Restriction map for pF1KA0773
 (Linear) (Six-base) MAPSORT of: KIAA0773.seq  Check: 7287  from: 1  to: 1080



KIAA0773.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AatII G_ACGT'C

Cuts at:      0    1028    1080 
   Size:       1028      52
 
AccI GT'mk_AC

Cuts at:      0     997    1080 
   Size:        997      83
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     791    1080 
   Size:         82     709     289

  Fragments arranged by size: 

                 709     289      82
 
AflII C'TTAA_G

Cuts at:      0     211    1080 
   Size:        211     869
 
AflIII A'CryG_T

Cuts at:      0     653    1080 
   Size:        653     427
 
AhdI GACnn_n'nnGTC

Cuts at:      0     963    1080 
   Size:        963     117
 
AlwNI CAG_nnn'CTG

Cuts at:      0     594    1080 
   Size:        594     486
 
ApaI G_GGCC'C

Cuts at:      0     246     898    1080 
   Size:        246     652     182

  Fragments arranged by size: 

                 652     246     182
 
BamHI G'GATC_C

Cuts at:      0     736    1080 
   Size:        736     344
 
BanI G'GyrC_C

Cuts at:      0     924    1080 
   Size:        924     156
 
BanII G_rGCy'C

Cuts at:      0     246     864     898    1080 
   Size:        246     618      34     182

  Fragments arranged by size: 

                 618     246     182      34
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     367     506     989    1080 
   Size:        367     139     483      91

  Fragments arranged by size: 

                 483     367     139      91
 
BbvCI CC'TCA_GC

Cuts at:      0     430     902    1080 
   Size:        430     472     178

  Fragments arranged by size: 

                 472     430     178
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     402     805    1080 
   Size:        402     403     275

  Fragments arranged by size: 

                 403     402     275
 
BglI GCCn_nnn'nGGC

Cuts at:      0     727    1080 
   Size:        727     353
 
Bme1580I G_kGCm'C

Cuts at:      0     246     898    1080 
   Size:        246     652     182

  Fragments arranged by size: 

                 652     246     182
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     867    1080 
   Size:        867     213
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     844     982    1080 
   Size:        844     138      98

  Fragments arranged by size: 

                 844     138      98
 
Bpu10I CC'TnA_GC

Cuts at:      0     430     902    1080 
   Size:        430     472     178

  Fragments arranged by size: 

                 472     430     178
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     605    1080 
   Size:        605     475
 
BsaAI yAC'GTr

Cuts at:      0     654    1080 
   Size:        654     426
 
BsaBI GATnn'nnATC

Cuts at:      0      71    1080 
   Size:         71    1009
 
BsaHI Gr'CG_yC

Cuts at:      0     467    1025    1080 
   Size:        467     558      55

  Fragments arranged by size: 

                 558     467      55
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     919     922     946    1072    1080 
   Size:        919       3      24     126       8

  Fragments arranged by size: 

                 919     126      24       8       3
 
BseYI C'CCAG_C

Cuts at:      0     353     772     840    1080 
   Size:        353     419      68     240

  Fragments arranged by size: 

                 419     353     240      68
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88    1063    1080 
   Size:         88     975      17

  Fragments arranged by size: 

                 975      88      17
 
Bsp1286I G_dGCh'C

Cuts at:      0     246     864     898    1080 
   Size:        246     618      34     182

  Fragments arranged by size: 

                 618     246     182      34
 
BsrBI CCG'CTC

Cuts at:      0    1003    1080 
   Size:       1003      77
 
BsrDI GCAATG_nn'

Cuts at:      0     716    1080 
   Size:        716     364
 
BssSI C'ACGA_G

Cuts at:      0     977    1080 
   Size:        977     103
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     312     504    1080 
   Size:        312     192     576

  Fragments arranged by size: 

                 576     312     192
 
BstYI r'GATC_y

Cuts at:      0     269     736    1080 
   Size:        269     467     344

  Fragments arranged by size: 

                 467     344     269
 
Bsu36I CC'TnA_GG

Cuts at:      0     929    1035    1080 
   Size:        929     106      45

  Fragments arranged by size: 

