Restriction map for pF1KA0721
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# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BbeI        BbrPI       BciVI       
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspHI       BspLU11I    BspMI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     CelII       DraI        DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRV       EcoT22I     
FseI        FspI        Hin1I       HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MluI        MslI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        SacI        SacII       
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tth111I     Van91I      XcmI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          199  GTCGAC               GT^[AC][GT]AC
AccIII         81  TCCGGA               T^CCGGA
AfaI          831  GTAC                 GT^AC
AlwNI         305  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           71  CCTCAGG              CC^T[ACGT]AGG
AvaII          84  GGACC                G^G[AT]CC
BalI          807  TGGCCA               TGG^CCA
BamHI         192  GGATCC               G^GATCC
BanI          995  GGCACC               G^G[CT][GA]CC
BbsI          817  GAAGAC               GAAGAC(2/6)
BbvCI         825  GCTGAGG              CCTCAGC(-5/-2)
BpuAI         817  GAAGAC               GAAGAC(2/6)
BsaWI          81  TCCGGA               [AT]^CCGG[AT]
BseAI          81  TCCGGA               T^CCGGA
BsmFI         223  GTCCC                GGGAC(10/14)
BspDI         628  ATCGAT               AT^CGAT
BspEI          81  TCCGGA               T^CCGGA
BsrBI           3  GAGCGG               CCGCTC(-3/-3)
BsrFI          43  ACCGGC               [GA]^CCGG[CT]
BstAPI        304  GCAGCCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         773  TTCGAA               TT^CGAA
Bsu36I         71  CCTCAGG              CC^T[ACGT]AGG
Cfr10I         43  ACCGGC               [GA]^CCGG[CT]
ClaI          628  ATCGAT               AT^CGAT
CpoI           83  CGGACCG              CG^G[AT]CCG
DsaI          366  CCGTGG               C^C[GA][CT]GG
Eco81I         71  CCTCAGG              CC^T[ACGT]AGG
FauI          211  CCCGC                CCCGC(4/6)
HaeII        1213  AGCGCC               [GA]GCGC^[CT]
HgaI          221  GCGTC                GACGC(5/10)
HincII        199  GTCGAC               GT[CT]^[GA]AC
HindII        199  GTCGAC               GT[CT]^[GA]AC
MaeII         647  ACGT                 A^CGT
MluNI         807  TGGCCA               TGG^CCA
MroI           81  TCCGGA               T^CCGGA
MscI          807  TGGCCA               TGG^CCA
MseI          860  TTAA                 T^TAA
NspV          773  TTCGAA               TT^CGAA
PvuII         787  CAGCTG               CAG^CTG
RsaI          831  GTAC                 GT^AC
RsrII          83  CGGACCG              CG^G[AT]CCG
SalI          199  GTCGAC               G^TCGAC
SfuI          773  TTCGAA               TT^CGAA
SmlI          682  CTTGAG               C^T[CT][GA]AG
SphI          701  GCATGC               GCATG^C
Tru9I         860  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

ApaI          587  GGGCCC               GGGCC^C
             1168  GGGCCC              
BcgI           88  CGAGACCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1098  CGACAGAATTGC        
BclI          800  TGATCA               T^GATCA
              836  TGATCA              
BglI          313  GCCGCCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              472  GCCATCTCGGC         
BpmI          160  CTGGAG               CTGGAG(16/14)
              619  CTGGAG              
BseRI         571  GAGGAG               GAGGAG(10/8)
              853  GAGGAG              
BsgI          248  GTGCAG               GTGCAG(16/14)
              535  GTGCAG              
EcoRI        1093  GAATTC               G^AATTC
             1181  GAATTC              
FbaI          800  TGATCA               T^GATCA
              836  TGATCA              
HinfI         237  GAGTC                G^A[ACGT]TC
              613  GACTC               
MspA1I        787  CAGCTG               C[AC]G^C[GT]G
              990  CCGCTG              
PleI          613  GACTC                GAGTC(4/5)
              237  GAGTC               
SfaNI         274  GATGC                GCATC(5/9)
              184  GCATC               
Tsp45I        454  GTGAC                ^GT[GC]AC
              792  GTCAC               
XbaI          390  TCTAGA               T^CTAGA
             1089  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AcsI        AluI        AlwI        
ApoI        BanII       BbvI        BcnI        BfaI        
BmyI        BsaI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        Sau3AI      Sau96I      
ScrFI       StyI        TaqI        TseI        Tsp509I     
TspRI       TthHB8I     XhoII       
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