Restriction map for pF1KA0721
 (Linear) (Six-base) MAPSORT of: KIAA0721.seq  Check: 5014  from: 1  to: 1242



KIAA0721.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AccI GT'mk_AC

Cuts at:      0     200    1242 
   Size:        200    1042
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     661    1209    1242 
   Size:        661     548      33

  Fragments arranged by size: 

                 661     548      33
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     290    1242 
   Size:        290     952
 
AlwNI CAG_nnn'CTG

Cuts at:      0     310    1242 
   Size:        310     932
 
ApaI G_GGCC'C

Cuts at:      0     591    1172    1242 
   Size:        591     581      70

  Fragments arranged by size: 

                 591     581      70
 
ApoI r'AATT_y

Cuts at:      0     883    1093    1181    1242 
   Size:        883     210      88      61

  Fragments arranged by size: 

                 883     210      88      61
 
BamHI G'GATC_C

Cuts at:      0     192    1242 
   Size:        192    1050
 
BanI G'GyrC_C

Cuts at:      0     995    1242 
   Size:        995     247
 
BanII G_rGCy'C

Cuts at:      0     107     365     591    1172    1242 
   Size:        107     258     226     581      70

  Fragments arranged by size: 

                 581     258     226     107      70
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     824    1242 
   Size:        824     418
 
BbvCI CC'TCA_GC

Cuts at:      0     826    1242 
   Size:        826     416
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      77     111    1087    1121    1242 
   Size:         77      34     976      34     121

  Fragments arranged by size: 

                 976     121      77      34      34
 
BclI T'GATC_A

Cuts at:      0     800     836    1242 
   Size:        800      36     406

  Fragments arranged by size: 

                 800     406      36
 
BglI GCCn_nnn'nGGC

Cuts at:      0     319     478    1242 
   Size:        319     159     764

  Fragments arranged by size: 

                 764     319     159
 
Bme1580I G_kGCm'C

Cuts at:      0     591    1172    1242 
   Size:        591     581      70

  Fragments arranged by size: 

                 591     581      70
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     220    1242 
   Size:        220    1022
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     640    1242 
   Size:        181     459     602

  Fragments arranged by size: 

                 602     459     181
 
Bpu10I CC'TnA_GC

Cuts at:      0     826    1242 
   Size:        826     416
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     703    1242 
   Size:        703     539
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      83     157     425    1242 
   Size:         83      74     268     817

  Fragments arranged by size: 

                 817     268      83      74
 
BsaWI w'CCGG_w

Cuts at:      0      81    1242 
   Size:         81    1161
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     586     868    1242 
   Size:        586     282     374

  Fragments arranged by size: 

                 586     374     282
 
BseYI C'CCAG_C

Cuts at:      0     376     785    1242 
   Size:        376     409     457

  Fragments arranged by size: 

                 457     409     376
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     556    1242 
   Size:        269     287     686

  Fragments arranged by size: 

                 686     287     269
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     365     591    1172    1242 
   Size:        107     258     226     581      70

  Fragments arranged by size: 

                 581     258     226     107      70
 
BspEI T'CCGG_A

Cuts at:      0      81    1242 
   Size:         81    1161
 
BsrBI CCG'CTC

Cuts at:      0       5    1242 
   Size:          5    1237
 
BsrFI r'CCGG_y

Cuts at:      0      43    1242 
   Size:         43    1199
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     310    1242 
   Size:        310     932
 
BstBI TT'CG_AA

Cuts at:      0     774    1242 
   Size:        774     468
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     343     758     992    1242 
   Size:        343     415     234     250

  Fragments arranged by size: 

                 415     343     250     234
 
BstYI r'GATC_y

Cuts at:      0      78     192     952    1219    1242 
   Size:         78     114     760     267      23

  Fragments arranged by size: 

                 760     267     114      78      23
 
Bsu36I CC'TnA_GG

Cuts at:      0      72    1242 
   Size:         72    1170
 
BtgI C'CryG_G

Cuts at:      0     366    1242 
   Size:        366     876
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     529    1242 
   Size:        529     713
 
BtsI GCAGTG_nn'

Cuts at:      0     491    1242 
   Size:        491     751
 
ClaI AT'CG_AT

Cuts at:      0     629    1242 
   Size:        629     613
 
EaeI y'GGCC_r

Cuts at:      0     696     807     960    1242 
   Size:        696     111     153     282

  Fragments arranged by size: 

                 696     282     153     111
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     640     661    1209    1242 
   Size:        181     459      21     548      33

  Fragments arranged by size: 

                 548     459     181      33      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     587     588     668    1168    1169    1242 
   Size:        587       1      80     500       1      73

  Fragments arranged by size: 

                 587     500      80      73       1       1
 
EcoRI G'AATT_C

Cuts at:      0    1093    1181    1242 
   Size:       1093      88      61

  Fragments arranged by size: 

                1093      88      61
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     912    1242 
   Size:        912     330
 
HaeII r_GCGC'y

Cuts at:      0    1217    1242 
   Size:       1217      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87    1242 
   Size:         55      32    1155

  Fragments arranged by size: 

                1155      55      32
 
HincII GTy'rAC

Cuts at:      0     201    1242 
   Size:        201    1041
 
MscI TGG'CCA

Cuts at:      0     809    1242 
   Size:        809     433
 
MspA1I CmG'CkG

Cuts at:      0     789     992    1242 
   Size:        789     203     250

  Fragments arranged by size: 

                 789     250     203
 
NspI r_CATG'y

Cuts at:      0     705     720     825    1242 
   Size:        705      15     105     417

  Fragments arranged by size: 

                 705     417     105      15
 
PasI CC'CwG_GG

Cuts at:      0     584    1242 
   Size:        584     658
 
PspOMI G'GGCC_C

Cuts at:      0     587    1168    1242 
   Size:        587     581      74

  Fragments arranged by size: 

                 587     581      74
 
PvuII CAG'CTG

Cuts at:      0     789    1242 
   Size:        789     453
 
RsrII CG'GwC_CG

Cuts at:      0      84    1242 
   Size:         84    1158
 
SalI G'TCGA_C

Cuts at:      0     199    1242 
   Size:        199    1043
 
SmlI C'TyrA_G

Cuts at:      0     682    1242 
   Size:        682     560
 
SphI G_CATG'C

Cuts at:      0     705    1242 
   Size:        705     537
 
StyI C'CwwG_G

Cuts at:      0      99     446    1195    1242 
   Size:         99     347     749      47

  Fragments arranged by size: 

                 749     347      99      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     101    1150    1242 
   Size:        101    1049      92

  Fragments arranged by size: 

                1049     101      92
 
XbaI T'CTAG_A

Cuts at:      0     390    1089    1242 
   Size:        390     699     153

  Fragments arranged by size: 

                 699     390     153
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     ApaI     ApoI    BamHI     BanI
    BanII     BbsI    BbvCI     BcgI     BclI     BglI Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BseRI    BseYI     BsgI
 Bsp1286I    BspEI    BsrBI    BsrFI   BstAPI    BstBI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI     EaeI  Eco57MI EcoO109I
    EcoRI     FalI    HaeII    Hin4I   HincII     MscI   MspA1I     NspI
     PasI   PspOMI    PvuII    RsrII     SalI     SmlI     SphI     StyI
    TaqII     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BbeI    BciVI    BfrBI    BglII     BlpI    BmgBI
     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrDI    BsrGI   BssHII
    BssSI   BstEII  BstZ17I    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI     SacI
    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     XmnI     ZraI
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