCCGGA               T^CCGGA
BsgI         2843  GTGCAG               GTGCAG(16/14)
Bsp1407I     1962  TGTACA               T^GTACA
BspEI        2684  TCCGGA               T^CCGGA
BspHI        3198  TCATGA               T^CATGA
BspLU11I     1164  ACATGT               A^CATGT
BsrGI        1962  TGTACA               T^GTACA
BssSI        2896  CTCGTG               CACGAG(-5/-1)
BstEII       3732  GGTCACC              G^GT[ACGT]ACC
BstPI        3732  GGTCACC              G^GT[ACGT]ACC
BstXI        2940  CCACTTTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2299  CCTGAGG              CC^T[ACGT]AGG
DraIII        663  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          950  CCATGG               C^C[GA][CT]GG
Eam1105I     2618  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1953  AGCGCT               AGC^GCT
Eco81I       2299  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      3732  GGTCACC              G^GT[ACGT]ACC
EcoRV        1972  GATATC               GAT^ATC
HgaI         3794  GACGC                GACGC(5/10)
Hin1I        3794  GACGCC               G[GA]^CG[CT]C
HincII       2675  GTCGAC               GT[CT]^[GA]AC
HindII       2675  GTCGAC               GT[CT]^[GA]AC
MfeI          476  CAATTG               C^AATTG
MroI         2684  TCCGGA               T^CCGGA
MunI          476  CAATTG               C^AATTG
NciI         2833  CCCGG                CC^[GC]GG
NcoI          950  CCATGG               C^CATGG
NdeI         1966  CATATG               CA^TATG
PflFI         554  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        1935  ATTAAT               AT^TAAT
RcaI         3198  TCATGA               T^CATGA
SacI         1670  GAGCTC               GAGCT^C
SalI         2675  GTCGAC               G^TCGAC
SapI           61  GAAGAGC              GCTCTTC(1/4)
SpeI          571  ACTAGT               A^CTAGT
SspBI        1962  TGTACA               T^GTACA
Tth111I       554  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BanI         1734  GGTGCC               G^G[CT][GA]CC
             3244  GGTGCC              
BsaBI         535  GATCAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              709  GATGCTCATC          
BsaWI        1634  ACCGGA               [AT]^CCGG[AT]
             2684  TCCGGA              
BsmBI        4108  CGTCTC               CGTCTC(1/5)
             3792  GAGACG              
BstAPI       2194  GCAGGTCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3538  GCATTTATTGC         
DraI         2389  TTTAAA               TTT^AAA
             3522  TTTAAA              
EarI           61  GAAGAG               CTCTTC(1/4)
             2668  GAAGAG              
HaeII         592  GGCGCT               [GA]GCGC^[CT]
             1953  AGCGCT              
HindIII        89  AAGCTT               A^AGCTT
              545  AAGCTT              
Ksp632I        61  GAAGAG               CTCTTC(1/4)
             2668  GAAGAG              
MaeII        2775  ACGT                 A^CGT
             3408  ACGT                
MamI          535  GATCAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              709  GATGCTCATC          
PpuMI        2567  AGGTCCT              [GA]G^G[AT]CC[CT]
             2624  AGGTCCT             
SexAI        2252  ACCAGGT              A^CC[AT]GGT
             2984  ACCAGGT             
SspI          528  AATATT               AAT^ATT
              732  AATATT              
TaqI         1668  TCGA                 T^CGA
             2676  TCGA                
TthHB8I      1668  TCGA                 T^CGA
             2676  TCGA                
XbaI           84  TCTAGA               T^CTAGA
              844  TCTAGA              
XcmI          665  CCATGTGGGATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1048  CCACTGCTGGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BalI        BamHI       BbvI        BclI        
BfaI        BglII       BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EcoO109I    EcoRI       EcoRII      
EcoT14I     FbaI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Van91I      XhoII       
XmnI        
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