Restriction map for pF1KA0773
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AcsI        
AgeI        Alw44I      Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspHI       AspI        AvaI        AvrII       BbeI        
BbrPI       BcgI        BclI        BglII       BlnI        
BlpI        Bpu1102I    BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HaeII       HincII      HindII      HpaI        KasI        
KpnI        KspI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tsp509I     Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        1024  GACGTC               GACGT^C
AccI          996  GTCTAC               GT^[AC][GT]AC
AfaI          669  GTAC                 GT^AC
AflII         211  CTTAAG               C^TTAAG
AflIII        653  ACGTGT               A^C[GA][CT]GT
AhdI          958  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         589  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI         958  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         401  TGCGCA               TGC^GCA
BalI          248  TGGCCA               TGG^CCA
BamHI         736  GGATCC               G^GATCC
BanI          924  GGCACC               G^G[CT][GA]CC
BfaI          460  CTAG                 C^TAG
BfrI          211  CTTAAG               C^TTAAG
BglI          721  GCCTCTCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         652  TACGTG               [CT]AC^GT[GA]
BsaBI          67  GATGTCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI         848  GAGAC                GTCTC(1/5)
BsrBI        1001  CCGCTC               CCGCTC(-3/-3)
BsrDI         717  CATTGC               GCAATG(2/0)
BsrI          857  ACTGG                ACTGG(1/-1)
BssSI         977  CACGAG               CACGAG(-5/-1)
Eam1105I      958  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI          946  GCGGG                CCCGC(4/6)
FspI          401  TGCGCA               TGC^GCA
HgaI          467  GCGTC                GACGC(5/10)
HindIII       208  AAGCTT               A^AGCTT
MaeI          460  CTAG                 C^TAG
MamI           67  GATGTCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         248  TGGCCA               TGG^CCA
MscI          248  TGGCCA               TGG^CCA
MseI          212  TTAA                 T^TAA
MspA1I        368  CAGCTG               C[AC]G^C[GT]G
PvuII         368  CAGCTG               CAG^CTG
RsaI          669  GTAC                 GT^AC
SfcI          573  CTACAG               C^T[GA][CT]AG
Tru9I         212  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AciI         1001  CCGC                 CCGC(-3/-1)
              946  GCGG                
AcyI          466  GGCGTC               G[GA]^CG[CT]C
             1024  GACGTC              
AocI          928  CCTGAGG              CC^T[ACGT]AGG
             1034  CCTCAGG             
ApaI          242  GGGCCC               GGGCC^C
              894  GGGCCC              
BbvCI         429  CCTCAGC              CCTCAGC(-5/-2)
              901  GCTGAGG             
BciVI         408  GGATAC               GTATCC(6/5)
              811  GGATAC              
BpmI          823  CTGGAG               CTGGAG(16/14)
              961  CTGGAG              
BsaHI         466  GGCGTC               G[GA]^CG[CT]C
             1024  GACGTC              
BsgI          103  CTGCAC               GTGCAG(16/14)
             1042  GTGCAG              
BstXI         305  CCACAGCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              497  CCACACTCATGG        
BstYI         269  GGATCT               [GA]^GATC[CT]
              736  GGATCC              
Bsu36I        928  CCTGAGG              CC^T[ACGT]AGG
             1034  CCTCAGG             
CfoI          402  GCGC                 GCG^C
              875  GCGC                
EarI          912  GAAGAG               CTCTTC(1/4)
              937  GAAGAG              
Eco81I        928  CCTGAGG              CC^T[ACGT]AGG
             1034  CCTCAGG             
HhaI          402  GCGC                 GCG^C
              875  GCGC                
Hin1I         466  GGCGTC               G[GA]^CG[CT]C
             1024  GACGTC              
HinP1I        402  GCGC                 G^CGC
              875  GCGC                
HphI           36  GGTGA                GGTGA(8/7)
              526  GGTGA               
Ksp632I       912  GAAGAG               CTCTTC(1/4)
              937  GAAGAG              
MflI          269  GGATCT               [GA]^GATC[CT]
              736  GGATCC              
PpuMI         789  GGGACCT              [GA]G^G[AT]CC[CT]
              956  GGGACCT             
SmlI          211  CTTAAG               C^T[CT][GA]AG
              620  CTCAAG              
XhoII         269  GGATCT               [GA]^GATC[CT]
              736  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AluI        AlwI        AvaII       BanII       BbsI        
BbvI        BcnI        BmyI        BpuAI       BsaJI       
BseRI       BsiYI       BslI        BsmFI       Bsp1286I    
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MslI        MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
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