Restriction map for pF1KA0761
 (Linear) (Six-base) MAPSORT of: KIAA0761.seq  Check: 7938  from: 1  to: 1653



KIAA0761.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1168    1653 
   Size:       1168     485
 
Acc65I G'GTAC_C

Cuts at:      0    1374    1653 
   Size:       1374     279
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     746    1337    1577    1653 
   Size:         89     657     591     240      76

  Fragments arranged by size: 

                 657     591     240      89      76
 
AfeI AGC'GCT

Cuts at:      0     995    1653 
   Size:        995     658
 
AflIII A'CryG_T

Cuts at:      0     642    1653 
   Size:        642    1011
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     386    1653 
   Size:        386    1267
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1052    1084    1653 
   Size:       1052      32     569

  Fragments arranged by size: 

                1052     569      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1646    1653 
   Size:       1646       7
 
ApaI G_GGCC'C

Cuts at:      0    1216    1653 
   Size:       1216     437
 
ApoI r'AATT_y

Cuts at:      0     961     986    1653 
   Size:        961      25     667

  Fragments arranged by size: 

                 961     667      25
 
AseI AT'TA_AT

Cuts at:      0     479    1653 
   Size:        479    1174
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1358    1391    1653 
   Size:       1358      33     262

  Fragments arranged by size: 

                1358     262      33
 
BamHI G'GATC_C

Cuts at:      0     540    1629    1653 
   Size:        540    1089      24

  Fragments arranged by size: 

                1089     540      24
 
BanI G'GyrC_C

Cuts at:      0     445    1374    1653 
   Size:        445     929     279

  Fragments arranged by size: 

                 929     445     279
 
BanII G_rGCy'C

Cuts at:      0     854    1216    1315    1653 
   Size:        854     362      99     338

  Fragments arranged by size: 

                 854     362     338      99
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     266     437    1143    1653 
   Size:        266     171     706     510

  Fragments arranged by size: 

                 706     510     266     171
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1395    1653 
   Size:       1395     258
 
BfrBI ATG'CAT

Cuts at:      0     363    1653 
   Size:        363    1290
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1211    1649    1653 
   Size:       1211     438       4

  Fragments arranged by size: 

                1211     438       4
 
Bme1580I G_kGCm'C

Cuts at:      0    1216    1653 
   Size:       1216     437
 
BmgBI CAC'GTC

Cuts at:      0     643    1653 
   Size:        643    1010
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     965    1373    1653 
   Size:        965     408     280

  Fragments arranged by size: 

                 965     408     280
 
BmtI G_CTAG'C

Cuts at:      0     706    1653 
   Size:        706     947
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     140    1124    1653 
   Size:        140     984     529

  Fragments arranged by size: 

                 984     529     140
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1308    1534    1653 
   Size:       1308     226     119

  Fragments arranged by size: 

                1308     226     119
 
BsaAI yAC'GTr

Cuts at:      0    1621    1653 
   Size:       1621      32
 
BsaWI w'CCGG_w

Cuts at:      0    1632    1653 
   Size:       1632      21
 
BseYI C'CCAG_C

Cuts at:      0    1544    1653 
   Size:       1544     109
 
BsiHKAI G_wGCw'C

Cuts at:      0     854    1325    1653 
   Size:        854     471     328

  Fragments arranged by size: 

                 854     471     328
 
BsmI GAATG_Cn'

Cuts at:      0      31    1653 
   Size:         31    1622
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     633    1653 
   Size:        633    1020
 
Bsp1286I G_dGCh'C

Cuts at:      0     854    1216    1315    1325    1653 
   Size:        854     362      99      10     328

  Fragments arranged by size: 

                 854     362     328      99      10
 
BspHI T'CATG_A

Cuts at:      0      54     492     977    1653 
   Size:         54     438     485     676

  Fragments arranged by size: 

                 676     485     438      54
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1168    1653 
   Size:       1168     485
 
BssSI C'ACGA_G

Cuts at:      0    1355    1653 
   Size:       1355     298
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1183    1653 
   Size:       1183     470
 
BstBI TT'CG_AA

Cuts at:      0     521    1653 
   Size:        521    1132
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     771    1653 
   Size:        771     882
 
BstYI r'GATC_y

Cuts at:      0     540    1629    1653 
   Size:        540    1089      24

  Fragments arranged by size: 

                1089     540      24
 
BtgI C'CryG_G

Cuts at:      0    1126    1367    1653 
   Size:       1126     241     286

  Fragments arranged by size: 

                1126     286     241
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1589    1624    1653 
   Size:       1589      35      29

  Fragments arranged by size: 

