Restriction map for pF1KA0610
 (Linear) (Six-base) MAPSORT of: KIAA0610.seq  Check: 3094  from: 1  to: 1998



KIAA0610.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     164     474    1998 
   Size:        164     310    1524

  Fragments arranged by size: 

                1524     310     164
 
Acc65I G'GTAC_C

Cuts at:      0     737    1998 
   Size:        737    1261
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     437     557     790    1998 
   Size:        304     133     120     233    1208

  Fragments arranged by size: 

                1208     304     233     133     120
 
AflIII A'CryG_T

Cuts at:      0     362     878    1533    1998 
   Size:        362     516     655     465

  Fragments arranged by size: 

                 655     516     465     362
 
AleI CACnn'nnGTG

Cuts at:      0    1102    1998 
   Size:       1102     896
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     927    1698    1998 
   Size:        927     771     300

  Fragments arranged by size: 

                 927     771     300
 
AlwNI CAG_nnn'CTG

Cuts at:      0     205     460     571    1479    1711    1998 
   Size:        205     255     111     908     232     287

  Fragments arranged by size: 

                 908     287     255     232     205     111
 
ApaLI G'TGCA_C

Cuts at:      0     719    1998 
   Size:        719    1279
 
ApoI r'AATT_y

Cuts at:      0     278     346    1039    1998 
   Size:        278      68     693     959

  Fragments arranged by size: 

                 959     693     278      68
 
AvaI C'yCGr_G

Cuts at:      0     796    1998 
   Size:        796    1202
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     712     745    1806    1839    1998 
   Size:        712      33    1061      33     159

  Fragments arranged by size: 

                1061     712     159      33      33
 
BanI G'GyrC_C

Cuts at:      0     327     737    1998 
   Size:        327     410    1261

  Fragments arranged by size: 

                1261     410     327
 
BanII G_rGCy'C

Cuts at:      0     965    1998 
   Size:        965    1033
 
BbvCI CC'TCA_GC

Cuts at:      0     407    1998 
   Size:        407    1591
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     564     740    1998 
   Size:        564     176    1258

  Fragments arranged by size: 

                1258     564     176
 
BfrBI ATG'CAT

Cuts at:      0    1685    1998 
   Size:       1685     313
 
BglII A'GATC_T

Cuts at:      0     844    1998 
   Size:        844    1154
 
Bme1580I G_kGCm'C

Cuts at:      0     332     723    1998 
   Size:        332     391    1275

  Fragments arranged by size: 

                1275     391     332
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     988    1998 
   Size:        988    1010
 
Bpu10I CC'TnA_GC

Cuts at:      0     407    1998 
   Size:        407    1591
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     512     652    1894    1998 
   Size:        512     140    1242     104

  Fragments arranged by size: 

                1242     512     140     104
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     628    1998 
   Size:        628    1370
 
BsaWI w'CCGG_w

Cuts at:      0     849    1998 
   Size:        849    1149
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     423     453    1853    1883    1998 
   Size:        423      30    1400      30     115

  Fragments arranged by size: 

                1400     423     115      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     395     500     515     610    1059    1469    1589    1998 
   Size:        395     105      15      95     449     410     120     409

  Fragments arranged by size: 

                 449     410     409     395     120     105      95      15
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     446     467    1694    1998 
   Size:        446      21    1227     304

  Fragments arranged by size: 

                1227     446     304      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     723     965    1998 
   Size:        723     242    1033

  Fragments arranged by size: 

                1033     723     242
 
Bsp1286I G_dGCh'C

Cuts at:      0     332     723     965    1998 
   Size:        332     391     242    1033

  Fragments arranged by size: 

                1033     391     332     242
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      36     164     474    1998 
   Size:         36     128     310    1524

  Fragments arranged by size: 

                1524     310     128      36
 
BsrBI CCG'CTC

Cuts at:      0    1374    1998 
   Size:       1374     624
 
BsrDI GCAATG_nn'

Cuts at:      0    1838    1998 
   Size:       1838     160
 
BsrGI T'GTAC_A

Cuts at:      0    1713    1998 
   Size:       1713     285
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     460    1998 
   Size:        460    1538
 
BstBI TT'CG_AA

Cuts at:      0     744    1998 
   Size:        744    1254
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1051    1998 
   Size:       1051     947
 
BstYI r'GATC_y

Cuts at:      0     844     972    1998 
   Size:        844     128    1026

  Fragments arranged by size: 

                1026     844     128
 
Bsu36I CC'TnA_GG

Cuts at:      0     639    1998 
   Size:        639    1359
 
BtgI C'CryG_G

Cuts at:      0    1397    1998 
   Size:       1397     601
 
EcoICRI GAG'CTC

Cuts at:      0     963    1998 
   Size:        963    1035
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     437     557     790     988    1998 
   Size:        304     133     120     233     198    1010

  Fragments arranged by size: 

