Restriction map for pF1KA0761
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        ApaLI       AscI        Asp700I     AspEI       
AspI        AvaI        AviII       AvrII       BbeI        
BbvCI       BcgI        BclI        BfrI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaBI       
BsaHI       BsaI        BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       FbaI        FseI        
FspI        Hin1I       HpaI        KasI        KspI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NcoI        NgoMIV      
NotI        NruI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmeI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacII       SalI        SapI        SbfI        SfcI        
SfoI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I       1374  GGTACC               G^GTACC
AflIII        642  ACGTGT               A^C[GA][CT]GT
AlwNI        1641  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       993  AGCGCT               AGC^GCT
ApaI         1212  GGGCCC               GGGCC^C
AseI          478  ATTAAT               AT^TAAT
AsnI          478  ATTAAT               AT^TAAT
Asp718I      1374  GGTACC               G^GTACC
BalI         1494  TGGCCA               TGG^CCA
BbrPI        1619  CACGTG               CAC^GTG
BciVI        1401  GGATAC               GTATCC(6/5)
BcnI         1326  CCGGG                CC^[GC]GG
BsaAI        1619  CACGTG               [CT]AC^GT[GA]
BsaWI        1632  TCCGGT               [AT]^CCGG[AT]
BsmBI         639  GAGACG               CGTCTC(1/5)
BsmFI         880  GTCCC                GGGAC(10/14)
BsmI           25  GAATGC               GAATGC(1/-1)
BspMI        1177  GCAGGT               ACCTGC(4/8)
BssSI        1355  CACGAG               CACGAG(-5/-1)
BstAPI       1177  GCAGGTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         520  TTCGAA               TT^CGAA
BstXI         764  CCAAAAAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          994  GCGC                 GCG^C
DraIII        461  CACTAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         1256  GAAGAG               CTCTTC(1/4)
Eco47III      993  AGCGCT               AGC^GCT
EcoT22I       361  ATGCAT               ATGCA^T
HaeII         993  AGCGCT               [GA]GCGC^[CT]
HgaI         1345  GACGC                GACGC(5/10)
HhaI          994  GCGC                 GCG^C
HinP1I        994  GCGC                 G^CGC
HindIII       328  AAGCTT               A^AGCTT
KpnI         1374  GGTACC               GGTAC^C
Ksp632I      1256  GAAGAG               CTCTTC(1/4)
MluNI        1494  TGGCCA               TGG^CCA
MscI         1494  TGGCCA               TGG^CCA
MspA1I       1532  CAGCGG               C[AC]G^C[GT]G
NciI         1326  CCGGG                CC^[GC]GG
NdeI          614  CATATG               CA^TATG
NheI          702  GCTAGC               G^CTAGC
NsiI          361  ATGCAT               ATGCA^T
NspI           83  ACATGC               [GA]CATG^[CT]
NspV          520  TTCGAA               TT^CGAA
PacI          475  TTAATTAA             TTAAT^TAA
PmaCI        1619  CACGTG               CAC^GTG
PmlI         1619  CACGTG               CAC^GTG
PshBI         478  ATTAAT               AT^TAAT
SacI          850  GAGCTC               GAGCT^C
ScaI         1459  AGTACT               AGT^ACT
SexAI         441  ACCAGGT              A^CC[AT]GGT
SfiI         1204  GGCCAAATGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          520  TTCGAA               TT^CGAA
SpeI          858  ACTAGT               A^CTAGT
TaqI          521  TCGA                 T^CGA
TthHB8I       521  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcsI          961  GAATTT               [GA]^AATT[CT]
              986  AAATTC              
ApoI          961  GAATTT               [GA]^AATT[CT]
              986  AAATTC              
AspHI         850  GAGCTC               G[AT]GC[AT]^C
             1321  GTGCTC              
BamHI         540  GGATCC               G^GATCC
             1629  GGATCC              
BanI          445  GGTGCC               G^G[CT][GA]CC
             1374  GGTACC              
BfaI          703  CTAG                 C^TAG
              859  CTAG                
BglI         1205  GCCAAATGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1643  GCCCCTGTGGC         
BsiHKAI       850  GAGCTC               G[AT]GC[AT]^C
             1321  GTGCTC              
BstYI         540  GGATCC               [GA]^GATC[CT]
             1629  GGATCC              
Cac8I         702  GCTAGC               GC[ACGT]^[ACGT]GC
             1564  GCAGGC              
DpnII         541  GATC                 ^GATC
             1630  GATC                
DraII        1212  GGGCCCC              [GA]G^G[ACGT]CC[CT]
             1227  AGGCCCT             
DsaI         1126  CCACGG               C^C[GA][CT]GG
             1367  CCACGG              
EcoO109I     1212  GGGCCCC              [GA]G^G[ACGT]CC[CT]
             1227  AGGCCCT             
FauI         1528  CCCGC                CCCGC(4/6)
             1597  CCCGC               
HapII        1326  CCGG                 C^CGG
             1633  CCGG                
HincII        862  GTCAAC               GT[CT]^[GA]AC
             1282  GTTGAC              
HindII        862  GTCAAC               GT[CT]^[GA]AC
             1282  GTTGAC              
HpaII        1326  CCGG                 C^CGG
             1633  CCGG                
MaeI          703  CTAG                 C^TAG
              859  CTAG                
MaeIII        904  GTTAC                ^GT[ACGT]AC
              922  GTAAC               
MboI          541  GATC                 ^GATC
             1630  GATC                
MflI          540  GGATCC               [GA]^GATC[CT]
             1629  GGATCC              
MspI         1326  CCGG                 C^CGG
             1633  CCGG                
NdeII         541  GATC                 ^GATC
             1630  GATC                
Sau3AI        541  GATC                 ^GATC
             1630  GATC                
SmlI         1287  CTTGAG               C^T[CT][GA]AG
             1549  CTCAAG              
TspRI        1010  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1218  CACTG               
XhoII         540  GGATCC               [GA]^GATC[CT]
             1629  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BanII       BbsI        BbvI        BmyI        BpuAI       
BsaJI       BsiYI       BslI        BsmAI       Bsp1286I    
BspHI       BsrI        BssKI       BstF5I      BstNI       
Cfr13I      DdeI        EaeI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeII       MboII       MnlI        MseI        
MslI        MvaI        MwoI        NlaIII      NlaIV       
RcaI        RsaI        Sau96I      ScrFI       SfaNI       
StyI        TfiI        Tru9I       TseI        Tsp509I     
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