                1589      35      29
 
DraIII CAC_nnn'GTG

Cuts at:      0     466    1653 
   Size:        466    1187
 
EaeI y'GGCC_r

Cuts at:      0    1123    1478    1494    1653 
   Size:       1123     355      16     159

  Fragments arranged by size: 

                1123     355     159      16
 
EarI CTCTTCn'nnn_

Cuts at:      0    1251    1653 
   Size:       1251     402
 
EcoICRI GAG'CTC

Cuts at:      0     852    1653 
   Size:        852     801
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     746    1337    1577    1653 
   Size:         89     657     591     240      76

  Fragments arranged by size: 

                 657     591     240      89      76
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1213    1228    1653 
   Size:       1213      15     425

  Fragments arranged by size: 

                1213     425      15
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     413    1653 
   Size:        413    1240
 
HaeII r_GCGC'y

Cuts at:      0     997    1653 
   Size:        997     656
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     175     207    1653 
   Size:        175      32    1446

  Fragments arranged by size: 

                1446     175      32
 
HincII GTy'rAC

Cuts at:      0     864    1284    1653 
   Size:        864     420     369

  Fragments arranged by size: 

                 864     420     369
 
HindIII A'AGCT_T

Cuts at:      0     328    1653 
   Size:        328    1325
 
KpnI G_GTAC'C

Cuts at:      0    1378    1653 
   Size:       1378     275
 
MscI TGG'CCA

Cuts at:      0    1496    1653 
   Size:       1496     157
 
MslI CAynn'nnrTG

Cuts at:      0      59      82     368     510    1653 
   Size:         59      23     286     142    1143

  Fragments arranged by size: 

                1143     286     142      59      23
 
MspA1I CmG'CkG

Cuts at:      0    1534    1653 
   Size:       1534     119
 
NdeI CA'TA_TG

Cuts at:      0     615    1653 
   Size:        615    1038
 
NheI G'CTAG_C

Cuts at:      0     702    1653 
   Size:        702     951
 
NsiI A_TGCA'T

Cuts at:      0     365    1653 
   Size:        365    1288
 
NspI r_CATG'y

Cuts at:      0      87    1653 
   Size:         87    1566
 
PacI TTA_AT'TAA

Cuts at:      0     479    1653 
   Size:        479    1174
 
PmlI CAC'GTG

Cuts at:      0    1621    1653 
   Size:       1621      32
 
PsiI TTA'TAA

Cuts at:      0     227    1653 
   Size:        227    1426
 
PspOMI G'GGCC_C

Cuts at:      0    1212    1653 
   Size:       1212     441
 
SacI G_AGCT'C

Cuts at:      0     854    1653 
   Size:        854     799
 
ScaI AGT'ACT

Cuts at:      0    1461    1653 
   Size:       1461     192
 
SexAI A'CCwGG_T

Cuts at:      0     441    1653 
   Size:        441    1212
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1211    1653 
   Size:       1211     442
 
SmlI C'TyrA_G

Cuts at:      0    1287    1549    1653 
   Size:       1287     262     104

  Fragments arranged by size: 

                1287     262     104
 
SpeI A'CTAG_T

Cuts at:      0     858    1653 
   Size:        858     795
 
StyI C'CwwG_G

Cuts at:      0     449    1412    1578    1653 
   Size:        449     963     166      75

  Fragments arranged by size: 

                 963     449     166      75
 
TatI w'GTAC_w

Cuts at:      0    1459    1653 
   Size:       1459     194
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     429     461    1653 
   Size:        429      32    1192

  Fragments arranged by size: 

                1192     429      32
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI   AflIII     AlfI     AloI    AlwNI
     ApaI     ApoI     AseI     BaeI    BamHI     BanI    BanII     BbsI
    BciVI    BfrBI     BglI Bme1580I    BmgBI     BmrI     BmtI     BplI
    BpuEI    BsaAI    BsaWI    BseYI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI
    CspCI   DraIII     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FalI
    HaeII    Hin4I   HincII  HindIII     KpnI     MscI     MslI   MspA1I
     NdeI     NheI     NsiI     NspI     PacI     PmlI     PsiI   PspOMI
     SacI     ScaI    SexAI     SfiI     SmlI     SpeI     StyI     TatI
     TstI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII     AgeI     AhdI     AleI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BbeI    BbvCI     BcgI     BclI
    BglII     BlpI     BpmI   Bpu10I     BsaI    BsaBI    BsaHI    BsaXI
    BseRI     BsgI    BsiEI    BsiWI    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
     DraI     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI     HpaI     KasI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI   NgoMIV     NotI     NruI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     SfcI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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