                1010     304     233     198     133     120
 
EcoO109I rG'GnC_Cy

Cuts at:      0     202    1998 
   Size:        202    1796
 
EcoRI G'AATT_C

Cuts at:      0    1039    1998 
   Size:       1039     959
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      84    1998 
   Size:         84    1914
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     143     175     373     405     568     600    1579    1611 
   Size:        143      32     198      32     163      32     979      32

Cuts at:   1611    1853    1885    1896    1928    1998 
   Size:        242      32      11      32      70

  Fragments arranged by size: 

                 979     242     198     163     143      70      32      32
                  32      32      32      32      11
 
HincII GTy'rAC

Cuts at:      0      81    1191    1692    1935    1998 
   Size:         81    1110     501     243      63

  Fragments arranged by size: 

                1110     501     243      81      63
 
HpaI GTT'AAC

Cuts at:      0      81    1191    1692    1998 
   Size:         81    1110     501     306

  Fragments arranged by size: 

                1110     501     306      81
 
KpnI G_GTAC'C

Cuts at:      0     741    1998 
   Size:        741    1257
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     570    1094    1494    1998 
   Size:        570     524     400     504

  Fragments arranged by size: 

                 570     524     504     400
 
MslI CAynn'nnrTG

Cuts at:      0    1102    1998 
   Size:       1102     896
 
MspA1I CmG'CkG

Cuts at:      0    1414    1998 
   Size:       1414     584
 
NsiI A_TGCA'T

Cuts at:      0    1687    1998 
   Size:       1687     311
 
NspI r_CATG'y

Cuts at:      0     366     415     882    1537    1998 
   Size:        366      49     467     655     461

  Fragments arranged by size: 

                 655     467     461     366      49
 
PciI A'CATG_T

Cuts at:      0     362     878    1533    1998 
   Size:        362     516     655     465

  Fragments arranged by size: 

                 655     516     465     362
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      19    1770    1998 
   Size:         19    1751     228

  Fragments arranged by size: 

                1751     228      19
 
PpuMI rG'GwC_Cy

Cuts at:      0     202    1998 
   Size:        202    1796
 
PstI C_TGCA'G

Cuts at:      0     311     708    1998 
   Size:        311     397    1290

  Fragments arranged by size: 

                1290     397     311
 
PvuII CAG'CTG

Cuts at:      0    1414    1998 
   Size:       1414     584
 
SacI G_AGCT'C

Cuts at:      0     965    1998 
   Size:        965    1033
 
SbfI CC_TGCA'GG

Cuts at:      0     708    1998 
   Size:        708    1290
 
SfcI C'TryA_G

Cuts at:      0     253     307     704    1448    1499    1998 
   Size:        253      54     397     744      51     499

  Fragments arranged by size: 

                 744     499     397     253      54      51
 
SmaI CCC'GGG

Cuts at:      0     798    1998 
   Size:        798    1200
 
SmlI C'TyrA_G

Cuts at:      0     527     631    1873    1998 
   Size:        527     104    1242     125

  Fragments arranged by size: 

                1242     527     125     104
 
SphI G_CATG'C

Cuts at:      0     415    1998 
   Size:        415    1583
 
SspI AAT'ATT

Cuts at:      0    1809    1998 
   Size:       1809     189
 
StuI AGG'CCT

Cuts at:      0      67    1998 
   Size:         67    1931
 
StyI C'CwwG_G

Cuts at:      0    1119    1421    1998 
   Size:       1119     302     577

  Fragments arranged by size: 

                1119     577     302
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     197    1287    1998 
   Size:        197    1090     711

  Fragments arranged by size: 

                1090     711     197
 
TatI w'GTAC_w

Cuts at:      0     681     862    1713    1998 
   Size:        681     181     851     285

  Fragments arranged by size: 

                 851     681     285     181
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     423     455     902     934    1998 
   Size:        423      32     447      32    1064

  Fragments arranged by size: 

                1064     447     423      32      32
 
XbaI T'CTAG_A

Cuts at:      0     282    1998 
   Size:        282    1716
 
XmaI C'CCGG_G

Cuts at:      0     796    1998 
   Size:        796    1202
 
XmnI GAAnn'nnTTC

Cuts at:      0     567    1998 
   Size:        567    1431
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AleI     AlfI    AlwNI    ApaLI
     ApoI     AvaI     BaeI     BanI    BanII    BbvCI    BciVI    BfrBI
    BglII Bme1580I     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI
    BseRI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrGI
   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI    Hin4I   HincII     HpaI     KpnI     MmeI
     MslI   MspA1I     NsiI     NspI     PciI    PflMI    PpuMI     PstI
    PvuII     SacI     SbfI     SfcI     SmaI     SmlI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI     AloI
     ApaI     AscI     AseI    AsiSI    AvrII    BamHI     BbeI     BbsI
     BcgI     BclI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI
    BsaAI    BsaBI    BsaHI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrFI   BssHII    BssSI   BstEII  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII
  HindIII     KasI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SwaI
  Tth111I     XcmI     XhoI     ZraI
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