                 929     106      45
 
BtgI C'CryG_G

Cuts at:      0     179     311     518     629    1080 
   Size:        179     132     207     111     451

  Fragments arranged by size: 

                 451     207     179     132     111
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     449    1080 
   Size:        449     631
 
BtsI GCAGTG_nn'

Cuts at:      0      50     100     884    1080 
   Size:         50      50     784     196

  Fragments arranged by size: 

                 784     196      50      50
 
EaeI y'GGCC_r

Cuts at:      0     124     248     728    1080 
   Size:        124     124     480     352

  Fragments arranged by size: 

                 480     352     124     124
 
EarI CTCTTCn'nnn_

Cuts at:      0     907     932    1080 
   Size:        907      25     148

  Fragments arranged by size: 

                 907     148      25
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     791     844     982    1080 
   Size:         82     709      53     138      98

  Fragments arranged by size: 

                 709     138      98      82      53
 
EcoO109I rG'GnC_Cy

Cuts at:      0      57     242     243     790     894     895     957    1080 
   Size:         57     185       1     547     104       1      62     123

  Fragments arranged by size: 

                 547     185     123     104      62      57       1       1
            
 
FspI TGC'GCA

Cuts at:      0     403    1080 
   Size:        403     677
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     406     438     588     620     949     981    1015    1047 
   Size:        406      32     150      32     329      32      34      32

Cuts at:   1047    1080 
   Size:         33

  Fragments arranged by size: 

                 406     329     150      34      33      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     208    1080 
   Size:        208     872
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     449    1080 
   Size:        449     631
 
MscI TGG'CCA

Cuts at:      0     250    1080 
   Size:        250     830
 
MslI CAynn'nnrTG

Cuts at:      0     310     389     502     538    1080 
   Size:        310      79     113      36     542

  Fragments arranged by size: 

                 542     310     113      79      36
 
MspA1I CmG'CkG

Cuts at:      0     370    1080 
   Size:        370     710
 
NcoI C'CATG_G

Cuts at:      0     179     311     518     629    1080 
   Size:        179     132     207     111     451

  Fragments arranged by size: 

                 451     207     179     132     111
 
PasI CC'CwG_GG

Cuts at:      0     660    1080 
   Size:        660     420
 
PfoI T'CCnGG_A

Cuts at:      0      16    1080 
   Size:         16    1064
 
PpuMI rG'GwC_Cy

Cuts at:      0     790     957    1080 
   Size:        790     167     123

  Fragments arranged by size: 

                 790     167     123
 
PspOMI G'GGCC_C

Cuts at:      0     242     894    1080 
   Size:        242     652     186

  Fragments arranged by size: 

                 652     242     186
 
PvuII CAG'CTG

Cuts at:      0     370    1080 
   Size:        370     710
 
SfcI C'TryA_G

Cuts at:      0     573    1080 
   Size:        573     507
 
SmlI C'TyrA_G

Cuts at:      0     211     620    1080 
   Size:        211     409     460

  Fragments arranged by size: 

                 460     409     211
 
StyI C'CwwG_G

Cuts at:      0     179     311     518     629    1080 
   Size:        179     132     207     111     451

  Fragments arranged by size: 

                 451     207     179     132     111
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     565     642    1080 
   Size:        565      77     438

  Fragments arranged by size: 

                 565     438      77
 
TatI w'GTAC_w

Cuts at:      0     668    1080 
   Size:        668     412
 
ZraI GAC'GTC

Cuts at:      0    1026    1080 
   Size:       1026      54
 

 Enzymes that do cut:

    AatII     AccI     AcuI    AflII   AflIII     AhdI    AlwNI     ApaI
    BamHI     BanI    BanII     BbsI    BbvCI    BciVI     BglI Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BseRI
    BseYI     BsgI Bsp1286I    BsrBI    BsrDI    BssSI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EarI  Eco57MI EcoO109I
     FspI    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I     NcoI
     PasI     PfoI    PpuMI   PspOMI    PvuII     SfcI     SmlI     StyI
    TaqII     TatI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI     AgeI     AleI     AlfI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BbeI     BcgI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BplI     BsaI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com