Restriction map for pF1KA0589
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AccI        AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AocI        ApoI        AseI        
AsnI        AviII       AvrII       BbrPI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaI        BseAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BsrGI       Bst1107I    BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FspI        
HincII      HindII      HindIII     HpaI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NdeI        NotI        NruI        NsiI        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SalI        SbfI        ScaI        SexAI       
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       Tsp509I     
Van91I      XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        1626  GACGTC               GACGT^C
Acc65I         12  GGTACC               G^GTACC
AhdI         1261  GACACGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        967  GTGCAC               G^TGCAC
AlwNI         595  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         967  GTGCAC               G^TGCAC
AscI          782  GGCGCGCC             GG^CGCGCC
Asp700I       807  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I        12  GGTACC               G^GTACC
AspEI        1261  GACACGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1578  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          156  TGGCCA               TGG^CCA
BbsI          216  GAAGAC               GAAGAC(2/6)
BbvCI          34  GCTGAGG              CCTCAGC(-5/-2)
BpuAI         216  GAAGAC               GAAGAC(2/6)
BsaAI         322  TACGTG               [CT]AC^GT[GA]
BsaWI          15  ACCGGA               [AT]^CCGG[AT]
BsmAI        1624  GAGAC                GTCTC(1/5)
BsmBI        1624  GAGACG               CGTCTC(1/5)
BssSI         985  CACGAG               CACGAG(-5/-1)
BstBI        1546  TTCGAA               TT^CGAA
DrdI          913  GACTGCCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1261  GACACGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI         1510  GGCCGGCC             GGCCGG^CC
KpnI           12  GGTACC               GGTAC^C
MaeIII        535  GTCAC                ^GT[ACGT]AC
MluNI         156  TGGCCA               TGG^CCA
MscI          156  TGGCCA               TGG^CCA
NaeI         1511  GCCGGC               GCC^GGC
NgoMIV       1511  GCCGGC               G^CCGGC
NheI         1389  GCTAGC               G^CTAGC
NspI          848  ACATGC               [GA]CATG^[CT]
NspV         1546  TTCGAA               TT^CGAA
PflFI        1578  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         827  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         250  CAGCTG               CAG^CTG
SapI          807  GAAGAGC              GCTCTTC(1/4)
SfiI         1971  GGCCCCCCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         1546  TTCGAA               TT^CGAA
SmaI          507  CCCGGG               CCC^GGG
TfiI         1114  GAATC                G^A[AT]TC
Tsp45I        535  GTCAC                ^GT[GC]AC
Tth111I      1578  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         507  CCCGGG               C^CCGGG
XmaI          507  CCCGGG               C^CCGGG
XmnI          807  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

Aor51HI        31  AGCGCT               AGC^GCT
             1298  AGCGCT              
AvaI          507  CCCGGG               C^[CT]CG[GA]G
              853  CCCGAG              
BamHI         446  GGATCC               G^GATCC
             1136  GGATCC              
BfaI         1390  CTAG                 C^TAG
             1442  CTAG                
BsiEI        1152  CGGCCG               CG[GA][CT]^CG
             1802  CGGCCG              
BsoBI         507  CCCGGG               C^[CT]CG[GA]G
              853  CCCGAG              
BspHI         710  TCATGA               T^CATGA
             1313  TCATGA              
BsrDI        1569  CATTGC               GCAATG(2/0)
              479  GCAATG              
BsrFI        1511  GCCGGC               [GA]^CCGG[CT]
             1804  GCCGGT              
BssHII        783  GCGCGC               G^CGCGC
             1096  GCGCGC              
BstAPI        594  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              843  GCAGGACATGC         
BstXI         245  CCATCCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1064  CCACGGCCATGG        
Cfr10I       1511  GCCGGC               [GA]^CCGG[CT]
             1804  GCCGGT              
EagI         1152  CGGCCG               C^GGCCG
             1802  CGGCCG              
EclXI        1152  CGGCCG               C^GGCCG
             1802  CGGCCG              
Eco47III       31  AGCGCT               AGC^GCT
             1298  AGCGCT              
Eco52I       1152  CGGCCG               C^GGCCG
             1802  CGGCCG              
EcoT14I       159  CCATGG               C^C[AT][AT]GG
             1070  CCATGG              
HphI          365  TCACC                GGTGA(8/7)
              536  TCACC               
MaeI         1390  CTAG                 C^TAG
             1442  CTAG                
MslI          178  CATCGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1065  CACGGCCATG          
NcoI          159  CCATGG               C^CATGG
             1070  CCATGG              
PleI         1578  GACTC                GAGTC(4/5)
             1075  GAGTC               
RcaI          710  TCATGA               T^CATGA
             1313  TCATGA              
SacI          282  GAGCTC               GAGCT^C
              436  GAGCTC              
SmlI          748  CTCAAG               C^T[CT][GA]AG
              760  CTCAAG              
StuI         1844  AGGCCT               AGG^CCT
             1877  AGGCCT              
StyI          159  CCATGG               C^C[AT][AT]GG
             1070  CCATGG              
TspRI         516  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1727  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AspHI       AvaII       BanI        
BanII       BbeI        BbvI        BcnI        BglI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BspMI       BsrBI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       ItaI        KasI        Ksp632I     
KspI        MaeII       MboI        MboII       MflI        
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NdeII       NlaIII      
NlaIV       PstI        RsaI        SacII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
TaqI        TseI        TthHB8I     XhoII       
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